Amperometric enzyme electrode for free cholesterol determination prepared with cholesterol oxidase immobilized in poly(vinylferrocenium) film
The steady state amperometric measurements of free cholesterol were performed by an enzyme electrode which was developed through electrostatic immobilization of the cholesterol oxidase in poly(vinylferrocenium) perchlorate, PVF +ClO 4 −, film that was coated on a Pt electrode. Amperometric measureme...
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| Veröffentlicht in: | Enzyme and microbial technology 2007-01, Vol.40 (2), p.262-265 |
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| container_title | Enzyme and microbial technology |
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| creator | ÖZER, B. Cem ÖZYÖRÜK, Haluk CELEBI, Serdar S YILDIZ, Atilla |
| description | The steady state amperometric measurements of free cholesterol were performed by an enzyme electrode which was developed through electrostatic immobilization of the cholesterol oxidase in poly(vinylferrocenium) perchlorate, PVF
+ClO
4
−, film that was coated on a Pt electrode. Amperometric measurements were performed at an applied potential of 0.7
V versus SCE. The effects of enzyme concentration, polymer film thickness, working temperature, dissolved oxygen gas and pH of solution on the response electrode were elucidated. The activation energy and the apparent Michaelis–Menten constant were calculated for enzyme electrode system. The linear working range, the sensitivity and the stability of the biosensor were also determined. The mechanism for the operation of the enzyme electrode was proposed. |
| doi_str_mv | 10.1016/j.enzmictec.2006.04.007 |
| format | Article |
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+ClO
4
−, film that was coated on a Pt electrode. Amperometric measurements were performed at an applied potential of 0.7
V versus SCE. The effects of enzyme concentration, polymer film thickness, working temperature, dissolved oxygen gas and pH of solution on the response electrode were elucidated. The activation energy and the apparent Michaelis–Menten constant were calculated for enzyme electrode system. The linear working range, the sensitivity and the stability of the biosensor were also determined. The mechanism for the operation of the enzyme electrode was proposed.</description><identifier>ISSN: 0141-0229</identifier><identifier>EISSN: 1879-0909</identifier><identifier>DOI: 10.1016/j.enzmictec.2006.04.007</identifier><identifier>CODEN: EMTED2</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Amperometric enzyme electrode ; Biological and medical sciences ; Biotechnology ; Cholesterol oxidase ; Free cholesterol biosensor ; Fundamental and applied biological sciences. Psychology ; Poly(vinylferrocenium)</subject><ispartof>Enzyme and microbial technology, 2007-01, Vol.40 (2), p.262-265</ispartof><rights>2006 Elsevier Inc.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-62fb90bbb2b902453012cab438968fa8789169d0f2a220d9cbc995add3f742b93</citedby><cites>FETCH-LOGICAL-c376t-62fb90bbb2b902453012cab438968fa8789169d0f2a220d9cbc995add3f742b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.enzmictec.2006.04.007$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18400798$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>ÖZER, B. Cem</creatorcontrib><creatorcontrib>ÖZYÖRÜK, Haluk</creatorcontrib><creatorcontrib>CELEBI, Serdar S</creatorcontrib><creatorcontrib>YILDIZ, Atilla</creatorcontrib><title>Amperometric enzyme electrode for free cholesterol determination prepared with cholesterol oxidase immobilized in poly(vinylferrocenium) film</title><title>Enzyme and microbial technology</title><description>The steady state amperometric measurements of free cholesterol were performed by an enzyme electrode which was developed through electrostatic immobilization of the cholesterol oxidase in poly(vinylferrocenium) perchlorate, PVF
+ClO
4
−, film that was coated on a Pt electrode. Amperometric measurements were performed at an applied potential of 0.7
V versus SCE. The effects of enzyme concentration, polymer film thickness, working temperature, dissolved oxygen gas and pH of solution on the response electrode were elucidated. The activation energy and the apparent Michaelis–Menten constant were calculated for enzyme electrode system. The linear working range, the sensitivity and the stability of the biosensor were also determined. The mechanism for the operation of the enzyme electrode was proposed.</description><subject>Amperometric enzyme electrode</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cholesterol oxidase</subject><subject>Free cholesterol biosensor</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Poly(vinylferrocenium)</subject><issn>0141-0229</issn><issn>1879-0909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkc9u1DAQxi0EEkvLM-ALCA5JJ06axMdVVWilSlzK2XLssTorOw52trB9B965LluBOHH6pNHvmz_fMPaugbqBpj_b1Tg_BDIrmloA9DV0NcDwgm2acZAVSJAv2QaarqlACPmavcl5B1AKHWzYr21YMMWAayLDS6dDQI4ezZqiRe5i4i4hcnMXPea1oJ5bLBpo1ivFmS8JF53Q8h-03v3DxZ9kdUZOIcSJPD0UiIoh-sPHe5oP3mFK0eBM-_CJO_LhlL1y2md8-6wn7Nvny9uLq-rm65fri-1NZdqhX6teuEnCNE2iiOjOW2iE0VPXjrIfnR6HUTa9tOCEFgKsNJOR8lxb27qhK572hH049l1S_L4v-6pA2aD3esa4z0pAK-QIQwGHI2hSzDmhU0uioNNBNaCe4lc79Sd-9RS_gk7Bb-f75xE6G-1d0rOh_Nc-doWSY-G2Rw7LvfeESWVDOBu0lMoXlI3031mPyY-kaw</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>ÖZER, B. Cem</creator><creator>ÖZYÖRÜK, Haluk</creator><creator>CELEBI, Serdar S</creator><creator>YILDIZ, Atilla</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20070101</creationdate><title>Amperometric enzyme electrode for free cholesterol determination prepared with cholesterol oxidase immobilized in poly(vinylferrocenium) film</title><author>ÖZER, B. Cem ; ÖZYÖRÜK, Haluk ; CELEBI, Serdar S ; YILDIZ, Atilla</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-62fb90bbb2b902453012cab438968fa8789169d0f2a220d9cbc995add3f742b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Amperometric enzyme electrode</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cholesterol oxidase</topic><topic>Free cholesterol biosensor</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Poly(vinylferrocenium)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ÖZER, B. Cem</creatorcontrib><creatorcontrib>ÖZYÖRÜK, Haluk</creatorcontrib><creatorcontrib>CELEBI, Serdar S</creatorcontrib><creatorcontrib>YILDIZ, Atilla</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Enzyme and microbial technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ÖZER, B. Cem</au><au>ÖZYÖRÜK, Haluk</au><au>CELEBI, Serdar S</au><au>YILDIZ, Atilla</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amperometric enzyme electrode for free cholesterol determination prepared with cholesterol oxidase immobilized in poly(vinylferrocenium) film</atitle><jtitle>Enzyme and microbial technology</jtitle><date>2007-01-01</date><risdate>2007</risdate><volume>40</volume><issue>2</issue><spage>262</spage><epage>265</epage><pages>262-265</pages><issn>0141-0229</issn><eissn>1879-0909</eissn><coden>EMTED2</coden><abstract>The steady state amperometric measurements of free cholesterol were performed by an enzyme electrode which was developed through electrostatic immobilization of the cholesterol oxidase in poly(vinylferrocenium) perchlorate, PVF
+ClO
4
−, film that was coated on a Pt electrode. Amperometric measurements were performed at an applied potential of 0.7
V versus SCE. The effects of enzyme concentration, polymer film thickness, working temperature, dissolved oxygen gas and pH of solution on the response electrode were elucidated. The activation energy and the apparent Michaelis–Menten constant were calculated for enzyme electrode system. The linear working range, the sensitivity and the stability of the biosensor were also determined. The mechanism for the operation of the enzyme electrode was proposed.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><doi>10.1016/j.enzmictec.2006.04.007</doi><tpages>4</tpages></addata></record> |
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| ispartof | Enzyme and microbial technology, 2007-01, Vol.40 (2), p.262-265 |
| issn | 0141-0229 1879-0909 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Amperometric enzyme electrode Biological and medical sciences Biotechnology Cholesterol oxidase Free cholesterol biosensor Fundamental and applied biological sciences. Psychology Poly(vinylferrocenium) |
| title | Amperometric enzyme electrode for free cholesterol determination prepared with cholesterol oxidase immobilized in poly(vinylferrocenium) film |
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