Rapid detection of the neonicotinoid insecticide imidacloprid using a quenchbody assay
Contamination of the land and water by neonicotinoid insecticide residues is currently a severe environmental problem. However, the traditional methods for pesticide residue analysis are time consuming and laborious. To tackle this problem, here we describe a novel quenchbody (Q-body) immunoassay re...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2018-07, Vol.410 (17), p.4219-4226 |
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| creator | Zhao, Shitao Dong, Jinhua Jeong, Hee-Jin Okumura, Koichi Ueda, Hiroshi |
| description | Contamination of the land and water by neonicotinoid insecticide residues is currently a severe environmental problem. However, the traditional methods for pesticide residue analysis are time consuming and laborious. To tackle this problem, here we describe a novel quenchbody (Q-body) immunoassay reagent that allows the rapid and sensitive detection of imidacloprid, one of the most frequently used neonicotinoid pesticides, in aqueous solution. A Q-body comprises an antibody Fab fragment that is site-specifically labeled with a fluorescent dye. The Fab fragment quenches the dye with its internal tryptophan residues via photoinduced electron transfer. The subsequent addition of imidacloprid stabilizes the antibody structure and displaces the quenched dye to the outside of the protein, resulting in increased fluorescence. The constructed Q-body assay exhibited a high dynamic range and a low limit of detection (10 ng mL
−1
), and the entire assay procedure could be completed in a few minutes. The assay showed a low cross-reactivity with possible interfering analogous compounds, indicating that it has a good selectivity. Hence, the developed Q-body assay has excellent potential as a universal technology for monitoring neonicotinoid residues in environmental and food samples.
Graphical abstract
A novel quenchbody (Q-body) immunoassay reagent that allows the rapid and sensitive detection of imidacloprid, one of the most frequently used neonicotinoid pesticides, in aqueous solution was developed. The addition of imidacloprid stabilizes the Q-body structure and displaces the quenched dye to the outside of the protein, resulting in increased fluorescence. The constructed Q-body assay exhibited a high dynamic range and a low limit of detection (10 ng mL
−1
), and completed in a few minutes. |
| doi_str_mv | 10.1007/s00216-018-1074-y |
| format | Article |
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−1
), and the entire assay procedure could be completed in a few minutes. The assay showed a low cross-reactivity with possible interfering analogous compounds, indicating that it has a good selectivity. Hence, the developed Q-body assay has excellent potential as a universal technology for monitoring neonicotinoid residues in environmental and food samples.
Graphical abstract
A novel quenchbody (Q-body) immunoassay reagent that allows the rapid and sensitive detection of imidacloprid, one of the most frequently used neonicotinoid pesticides, in aqueous solution was developed. The addition of imidacloprid stabilizes the Q-body structure and displaces the quenched dye to the outside of the protein, resulting in increased fluorescence. The constructed Q-body assay exhibited a high dynamic range and a low limit of detection (10 ng mL
−1
), and completed in a few minutes.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-018-1074-y</identifier><identifier>PMID: 29704031</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analytical Chemistry ; Aqueous solutions ; Bioassay ; Biochemistry ; Biological Assay - methods ; Characterization and Evaluation of Materials ; Chemical properties ; Chemistry ; Chemistry and Materials Science ; Construction ; Cross-reactivity ; Dyes ; Dynamic range ; Electron transfer ; Environmental monitoring ; Enzyme-Linked Immunosorbent Assay ; Fab ; Fluorescence ; Fluorescent dyes ; Fluorescent indicators ; Food contamination ; Food Science ; Imidacloprid ; Immunoassay ; Insecticides ; Insecticides - analysis ; Laboratory Medicine ; Land pollution ; Limit of Detection ; Methods ; Monitoring/Environmental Analysis ; Neonicotinoids - analysis ; Nitro Compounds - analysis ; Pesticide residues ; Pesticides ; Reagents ; Research Paper ; Residues ; Time Factors ; Tryptophan</subject><ispartof>Analytical and bioanalytical chemistry, 2018-07, Vol.410 (17), p.4219-4226</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Analytical and Bioanalytical Chemistry is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-73587928ae5ec424d214e06395a134fd3402f5646449587c4272ea6bccc739d93</citedby><cites>FETCH-LOGICAL-c514t-73587928ae5ec424d214e06395a134fd3402f5646449587c4272ea6bccc739d93</cites><orcidid>0000-0001-8849-4217</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-018-1074-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-018-1074-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29704031$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Shitao</creatorcontrib><creatorcontrib>Dong, Jinhua</creatorcontrib><creatorcontrib>Jeong, Hee-Jin</creatorcontrib><creatorcontrib>Okumura, Koichi</creatorcontrib><creatorcontrib>Ueda, Hiroshi</creatorcontrib><title>Rapid detection of the neonicotinoid insecticide imidacloprid using a quenchbody assay</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Contamination of the land and water by neonicotinoid insecticide residues is currently a severe environmental problem. However, the traditional methods for pesticide residue analysis are time consuming and laborious. To tackle this problem, here we describe a novel quenchbody (Q-body) immunoassay reagent that allows the rapid and sensitive detection of imidacloprid, one of the most frequently used neonicotinoid pesticides, in aqueous solution. A Q-body comprises an antibody Fab fragment that is site-specifically labeled with a fluorescent dye. The Fab fragment quenches the dye with its internal tryptophan residues via photoinduced electron transfer. The subsequent addition of imidacloprid stabilizes the antibody structure and displaces the quenched dye to the outside of the protein, resulting in increased fluorescence. The constructed Q-body assay exhibited a high dynamic range and a low limit of detection (10 ng mL
−1
), and the entire assay procedure could be completed in a few minutes. The assay showed a low cross-reactivity with possible interfering analogous compounds, indicating that it has a good selectivity. Hence, the developed Q-body assay has excellent potential as a universal technology for monitoring neonicotinoid residues in environmental and food samples.
Graphical abstract
A novel quenchbody (Q-body) immunoassay reagent that allows the rapid and sensitive detection of imidacloprid, one of the most frequently used neonicotinoid pesticides, in aqueous solution was developed. The addition of imidacloprid stabilizes the Q-body structure and displaces the quenched dye to the outside of the protein, resulting in increased fluorescence. The constructed Q-body assay exhibited a high dynamic range and a low limit of detection (10 ng mL
−1
), and completed in a few minutes.</description><subject>Analytical Chemistry</subject><subject>Aqueous solutions</subject><subject>Bioassay</subject><subject>Biochemistry</subject><subject>Biological Assay - methods</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemical properties</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Construction</subject><subject>Cross-reactivity</subject><subject>Dyes</subject><subject>Dynamic range</subject><subject>Electron transfer</subject><subject>Environmental monitoring</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fab</subject><subject>Fluorescence</subject><subject>Fluorescent dyes</subject><subject>Fluorescent indicators</subject><subject>Food contamination</subject><subject>Food Science</subject><subject>Imidacloprid</subject><subject>Immunoassay</subject><subject>Insecticides</subject><subject>Insecticides - analysis</subject><subject>Laboratory Medicine</subject><subject>Land pollution</subject><subject>Limit of Detection</subject><subject>Methods</subject><subject>Monitoring/Environmental Analysis</subject><subject>Neonicotinoids - analysis</subject><subject>Nitro Compounds - analysis</subject><subject>Pesticide residues</subject><subject>Pesticides</subject><subject>Reagents</subject><subject>Research Paper</subject><subject>Residues</subject><subject>Time 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Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Shitao</au><au>Dong, Jinhua</au><au>Jeong, Hee-Jin</au><au>Okumura, Koichi</au><au>Ueda, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid detection of the neonicotinoid insecticide imidacloprid using a quenchbody assay</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2018-07-01</date><risdate>2018</risdate><volume>410</volume><issue>17</issue><spage>4219</spage><epage>4226</epage><pages>4219-4226</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Contamination of the land and water by neonicotinoid insecticide residues is currently a severe environmental problem. However, the traditional methods for pesticide residue analysis are time consuming and laborious. To tackle this problem, here we describe a novel quenchbody (Q-body) immunoassay reagent that allows the rapid and sensitive detection of imidacloprid, one of the most frequently used neonicotinoid pesticides, in aqueous solution. A Q-body comprises an antibody Fab fragment that is site-specifically labeled with a fluorescent dye. The Fab fragment quenches the dye with its internal tryptophan residues via photoinduced electron transfer. The subsequent addition of imidacloprid stabilizes the antibody structure and displaces the quenched dye to the outside of the protein, resulting in increased fluorescence. The constructed Q-body assay exhibited a high dynamic range and a low limit of detection (10 ng mL
−1
), and the entire assay procedure could be completed in a few minutes. The assay showed a low cross-reactivity with possible interfering analogous compounds, indicating that it has a good selectivity. Hence, the developed Q-body assay has excellent potential as a universal technology for monitoring neonicotinoid residues in environmental and food samples.
Graphical abstract
A novel quenchbody (Q-body) immunoassay reagent that allows the rapid and sensitive detection of imidacloprid, one of the most frequently used neonicotinoid pesticides, in aqueous solution was developed. The addition of imidacloprid stabilizes the Q-body structure and displaces the quenched dye to the outside of the protein, resulting in increased fluorescence. The constructed Q-body assay exhibited a high dynamic range and a low limit of detection (10 ng mL
−1
), and completed in a few minutes.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29704031</pmid><doi>10.1007/s00216-018-1074-y</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-8849-4217</orcidid></addata></record> |
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| ispartof | Analytical and bioanalytical chemistry, 2018-07, Vol.410 (17), p.4219-4226 |
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| subjects | Analytical Chemistry Aqueous solutions Bioassay Biochemistry Biological Assay - methods Characterization and Evaluation of Materials Chemical properties Chemistry Chemistry and Materials Science Construction Cross-reactivity Dyes Dynamic range Electron transfer Environmental monitoring Enzyme-Linked Immunosorbent Assay Fab Fluorescence Fluorescent dyes Fluorescent indicators Food contamination Food Science Imidacloprid Immunoassay Insecticides Insecticides - analysis Laboratory Medicine Land pollution Limit of Detection Methods Monitoring/Environmental Analysis Neonicotinoids - analysis Nitro Compounds - analysis Pesticide residues Pesticides Reagents Research Paper Residues Time Factors Tryptophan |
| title | Rapid detection of the neonicotinoid insecticide imidacloprid using a quenchbody assay |
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