Comparison of Carba NP-Direct, Carbapenem Inactivation Method, and β-CARBA Tests for Detection of Carbapenemase Production in Enterobacteriaceae
Rapid and accurate detection of carbapenemase-producing isolates are extremely important for management of antimicrobial therapy and the implementation of infection control measures. We evaluated the performance of Carba NP-direct, carbapenem inactivation method (CIM), and the commercial β-CARBA tes...
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| Veröffentlicht in: | Microbial drug resistance (Larchmont, N.Y.) N.Y.), 2019-01, Vol.25 (1), p.97-102 |
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| container_title | Microbial drug resistance (Larchmont, N.Y.) |
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| creator | Bayraktar, Banu Barış, Ayşe Malkoçoğlu, Gülşah Erdemir, Duygu Kına, Nur |
| description | Rapid and accurate detection of carbapenemase-producing isolates are extremely important for management of antimicrobial therapy and the implementation of infection control measures. We evaluated the performance of Carba NP-direct, carbapenem inactivation method (CIM), and the commercial β-CARBA tests for detection of carbapenemase production in
Enterobacteriaceae
.
Enterobacteriaceae
isolates with previously characterized carbapenemase types (
n
= 110) and non-carbapenemase-producing
Escherichia coli
(
n
= 15) isolates were tested. Sensitivities of Carba NP-direct, CIM, and β-CARBA tests were 99.0%, 92.7%, and 93.6%, respectively, while specificity was 100% for all three tests. For β-CARBA test, a 60-min incubation time instead of 30 increased the sensitivity to 98.1%, and lessened false negativity, particularly with OXA-48-like producers. Our results showed that Carba NP-direct, CIM, and β-CARBA tests are useful tools for the reliable detection of carbapenemase activity in enterobacterial isolates. Carba NP-direct is a simple, rapid, and low-cost test for routine use. |
| doi_str_mv | 10.1089/mdr.2017.0427 |
| format | Article |
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Enterobacteriaceae
.
Enterobacteriaceae
isolates with previously characterized carbapenemase types (
n
= 110) and non-carbapenemase-producing
Escherichia coli
(
n
= 15) isolates were tested. Sensitivities of Carba NP-direct, CIM, and β-CARBA tests were 99.0%, 92.7%, and 93.6%, respectively, while specificity was 100% for all three tests. For β-CARBA test, a 60-min incubation time instead of 30 increased the sensitivity to 98.1%, and lessened false negativity, particularly with OXA-48-like producers. Our results showed that Carba NP-direct, CIM, and β-CARBA tests are useful tools for the reliable detection of carbapenemase activity in enterobacterial isolates. Carba NP-direct is a simple, rapid, and low-cost test for routine use.</description><identifier>ISSN: 1076-6294</identifier><identifier>EISSN: 1931-8448</identifier><identifier>DOI: 10.1089/mdr.2017.0427</identifier><identifier>PMID: 29694266</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc., publishers</publisher><subject>Epidemiology</subject><ispartof>Microbial drug resistance (Larchmont, N.Y.), 2019-01, Vol.25 (1), p.97-102</ispartof><rights>2019, Mary Ann Liebert, Inc., publishers</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-9729a1c208c2c09c2c4b8c3075b7dc41850902aec17c65805a1d91bc0b00aa993</citedby><cites>FETCH-LOGICAL-c337t-9729a1c208c2c09c2c4b8c3075b7dc41850902aec17c65805a1d91bc0b00aa993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29694266$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bayraktar, Banu</creatorcontrib><creatorcontrib>Barış, Ayşe</creatorcontrib><creatorcontrib>Malkoçoğlu, Gülşah</creatorcontrib><creatorcontrib>Erdemir, Duygu</creatorcontrib><creatorcontrib>Kına, Nur</creatorcontrib><title>Comparison of Carba NP-Direct, Carbapenem Inactivation Method, and β-CARBA Tests for Detection of Carbapenemase Production in Enterobacteriaceae</title><title>Microbial drug resistance (Larchmont, N.Y.)</title><addtitle>Microb Drug Resist</addtitle><description>Rapid and accurate detection of carbapenemase-producing isolates are extremely important for management of antimicrobial therapy and the implementation of infection control measures. We evaluated the performance of Carba NP-direct, carbapenem inactivation method (CIM), and the commercial β-CARBA tests for detection of carbapenemase production in
Enterobacteriaceae
.
Enterobacteriaceae
isolates with previously characterized carbapenemase types (
n
= 110) and non-carbapenemase-producing
Escherichia coli
(
n
= 15) isolates were tested. Sensitivities of Carba NP-direct, CIM, and β-CARBA tests were 99.0%, 92.7%, and 93.6%, respectively, while specificity was 100% for all three tests. For β-CARBA test, a 60-min incubation time instead of 30 increased the sensitivity to 98.1%, and lessened false negativity, particularly with OXA-48-like producers. Our results showed that Carba NP-direct, CIM, and β-CARBA tests are useful tools for the reliable detection of carbapenemase activity in enterobacterial isolates. Carba NP-direct is a simple, rapid, and low-cost test for routine use.</description><subject>Epidemiology</subject><issn>1076-6294</issn><issn>1931-8448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqFkUtuFDEQhi0EIiGwZBt5ySI9lN3uh5eTTgKR8hIK61a1u0Y4mrYntgeJY3CVHCRniocOETs2Lqvq86eSf8Y-ClgIaPXnaQwLCaJZgJLNK7YvdCmKVqn2db5DUxe11GqPvYvxDgAqUZdv2Z7UtVayrvfZ785PGww2esf9incYBuRXN8WJDWTS0dzYkKOJnzs0yf7EZDN7SemHH484upE_PhTd8tvxkt9STJGvfOAnlPJz-4_0jwMj8Zvgx-08s46fukTBD9lMwaIhpPfszQrXkT481wP2_ez0tvtaXFx_Oe-WF4UpyyYVupEahZHQGmlA50MNrSmhqYZmNEq0FWiQSEY0pq5aqFCMWgwGBgBErcsD9mn2boK_3-bN-8lGQ-s1OvLb2EsohRKq1XVGixk1wccYaNVvgp0w_OoF9LsU-pxCv0uh36WQ-cNn9XaYaHyh_357BsoZ2LXRubWlgUL6j_YJznaVOw</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Bayraktar, Banu</creator><creator>Barış, Ayşe</creator><creator>Malkoçoğlu, Gülşah</creator><creator>Erdemir, Duygu</creator><creator>Kına, Nur</creator><general>Mary Ann Liebert, Inc., publishers</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20190101</creationdate><title>Comparison of Carba NP-Direct, Carbapenem Inactivation Method, and β-CARBA Tests for Detection of Carbapenemase Production in Enterobacteriaceae</title><author>Bayraktar, Banu ; Barış, Ayşe ; Malkoçoğlu, Gülşah ; Erdemir, Duygu ; Kına, Nur</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-9729a1c208c2c09c2c4b8c3075b7dc41850902aec17c65805a1d91bc0b00aa993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bayraktar, Banu</creatorcontrib><creatorcontrib>Barış, Ayşe</creatorcontrib><creatorcontrib>Malkoçoğlu, Gülşah</creatorcontrib><creatorcontrib>Erdemir, Duygu</creatorcontrib><creatorcontrib>Kına, Nur</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial drug resistance (Larchmont, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bayraktar, Banu</au><au>Barış, Ayşe</au><au>Malkoçoğlu, Gülşah</au><au>Erdemir, Duygu</au><au>Kına, Nur</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of Carba NP-Direct, Carbapenem Inactivation Method, and β-CARBA Tests for Detection of Carbapenemase Production in Enterobacteriaceae</atitle><jtitle>Microbial drug resistance (Larchmont, N.Y.)</jtitle><addtitle>Microb Drug Resist</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>25</volume><issue>1</issue><spage>97</spage><epage>102</epage><pages>97-102</pages><issn>1076-6294</issn><eissn>1931-8448</eissn><abstract>Rapid and accurate detection of carbapenemase-producing isolates are extremely important for management of antimicrobial therapy and the implementation of infection control measures. We evaluated the performance of Carba NP-direct, carbapenem inactivation method (CIM), and the commercial β-CARBA tests for detection of carbapenemase production in
Enterobacteriaceae
.
Enterobacteriaceae
isolates with previously characterized carbapenemase types (
n
= 110) and non-carbapenemase-producing
Escherichia coli
(
n
= 15) isolates were tested. Sensitivities of Carba NP-direct, CIM, and β-CARBA tests were 99.0%, 92.7%, and 93.6%, respectively, while specificity was 100% for all three tests. For β-CARBA test, a 60-min incubation time instead of 30 increased the sensitivity to 98.1%, and lessened false negativity, particularly with OXA-48-like producers. Our results showed that Carba NP-direct, CIM, and β-CARBA tests are useful tools for the reliable detection of carbapenemase activity in enterobacterial isolates. Carba NP-direct is a simple, rapid, and low-cost test for routine use.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc., publishers</pub><pmid>29694266</pmid><doi>10.1089/mdr.2017.0427</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1076-6294 |
| ispartof | Microbial drug resistance (Larchmont, N.Y.), 2019-01, Vol.25 (1), p.97-102 |
| issn | 1076-6294 1931-8448 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2031414896 |
| source | Alma/SFX Local Collection |
| subjects | Epidemiology |
| title | Comparison of Carba NP-Direct, Carbapenem Inactivation Method, and β-CARBA Tests for Detection of Carbapenemase Production in Enterobacteriaceae |
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