Effect of Enzymatic Digestion of Protein Derivatives Obtained from Mucuna pruriens L. on Production of Proinflammatory Mediators by BALB/c Mouse Macrophages
Inflammation is considered to be a major risk factor for the pathogenesis of chronic non-communicable diseases. Macrophages are important immune cells, which regulate inflammation and host defense by secretion of proinflammatory mediators. Obtaining biopeptides by enzymatic hydrolysis adds value to...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2018-11, Vol.186 (3), p.597-612 |
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| creator | Martínez Leo, Edwin E. Arana Argáez, Victor E. Acevedo Fernández, Juan J. Puc, Rosa Moo Segura Campos, Maira R. |
| description | Inflammation is considered to be a major risk factor for the pathogenesis of chronic non-communicable diseases. Macrophages are important immune cells, which regulate inflammation and host defense by secretion of proinflammatory mediators. Obtaining biopeptides by enzymatic hydrolysis adds value to proteins of vegetative origin, such as
Mucuna pruriens
L. The present study evaluated the effect of enzymatic digestion of protein derivatives obtained from
M
.
pruriens
L. on the production of proinflammatory mediators by BALB/c mouse macrophages. Five different molecular weight peptide fractions were obtained (F > 10, 5–10, 3–5, 1–3, and |
| doi_str_mv | 10.1007/s12010-018-2740-4 |
| format | Article |
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Mucuna pruriens
L. The present study evaluated the effect of enzymatic digestion of protein derivatives obtained from
M
.
pruriens
L. on the production of proinflammatory mediators by BALB/c mouse macrophages. Five different molecular weight peptide fractions were obtained (F > 10, 5–10, 3–5, 1–3, and < 1 kDa, respectively). At 300 μg/mL, F5–10 kDa inhibited 50.26 and 61.00% NO and H
2
O
2
production, respectively. Moreover, F5–10 kDa reduced the IL-6 and TNFα levels to 60.25 and 69.54%, respectively. After enzymatic digestive simulation, F5–10 kDa decreased the inflammatory mediators.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-018-2740-4</identifier><identifier>PMID: 29691792</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Anti-Inflammatory Agents - pharmacology ; Biochemistry ; Biotechnology ; Cercopithecus aethiops ; Chemistry ; Chemistry and Materials Science ; Derivatives ; Digestion ; Enzymes - metabolism ; Hydrogen peroxide ; Hydrolysis ; Immune system ; Inflammation ; Inflammation Mediators - metabolism ; Interleukin 6 ; Interleukin-6 - biosynthesis ; Macrophages ; Macrophages - metabolism ; Male ; Mice, Inbred BALB C ; Molecular Weight ; Mucuna - chemistry ; Mucuna pruriens ; Pathogenesis ; Peptides - chemistry ; Peptides - metabolism ; Plant Proteins - chemistry ; Plant Proteins - metabolism ; Proteins ; Proteolysis ; Risk factors ; Rodents ; Transforming Growth Factor alpha - biosynthesis ; Tumor necrosis factor-α ; Vero Cells</subject><ispartof>Applied biochemistry and biotechnology, 2018-11, Vol.186 (3), p.597-612</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>Applied Biochemistry and Biotechnology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-91768e63d45cd1caa9e87de26bf3d54160bdb7f0b08aa6087ee31169840925b63</citedby><cites>FETCH-LOGICAL-c409t-91768e63d45cd1caa9e87de26bf3d54160bdb7f0b08aa6087ee31169840925b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-018-2740-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-018-2740-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29691792$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martínez Leo, Edwin E.</creatorcontrib><creatorcontrib>Arana Argáez, Victor E.</creatorcontrib><creatorcontrib>Acevedo Fernández, Juan J.</creatorcontrib><creatorcontrib>Puc, Rosa Moo</creatorcontrib><creatorcontrib>Segura Campos, Maira R.</creatorcontrib><title>Effect of Enzymatic Digestion of Protein Derivatives Obtained from Mucuna pruriens L. on Production of Proinflammatory Mediators by BALB/c Mouse Macrophages</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Inflammation is considered to be a major risk factor for the pathogenesis of chronic non-communicable diseases. Macrophages are important immune cells, which regulate inflammation and host defense by secretion of proinflammatory mediators. Obtaining biopeptides by enzymatic hydrolysis adds value to proteins of vegetative origin, such as
Mucuna pruriens
L. The present study evaluated the effect of enzymatic digestion of protein derivatives obtained from
M
.
pruriens
L. on the production of proinflammatory mediators by BALB/c mouse macrophages. Five different molecular weight peptide fractions were obtained (F > 10, 5–10, 3–5, 1–3, and < 1 kDa, respectively). At 300 μg/mL, F5–10 kDa inhibited 50.26 and 61.00% NO and H
2
O
2
production, respectively. Moreover, F5–10 kDa reduced the IL-6 and TNFα levels to 60.25 and 69.54%, respectively. After enzymatic digestive simulation, F5–10 kDa decreased the inflammatory mediators.</description><subject>Animals</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Biochemistry</subject><subject>Biotechnology</subject><subject>Cercopithecus aethiops</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Derivatives</subject><subject>Digestion</subject><subject>Enzymes - metabolism</subject><subject>Hydrogen peroxide</subject><subject>Hydrolysis</subject><subject>Immune system</subject><subject>Inflammation</subject><subject>Inflammation Mediators - metabolism</subject><subject>Interleukin 6</subject><subject>Interleukin-6 - biosynthesis</subject><subject>Macrophages</subject><subject>Macrophages - metabolism</subject><subject>Male</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular Weight</subject><subject>Mucuna - chemistry</subject><subject>Mucuna pruriens</subject><subject>Pathogenesis</subject><subject>Peptides - chemistry</subject><subject>Peptides - metabolism</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - metabolism</subject><subject>Proteins</subject><subject>Proteolysis</subject><subject>Risk factors</subject><subject>Rodents</subject><subject>Transforming Growth Factor alpha - biosynthesis</subject><subject>Tumor necrosis factor-α</subject><subject>Vero Cells</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kctuFDEQRS0EIkPgA9ggS2zYdFJ2P9xe5jE8pBklC1hbbrscHE3bg90dafiWfGw8THgIKSuXXOfeqtIl5C2DEwYgTjPjwKAC1ldcNFA1z8iCta2sgEv2nCyAi7rivJdH5FXOtwCM9614SY647CQTki_I_dI5NBONji7Dz92oJ2_opb_BPPkY9t_XKU7oA73E5O9K-w4zvRom7QNa6lIc6Xo2c9B0m-bkMWS6OqFFWnR2Nv-4-OA2eiwTYtrRNVq_rzIddvT8bHV-aug6zhnpWpsUt991WeE1eeH0JuObx_eYfPu4_HrxuVpdffpycbaqTANyqsopXY9dbZvWWGa0ltgLi7wbXG3bhnUw2EE4GKDXuoNeINaMdbIvat4OXX1MPhx8tyn-mMvpavTZ4GajA5adFIcaZN3IWhT0_X_obZxTKNv9oqBhXLBCsQNVTsk5oVPb5EeddoqB2kenDtGpEp3aR6eaonn36DwPI9o_it9ZFYAfgFxa4QbT39FPuz4AMTmkug</recordid><startdate>20181101</startdate><enddate>20181101</enddate><creator>Martínez Leo, Edwin E.</creator><creator>Arana Argáez, Victor E.</creator><creator>Acevedo Fernández, Juan J.</creator><creator>Puc, Rosa Moo</creator><creator>Segura Campos, Maira R.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>20181101</creationdate><title>Effect of Enzymatic Digestion of Protein Derivatives Obtained from Mucuna pruriens L. on Production of Proinflammatory Mediators by BALB/c Mouse Macrophages</title><author>Martínez Leo, Edwin E. ; Arana Argáez, Victor E. ; Acevedo Fernández, Juan J. ; Puc, Rosa Moo ; Segura Campos, Maira R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-91768e63d45cd1caa9e87de26bf3d54160bdb7f0b08aa6087ee31169840925b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Anti-Inflammatory Agents - 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Academic</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martínez Leo, Edwin E.</au><au>Arana Argáez, Victor E.</au><au>Acevedo Fernández, Juan J.</au><au>Puc, Rosa Moo</au><au>Segura Campos, Maira R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Enzymatic Digestion of Protein Derivatives Obtained from Mucuna pruriens L. on Production of Proinflammatory Mediators by BALB/c Mouse Macrophages</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2018-11-01</date><risdate>2018</risdate><volume>186</volume><issue>3</issue><spage>597</spage><epage>612</epage><pages>597-612</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>Inflammation is considered to be a major risk factor for the pathogenesis of chronic non-communicable diseases. Macrophages are important immune cells, which regulate inflammation and host defense by secretion of proinflammatory mediators. Obtaining biopeptides by enzymatic hydrolysis adds value to proteins of vegetative origin, such as
Mucuna pruriens
L. The present study evaluated the effect of enzymatic digestion of protein derivatives obtained from
M
.
pruriens
L. on the production of proinflammatory mediators by BALB/c mouse macrophages. Five different molecular weight peptide fractions were obtained (F > 10, 5–10, 3–5, 1–3, and < 1 kDa, respectively). At 300 μg/mL, F5–10 kDa inhibited 50.26 and 61.00% NO and H
2
O
2
production, respectively. Moreover, F5–10 kDa reduced the IL-6 and TNFα levels to 60.25 and 69.54%, respectively. After enzymatic digestive simulation, F5–10 kDa decreased the inflammatory mediators.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>29691792</pmid><doi>10.1007/s12010-018-2740-4</doi><tpages>16</tpages></addata></record> |
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| ispartof | Applied biochemistry and biotechnology, 2018-11, Vol.186 (3), p.597-612 |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Anti-Inflammatory Agents - pharmacology Biochemistry Biotechnology Cercopithecus aethiops Chemistry Chemistry and Materials Science Derivatives Digestion Enzymes - metabolism Hydrogen peroxide Hydrolysis Immune system Inflammation Inflammation Mediators - metabolism Interleukin 6 Interleukin-6 - biosynthesis Macrophages Macrophages - metabolism Male Mice, Inbred BALB C Molecular Weight Mucuna - chemistry Mucuna pruriens Pathogenesis Peptides - chemistry Peptides - metabolism Plant Proteins - chemistry Plant Proteins - metabolism Proteins Proteolysis Risk factors Rodents Transforming Growth Factor alpha - biosynthesis Tumor necrosis factor-α Vero Cells |
| title | Effect of Enzymatic Digestion of Protein Derivatives Obtained from Mucuna pruriens L. on Production of Proinflammatory Mediators by BALB/c Mouse Macrophages |
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