C-Phycocyanin inhibits hepatic gluconeogenesis and increases glycogen synthesis via activating Akt and AMPK in insulin resistance hepatocytes
C-Phycocyanin (C-PC), a kind of blue protein isolated from Spirulina platensis, can ameliorate hyperglycemia, but its effects on gluconeogenesis and glycogenesis are unknown. In the present study, we investigated the effects and underlying mechanisms of C-PC on gluconeogenesis and glycogenesis in in...
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| Veröffentlicht in: | Food & function 2018-05, Vol.9 (5), p.2829-2839 |
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| creator | Ren, Zhiheng Xie, Zhifei Cao, Danni Gong, Mufeng Yang, Lei Zhou, Zhu Ou, Yu |
| description | C-Phycocyanin (C-PC), a kind of blue protein isolated from Spirulina platensis, can ameliorate hyperglycemia, but its effects on gluconeogenesis and glycogenesis are unknown. In the present study, we investigated the effects and underlying mechanisms of C-PC on gluconeogenesis and glycogenesis in insulin resistant hepatocytes. Insulin resistance was induced by high glucose (HG) in human hepatocellular carcinoma (HepG2) cells. C-PC ameliorated glucose production and phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) expression in HG-induced insulin resistant HepG2 cells. It also increased glucose uptake, glycogen content and glycogen synthase (GS) activation in HG-induced insulin resistant HepG2 cells. The data revealed the mechanism of C-PC in improving glucose homoeostasis via activating the IRS/PI3 K/Akt and SIRT1/LKB1/AMPK signaling pathway in insulin resistant hepatocytes. C-PC could be a promising leading compound for the development of a hypoglycemic agent. |
| doi_str_mv | 10.1039/c8fo00257f |
| format | Article |
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In the present study, we investigated the effects and underlying mechanisms of C-PC on gluconeogenesis and glycogenesis in insulin resistant hepatocytes. Insulin resistance was induced by high glucose (HG) in human hepatocellular carcinoma (HepG2) cells. C-PC ameliorated glucose production and phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) expression in HG-induced insulin resistant HepG2 cells. It also increased glucose uptake, glycogen content and glycogen synthase (GS) activation in HG-induced insulin resistant HepG2 cells. The data revealed the mechanism of C-PC in improving glucose homoeostasis via activating the IRS/PI3 K/Akt and SIRT1/LKB1/AMPK signaling pathway in insulin resistant hepatocytes. C-PC could be a promising leading compound for the development of a hypoglycemic agent.</description><identifier>ISSN: 2042-6496</identifier><identifier>EISSN: 2042-650X</identifier><identifier>DOI: 10.1039/c8fo00257f</identifier><identifier>PMID: 29693104</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>AKT protein ; AMP-Activated Protein Kinases - genetics ; AMP-Activated Protein Kinases - metabolism ; Down-Regulation - drug effects ; Gluconeogenesis ; Gluconeogenesis - drug effects ; Glucose ; Glucose - biosynthesis ; Glucose-6-phosphatase ; Glycogen ; Glycogen - biosynthesis ; Glycogen synthase ; Hep G2 Cells ; Hepatocellular carcinoma ; Hepatocytes ; Hepatocytes - drug effects ; Hepatocytes - metabolism ; Humans ; Hyperglycemia ; Insulin ; Insulin - metabolism ; Insulin Resistance ; Liver - drug effects ; Liver - metabolism ; LKB1 protein ; Phycocyanin ; Phycocyanin - pharmacology ; Plant Extracts - pharmacology ; Proteins ; Proto-Oncogene Proteins c-akt - genetics ; Proto-Oncogene Proteins c-akt - metabolism ; Signal transduction ; SIRT1 protein ; Spirulina - chemistry</subject><ispartof>Food & function, 2018-05, Vol.9 (5), p.2829-2839</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-3e850733c5c626a7ab15c3896b0b53013cd67d7269851fd680dbcb1e81acbaa03</citedby><cites>FETCH-LOGICAL-c356t-3e850733c5c626a7ab15c3896b0b53013cd67d7269851fd680dbcb1e81acbaa03</cites><orcidid>0000-0002-1794-7861</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29693104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ren, Zhiheng</creatorcontrib><creatorcontrib>Xie, Zhifei</creatorcontrib><creatorcontrib>Cao, Danni</creatorcontrib><creatorcontrib>Gong, Mufeng</creatorcontrib><creatorcontrib>Yang, Lei</creatorcontrib><creatorcontrib>Zhou, Zhu</creatorcontrib><creatorcontrib>Ou, Yu</creatorcontrib><title>C-Phycocyanin inhibits hepatic gluconeogenesis and increases glycogen synthesis via activating Akt and AMPK in insulin resistance hepatocytes</title><title>Food & function</title><addtitle>Food Funct</addtitle><description>C-Phycocyanin (C-PC), a kind of blue protein isolated from Spirulina platensis, can ameliorate hyperglycemia, but its effects on gluconeogenesis and glycogenesis are unknown. 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C-PC could be a promising leading compound for the development of a hypoglycemic agent.</description><subject>AKT protein</subject><subject>AMP-Activated Protein Kinases - genetics</subject><subject>AMP-Activated Protein Kinases - metabolism</subject><subject>Down-Regulation - drug effects</subject><subject>Gluconeogenesis</subject><subject>Gluconeogenesis - drug effects</subject><subject>Glucose</subject><subject>Glucose - biosynthesis</subject><subject>Glucose-6-phosphatase</subject><subject>Glycogen</subject><subject>Glycogen - biosynthesis</subject><subject>Glycogen synthase</subject><subject>Hep G2 Cells</subject><subject>Hepatocellular carcinoma</subject><subject>Hepatocytes</subject><subject>Hepatocytes - drug effects</subject><subject>Hepatocytes - metabolism</subject><subject>Humans</subject><subject>Hyperglycemia</subject><subject>Insulin</subject><subject>Insulin - metabolism</subject><subject>Insulin Resistance</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>LKB1 protein</subject><subject>Phycocyanin</subject><subject>Phycocyanin - pharmacology</subject><subject>Plant Extracts - pharmacology</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins c-akt - genetics</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>Signal transduction</subject><subject>SIRT1 protein</subject><subject>Spirulina - chemistry</subject><issn>2042-6496</issn><issn>2042-650X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkcFKAzEQhoMoKurFB5AFLyKsTpJuNjmWYlVU2oOCtyU7m22j22zdZIU-hO9s2qoHc5nAfP_8M_yEnFK4osDVNcq6BWBZXu-QQwYDlooMXnd__wMlDsiJ928QH1dKKrlPDpgSilMYHJKvUTqdr7DFlXbWJdbNbWmDT-ZmqYPFZNb02DrTzowz3vpEuypC2BntjY_dKI2txK9cmG-AT6sTjcF-RrmbJcP3sNEMn6YPycbA902s3RoO2qHZWsUFgvHHZK_WjTcnP_WIvIxvnkd36ePk9n40fEyRZyKk3MgMcs4xQ8GEznVJM-RSiRLKjAPlWIm8yplQMqN1JSRUJZbUSKqx1Br4EbnYzl127UdvfCgW1qNpGh1P7X3BgINiTNE1ev4PfWv7zsXtIjXgwNYukbrcUti13nemLpadXehuVVAo1jkVIzmebHIaR_jsZ2RfLkz1h_6mwr8BuXuPbw</recordid><startdate>20180523</startdate><enddate>20180523</enddate><creator>Ren, Zhiheng</creator><creator>Xie, Zhifei</creator><creator>Cao, Danni</creator><creator>Gong, Mufeng</creator><creator>Yang, Lei</creator><creator>Zhou, Zhu</creator><creator>Ou, Yu</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1794-7861</orcidid></search><sort><creationdate>20180523</creationdate><title>C-Phycocyanin inhibits hepatic gluconeogenesis and increases glycogen synthesis via activating Akt and AMPK in insulin resistance hepatocytes</title><author>Ren, Zhiheng ; Xie, Zhifei ; Cao, Danni ; Gong, Mufeng ; Yang, Lei ; Zhou, Zhu ; Ou, Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-3e850733c5c626a7ab15c3896b0b53013cd67d7269851fd680dbcb1e81acbaa03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>AKT protein</topic><topic>AMP-Activated Protein Kinases - genetics</topic><topic>AMP-Activated Protein Kinases - metabolism</topic><topic>Down-Regulation - drug effects</topic><topic>Gluconeogenesis</topic><topic>Gluconeogenesis - drug effects</topic><topic>Glucose</topic><topic>Glucose - biosynthesis</topic><topic>Glucose-6-phosphatase</topic><topic>Glycogen</topic><topic>Glycogen - biosynthesis</topic><topic>Glycogen synthase</topic><topic>Hep G2 Cells</topic><topic>Hepatocellular carcinoma</topic><topic>Hepatocytes</topic><topic>Hepatocytes - drug effects</topic><topic>Hepatocytes - metabolism</topic><topic>Humans</topic><topic>Hyperglycemia</topic><topic>Insulin</topic><topic>Insulin - metabolism</topic><topic>Insulin Resistance</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>LKB1 protein</topic><topic>Phycocyanin</topic><topic>Phycocyanin - pharmacology</topic><topic>Plant Extracts - pharmacology</topic><topic>Proteins</topic><topic>Proto-Oncogene Proteins c-akt - genetics</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>Signal transduction</topic><topic>SIRT1 protein</topic><topic>Spirulina - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ren, Zhiheng</creatorcontrib><creatorcontrib>Xie, Zhifei</creatorcontrib><creatorcontrib>Cao, Danni</creatorcontrib><creatorcontrib>Gong, Mufeng</creatorcontrib><creatorcontrib>Yang, Lei</creatorcontrib><creatorcontrib>Zhou, Zhu</creatorcontrib><creatorcontrib>Ou, Yu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Food & function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ren, Zhiheng</au><au>Xie, Zhifei</au><au>Cao, Danni</au><au>Gong, Mufeng</au><au>Yang, Lei</au><au>Zhou, Zhu</au><au>Ou, Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>C-Phycocyanin inhibits hepatic gluconeogenesis and increases glycogen synthesis via activating Akt and AMPK in insulin resistance hepatocytes</atitle><jtitle>Food & function</jtitle><addtitle>Food Funct</addtitle><date>2018-05-23</date><risdate>2018</risdate><volume>9</volume><issue>5</issue><spage>2829</spage><epage>2839</epage><pages>2829-2839</pages><issn>2042-6496</issn><eissn>2042-650X</eissn><abstract>C-Phycocyanin (C-PC), a kind of blue protein isolated from Spirulina platensis, can ameliorate hyperglycemia, but its effects on gluconeogenesis and glycogenesis are unknown. In the present study, we investigated the effects and underlying mechanisms of C-PC on gluconeogenesis and glycogenesis in insulin resistant hepatocytes. Insulin resistance was induced by high glucose (HG) in human hepatocellular carcinoma (HepG2) cells. C-PC ameliorated glucose production and phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) expression in HG-induced insulin resistant HepG2 cells. It also increased glucose uptake, glycogen content and glycogen synthase (GS) activation in HG-induced insulin resistant HepG2 cells. The data revealed the mechanism of C-PC in improving glucose homoeostasis via activating the IRS/PI3 K/Akt and SIRT1/LKB1/AMPK signaling pathway in insulin resistant hepatocytes. C-PC could be a promising leading compound for the development of a hypoglycemic agent.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>29693104</pmid><doi>10.1039/c8fo00257f</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-1794-7861</orcidid></addata></record> |
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| subjects | AKT protein AMP-Activated Protein Kinases - genetics AMP-Activated Protein Kinases - metabolism Down-Regulation - drug effects Gluconeogenesis Gluconeogenesis - drug effects Glucose Glucose - biosynthesis Glucose-6-phosphatase Glycogen Glycogen - biosynthesis Glycogen synthase Hep G2 Cells Hepatocellular carcinoma Hepatocytes Hepatocytes - drug effects Hepatocytes - metabolism Humans Hyperglycemia Insulin Insulin - metabolism Insulin Resistance Liver - drug effects Liver - metabolism LKB1 protein Phycocyanin Phycocyanin - pharmacology Plant Extracts - pharmacology Proteins Proto-Oncogene Proteins c-akt - genetics Proto-Oncogene Proteins c-akt - metabolism Signal transduction SIRT1 protein Spirulina - chemistry |
| title | C-Phycocyanin inhibits hepatic gluconeogenesis and increases glycogen synthesis via activating Akt and AMPK in insulin resistance hepatocytes |
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