Cell-cycle arrest and apoptosis induction in human breast carcinoma MCF-7 cells by carboxymethylated β-glucan from the mushroom sclerotia of Pleurotus tuber-regium
The mechanism for the anti-tumor activity of a water-soluble carboxymethylated β-glucan (CMPTR), partially synthesized from an insoluble native glucan isolated from the sclerotia of Pleurotus tuber-regium, was studied using human breast carcinoma MCF-7 breast cancer cells in vitro. CMPTR-induced ant...
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| Veröffentlicht in: | Carbohydrate polymers 2006-11, Vol.66 (4), p.455-462 |
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| creator | Zhang, Mei Cheung, Peter C.-K. Chiu, Lawrence C.-M. Wong, Elaine Y.-L. Ooi, Vincent E.-C. |
| description | The mechanism for the anti-tumor activity of a water-soluble carboxymethylated β-glucan (CMPTR), partially synthesized from an insoluble native glucan isolated from the sclerotia of
Pleurotus tuber-regium, was studied using human breast carcinoma MCF-7 breast cancer cells
in vitro. CMPTR-induced anti-proliferative activity dose-dependently, with an IC
50 of 204
μg/ml. CMPTR inhibited the cell proliferation of MCF-7 by arresting the G
1 phase of its cell cycle after 48
h of incubation as shown by flow cytometry. Such G
1 phase arrest was associated with the down-regulation of cyclin D1 and cyclin E expressions in the breast cancer cells. In addition, the CMPTR-treated MCF-7 cancer cells were associated with decreased expression of anti-apoptotic Bcl-2 protein and increased expression of Bax/Bcl-2 ratio. This study shows that CMPTR can inhibit the proliferation of MCF-7 by cell-cycle arrest and apoptosis induction. The potential development of this mushroom polysaccharide as a water-soluble anti-tumor agent requires further investigation. |
| doi_str_mv | 10.1016/j.carbpol.2006.03.031 |
| format | Article |
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Pleurotus tuber-regium, was studied using human breast carcinoma MCF-7 breast cancer cells
in vitro. CMPTR-induced anti-proliferative activity dose-dependently, with an IC
50 of 204
μg/ml. CMPTR inhibited the cell proliferation of MCF-7 by arresting the G
1 phase of its cell cycle after 48
h of incubation as shown by flow cytometry. Such G
1 phase arrest was associated with the down-regulation of cyclin D1 and cyclin E expressions in the breast cancer cells. In addition, the CMPTR-treated MCF-7 cancer cells were associated with decreased expression of anti-apoptotic Bcl-2 protein and increased expression of Bax/Bcl-2 ratio. This study shows that CMPTR can inhibit the proliferation of MCF-7 by cell-cycle arrest and apoptosis induction. The potential development of this mushroom polysaccharide as a water-soluble anti-tumor agent requires further investigation.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2006.03.031</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Antineoplastic agents ; Apoptosis ; Applied sciences ; Biological and medical sciences ; Carboxymethylation ; Cell-cycle arrest ; Exact sciences and technology ; General aspects ; MCF-7 cells ; Medical sciences ; Mushroom sclerotia ; Natural polymers ; Pharmacology. Drug treatments ; Physicochemistry of polymers ; Pleurotus tuber-regium ; Sclerotia ; Starch and polysaccharides ; β-Glucan</subject><ispartof>Carbohydrate polymers, 2006-11, Vol.66 (4), p.455-462</ispartof><rights>2006 Elsevier Ltd</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-cb306f9c0d2be23a1d1b96666f95da3c9d148a0426bf4ed22d0fc3ea85ba94d53</citedby><cites>FETCH-LOGICAL-c370t-cb306f9c0d2be23a1d1b96666f95da3c9d148a0426bf4ed22d0fc3ea85ba94d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.carbpol.2006.03.031$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18284628$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Mei</creatorcontrib><creatorcontrib>Cheung, Peter C.-K.</creatorcontrib><creatorcontrib>Chiu, Lawrence C.-M.</creatorcontrib><creatorcontrib>Wong, Elaine Y.-L.</creatorcontrib><creatorcontrib>Ooi, Vincent E.-C.</creatorcontrib><title>Cell-cycle arrest and apoptosis induction in human breast carcinoma MCF-7 cells by carboxymethylated β-glucan from the mushroom sclerotia of Pleurotus tuber-regium</title><title>Carbohydrate polymers</title><description>The mechanism for the anti-tumor activity of a water-soluble carboxymethylated β-glucan (CMPTR), partially synthesized from an insoluble native glucan isolated from the sclerotia of
Pleurotus tuber-regium, was studied using human breast carcinoma MCF-7 breast cancer cells
in vitro. CMPTR-induced anti-proliferative activity dose-dependently, with an IC
50 of 204
μg/ml. CMPTR inhibited the cell proliferation of MCF-7 by arresting the G
1 phase of its cell cycle after 48
h of incubation as shown by flow cytometry. Such G
1 phase arrest was associated with the down-regulation of cyclin D1 and cyclin E expressions in the breast cancer cells. In addition, the CMPTR-treated MCF-7 cancer cells were associated with decreased expression of anti-apoptotic Bcl-2 protein and increased expression of Bax/Bcl-2 ratio. This study shows that CMPTR can inhibit the proliferation of MCF-7 by cell-cycle arrest and apoptosis induction. The potential development of this mushroom polysaccharide as a water-soluble anti-tumor agent requires further investigation.</description><subject>Antineoplastic agents</subject><subject>Apoptosis</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Carboxymethylation</subject><subject>Cell-cycle arrest</subject><subject>Exact sciences and technology</subject><subject>General aspects</subject><subject>MCF-7 cells</subject><subject>Medical sciences</subject><subject>Mushroom sclerotia</subject><subject>Natural polymers</subject><subject>Pharmacology. Drug treatments</subject><subject>Physicochemistry of polymers</subject><subject>Pleurotus tuber-regium</subject><subject>Sclerotia</subject><subject>Starch and polysaccharides</subject><subject>β-Glucan</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFUcuqFDEQbUTB8eonCNnorse8-rUSGbwqXNGFrkMlqb6TobvT5iG3_8cv8EP8JjPMgEuLgkpR59ShcqrqJaN7Rln75rQ3EPTqpz2ntN1TUZI9qnas74aaCSkfVzvKpKz7lnVPq2cxnmiJltFd9euA01SbzUxIIASMicBiCax-TT66SNxis0nOL-VFjnmGheiAUHBF1LjFz0A-H27rjpiyKRK9nQfaP2wzpuM2QUJL_vyu76dsCncMfibpiGTO8Rh8aWKRDj45IH4kXyfMpcmRpKwx1AHvXZ6fV09GmCK-uNab6vvt-2-Hj_Xdlw-fDu_uaiM6mmqjBW3HwVDLNXIBzDI9tCXGobEgzGCZ7IFK3upRouXc0tEIhL7RMEjbiJvq9WXvGvyPXP5CzS6ez4IFfY6KU0Eb0XcF2FyAJvgYA45qDW6GsClG1dkTdVJXT9TZE0VFSVZ4r64CEA1MY4DFuPiP3PNetrwvuLcXHJZrfzoMKhqHi0HrApqkrHf_UfoLDoWqGg</recordid><startdate>20061123</startdate><enddate>20061123</enddate><creator>Zhang, Mei</creator><creator>Cheung, Peter C.-K.</creator><creator>Chiu, Lawrence C.-M.</creator><creator>Wong, Elaine Y.-L.</creator><creator>Ooi, Vincent E.-C.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20061123</creationdate><title>Cell-cycle arrest and apoptosis induction in human breast carcinoma MCF-7 cells by carboxymethylated β-glucan from the mushroom sclerotia of Pleurotus tuber-regium</title><author>Zhang, Mei ; Cheung, Peter C.-K. ; Chiu, Lawrence C.-M. ; Wong, Elaine Y.-L. ; Ooi, Vincent E.-C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-cb306f9c0d2be23a1d1b96666f95da3c9d148a0426bf4ed22d0fc3ea85ba94d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Antineoplastic agents</topic><topic>Apoptosis</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Carboxymethylation</topic><topic>Cell-cycle arrest</topic><topic>Exact sciences and technology</topic><topic>General aspects</topic><topic>MCF-7 cells</topic><topic>Medical sciences</topic><topic>Mushroom sclerotia</topic><topic>Natural polymers</topic><topic>Pharmacology. Drug treatments</topic><topic>Physicochemistry of polymers</topic><topic>Pleurotus tuber-regium</topic><topic>Sclerotia</topic><topic>Starch and polysaccharides</topic><topic>β-Glucan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Mei</creatorcontrib><creatorcontrib>Cheung, Peter C.-K.</creatorcontrib><creatorcontrib>Chiu, Lawrence C.-M.</creatorcontrib><creatorcontrib>Wong, Elaine Y.-L.</creatorcontrib><creatorcontrib>Ooi, Vincent E.-C.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Mei</au><au>Cheung, Peter C.-K.</au><au>Chiu, Lawrence C.-M.</au><au>Wong, Elaine Y.-L.</au><au>Ooi, Vincent E.-C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell-cycle arrest and apoptosis induction in human breast carcinoma MCF-7 cells by carboxymethylated β-glucan from the mushroom sclerotia of Pleurotus tuber-regium</atitle><jtitle>Carbohydrate polymers</jtitle><date>2006-11-23</date><risdate>2006</risdate><volume>66</volume><issue>4</issue><spage>455</spage><epage>462</epage><pages>455-462</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>The mechanism for the anti-tumor activity of a water-soluble carboxymethylated β-glucan (CMPTR), partially synthesized from an insoluble native glucan isolated from the sclerotia of
Pleurotus tuber-regium, was studied using human breast carcinoma MCF-7 breast cancer cells
in vitro. CMPTR-induced anti-proliferative activity dose-dependently, with an IC
50 of 204
μg/ml. CMPTR inhibited the cell proliferation of MCF-7 by arresting the G
1 phase of its cell cycle after 48
h of incubation as shown by flow cytometry. Such G
1 phase arrest was associated with the down-regulation of cyclin D1 and cyclin E expressions in the breast cancer cells. In addition, the CMPTR-treated MCF-7 cancer cells were associated with decreased expression of anti-apoptotic Bcl-2 protein and increased expression of Bax/Bcl-2 ratio. This study shows that CMPTR can inhibit the proliferation of MCF-7 by cell-cycle arrest and apoptosis induction. The potential development of this mushroom polysaccharide as a water-soluble anti-tumor agent requires further investigation.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.carbpol.2006.03.031</doi><tpages>8</tpages></addata></record> |
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| ispartof | Carbohydrate polymers, 2006-11, Vol.66 (4), p.455-462 |
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| subjects | Antineoplastic agents Apoptosis Applied sciences Biological and medical sciences Carboxymethylation Cell-cycle arrest Exact sciences and technology General aspects MCF-7 cells Medical sciences Mushroom sclerotia Natural polymers Pharmacology. Drug treatments Physicochemistry of polymers Pleurotus tuber-regium Sclerotia Starch and polysaccharides β-Glucan |
| title | Cell-cycle arrest and apoptosis induction in human breast carcinoma MCF-7 cells by carboxymethylated β-glucan from the mushroom sclerotia of Pleurotus tuber-regium |
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