Effect evaluation of methylprednisolone plus mitochondrial division inhibitor-1 on spinal cord injury rats
Purpose To investigate the combination effect of methylprednisolone (MP) and mitochondrial division inhibitor-1 (Mdivi-1) on the neurological function recovery of rat spinal cord injury (SCI) model. Methods The weight-drop method was used to establish the rat SCI model; then, rats were randomized in...
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| Veröffentlicht in: | Child's nervous system 2018-08, Vol.34 (8), p.1479-1487 |
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| creator | Chen, Xu-Gui Chen, Li-Hua Xu, Ru-Xiang Zhang, Hong-Tian |
| description | Purpose
To investigate the combination effect of methylprednisolone (MP) and mitochondrial division inhibitor-1 (Mdivi-1) on the neurological function recovery of rat spinal cord injury (SCI) model.
Methods
The weight-drop method was used to establish the rat SCI model; then, rats were randomized into sham group, SCI group, MP group, Mdivi-1 group and MP+Mdivi-1 group. Motor function scores were quantified to evaluate locomotor ability; HE staining was used to assess spinal cord histopathology; tissue water content, oxidative stress, tissue mitochondrial function, neurons apoptosis, and apoptosis-related protein expression were detected.
Results
From the third day after SCI, BBB score of the MP+Mdivi-1 group was obviously higher than the other experimental groups (
p
|
| doi_str_mv | 10.1007/s00381-018-3792-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2029640055</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2029640055</sourcerecordid><originalsourceid>FETCH-LOGICAL-c344t-c49686bcb2439ba028b2a64470bd6aa629e54ae3faeb05923ab185bc1c032dc03</originalsourceid><addsrcrecordid>eNp9kLtOwzAUhi0EoqXwACwoI4vh-JI0GVFVLlIlFpgt23GooyQOdlKpfXpcpTCyHOvov-j4Q-iWwAMBWD4GAJYTDCTHbFlQfDhDc8IZw8BSOEdzoGmGl8Bhhq5CqAFImtPiEs1okeU0y4s5qtdVZfSQmJ1sRjlY1yWuSlozbPdN703Z2eAa15mkb8aQtHZweuu60lvZJKXd2XBM2G5rVZQ8JklcQ2-7KGvnyyjVo98nXg7hGl1Usgnm5vQu0Ofz-mP1ijfvL2-rpw3WjPMBax6Py5RWlLNCSaC5ojLjfAmqzKTMaGFSLg2rpFGQFpRJRfJUaaKB0TKOBbqfenvvvkcTBtHaoE3TyM64MQgK8fscIE2jlUxW7V0I3lSi97aVfi8IiCNiMSEWEbE4IhaHmLk71Y-qNeVf4pdpNNDJEKLUfRkvajf6SCT80_oDuvqJwA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2029640055</pqid></control><display><type>article</type><title>Effect evaluation of methylprednisolone plus mitochondrial division inhibitor-1 on spinal cord injury rats</title><source>SpringerLink Journals</source><creator>Chen, Xu-Gui ; Chen, Li-Hua ; Xu, Ru-Xiang ; Zhang, Hong-Tian</creator><creatorcontrib>Chen, Xu-Gui ; Chen, Li-Hua ; Xu, Ru-Xiang ; Zhang, Hong-Tian</creatorcontrib><description>Purpose
To investigate the combination effect of methylprednisolone (MP) and mitochondrial division inhibitor-1 (Mdivi-1) on the neurological function recovery of rat spinal cord injury (SCI) model.
Methods
The weight-drop method was used to establish the rat SCI model; then, rats were randomized into sham group, SCI group, MP group, Mdivi-1 group and MP+Mdivi-1 group. Motor function scores were quantified to evaluate locomotor ability; HE staining was used to assess spinal cord histopathology; tissue water content, oxidative stress, tissue mitochondrial function, neurons apoptosis, and apoptosis-related protein expression were detected.
Results
From the third day after SCI, BBB score of the MP+Mdivi-1 group was obviously higher than the other experimental groups (
p
< 0.05). Compared with the SCI group, tissue water content of the Mdivi-1 group and MP+Mdivi-1 group reduced obviously (
p
< 0.05), mitochondrial membrane potential (MMP) level and ATP content in the Mdivi-1 group and MP+Mdivi-1 group were both higher (
p
< 0.05). Meanwhile, three kinds of treatment all reduced apoptosis significantly, while MP plus Mdivi-1 exhibited the best inhibition effect on apoptosis (
p
< 0.05). The expression levels of Drp1, cytochrome c, and caspase-3 were all upregulated obviously; Mdivi-1 could inhibit Drp1 upregulation induced by SCI; for the upregulation of cytochrome c and caspase-3, the inhibition effect of Mdivi-1 approached MP. When MP combined with Mdivi-1, there was the best inhibition effect.
Conclusions
MP combined with Mdivi-1 may produce better neurological function recovery, through improving functional status of mitochondria and inhibiting lipid peroxidation in damaged tissue of SCI rats, and thus alleviating apoptosis.</description><identifier>ISSN: 0256-7040</identifier><identifier>EISSN: 1433-0350</identifier><identifier>DOI: 10.1007/s00381-018-3792-z</identifier><identifier>PMID: 29682689</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Medicine ; Medicine & Public Health ; Neurosciences ; Neurosurgery ; Original Paper</subject><ispartof>Child's nervous system, 2018-08, Vol.34 (8), p.1479-1487</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-c49686bcb2439ba028b2a64470bd6aa629e54ae3faeb05923ab185bc1c032dc03</citedby><cites>FETCH-LOGICAL-c344t-c49686bcb2439ba028b2a64470bd6aa629e54ae3faeb05923ab185bc1c032dc03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00381-018-3792-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00381-018-3792-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29682689$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Xu-Gui</creatorcontrib><creatorcontrib>Chen, Li-Hua</creatorcontrib><creatorcontrib>Xu, Ru-Xiang</creatorcontrib><creatorcontrib>Zhang, Hong-Tian</creatorcontrib><title>Effect evaluation of methylprednisolone plus mitochondrial division inhibitor-1 on spinal cord injury rats</title><title>Child's nervous system</title><addtitle>Childs Nerv Syst</addtitle><addtitle>Childs Nerv Syst</addtitle><description>Purpose
To investigate the combination effect of methylprednisolone (MP) and mitochondrial division inhibitor-1 (Mdivi-1) on the neurological function recovery of rat spinal cord injury (SCI) model.
Methods
The weight-drop method was used to establish the rat SCI model; then, rats were randomized into sham group, SCI group, MP group, Mdivi-1 group and MP+Mdivi-1 group. Motor function scores were quantified to evaluate locomotor ability; HE staining was used to assess spinal cord histopathology; tissue water content, oxidative stress, tissue mitochondrial function, neurons apoptosis, and apoptosis-related protein expression were detected.
Results
From the third day after SCI, BBB score of the MP+Mdivi-1 group was obviously higher than the other experimental groups (
p
< 0.05). Compared with the SCI group, tissue water content of the Mdivi-1 group and MP+Mdivi-1 group reduced obviously (
p
< 0.05), mitochondrial membrane potential (MMP) level and ATP content in the Mdivi-1 group and MP+Mdivi-1 group were both higher (
p
< 0.05). Meanwhile, three kinds of treatment all reduced apoptosis significantly, while MP plus Mdivi-1 exhibited the best inhibition effect on apoptosis (
p
< 0.05). The expression levels of Drp1, cytochrome c, and caspase-3 were all upregulated obviously; Mdivi-1 could inhibit Drp1 upregulation induced by SCI; for the upregulation of cytochrome c and caspase-3, the inhibition effect of Mdivi-1 approached MP. When MP combined with Mdivi-1, there was the best inhibition effect.
Conclusions
MP combined with Mdivi-1 may produce better neurological function recovery, through improving functional status of mitochondria and inhibiting lipid peroxidation in damaged tissue of SCI rats, and thus alleviating apoptosis.</description><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Neurosciences</subject><subject>Neurosurgery</subject><subject>Original Paper</subject><issn>0256-7040</issn><issn>1433-0350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kLtOwzAUhi0EoqXwACwoI4vh-JI0GVFVLlIlFpgt23GooyQOdlKpfXpcpTCyHOvov-j4Q-iWwAMBWD4GAJYTDCTHbFlQfDhDc8IZw8BSOEdzoGmGl8Bhhq5CqAFImtPiEs1okeU0y4s5qtdVZfSQmJ1sRjlY1yWuSlozbPdN703Z2eAa15mkb8aQtHZweuu60lvZJKXd2XBM2G5rVZQ8JklcQ2-7KGvnyyjVo98nXg7hGl1Usgnm5vQu0Ofz-mP1ijfvL2-rpw3WjPMBax6Py5RWlLNCSaC5ojLjfAmqzKTMaGFSLg2rpFGQFpRJRfJUaaKB0TKOBbqfenvvvkcTBtHaoE3TyM64MQgK8fscIE2jlUxW7V0I3lSi97aVfi8IiCNiMSEWEbE4IhaHmLk71Y-qNeVf4pdpNNDJEKLUfRkvajf6SCT80_oDuvqJwA</recordid><startdate>20180801</startdate><enddate>20180801</enddate><creator>Chen, Xu-Gui</creator><creator>Chen, Li-Hua</creator><creator>Xu, Ru-Xiang</creator><creator>Zhang, Hong-Tian</creator><general>Springer Berlin Heidelberg</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180801</creationdate><title>Effect evaluation of methylprednisolone plus mitochondrial division inhibitor-1 on spinal cord injury rats</title><author>Chen, Xu-Gui ; Chen, Li-Hua ; Xu, Ru-Xiang ; Zhang, Hong-Tian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-c49686bcb2439ba028b2a64470bd6aa629e54ae3faeb05923ab185bc1c032dc03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Neurosciences</topic><topic>Neurosurgery</topic><topic>Original Paper</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Xu-Gui</creatorcontrib><creatorcontrib>Chen, Li-Hua</creatorcontrib><creatorcontrib>Xu, Ru-Xiang</creatorcontrib><creatorcontrib>Zhang, Hong-Tian</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Child's nervous system</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Xu-Gui</au><au>Chen, Li-Hua</au><au>Xu, Ru-Xiang</au><au>Zhang, Hong-Tian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect evaluation of methylprednisolone plus mitochondrial division inhibitor-1 on spinal cord injury rats</atitle><jtitle>Child's nervous system</jtitle><stitle>Childs Nerv Syst</stitle><addtitle>Childs Nerv Syst</addtitle><date>2018-08-01</date><risdate>2018</risdate><volume>34</volume><issue>8</issue><spage>1479</spage><epage>1487</epage><pages>1479-1487</pages><issn>0256-7040</issn><eissn>1433-0350</eissn><abstract>Purpose
To investigate the combination effect of methylprednisolone (MP) and mitochondrial division inhibitor-1 (Mdivi-1) on the neurological function recovery of rat spinal cord injury (SCI) model.
Methods
The weight-drop method was used to establish the rat SCI model; then, rats were randomized into sham group, SCI group, MP group, Mdivi-1 group and MP+Mdivi-1 group. Motor function scores were quantified to evaluate locomotor ability; HE staining was used to assess spinal cord histopathology; tissue water content, oxidative stress, tissue mitochondrial function, neurons apoptosis, and apoptosis-related protein expression were detected.
Results
From the third day after SCI, BBB score of the MP+Mdivi-1 group was obviously higher than the other experimental groups (
p
< 0.05). Compared with the SCI group, tissue water content of the Mdivi-1 group and MP+Mdivi-1 group reduced obviously (
p
< 0.05), mitochondrial membrane potential (MMP) level and ATP content in the Mdivi-1 group and MP+Mdivi-1 group were both higher (
p
< 0.05). Meanwhile, three kinds of treatment all reduced apoptosis significantly, while MP plus Mdivi-1 exhibited the best inhibition effect on apoptosis (
p
< 0.05). The expression levels of Drp1, cytochrome c, and caspase-3 were all upregulated obviously; Mdivi-1 could inhibit Drp1 upregulation induced by SCI; for the upregulation of cytochrome c and caspase-3, the inhibition effect of Mdivi-1 approached MP. When MP combined with Mdivi-1, there was the best inhibition effect.
Conclusions
MP combined with Mdivi-1 may produce better neurological function recovery, through improving functional status of mitochondria and inhibiting lipid peroxidation in damaged tissue of SCI rats, and thus alleviating apoptosis.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29682689</pmid><doi>10.1007/s00381-018-3792-z</doi><tpages>9</tpages></addata></record> |
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| subjects | Medicine Medicine & Public Health Neurosciences Neurosurgery Original Paper |
| title | Effect evaluation of methylprednisolone plus mitochondrial division inhibitor-1 on spinal cord injury rats |
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