Influence of sampling technique and bedding type on the milk microbiota: Results of a pilot study

The objective of this pilot study was to evaluate the influence of sampling technique and exposure to different bedding types on the milk microbiome of healthy primiparous cows. Primiparous Holstein cows (n = 20) with no history of clinical mastitis or monthly somatic cell counts >150,000 cells/m...

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Veröffentlicht in:	Journal of dairy science 2018-07, Vol.101 (7), p.6346-6356
Hauptverfasser:	Metzger, S.A., Hernandez, L.L., Skarlupka, J.H., Suen, G., Walker, T.M., Ruegg, P.L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteria - isolation & purification Bedding and Linens - veterinary Cattle Cell Count dairy DNA sequencing Female Housing, Animal Mastitis, Bovine - diagnosis Milk - microbiology milk microbiome milk sampling Pilot Projects
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Beschreibung
Zusammenfassung:	The objective of this pilot study was to evaluate the influence of sampling technique and exposure to different bedding types on the milk microbiome of healthy primiparous cows. Primiparous Holstein cows (n = 20) with no history of clinical mastitis or monthly somatic cell counts >150,000 cells/mL were selected for this study. From each enrolled cow, a composite milk sample was aseptically collected from all 4 mammary quarters (individual quarter somatic cell counts
ISSN:	0022-0302 1525-3198
DOI:	10.3168/jds.2017-14212