Arabidopsis transcription factor WRKY27 influences wilt disease symptom development caused by Ralstonia solanacearum
WRKY transcription factors play a key role in modulating the plant defense transcriptome. Here we show that the Arabidopsis mutant wrky27-1, which lacks a functional WRKY27 transcription factor, showed delayed symptom development in response to the bacterial wilt pathogen Ralstonia solanacearum. Add...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2008-12, Vol.56 (6), p.935-947 |
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| creator | Mukhtar, M. Shahid Deslandes, Laurent Auriac, Marie-Christine Marco, Yves Somssich, Imre E |
| description | WRKY transcription factors play a key role in modulating the plant defense transcriptome. Here we show that the Arabidopsis mutant wrky27-1, which lacks a functional WRKY27 transcription factor, showed delayed symptom development in response to the bacterial wilt pathogen Ralstonia solanacearum. Additionally, wrky27-1 plants did not express PR marker genes upon infection, as also observed in resistant Nd-1 plants. Spatial expression of WRKY27 correlated well with the route of bacterial infection and propagation in planta. Complementation experiments restored both the early wilting phenotype of wild-type Col-1 plants and activation of PR genes, not only when the WRKY27 cDNA is expressed under the control of the native promoter, but also when the SUC2 promoter was used, suggesting that WRKY27 exerts its function in phloem companion cells. Expression studies identified genes involved in nitrogen metabolism and nitric oxide (NO) generation as potential targets of negative regulation by WRKY27. Our results show that WRKY27 negatively influences symptom development of a vascular pathogen, possibly by affecting signaling or trafficking between the phloem and the xylem. |
| doi_str_mv | 10.1111/j.1365-313x.2008.03651.x |
| format | Article |
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Shahid ; Deslandes, Laurent ; Auriac, Marie-Christine ; Marco, Yves ; Somssich, Imre E</creator><creatorcontrib>Mukhtar, M. Shahid ; Deslandes, Laurent ; Auriac, Marie-Christine ; Marco, Yves ; Somssich, Imre E</creatorcontrib><description>WRKY transcription factors play a key role in modulating the plant defense transcriptome. Here we show that the Arabidopsis mutant wrky27-1, which lacks a functional WRKY27 transcription factor, showed delayed symptom development in response to the bacterial wilt pathogen Ralstonia solanacearum. Additionally, wrky27-1 plants did not express PR marker genes upon infection, as also observed in resistant Nd-1 plants. Spatial expression of WRKY27 correlated well with the route of bacterial infection and propagation in planta. Complementation experiments restored both the early wilting phenotype of wild-type Col-1 plants and activation of PR genes, not only when the WRKY27 cDNA is expressed under the control of the native promoter, but also when the SUC2 promoter was used, suggesting that WRKY27 exerts its function in phloem companion cells. Expression studies identified genes involved in nitrogen metabolism and nitric oxide (NO) generation as potential targets of negative regulation by WRKY27. Our results show that WRKY27 negatively influences symptom development of a vascular pathogen, possibly by affecting signaling or trafficking between the phloem and the xylem.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/j.1365-313x.2008.03651.x</identifier><identifier>PMID: 18702671</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Agronomy. Soil science and plant productions ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis - microbiology ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Bacteria ; bacterial wilt ; bacterial wilt disease ; Biological and medical sciences ; Botany ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation, Plant ; Genes, Plant ; Genetic Complementation Test ; Genetics and breeding of economic plants ; Mutagenesis, Insertional ; Mutation ; negative regulator ; Nitric Oxide - biosynthesis ; Nitrogen - metabolism ; Pest resistance ; Phloem - metabolism ; phloem companion cells ; Plant Diseases - microbiology ; Plant pathogens ; Plant pathology ; plant tolerance ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Plants, Genetically Modified - microbiology ; Ralstonia solanacearum ; Ralstonia solanacearum - pathogenicity ; RNA, Plant - metabolism ; SUC2 promoter ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Varietal selection. Specialized plant breeding, plant breeding aims ; xylem</subject><ispartof>The Plant journal : for cell and molecular biology, 2008-12, Vol.56 (6), p.935-947</ispartof><rights>2008 The Authors. 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Shahid</creatorcontrib><creatorcontrib>Deslandes, Laurent</creatorcontrib><creatorcontrib>Auriac, Marie-Christine</creatorcontrib><creatorcontrib>Marco, Yves</creatorcontrib><creatorcontrib>Somssich, Imre E</creatorcontrib><title>Arabidopsis transcription factor WRKY27 influences wilt disease symptom development caused by Ralstonia solanacearum</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>WRKY transcription factors play a key role in modulating the plant defense transcriptome. Here we show that the Arabidopsis mutant wrky27-1, which lacks a functional WRKY27 transcription factor, showed delayed symptom development in response to the bacterial wilt pathogen Ralstonia solanacearum. Additionally, wrky27-1 plants did not express PR marker genes upon infection, as also observed in resistant Nd-1 plants. Spatial expression of WRKY27 correlated well with the route of bacterial infection and propagation in planta. Complementation experiments restored both the early wilting phenotype of wild-type Col-1 plants and activation of PR genes, not only when the WRKY27 cDNA is expressed under the control of the native promoter, but also when the SUC2 promoter was used, suggesting that WRKY27 exerts its function in phloem companion cells. Expression studies identified genes involved in nitrogen metabolism and nitric oxide (NO) generation as potential targets of negative regulation by WRKY27. Our results show that WRKY27 negatively influences symptom development of a vascular pathogen, possibly by affecting signaling or trafficking between the phloem and the xylem.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis - microbiology</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Bacteria</subject><subject>bacterial wilt</subject><subject>bacterial wilt disease</subject><subject>Biological and medical sciences</subject><subject>Botany</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes, Plant</subject><subject>Genetic Complementation Test</subject><subject>Genetics and breeding of economic plants</subject><subject>Mutagenesis, Insertional</subject><subject>Mutation</subject><subject>negative regulator</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitrogen - metabolism</subject><subject>Pest resistance</subject><subject>Phloem - metabolism</subject><subject>phloem companion cells</subject><subject>Plant Diseases - microbiology</subject><subject>Plant pathogens</subject><subject>Plant pathology</subject><subject>plant tolerance</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Plants, Genetically Modified - microbiology</subject><subject>Ralstonia solanacearum</subject><subject>Ralstonia solanacearum - pathogenicity</subject><subject>RNA, Plant - metabolism</subject><subject>SUC2 promoter</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Varietal selection. Specialized plant breeding, plant breeding aims</subject><subject>xylem</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU-P1SAUxYnROG9Gv4ISE2fXClwoZeFiMvH_JJpxJuqKUEoNL22p0Op7317qe2riSjZwc3_ncnIPQpiSkubzbFtSqEQBFHYlI6QuSS5pubuDNr8bn--iDVEVKSSn7ASdprQlhEqo-H10QmtJWCXpBs0X0TS-DVPyCc_RjMlGP80-jLgzdg4Rf7p-94VJ7MeuX9xoXcI_fD_j1idnksNpP0xzGHDrvrs-TIMbZ2zNklyLmz2-Nn2aw-gNTqE3o7HOxGV4gO51ueEeHu8zdPvyxc3l6-Lq_as3lxdXhRWc0AKMMJ1qOUDVdbQGJpUTrWwaw4Hnytq6sdxKgAYAlCCsqVRNCSjaciYEnKHzw9wphm-LS7MefLKuz05cWJJmhCleUZrBJ_-A27DEMXvTjIKgIAXJUH2AbAwpRdfpKfrBxL2mRK-x6K1et6_X7es1Fv0rFr3L0kfH-UszuPav8JhDBp4eAZOs6bschPXpD8eIEoowyNzzA5czcPv_NqBvPrxdX1n_-KDvTNDma8x_3H5khAKhoqqV5PAT8pGycQ</recordid><startdate>200812</startdate><enddate>200812</enddate><creator>Mukhtar, M. Shahid</creator><creator>Deslandes, Laurent</creator><creator>Auriac, Marie-Christine</creator><creator>Marco, Yves</creator><creator>Somssich, Imre E</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>200812</creationdate><title>Arabidopsis transcription factor WRKY27 influences wilt disease symptom development caused by Ralstonia solanacearum</title><author>Mukhtar, M. Shahid ; Deslandes, Laurent ; Auriac, Marie-Christine ; Marco, Yves ; Somssich, Imre E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5401-3a5af9d4336ff183279e5d7bba434327cc8bc4c733b3339502b69810391d42553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis - microbiology</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Bacteria</topic><topic>bacterial wilt</topic><topic>bacterial wilt disease</topic><topic>Biological and medical sciences</topic><topic>Botany</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes, Plant</topic><topic>Genetic Complementation Test</topic><topic>Genetics and breeding of economic plants</topic><topic>Mutagenesis, Insertional</topic><topic>Mutation</topic><topic>negative regulator</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitrogen - metabolism</topic><topic>Pest resistance</topic><topic>Phloem - metabolism</topic><topic>phloem companion cells</topic><topic>Plant Diseases - microbiology</topic><topic>Plant pathogens</topic><topic>Plant pathology</topic><topic>plant tolerance</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Plants, Genetically Modified - microbiology</topic><topic>Ralstonia solanacearum</topic><topic>Ralstonia solanacearum - pathogenicity</topic><topic>RNA, Plant - metabolism</topic><topic>SUC2 promoter</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Varietal selection. 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Shahid</au><au>Deslandes, Laurent</au><au>Auriac, Marie-Christine</au><au>Marco, Yves</au><au>Somssich, Imre E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Arabidopsis transcription factor WRKY27 influences wilt disease symptom development caused by Ralstonia solanacearum</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2008-12</date><risdate>2008</risdate><volume>56</volume><issue>6</issue><spage>935</spage><epage>947</epage><pages>935-947</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>WRKY transcription factors play a key role in modulating the plant defense transcriptome. Here we show that the Arabidopsis mutant wrky27-1, which lacks a functional WRKY27 transcription factor, showed delayed symptom development in response to the bacterial wilt pathogen Ralstonia solanacearum. Additionally, wrky27-1 plants did not express PR marker genes upon infection, as also observed in resistant Nd-1 plants. Spatial expression of WRKY27 correlated well with the route of bacterial infection and propagation in planta. Complementation experiments restored both the early wilting phenotype of wild-type Col-1 plants and activation of PR genes, not only when the WRKY27 cDNA is expressed under the control of the native promoter, but also when the SUC2 promoter was used, suggesting that WRKY27 exerts its function in phloem companion cells. Expression studies identified genes involved in nitrogen metabolism and nitric oxide (NO) generation as potential targets of negative regulation by WRKY27. Our results show that WRKY27 negatively influences symptom development of a vascular pathogen, possibly by affecting signaling or trafficking between the phloem and the xylem.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>18702671</pmid><doi>10.1111/j.1365-313x.2008.03651.x</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Agronomy. Soil science and plant productions Arabidopsis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis - microbiology Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Bacteria bacterial wilt bacterial wilt disease Biological and medical sciences Botany Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Plant Genes, Plant Genetic Complementation Test Genetics and breeding of economic plants Mutagenesis, Insertional Mutation negative regulator Nitric Oxide - biosynthesis Nitrogen - metabolism Pest resistance Phloem - metabolism phloem companion cells Plant Diseases - microbiology Plant pathogens Plant pathology plant tolerance Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Plants, Genetically Modified - microbiology Ralstonia solanacearum Ralstonia solanacearum - pathogenicity RNA, Plant - metabolism SUC2 promoter Transcription Factors - genetics Transcription Factors - metabolism Varietal selection. Specialized plant breeding, plant breeding aims xylem |
| title | Arabidopsis transcription factor WRKY27 influences wilt disease symptom development caused by Ralstonia solanacearum |
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