Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia
Ticks transmit important pathogens affecting cattle such as intracellular bacteria of the genus Anaplasma or protozoa of the order Piroplasmida . This study aimed at assessing tick species present on pastures and cattle and determining occurrence of the tick-borne pathogens Anaplasma phagocytophilum...
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creator | Vasić, Ana Nieder, Marion Zdravković, Nemanja Bojkovski, Jovan Bugarski, Dejan Pavlović, Ivan Silaghi, Cornelia |
description | Ticks transmit important pathogens affecting cattle such as intracellular bacteria of the genus
Anaplasma
or protozoa of the order
Piroplasmida
. This study aimed at assessing tick species present on pastures and cattle and determining occurrence of the tick-borne pathogens
Anaplasma phagocytophilum
and
Babesia
spp. in cattle. During May and June 2013, ticks and EDTA blood were collected from 185 dairy cows at six locations in Serbia. Ticks were also collected directly from the pastures at four of these locations. The occurrence of
A
.
phagocytophilum
was investigated by serological (immunofluorescence antibody test (IFAT)) and molecular methods (real-time PCR) and of piroplasms by traditional PCR followed by sequencing. The most prevalent tick species on animals was
Ixodes ricinus
, (
n
= 167), followed by
Haemaphysalis punctata
(
n
= 146) and
Dermacentor marginatus
(
n
= 122). On the pasture, however, the most common species was
H
.
punctata
(
n
= 41),
I
.
ricinus
(
n
= 37), and
D
.
marginatus
(
n
= 2). Altogether, 4 out of 163 (2.45%) serum samples of cows were positive for
A
.
phagocytophilum
-specific antibodies by IFAT. However, the 135 blood samples tested for
A
.
phagocytophilum
DNA were all negative. Altogether, 5 out of these 135 samples were positive for piroplasm DNA. PCR products were sequenced and identified as a benign
Theileria
spp. with 100% identity with GenBank entries from Italy (
Theileria sergenti
), China (
Theileria
spp.), and Korea (
Theileria buffeli
isolate HS252). The results provide evidence for the presence of several hard tick species infesting cattle in Serbia which can carry pathogens potentially influencing animal health, as well as evidence of contact with tick-borne pathogens
Theileria
spp. and
A
.
phagocytophilum
. |
doi_str_mv | 10.1007/s00436-018-5867-x |
format | Article |
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Anaplasma
or protozoa of the order
Piroplasmida
. This study aimed at assessing tick species present on pastures and cattle and determining occurrence of the tick-borne pathogens
Anaplasma phagocytophilum
and
Babesia
spp. in cattle. During May and June 2013, ticks and EDTA blood were collected from 185 dairy cows at six locations in Serbia. Ticks were also collected directly from the pastures at four of these locations. The occurrence of
A
.
phagocytophilum
was investigated by serological (immunofluorescence antibody test (IFAT)) and molecular methods (real-time PCR) and of piroplasms by traditional PCR followed by sequencing. The most prevalent tick species on animals was
Ixodes ricinus
, (
n
= 167), followed by
Haemaphysalis punctata
(
n
= 146) and
Dermacentor marginatus
(
n
= 122). On the pasture, however, the most common species was
H
.
punctata
(
n
= 41),
I
.
ricinus
(
n
= 37), and
D
.
marginatus
(
n
= 2). Altogether, 4 out of 163 (2.45%) serum samples of cows were positive for
A
.
phagocytophilum
-specific antibodies by IFAT. However, the 135 blood samples tested for
A
.
phagocytophilum
DNA were all negative. Altogether, 5 out of these 135 samples were positive for piroplasm DNA. PCR products were sequenced and identified as a benign
Theileria
spp. with 100% identity with GenBank entries from Italy (
Theileria sergenti
), China (
Theileria
spp.), and Korea (
Theileria buffeli
isolate HS252). The results provide evidence for the presence of several hard tick species infesting cattle in Serbia which can carry pathogens potentially influencing animal health, as well as evidence of contact with tick-borne pathogens
Theileria
spp. and
A
.
phagocytophilum
.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-018-5867-x</identifier><identifier>PMID: 29679202</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Anaplasma phagocytophilum ; Arachnids ; Biomedical and Life Sciences ; Biomedicine ; Bovidae ; Cattle ; Dermacentor marginatus ; Gram-negative bacteria ; Haemaphysalis punctata ; Health aspects ; Host-parasite relationships ; Immunofluorescence ; Immunology ; Medical Microbiology ; Microbiology ; Nucleotide sequence ; Observations ; Original Paper ; Pasture ; Pathogens ; Polymerase chain reaction ; Protozoa ; Species ; Theileria ; Ticks</subject><ispartof>Parasitology research (1987), 2018-06, Vol.117 (6), p.1813-1818</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Copyright Springer Science & Business Media 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-bca80c7e9dcc2b8fb30e672da943b5ab17371926f51b2f8443f2f5a24b8c7b773</citedby><cites>FETCH-LOGICAL-c439t-bca80c7e9dcc2b8fb30e672da943b5ab17371926f51b2f8443f2f5a24b8c7b773</cites><orcidid>0000-0002-0653-2935</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-018-5867-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-018-5867-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29679202$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vasić, Ana</creatorcontrib><creatorcontrib>Nieder, Marion</creatorcontrib><creatorcontrib>Zdravković, Nemanja</creatorcontrib><creatorcontrib>Bojkovski, Jovan</creatorcontrib><creatorcontrib>Bugarski, Dejan</creatorcontrib><creatorcontrib>Pavlović, Ivan</creatorcontrib><creatorcontrib>Silaghi, Cornelia</creatorcontrib><title>Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>Ticks transmit important pathogens affecting cattle such as intracellular bacteria of the genus
Anaplasma
or protozoa of the order
Piroplasmida
. This study aimed at assessing tick species present on pastures and cattle and determining occurrence of the tick-borne pathogens
Anaplasma phagocytophilum
and
Babesia
spp. in cattle. During May and June 2013, ticks and EDTA blood were collected from 185 dairy cows at six locations in Serbia. Ticks were also collected directly from the pastures at four of these locations. The occurrence of
A
.
phagocytophilum
was investigated by serological (immunofluorescence antibody test (IFAT)) and molecular methods (real-time PCR) and of piroplasms by traditional PCR followed by sequencing. The most prevalent tick species on animals was
Ixodes ricinus
, (
n
= 167), followed by
Haemaphysalis punctata
(
n
= 146) and
Dermacentor marginatus
(
n
= 122). On the pasture, however, the most common species was
H
.
punctata
(
n
= 41),
I
.
ricinus
(
n
= 37), and
D
.
marginatus
(
n
= 2). Altogether, 4 out of 163 (2.45%) serum samples of cows were positive for
A
.
phagocytophilum
-specific antibodies by IFAT. However, the 135 blood samples tested for
A
.
phagocytophilum
DNA were all negative. Altogether, 5 out of these 135 samples were positive for piroplasm DNA. PCR products were sequenced and identified as a benign
Theileria
spp. with 100% identity with GenBank entries from Italy (
Theileria sergenti
), China (
Theileria
spp.), and Korea (
Theileria buffeli
isolate HS252). The results provide evidence for the presence of several hard tick species infesting cattle in Serbia which can carry pathogens potentially influencing animal health, as well as evidence of contact with tick-borne pathogens
Theileria
spp. and
A
.
phagocytophilum
.</description><subject>Anaplasma phagocytophilum</subject><subject>Arachnids</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bovidae</subject><subject>Cattle</subject><subject>Dermacentor marginatus</subject><subject>Gram-negative bacteria</subject><subject>Haemaphysalis punctata</subject><subject>Health aspects</subject><subject>Host-parasite relationships</subject><subject>Immunofluorescence</subject><subject>Immunology</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Nucleotide sequence</subject><subject>Observations</subject><subject>Original Paper</subject><subject>Pasture</subject><subject>Pathogens</subject><subject>Polymerase chain reaction</subject><subject>Protozoa</subject><subject>Species</subject><subject>Theileria</subject><subject>Ticks</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kU9v1DAQxS1ERZfCB-CCInHpJa3_JY6Pq4q2SJWQoJwt2xnvuiR2sBOp_fY43UIFAvngkef3nmb8EHpH8BnBWJxnjDlra0y6uulaUd-_QBvCGa2JbJqXaINlqTEh7Bi9zvkOYyJazl-hYypbISmmGxRvvf1e-eAgz3r2MVQ69FW0dkkJgoUqumob9DToPOpq2utdtA9znPZ-WMZHdvIpPrZzsamsnucB1mreQ_UFpsUM3q4uXyEZr9-gI6eHDG-f7hP07fLj7cV1ffP56tPF9qa2nMm5NlZ32AqQvbXUdM4wDK2gvZacmUYbIpggkrauIYa6jnPmqGs05aazwgjBTtDpwXdK8cdSllOjzxaGQQeIS1Zl-U42jHJZ0A9_oXdxSaFMVyjWYYYFEc_UTg-gyofFOWm7mqptw8oEuKFtoc7-QZXTw-htDOB8ef9DQA4Cm2LOCZyakh91elAEqzVkdQhZlZDVGrK6L5r3TwMvZoT-t-JXqgWgByCXVthBet7o_64_AcO_sZ0</recordid><startdate>20180601</startdate><enddate>20180601</enddate><creator>Vasić, Ana</creator><creator>Nieder, Marion</creator><creator>Zdravković, Nemanja</creator><creator>Bojkovski, Jovan</creator><creator>Bugarski, Dejan</creator><creator>Pavlović, Ivan</creator><creator>Silaghi, Cornelia</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0653-2935</orcidid></search><sort><creationdate>20180601</creationdate><title>Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia</title><author>Vasić, Ana ; Nieder, Marion ; Zdravković, Nemanja ; Bojkovski, Jovan ; Bugarski, Dejan ; Pavlović, Ivan ; Silaghi, Cornelia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-bca80c7e9dcc2b8fb30e672da943b5ab17371926f51b2f8443f2f5a24b8c7b773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Anaplasma phagocytophilum</topic><topic>Arachnids</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bovidae</topic><topic>Cattle</topic><topic>Dermacentor marginatus</topic><topic>Gram-negative bacteria</topic><topic>Haemaphysalis punctata</topic><topic>Health aspects</topic><topic>Host-parasite relationships</topic><topic>Immunofluorescence</topic><topic>Immunology</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Nucleotide sequence</topic><topic>Observations</topic><topic>Original Paper</topic><topic>Pasture</topic><topic>Pathogens</topic><topic>Polymerase chain reaction</topic><topic>Protozoa</topic><topic>Species</topic><topic>Theileria</topic><topic>Ticks</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vasić, Ana</creatorcontrib><creatorcontrib>Nieder, Marion</creatorcontrib><creatorcontrib>Zdravković, Nemanja</creatorcontrib><creatorcontrib>Bojkovski, Jovan</creatorcontrib><creatorcontrib>Bugarski, Dejan</creatorcontrib><creatorcontrib>Pavlović, Ivan</creatorcontrib><creatorcontrib>Silaghi, Cornelia</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vasić, Ana</au><au>Nieder, Marion</au><au>Zdravković, Nemanja</au><au>Bojkovski, Jovan</au><au>Bugarski, Dejan</au><au>Pavlović, Ivan</au><au>Silaghi, Cornelia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2018-06-01</date><risdate>2018</risdate><volume>117</volume><issue>6</issue><spage>1813</spage><epage>1818</epage><pages>1813-1818</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>Ticks transmit important pathogens affecting cattle such as intracellular bacteria of the genus
Anaplasma
or protozoa of the order
Piroplasmida
. This study aimed at assessing tick species present on pastures and cattle and determining occurrence of the tick-borne pathogens
Anaplasma phagocytophilum
and
Babesia
spp. in cattle. During May and June 2013, ticks and EDTA blood were collected from 185 dairy cows at six locations in Serbia. Ticks were also collected directly from the pastures at four of these locations. The occurrence of
A
.
phagocytophilum
was investigated by serological (immunofluorescence antibody test (IFAT)) and molecular methods (real-time PCR) and of piroplasms by traditional PCR followed by sequencing. The most prevalent tick species on animals was
Ixodes ricinus
, (
n
= 167), followed by
Haemaphysalis punctata
(
n
= 146) and
Dermacentor marginatus
(
n
= 122). On the pasture, however, the most common species was
H
.
punctata
(
n
= 41),
I
.
ricinus
(
n
= 37), and
D
.
marginatus
(
n
= 2). Altogether, 4 out of 163 (2.45%) serum samples of cows were positive for
A
.
phagocytophilum
-specific antibodies by IFAT. However, the 135 blood samples tested for
A
.
phagocytophilum
DNA were all negative. Altogether, 5 out of these 135 samples were positive for piroplasm DNA. PCR products were sequenced and identified as a benign
Theileria
spp. with 100% identity with GenBank entries from Italy (
Theileria sergenti
), China (
Theileria
spp.), and Korea (
Theileria buffeli
isolate HS252). The results provide evidence for the presence of several hard tick species infesting cattle in Serbia which can carry pathogens potentially influencing animal health, as well as evidence of contact with tick-borne pathogens
Theileria
spp. and
A
.
phagocytophilum
.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29679202</pmid><doi>10.1007/s00436-018-5867-x</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-0653-2935</orcidid></addata></record> |
fulltext | fulltext |
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ispartof | Parasitology research (1987), 2018-06, Vol.117 (6), p.1813-1818 |
issn | 0932-0113 1432-1955 |
language | eng |
recordid | cdi_proquest_miscellaneous_2028953249 |
source | SpringerNature Journals |
subjects | Anaplasma phagocytophilum Arachnids Biomedical and Life Sciences Biomedicine Bovidae Cattle Dermacentor marginatus Gram-negative bacteria Haemaphysalis punctata Health aspects Host-parasite relationships Immunofluorescence Immunology Medical Microbiology Microbiology Nucleotide sequence Observations Original Paper Pasture Pathogens Polymerase chain reaction Protozoa Species Theileria Ticks |
title | Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia |
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