Cytotoxicity of novel fluoride solutions and their influence on mineral loss from enamel exposed to a Streptococcus mutans biofilm
•The new fluoridated nanocomplexes presented a non-cytotoxic behaviour.•Hydroxypropyl-β-cyclodextrin/γ-cyclodextrin with TiF4 decreased enamel loss.•All solutions showed inhibitory properties against S. mutans. This study evaluated the cytotoxicity, antimicrobial activity and in vitro influence of n...
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| Veröffentlicht in: | Archives of oral biology 2018-07, Vol.91, p.57-62 |
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| creator | Vieira, Thiago Isidro Câmara, João Victor Frazão Cardoso, Júlia Gabiroboertz Alexandria, Adílis Kalina Pintor, Andréa Vaz Braga Villaça, Jaqueline Correia Cabral, Lúcio Mendes Romanos, Maria Teresa Villela Fonseca-Gonçalves, Andrea Valença, Ana Maria Gondim Maia, Lucianne Cople |
| description | •The new fluoridated nanocomplexes presented a non-cytotoxic behaviour.•Hydroxypropyl-β-cyclodextrin/γ-cyclodextrin with TiF4 decreased enamel loss.•All solutions showed inhibitory properties against S. mutans.
This study evaluated the cytotoxicity, antimicrobial activity and in vitro influence of new fluoridated nanocomplexes on dental demineralization.
The nanocomplexes hydroxypropyl-β-cyclodextrin with 1% titanium tetrafluoride (TiF4) and γ-cyclodextrin with TiF4 were compared to a positive control (TiF4), a blank control (without treatment) and negative controls (hydroxypropyl-β-cyclodextrin, γ-cyclodextrin, deionized water), following 12- and 72-hour complexation periods. The cytotoxicity was assessed using the neutral red dye uptake assay at T1–15 min, T2–30 min and T3–24 h. A minimum bactericidal concentration (MBC) against Streptococcus mutans (ATCC 25175) was performed. Enamel blocks were exposed to an S. mutans biofilm, and the percentage of surface microhardness loss was obtained. Biocompatibility and microhardness data were analysed using ANOVA/Tukey tests (p |
| doi_str_mv | 10.1016/j.archoralbio.2018.04.008 |
| format | Article |
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This study evaluated the cytotoxicity, antimicrobial activity and in vitro influence of new fluoridated nanocomplexes on dental demineralization.
The nanocomplexes hydroxypropyl-β-cyclodextrin with 1% titanium tetrafluoride (TiF4) and γ-cyclodextrin with TiF4 were compared to a positive control (TiF4), a blank control (without treatment) and negative controls (hydroxypropyl-β-cyclodextrin, γ-cyclodextrin, deionized water), following 12- and 72-hour complexation periods. The cytotoxicity was assessed using the neutral red dye uptake assay at T1–15 min, T2–30 min and T3–24 h. A minimum bactericidal concentration (MBC) against Streptococcus mutans (ATCC 25175) was performed. Enamel blocks were exposed to an S. mutans biofilm, and the percentage of surface microhardness loss was obtained. Biocompatibility and microhardness data were analysed using ANOVA/Tukey tests (p < 0.05).
At T1, the cell viability results of the nanocomplexes were similar to that of the blank control. At T2 and T3, the 72 h nanocomplexes demonstrated cell viability results similar to that of the blank, while the 12 h solutions showed results different from that of the blank (p < 0.05). All fluoridated nanocompounds inhibited S. mutans (MBC = 0.25%), while the MBC of TiF4 alone was 0.13%. All fluoridated compounds presented a percentage of surface microhardness loss lower than that of deionized water (p < 0.05).
The new fluoridated nanocomplexes did not induce critical cytotoxic effects during the experimental periods, whilst they did show bactericidal potential against S. mutans and inhibited enamel mineral loss.</description><identifier>ISSN: 0003-9969</identifier><identifier>EISSN: 1879-1506</identifier><identifier>DOI: 10.1016/j.archoralbio.2018.04.008</identifier><identifier>PMID: 29679886</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Dental caries ; Dentistry ; Fluoride ; Nanotechnology ; S. mutans biofilm</subject><ispartof>Archives of oral biology, 2018-07, Vol.91, p.57-62</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-31a58f9ed8b273e050a31a2168bdfd49da875f4fc9e72dbba781b12d34e6fa193</citedby><cites>FETCH-LOGICAL-c428t-31a58f9ed8b273e050a31a2168bdfd49da875f4fc9e72dbba781b12d34e6fa193</cites><orcidid>0000-0003-1026-9401 ; 0000-0002-9687-4401 ; 0000-0001-6467-7078</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.archoralbio.2018.04.008$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29679886$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vieira, Thiago Isidro</creatorcontrib><creatorcontrib>Câmara, João Victor Frazão</creatorcontrib><creatorcontrib>Cardoso, Júlia Gabiroboertz</creatorcontrib><creatorcontrib>Alexandria, Adílis Kalina</creatorcontrib><creatorcontrib>Pintor, Andréa Vaz Braga</creatorcontrib><creatorcontrib>Villaça, Jaqueline Correia</creatorcontrib><creatorcontrib>Cabral, Lúcio Mendes</creatorcontrib><creatorcontrib>Romanos, Maria Teresa Villela</creatorcontrib><creatorcontrib>Fonseca-Gonçalves, Andrea</creatorcontrib><creatorcontrib>Valença, Ana Maria Gondim</creatorcontrib><creatorcontrib>Maia, Lucianne Cople</creatorcontrib><title>Cytotoxicity of novel fluoride solutions and their influence on mineral loss from enamel exposed to a Streptococcus mutans biofilm</title><title>Archives of oral biology</title><addtitle>Arch Oral Biol</addtitle><description>•The new fluoridated nanocomplexes presented a non-cytotoxic behaviour.•Hydroxypropyl-β-cyclodextrin/γ-cyclodextrin with TiF4 decreased enamel loss.•All solutions showed inhibitory properties against S. mutans.
This study evaluated the cytotoxicity, antimicrobial activity and in vitro influence of new fluoridated nanocomplexes on dental demineralization.
The nanocomplexes hydroxypropyl-β-cyclodextrin with 1% titanium tetrafluoride (TiF4) and γ-cyclodextrin with TiF4 were compared to a positive control (TiF4), a blank control (without treatment) and negative controls (hydroxypropyl-β-cyclodextrin, γ-cyclodextrin, deionized water), following 12- and 72-hour complexation periods. The cytotoxicity was assessed using the neutral red dye uptake assay at T1–15 min, T2–30 min and T3–24 h. A minimum bactericidal concentration (MBC) against Streptococcus mutans (ATCC 25175) was performed. Enamel blocks were exposed to an S. mutans biofilm, and the percentage of surface microhardness loss was obtained. Biocompatibility and microhardness data were analysed using ANOVA/Tukey tests (p < 0.05).
At T1, the cell viability results of the nanocomplexes were similar to that of the blank control. At T2 and T3, the 72 h nanocomplexes demonstrated cell viability results similar to that of the blank, while the 12 h solutions showed results different from that of the blank (p < 0.05). All fluoridated nanocompounds inhibited S. mutans (MBC = 0.25%), while the MBC of TiF4 alone was 0.13%. All fluoridated compounds presented a percentage of surface microhardness loss lower than that of deionized water (p < 0.05).
The new fluoridated nanocomplexes did not induce critical cytotoxic effects during the experimental periods, whilst they did show bactericidal potential against S. mutans and inhibited enamel mineral loss.</description><subject>Dental caries</subject><subject>Dentistry</subject><subject>Fluoride</subject><subject>Nanotechnology</subject><subject>S. mutans biofilm</subject><issn>0003-9969</issn><issn>1879-1506</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNqNUU1v1DAQtRCIbgt_AZkblwTbSRz7iFZ8VKrEAThbjj1WvUoyi-1U3Su_HK-2II6cRjPz3puPR8hbzlrOuHx_aG1y95jsPEVsBeOqZX3LmHpGdlyNuuEDk8_JjjHWNVpLfUWucz7UdJCSvyRXQstRKyV35Nf-VLDgY3SxnCgGuuIDzDTMG6bogWactxJxzdSunpZ7iInGtbZhdUBxpUtcoS5CZ8yZhoQLhdUuVQIej5ihcpBa-q0kOBZ06NyW6bIVWxXr8iHOyyvyItg5w-uneEN-fPr4ff-lufv6-Xb_4a5xvVCl6bgdVNDg1STGDtjAbC0JLtXkg--1t2ocQh-chlH4abKj4hMXvutBBst1d0PeXXSPCX9ukItZYnYwz3YF3LIRTCjdj8MoKlRfoC7VsxIEc0xxselkODNnC8zB_GOBOVtgWG-qBZX75mnMNi3g_zL__LwC9hcA1GMfIiSTXTy_08cErhiP8T_G_AbCVaHd</recordid><startdate>201807</startdate><enddate>201807</enddate><creator>Vieira, Thiago Isidro</creator><creator>Câmara, João Victor Frazão</creator><creator>Cardoso, Júlia Gabiroboertz</creator><creator>Alexandria, Adílis Kalina</creator><creator>Pintor, Andréa Vaz Braga</creator><creator>Villaça, Jaqueline Correia</creator><creator>Cabral, Lúcio Mendes</creator><creator>Romanos, Maria Teresa Villela</creator><creator>Fonseca-Gonçalves, Andrea</creator><creator>Valença, Ana Maria Gondim</creator><creator>Maia, Lucianne Cople</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1026-9401</orcidid><orcidid>https://orcid.org/0000-0002-9687-4401</orcidid><orcidid>https://orcid.org/0000-0001-6467-7078</orcidid></search><sort><creationdate>201807</creationdate><title>Cytotoxicity of novel fluoride solutions and their influence on mineral loss from enamel exposed to a Streptococcus mutans biofilm</title><author>Vieira, Thiago Isidro ; Câmara, João Victor Frazão ; Cardoso, Júlia Gabiroboertz ; Alexandria, Adílis Kalina ; Pintor, Andréa Vaz Braga ; Villaça, Jaqueline Correia ; Cabral, Lúcio Mendes ; Romanos, Maria Teresa Villela ; Fonseca-Gonçalves, Andrea ; Valença, Ana Maria Gondim ; Maia, Lucianne Cople</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-31a58f9ed8b273e050a31a2168bdfd49da875f4fc9e72dbba781b12d34e6fa193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Dental caries</topic><topic>Dentistry</topic><topic>Fluoride</topic><topic>Nanotechnology</topic><topic>S. mutans biofilm</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vieira, Thiago Isidro</creatorcontrib><creatorcontrib>Câmara, João Victor Frazão</creatorcontrib><creatorcontrib>Cardoso, Júlia Gabiroboertz</creatorcontrib><creatorcontrib>Alexandria, Adílis Kalina</creatorcontrib><creatorcontrib>Pintor, Andréa Vaz Braga</creatorcontrib><creatorcontrib>Villaça, Jaqueline Correia</creatorcontrib><creatorcontrib>Cabral, Lúcio Mendes</creatorcontrib><creatorcontrib>Romanos, Maria Teresa Villela</creatorcontrib><creatorcontrib>Fonseca-Gonçalves, Andrea</creatorcontrib><creatorcontrib>Valença, Ana Maria Gondim</creatorcontrib><creatorcontrib>Maia, Lucianne Cople</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of oral biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vieira, Thiago Isidro</au><au>Câmara, João Victor Frazão</au><au>Cardoso, Júlia Gabiroboertz</au><au>Alexandria, Adílis Kalina</au><au>Pintor, Andréa Vaz Braga</au><au>Villaça, Jaqueline Correia</au><au>Cabral, Lúcio Mendes</au><au>Romanos, Maria Teresa Villela</au><au>Fonseca-Gonçalves, Andrea</au><au>Valença, Ana Maria Gondim</au><au>Maia, Lucianne Cople</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytotoxicity of novel fluoride solutions and their influence on mineral loss from enamel exposed to a Streptococcus mutans biofilm</atitle><jtitle>Archives of oral biology</jtitle><addtitle>Arch Oral Biol</addtitle><date>2018-07</date><risdate>2018</risdate><volume>91</volume><spage>57</spage><epage>62</epage><pages>57-62</pages><issn>0003-9969</issn><eissn>1879-1506</eissn><abstract>•The new fluoridated nanocomplexes presented a non-cytotoxic behaviour.•Hydroxypropyl-β-cyclodextrin/γ-cyclodextrin with TiF4 decreased enamel loss.•All solutions showed inhibitory properties against S. mutans.
This study evaluated the cytotoxicity, antimicrobial activity and in vitro influence of new fluoridated nanocomplexes on dental demineralization.
The nanocomplexes hydroxypropyl-β-cyclodextrin with 1% titanium tetrafluoride (TiF4) and γ-cyclodextrin with TiF4 were compared to a positive control (TiF4), a blank control (without treatment) and negative controls (hydroxypropyl-β-cyclodextrin, γ-cyclodextrin, deionized water), following 12- and 72-hour complexation periods. The cytotoxicity was assessed using the neutral red dye uptake assay at T1–15 min, T2–30 min and T3–24 h. A minimum bactericidal concentration (MBC) against Streptococcus mutans (ATCC 25175) was performed. Enamel blocks were exposed to an S. mutans biofilm, and the percentage of surface microhardness loss was obtained. Biocompatibility and microhardness data were analysed using ANOVA/Tukey tests (p < 0.05).
At T1, the cell viability results of the nanocomplexes were similar to that of the blank control. At T2 and T3, the 72 h nanocomplexes demonstrated cell viability results similar to that of the blank, while the 12 h solutions showed results different from that of the blank (p < 0.05). All fluoridated nanocompounds inhibited S. mutans (MBC = 0.25%), while the MBC of TiF4 alone was 0.13%. All fluoridated compounds presented a percentage of surface microhardness loss lower than that of deionized water (p < 0.05).
The new fluoridated nanocomplexes did not induce critical cytotoxic effects during the experimental periods, whilst they did show bactericidal potential against S. mutans and inhibited enamel mineral loss.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>29679886</pmid><doi>10.1016/j.archoralbio.2018.04.008</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-1026-9401</orcidid><orcidid>https://orcid.org/0000-0002-9687-4401</orcidid><orcidid>https://orcid.org/0000-0001-6467-7078</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Archives of oral biology, 2018-07, Vol.91, p.57-62 |
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| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Dental caries Dentistry Fluoride Nanotechnology S. mutans biofilm |
| title | Cytotoxicity of novel fluoride solutions and their influence on mineral loss from enamel exposed to a Streptococcus mutans biofilm |
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