Molecular identification of mesophilic and psychrotrophic bacteria from raw cow's milk

The aim of this study was to use molecular techniques to assess the microbiota of eight raw cow's milk samples at biotype and species level. Sixty-six isolates from raw milk samples were screened by Randomly amplified polymorphic DNA–PCR (RAPD–PCR) biotyping and representative strains of RAPD–P...

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Veröffentlicht in:	Food microbiology 2009-04, Vol.26 (2), p.228-231
Hauptverfasser:	Ercolini, Danilo, Russo, Federica, Ferrocino, Ilario, Villani, Francesco
Format:	Artikel
Sprache:	eng
Schlagworte:	16S rRNA gene sequencing Animals bacterial contamination Biological and medical sciences biotypes Cattle Citrobacter freundii Colony Count, Microbial Enterobacteriaceae Enterobacteriaceae - genetics Enterobacteriaceae - growth & development Enterobacteriaceae - isolation & purification Food Contamination - analysis Food Contamination - prevention & control Food industries Food Microbiology Food Preservation - methods Food Preservation - standards food spoilage Fundamental and applied biological sciences. Psychology Gram-negative bacteria Gram-positive bacteria Hafnia alvei Humans isolation Lactococcus Lactococcus - genetics Lactococcus - growth & development Lactococcus - isolation & purification microbial detection Milk - microbiology Milk and cheese industries. Ice creams Molecular methods molecular sequence data molecular systematics nucleotide sequences proteolysis Pseudomonas Pseudomonas - genetics Pseudomonas - growth & development Pseudomonas - isolation & purification Psychrotrophic bacteria Random Amplified Polymorphic DNA Technique Raw milk RNA, Ribosomal, 16S - chemistry RNA, Ribosomal, 16S - genetics Serratia marcescens Species Specificity Spoilage bacteria Staphylococcus Staphylococcus - genetics Staphylococcus - growth & development Staphylococcus - isolation & purification Temperature
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