Eriodictyol protects against Staphylococcus aureus-induced lung cell injury by inhibiting alpha-hemolysin expression
Staphylococcus aureus ( S. aureus ) is a common pathogenic bacterium that causes various diseases in both humans and animals. With the increased prevalence of methicillin-resistant S. aureus , the therapeutic effects of commonly used antibiotics are limited against S. aureus infection. Novel treatme...
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| Veröffentlicht in: | World journal of microbiology & biotechnology 2018-05, Vol.34 (5), p.64-7, Article 64 |
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| creator | Xuewen, He Ping, Ouyang Zhongwei, Yuan Zhongqiong, Yin Hualin, Fu Juchun, Lin Changliang, He Gang, Shu Zhixiang, Yuan Xu, Song Yuanfeng, Zou Lixia, Li Lizi, Yin |
| description | Staphylococcus aureus
(
S. aureus
) is a common pathogenic bacterium that causes various diseases in both humans and animals. With the increased prevalence of methicillin-resistant
S. aureus
, the therapeutic effects of commonly used antibiotics are limited against
S. aureus
infection. Novel treatment strategies and new antibiotics are needed urgently to address this concern. Many studies have shown that virulence factors secreted from
S. aureus
play vital roles in their pathogenic processes. Alpha-hemolysin (Hla), an important exotoxin in
S. aureus
, is one such virulence factor that increases sensitivity of multiple host cells to
S. aureus
resulting in various diseases. Eriodictyol is a flavonoid compound that exists in many fruits and vegetables. In this study, eriodictyol was demonstrated to inhibit the expression of Hla by hemolysis assays, western blotting, and RT-qPCR at the sub-minimal inhibitory concentration. In live/dead and cytotoxicity assays, the results showed that eriodictyol protected A549 cells against Hla-induced injury in a dose-dependent manner. The minimal inhibitory concentration of eriodictyol against
S. aureus
was 512 µg/mL. Eriodictyol can downregulate
S. aureus
Hla at both the expressional and transcriptional levels without affecting
S. aureus
growth. In addition, cell assays had proved that eriodictyol could protect A549 cells against Hla damage. Eriodictyol could therefore have the potential to treat
S. aureus
infection targeting Hla. |
| doi_str_mv | 10.1007/s11274-018-2446-3 |
| format | Article |
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(
S. aureus
) is a common pathogenic bacterium that causes various diseases in both humans and animals. With the increased prevalence of methicillin-resistant
S. aureus
, the therapeutic effects of commonly used antibiotics are limited against
S. aureus
infection. Novel treatment strategies and new antibiotics are needed urgently to address this concern. Many studies have shown that virulence factors secreted from
S. aureus
play vital roles in their pathogenic processes. Alpha-hemolysin (Hla), an important exotoxin in
S. aureus
, is one such virulence factor that increases sensitivity of multiple host cells to
S. aureus
resulting in various diseases. Eriodictyol is a flavonoid compound that exists in many fruits and vegetables. In this study, eriodictyol was demonstrated to inhibit the expression of Hla by hemolysis assays, western blotting, and RT-qPCR at the sub-minimal inhibitory concentration. In live/dead and cytotoxicity assays, the results showed that eriodictyol protected A549 cells against Hla-induced injury in a dose-dependent manner. The minimal inhibitory concentration of eriodictyol against
S. aureus
was 512 µg/mL. Eriodictyol can downregulate
S. aureus
Hla at both the expressional and transcriptional levels without affecting
S. aureus
growth. In addition, cell assays had proved that eriodictyol could protect A549 cells against Hla damage. Eriodictyol could therefore have the potential to treat
S. aureus
infection targeting Hla.</description><identifier>ISSN: 0959-3993</identifier><identifier>EISSN: 1573-0972</identifier><identifier>DOI: 10.1007/s11274-018-2446-3</identifier><identifier>PMID: 29671126</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>A549 Cells - drug effects ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Applied Microbiology ; Assaying ; Bacterial Toxins - genetics ; Bacterial Toxins - metabolism ; Bacterial Toxins - toxicity ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cell injury ; Cytotoxicity ; Down-Regulation - drug effects ; Drug resistance ; Environmental Engineering/Biotechnology ; Exotoxins ; Flavanones - pharmacology ; Gene Expression Regulation, Bacterial - drug effects ; Gram-positive bacteria ; Hemolysin Proteins - drug effects ; Hemolysin Proteins - genetics ; Hemolysin Proteins - metabolism ; Hemolysin Proteins - toxicity ; Hemolysis ; Humans ; Life Sciences ; Lung Injury - microbiology ; Lung Injury - prevention & control ; Lungs ; Methicillin ; Methicillin-Resistant Staphylococcus aureus - drug effects ; Methicillin-Resistant Staphylococcus aureus - metabolism ; Microbial Sensitivity Tests ; Microbiology ; Original Paper ; Pathogens ; Penicillin ; Pneumonia, Staphylococcal - microbiology ; Pneumonia, Staphylococcal - pathology ; Pneumonia, Staphylococcal - prevention & control ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - growth & development ; Staphylococcus aureus - metabolism ; Staphylococcus aureus - pathogenicity ; Toxicity ; Transcription ; Vegetables ; Virulence ; Virulence factors ; Virulence Factors - metabolism ; Western blotting</subject><ispartof>World journal of microbiology & biotechnology, 2018-05, Vol.34 (5), p.64-7, Article 64</ispartof><rights>Springer Science+Business Media B.V., part of Springer Nature 2018</rights><rights>World Journal of Microbiology and Biotechnology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-6726775d9f473305042d0cc8588bc0067beaa6020f6a6753afdf7669c59be5be3</citedby><cites>FETCH-LOGICAL-c409t-6726775d9f473305042d0cc8588bc0067beaa6020f6a6753afdf7669c59be5be3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11274-018-2446-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11274-018-2446-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29671126$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xuewen, He</creatorcontrib><creatorcontrib>Ping, Ouyang</creatorcontrib><creatorcontrib>Zhongwei, Yuan</creatorcontrib><creatorcontrib>Zhongqiong, Yin</creatorcontrib><creatorcontrib>Hualin, Fu</creatorcontrib><creatorcontrib>Juchun, Lin</creatorcontrib><creatorcontrib>Changliang, He</creatorcontrib><creatorcontrib>Gang, Shu</creatorcontrib><creatorcontrib>Zhixiang, Yuan</creatorcontrib><creatorcontrib>Xu, Song</creatorcontrib><creatorcontrib>Yuanfeng, Zou</creatorcontrib><creatorcontrib>Lixia, Li</creatorcontrib><creatorcontrib>Lizi, Yin</creatorcontrib><title>Eriodictyol protects against Staphylococcus aureus-induced lung cell injury by inhibiting alpha-hemolysin expression</title><title>World journal of microbiology & biotechnology</title><addtitle>World J Microbiol Biotechnol</addtitle><addtitle>World J Microbiol Biotechnol</addtitle><description>Staphylococcus aureus
(
S. aureus
) is a common pathogenic bacterium that causes various diseases in both humans and animals. With the increased prevalence of methicillin-resistant
S. aureus
, the therapeutic effects of commonly used antibiotics are limited against
S. aureus
infection. Novel treatment strategies and new antibiotics are needed urgently to address this concern. Many studies have shown that virulence factors secreted from
S. aureus
play vital roles in their pathogenic processes. Alpha-hemolysin (Hla), an important exotoxin in
S. aureus
, is one such virulence factor that increases sensitivity of multiple host cells to
S. aureus
resulting in various diseases. Eriodictyol is a flavonoid compound that exists in many fruits and vegetables. In this study, eriodictyol was demonstrated to inhibit the expression of Hla by hemolysis assays, western blotting, and RT-qPCR at the sub-minimal inhibitory concentration. In live/dead and cytotoxicity assays, the results showed that eriodictyol protected A549 cells against Hla-induced injury in a dose-dependent manner. The minimal inhibitory concentration of eriodictyol against
S. aureus
was 512 µg/mL. Eriodictyol can downregulate
S. aureus
Hla at both the expressional and transcriptional levels without affecting
S. aureus
growth. In addition, cell assays had proved that eriodictyol could protect A549 cells against Hla damage. Eriodictyol could therefore have the potential to treat
S. aureus
infection targeting Hla.</description><subject>A549 Cells - drug effects</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Applied Microbiology</subject><subject>Assaying</subject><subject>Bacterial Toxins - genetics</subject><subject>Bacterial Toxins - metabolism</subject><subject>Bacterial Toxins - toxicity</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell injury</subject><subject>Cytotoxicity</subject><subject>Down-Regulation - drug effects</subject><subject>Drug resistance</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Exotoxins</subject><subject>Flavanones - pharmacology</subject><subject>Gene Expression Regulation, Bacterial - drug effects</subject><subject>Gram-positive bacteria</subject><subject>Hemolysin Proteins - drug effects</subject><subject>Hemolysin Proteins - genetics</subject><subject>Hemolysin Proteins - metabolism</subject><subject>Hemolysin Proteins - toxicity</subject><subject>Hemolysis</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Lung Injury - microbiology</subject><subject>Lung Injury - prevention & control</subject><subject>Lungs</subject><subject>Methicillin</subject><subject>Methicillin-Resistant Staphylococcus aureus - drug effects</subject><subject>Methicillin-Resistant Staphylococcus aureus - metabolism</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Original Paper</subject><subject>Pathogens</subject><subject>Penicillin</subject><subject>Pneumonia, Staphylococcal - microbiology</subject><subject>Pneumonia, Staphylococcal - pathology</subject><subject>Pneumonia, Staphylococcal - prevention & control</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - growth & development</subject><subject>Staphylococcus aureus - metabolism</subject><subject>Staphylococcus aureus - pathogenicity</subject><subject>Toxicity</subject><subject>Transcription</subject><subject>Vegetables</subject><subject>Virulence</subject><subject>Virulence factors</subject><subject>Virulence Factors - metabolism</subject><subject>Western 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protects against Staphylococcus aureus-induced lung cell injury by inhibiting alpha-hemolysin expression</title><author>Xuewen, He ; Ping, Ouyang ; Zhongwei, Yuan ; Zhongqiong, Yin ; Hualin, Fu ; Juchun, Lin ; Changliang, He ; Gang, Shu ; Zhixiang, Yuan ; Xu, Song ; Yuanfeng, Zou ; Lixia, Li ; Lizi, Yin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-6726775d9f473305042d0cc8588bc0067beaa6020f6a6753afdf7669c59be5be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>A549 Cells - drug effects</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Applied Microbiology</topic><topic>Assaying</topic><topic>Bacterial Toxins - genetics</topic><topic>Bacterial Toxins - metabolism</topic><topic>Bacterial Toxins - toxicity</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell injury</topic><topic>Cytotoxicity</topic><topic>Down-Regulation - drug effects</topic><topic>Drug resistance</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Exotoxins</topic><topic>Flavanones - pharmacology</topic><topic>Gene Expression Regulation, Bacterial - drug effects</topic><topic>Gram-positive bacteria</topic><topic>Hemolysin Proteins - drug effects</topic><topic>Hemolysin Proteins - genetics</topic><topic>Hemolysin Proteins - metabolism</topic><topic>Hemolysin Proteins - toxicity</topic><topic>Hemolysis</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Lung Injury - microbiology</topic><topic>Lung Injury - prevention & control</topic><topic>Lungs</topic><topic>Methicillin</topic><topic>Methicillin-Resistant Staphylococcus aureus - drug effects</topic><topic>Methicillin-Resistant Staphylococcus aureus - metabolism</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Original 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Microbiol Biotechnol</addtitle><date>2018-05-01</date><risdate>2018</risdate><volume>34</volume><issue>5</issue><spage>64</spage><epage>7</epage><pages>64-7</pages><artnum>64</artnum><issn>0959-3993</issn><eissn>1573-0972</eissn><abstract>Staphylococcus aureus
(
S. aureus
) is a common pathogenic bacterium that causes various diseases in both humans and animals. With the increased prevalence of methicillin-resistant
S. aureus
, the therapeutic effects of commonly used antibiotics are limited against
S. aureus
infection. Novel treatment strategies and new antibiotics are needed urgently to address this concern. Many studies have shown that virulence factors secreted from
S. aureus
play vital roles in their pathogenic processes. Alpha-hemolysin (Hla), an important exotoxin in
S. aureus
, is one such virulence factor that increases sensitivity of multiple host cells to
S. aureus
resulting in various diseases. Eriodictyol is a flavonoid compound that exists in many fruits and vegetables. In this study, eriodictyol was demonstrated to inhibit the expression of Hla by hemolysis assays, western blotting, and RT-qPCR at the sub-minimal inhibitory concentration. In live/dead and cytotoxicity assays, the results showed that eriodictyol protected A549 cells against Hla-induced injury in a dose-dependent manner. The minimal inhibitory concentration of eriodictyol against
S. aureus
was 512 µg/mL. Eriodictyol can downregulate
S. aureus
Hla at both the expressional and transcriptional levels without affecting
S. aureus
growth. In addition, cell assays had proved that eriodictyol could protect A549 cells against Hla damage. Eriodictyol could therefore have the potential to treat
S. aureus
infection targeting Hla.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>29671126</pmid><doi>10.1007/s11274-018-2446-3</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0959-3993 |
| ispartof | World journal of microbiology & biotechnology, 2018-05, Vol.34 (5), p.64-7, Article 64 |
| issn | 0959-3993 1573-0972 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2027596266 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | A549 Cells - drug effects Anti-Bacterial Agents - pharmacology Antibiotics Applied Microbiology Assaying Bacterial Toxins - genetics Bacterial Toxins - metabolism Bacterial Toxins - toxicity Biochemistry Biomedical and Life Sciences Biotechnology Cell injury Cytotoxicity Down-Regulation - drug effects Drug resistance Environmental Engineering/Biotechnology Exotoxins Flavanones - pharmacology Gene Expression Regulation, Bacterial - drug effects Gram-positive bacteria Hemolysin Proteins - drug effects Hemolysin Proteins - genetics Hemolysin Proteins - metabolism Hemolysin Proteins - toxicity Hemolysis Humans Life Sciences Lung Injury - microbiology Lung Injury - prevention & control Lungs Methicillin Methicillin-Resistant Staphylococcus aureus - drug effects Methicillin-Resistant Staphylococcus aureus - metabolism Microbial Sensitivity Tests Microbiology Original Paper Pathogens Penicillin Pneumonia, Staphylococcal - microbiology Pneumonia, Staphylococcal - pathology Pneumonia, Staphylococcal - prevention & control Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - growth & development Staphylococcus aureus - metabolism Staphylococcus aureus - pathogenicity Toxicity Transcription Vegetables Virulence Virulence factors Virulence Factors - metabolism Western blotting |
| title | Eriodictyol protects against Staphylococcus aureus-induced lung cell injury by inhibiting alpha-hemolysin expression |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T20%3A02%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Eriodictyol%20protects%20against%20Staphylococcus%20aureus-induced%20lung%20cell%20injury%20by%20inhibiting%20alpha-hemolysin%20expression&rft.jtitle=World%20journal%20of%20microbiology%20&%20biotechnology&rft.au=Xuewen,%20He&rft.date=2018-05-01&rft.volume=34&rft.issue=5&rft.spage=64&rft.epage=7&rft.pages=64-7&rft.artnum=64&rft.issn=0959-3993&rft.eissn=1573-0972&rft_id=info:doi/10.1007/s11274-018-2446-3&rft_dat=%3Cproquest_cross%3E2027596266%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2026838364&rft_id=info:pmid/29671126&rfr_iscdi=true |