Detection and variability of the lethal yellowing group (16Sr IV) phytoplasmas in the Cedusa sp. (Hemiptera: Auchenorrhyncha: Derbidae) in Jamaica
Lethal yellowing (LY) group phytoplasma was detected in members of the Cedusa species of Derbids from Jamaica by nested PCR assay employing the rRNA primer pairs P1/P7 and LY16Sf/LY16Sr. A 1400‐bp product was obtained from 13/43 Derbids that were analysed. Restriction fragment length polymorphism an...
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| Veröffentlicht in: | Annals of applied biology 2006-08, Vol.149 (1), p.53-62 |
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| creator | Brown, S.E. Been, B.O. McLaughlin, W.A. |
| description | Lethal yellowing (LY) group phytoplasma was detected in members of the Cedusa species of Derbids from Jamaica by nested PCR assay employing the rRNA primer pairs P1/P7 and LY16Sf/LY16Sr. A 1400‐bp product was obtained from 13/43 Derbids that were analysed. Restriction fragment length polymorphism analysis of the nested PCR product revealed variations in 6/13 Derbids analysed, suggesting that the planthoppers could be infected with different strains of the LY group phytoplasma. Sequence analysis of the 16S rDNA gene determined that the percentage of similarity of the phytoplasma strains in the Derbids ranged from 97–98% to the phytoplasma strain found in coconuts in Florida (LYFL‐C2) and Jamaica (LYJ‐C8). The phytoplasma strains in the Derbids clustered together with the Jamaican and Florida coconut LY phytoplasma, as well as other characterised strains composing the LY phytoplasma (16Sr IV) group. |
| doi_str_mv | 10.1111/j.1744-7348.2006.00072.x |
| format | Article |
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The phytoplasma strains in the Derbids clustered together with the Jamaican and Florida coconut LY phytoplasma, as well as other characterised strains composing the LY phytoplasma (16Sr IV) group.</description><identifier>ISSN: 0003-4746</identifier><identifier>EISSN: 1744-7348</identifier><identifier>EISSN: 1744-1348</identifier><identifier>DOI: 10.1111/j.1744-7348.2006.00072.x</identifier><identifier>CODEN: AABIAV</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>16S ribosomal RNA ; Auchenorrhyncha ; Bacterial plant pathogens ; Biological and medical sciences ; Cedusa sp ; Derbidae ; Fundamental and applied biological sciences. Psychology ; Hemiptera ; lethal yellowing group ; Phytopathology. Animal pests. Plant and forest protection ; phytoplasma ; Protozoa. Invertebrates ; Records, symptoms, damages, economic importance, population surveys</subject><ispartof>Annals of applied biology, 2006-08, Vol.149 (1), p.53-62</ispartof><rights>2006 Association of Applied Biologists</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5262-b6fdf03e38a376b7bf3c41251b76b7bd79055773f29a75c6fb1ae3b67e0efa2a3</citedby><cites>FETCH-LOGICAL-c5262-b6fdf03e38a376b7bf3c41251b76b7bd79055773f29a75c6fb1ae3b67e0efa2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1744-7348.2006.00072.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1744-7348.2006.00072.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18010053$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Brown, S.E.</creatorcontrib><creatorcontrib>Been, B.O.</creatorcontrib><creatorcontrib>McLaughlin, W.A.</creatorcontrib><title>Detection and variability of the lethal yellowing group (16Sr IV) phytoplasmas in the Cedusa sp. (Hemiptera: Auchenorrhyncha: Derbidae) in Jamaica</title><title>Annals of applied biology</title><description>Lethal yellowing (LY) group phytoplasma was detected in members of the Cedusa species of Derbids from Jamaica by nested PCR assay employing the rRNA primer pairs P1/P7 and LY16Sf/LY16Sr. A 1400‐bp product was obtained from 13/43 Derbids that were analysed. Restriction fragment length polymorphism analysis of the nested PCR product revealed variations in 6/13 Derbids analysed, suggesting that the planthoppers could be infected with different strains of the LY group phytoplasma. Sequence analysis of the 16S rDNA gene determined that the percentage of similarity of the phytoplasma strains in the Derbids ranged from 97–98% to the phytoplasma strain found in coconuts in Florida (LYFL‐C2) and Jamaica (LYJ‐C8). The phytoplasma strains in the Derbids clustered together with the Jamaican and Florida coconut LY phytoplasma, as well as other characterised strains composing the LY phytoplasma (16Sr IV) group.</description><subject>16S ribosomal RNA</subject><subject>Auchenorrhyncha</subject><subject>Bacterial plant pathogens</subject><subject>Biological and medical sciences</subject><subject>Cedusa sp</subject><subject>Derbidae</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemiptera</subject><subject>lethal yellowing group</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>phytoplasma</subject><subject>Protozoa. Invertebrates</subject><subject>Records, symptoms, damages, economic importance, population surveys</subject><issn>0003-4746</issn><issn>1744-7348</issn><issn>1744-1348</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqNkdtu1DAQhiMEEkvhHXwDai8SfEjsXbjabqEtqjhTLq2JM2685FQ7oZvX4IlJdqtyi2-sGf_fP_L8UUQYTdh0Xm8TptI0ViJdJpxSmVBKFU92j6LFw8PjaDF1RZyqVD6NnoWwncoVXfFF9OcMezS9axsCTUF-g3eQu8r1I2kt6UskFfYlVGTEqmrvXHNDbnw7dOSYyW-eXF6fkK4c-7arINQQiGv20AaLIQAJXUKOL7B2XY8e3pD1YEpsWu_LsTHl1DhDn7sC8GQGP0ANzsDz6ImFKuCL-_so-vH-3ffNRXz16fxys76KTcYlj3NpC0sFiiUIJXOVW2FSxjOW76tCrWiWKSUsX4HKjLQ5AxS5VEjRAgdxFL06-Ha-vR0w9Lp2wUzfhAbbIWhOucpWTEzC5UFofBuCR6s772rwo2ZUzyHorZ53redd6zkEvQ9B7yb05f0MCAYq66ExLvzjl5RRms0j3h50d67C8b_99Xp9qvhExwfahR53DzT4X1oqoTL98-O5_iJPr7-qyeCz-AsJUank</recordid><startdate>200608</startdate><enddate>200608</enddate><creator>Brown, S.E.</creator><creator>Been, B.O.</creator><creator>McLaughlin, W.A.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7SS</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>200608</creationdate><title>Detection and variability of the lethal yellowing group (16Sr IV) phytoplasmas in the Cedusa sp. 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Invertebrates</topic><topic>Records, symptoms, damages, economic importance, population surveys</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brown, S.E.</creatorcontrib><creatorcontrib>Been, B.O.</creatorcontrib><creatorcontrib>McLaughlin, W.A.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Annals of applied biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brown, S.E.</au><au>Been, B.O.</au><au>McLaughlin, W.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection and variability of the lethal yellowing group (16Sr IV) phytoplasmas in the Cedusa sp. (Hemiptera: Auchenorrhyncha: Derbidae) in Jamaica</atitle><jtitle>Annals of applied biology</jtitle><date>2006-08</date><risdate>2006</risdate><volume>149</volume><issue>1</issue><spage>53</spage><epage>62</epage><pages>53-62</pages><issn>0003-4746</issn><eissn>1744-7348</eissn><eissn>1744-1348</eissn><coden>AABIAV</coden><abstract>Lethal yellowing (LY) group phytoplasma was detected in members of the Cedusa species of Derbids from Jamaica by nested PCR assay employing the rRNA primer pairs P1/P7 and LY16Sf/LY16Sr. A 1400‐bp product was obtained from 13/43 Derbids that were analysed. Restriction fragment length polymorphism analysis of the nested PCR product revealed variations in 6/13 Derbids analysed, suggesting that the planthoppers could be infected with different strains of the LY group phytoplasma. Sequence analysis of the 16S rDNA gene determined that the percentage of similarity of the phytoplasma strains in the Derbids ranged from 97–98% to the phytoplasma strain found in coconuts in Florida (LYFL‐C2) and Jamaica (LYJ‐C8). The phytoplasma strains in the Derbids clustered together with the Jamaican and Florida coconut LY phytoplasma, as well as other characterised strains composing the LY phytoplasma (16Sr IV) group.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1744-7348.2006.00072.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library All Journals |
| subjects | 16S ribosomal RNA Auchenorrhyncha Bacterial plant pathogens Biological and medical sciences Cedusa sp Derbidae Fundamental and applied biological sciences. Psychology Hemiptera lethal yellowing group Phytopathology. Animal pests. Plant and forest protection phytoplasma Protozoa. Invertebrates Records, symptoms, damages, economic importance, population surveys |
| title | Detection and variability of the lethal yellowing group (16Sr IV) phytoplasmas in the Cedusa sp. (Hemiptera: Auchenorrhyncha: Derbidae) in Jamaica |
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