Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates
Balantioides coli is a ciliated protozoon that inhabits the intestine of pigs, non-human primates and humans. Light microscopy studies have described over 50 species of the genus Balantioides but their validity is in doubt. Due to the limited information about this genus, this study is aimed to iden...
Gespeichert in:
| Veröffentlicht in: | Acta parasitologica 2018-06, Vol.63 (2), p.287-298 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 298 |
|---|---|
| container_issue | 2 |
| container_start_page | 287 |
| container_title | Acta parasitologica |
| container_volume | 63 |
| creator | da Barbosa, Alynne Silva Barbosa, Helene Santos de Souza, Sandra Maria Oliveira Dib, Laís Verdan Uchôa, Claudia Maria Antunes Bastos, Otilio Machado Pereira Amendoeira, Maria Regina Reis |
| description | Balantioides coli
is a ciliated protozoon that inhabits the intestine of pigs, non-human primates and humans. Light microscopy studies have described over 50 species of the genus
Balantioides
but their validity is in doubt. Due to the limited information about this genus, this study is aimed to identify morphological characteristics of
Balantioides coli
isolated using fluorescence microscopy and both scanning (SEM) and transmission electron microscopy (TEM). Trophozoites isolated from the feces of pig and macaque were washed and subjected to centrifugation. These cells were fixed with paraformaldehyde for immunofluorescence. Other aliquots of these trophozoites were fixed with glutaraldehyde, post fixed with osmium tetroxide and processed for SEM and TEM. Immunofluorescence studies revealed microtubules with a longitudinal distribution to the main axis of the parasite and in the constitution of cilia. SEM demonstrated a high concentration of cilia covering the oral apparatus and a poor presence of such structures in cytopyge. TEM revealed in the plasma membrane, several associated structures were observed to delineate the cellular cortex and mucocysts. The cytoskeleton of the oral region was observed in detail and had an organization pattern consisting of microtubules, which formed files and nematodesmal networks. Organelles such as hydrogenosomes like and peroxisomes were observed close to the cortex. Macronuclei were observed, but structures that were consistent with micronuclei were not identified. Ultrastructural morphological analysis of isolates confirms its similarity to
Balantioides coli
. In this study were identified structures that had not yet been described, such as hydrogenosomes like and cytoskeletal structures. |
| doi_str_mv | 10.1515/ap-2018-0033 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2025317911</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2038321952</sourcerecordid><originalsourceid>FETCH-LOGICAL-c408t-15cb09f0bc4de5d55ab4d1158cc77890d0c772e2fa2a25d829ccb90bbd168c553</originalsourceid><addsrcrecordid>eNqFkc1PGzEQxS0EgpT2xrlaiQuHGjx2vPHmBqgtSJG40PPKa3sTR9711h-q-O_rJJRKVSVOb2T95nlmHkIXQK6BA7-RE6YEBCaEsSM0A9HUGASH41JTRjAVFM7Qhxi3hMxrIcQpOqNNzUtdz9D2Tjo5JuutNrFS3tllNfgwbbzza6ukq-Soq-xSkDGFrFIO5U1tZJAqmWBjsipWvq8mu96jox_xJg9yrKZgB5lMZaN3ReNHdNJLF82nVz1HP759fb5_wKun74_3tyus5kQkDFx1pOlJp-bacM257OYagAulFgvREE2KUkN7SSXlWtBGqa4hXaehFopzdo6uDr5T8D-ziakdbFTGlT2Nz7GlhHIGiwagoJf_oFufw1imKxQTjELDaaG-HCgVfIzB9O1-tfDSAml3GbRyancZtLsMCv751TR3g9Fv8J-jF2B5AH5JV26ozTrkl1L8_fx_vjWjVCxKMz40x-I7rt_rYr8B_Oyk3Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2038321952</pqid></control><display><type>article</type><title>Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>da Barbosa, Alynne Silva ; Barbosa, Helene Santos ; de Souza, Sandra Maria Oliveira ; Dib, Laís Verdan ; Uchôa, Claudia Maria Antunes ; Bastos, Otilio Machado Pereira ; Amendoeira, Maria Regina Reis</creator><creatorcontrib>da Barbosa, Alynne Silva ; Barbosa, Helene Santos ; de Souza, Sandra Maria Oliveira ; Dib, Laís Verdan ; Uchôa, Claudia Maria Antunes ; Bastos, Otilio Machado Pereira ; Amendoeira, Maria Regina Reis</creatorcontrib><description>Balantioides coli
is a ciliated protozoon that inhabits the intestine of pigs, non-human primates and humans. Light microscopy studies have described over 50 species of the genus
Balantioides
but their validity is in doubt. Due to the limited information about this genus, this study is aimed to identify morphological characteristics of
Balantioides coli
isolated using fluorescence microscopy and both scanning (SEM) and transmission electron microscopy (TEM). Trophozoites isolated from the feces of pig and macaque were washed and subjected to centrifugation. These cells were fixed with paraformaldehyde for immunofluorescence. Other aliquots of these trophozoites were fixed with glutaraldehyde, post fixed with osmium tetroxide and processed for SEM and TEM. Immunofluorescence studies revealed microtubules with a longitudinal distribution to the main axis of the parasite and in the constitution of cilia. SEM demonstrated a high concentration of cilia covering the oral apparatus and a poor presence of such structures in cytopyge. TEM revealed in the plasma membrane, several associated structures were observed to delineate the cellular cortex and mucocysts. The cytoskeleton of the oral region was observed in detail and had an organization pattern consisting of microtubules, which formed files and nematodesmal networks. Organelles such as hydrogenosomes like and peroxisomes were observed close to the cortex. Macronuclei were observed, but structures that were consistent with micronuclei were not identified. Ultrastructural morphological analysis of isolates confirms its similarity to
Balantioides coli
. In this study were identified structures that had not yet been described, such as hydrogenosomes like and cytoskeletal structures.</description><identifier>ISSN: 1230-2821</identifier><identifier>EISSN: 1896-1851</identifier><identifier>DOI: 10.1515/ap-2018-0033</identifier><identifier>PMID: 29654686</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animal Systematics/Taxonomy/Biogeography ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Cell Membrane - ultrastructure ; Cellular structure ; Centrifugation ; Cilia ; ciliates ; Constitution ; Cytoskeleton ; Ecology ; Feces - parasitology ; Fluorescence ; Fluorescence microscopy ; Glutaraldehyde ; Humans ; Hydrogenosomes ; hydrogenosomes like ; Immunofluorescence ; Intestine ; Intestines - parasitology ; Light microscopy ; Macronuclei ; Medical Microbiology ; Microbiology ; Micronuclei ; Microscopy ; Microscopy, Electron, Scanning - methods ; Microscopy, Electron, Transmission - methods ; Microtubules ; Microtubules - ultrastructure ; Morphology ; Oral apparatus ; Organelles ; Organelles - ultrastructure ; Osmium ; Osmium tetroxide ; Parasites - anatomy & histology ; Parasites - isolation & purification ; Parasites - ultrastructure ; Parasitology ; Peroxisomes ; Peroxisomes - ultrastructure ; Physical characteristics ; Primates ; Primates - parasitology ; Protozoan Infections, Animal - parasitology ; Scanning electron microscopy ; SEM ; Swine - parasitology ; TEM ; Transmission electron microscopy ; Trophozoites ; Trophozoites - isolation & purification ; Trophozoites - ultrastructure</subject><ispartof>Acta parasitologica, 2018-06, Vol.63 (2), p.287-298</ispartof><rights>Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2018</rights><rights>Copyright Walter de Gruyter GmbH 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-15cb09f0bc4de5d55ab4d1158cc77890d0c772e2fa2a25d829ccb90bbd168c553</citedby><cites>FETCH-LOGICAL-c408t-15cb09f0bc4de5d55ab4d1158cc77890d0c772e2fa2a25d829ccb90bbd168c553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1515/ap-2018-0033$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1515/ap-2018-0033$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29654686$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>da Barbosa, Alynne Silva</creatorcontrib><creatorcontrib>Barbosa, Helene Santos</creatorcontrib><creatorcontrib>de Souza, Sandra Maria Oliveira</creatorcontrib><creatorcontrib>Dib, Laís Verdan</creatorcontrib><creatorcontrib>Uchôa, Claudia Maria Antunes</creatorcontrib><creatorcontrib>Bastos, Otilio Machado Pereira</creatorcontrib><creatorcontrib>Amendoeira, Maria Regina Reis</creatorcontrib><title>Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates</title><title>Acta parasitologica</title><addtitle>Acta Parasit</addtitle><addtitle>Acta Parasitol</addtitle><description>Balantioides coli
is a ciliated protozoon that inhabits the intestine of pigs, non-human primates and humans. Light microscopy studies have described over 50 species of the genus
Balantioides
but their validity is in doubt. Due to the limited information about this genus, this study is aimed to identify morphological characteristics of
Balantioides coli
isolated using fluorescence microscopy and both scanning (SEM) and transmission electron microscopy (TEM). Trophozoites isolated from the feces of pig and macaque were washed and subjected to centrifugation. These cells were fixed with paraformaldehyde for immunofluorescence. Other aliquots of these trophozoites were fixed with glutaraldehyde, post fixed with osmium tetroxide and processed for SEM and TEM. Immunofluorescence studies revealed microtubules with a longitudinal distribution to the main axis of the parasite and in the constitution of cilia. SEM demonstrated a high concentration of cilia covering the oral apparatus and a poor presence of such structures in cytopyge. TEM revealed in the plasma membrane, several associated structures were observed to delineate the cellular cortex and mucocysts. The cytoskeleton of the oral region was observed in detail and had an organization pattern consisting of microtubules, which formed files and nematodesmal networks. Organelles such as hydrogenosomes like and peroxisomes were observed close to the cortex. Macronuclei were observed, but structures that were consistent with micronuclei were not identified. Ultrastructural morphological analysis of isolates confirms its similarity to
Balantioides coli
. In this study were identified structures that had not yet been described, such as hydrogenosomes like and cytoskeletal structures.</description><subject>Animal Systematics/Taxonomy/Biogeography</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cellular structure</subject><subject>Centrifugation</subject><subject>Cilia</subject><subject>ciliates</subject><subject>Constitution</subject><subject>Cytoskeleton</subject><subject>Ecology</subject><subject>Feces - parasitology</subject><subject>Fluorescence</subject><subject>Fluorescence microscopy</subject><subject>Glutaraldehyde</subject><subject>Humans</subject><subject>Hydrogenosomes</subject><subject>hydrogenosomes like</subject><subject>Immunofluorescence</subject><subject>Intestine</subject><subject>Intestines - parasitology</subject><subject>Light microscopy</subject><subject>Macronuclei</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Micronuclei</subject><subject>Microscopy</subject><subject>Microscopy, Electron, Scanning - methods</subject><subject>Microscopy, Electron, Transmission - methods</subject><subject>Microtubules</subject><subject>Microtubules - ultrastructure</subject><subject>Morphology</subject><subject>Oral apparatus</subject><subject>Organelles</subject><subject>Organelles - ultrastructure</subject><subject>Osmium</subject><subject>Osmium tetroxide</subject><subject>Parasites - anatomy & histology</subject><subject>Parasites - isolation & purification</subject><subject>Parasites - ultrastructure</subject><subject>Parasitology</subject><subject>Peroxisomes</subject><subject>Peroxisomes - ultrastructure</subject><subject>Physical characteristics</subject><subject>Primates</subject><subject>Primates - parasitology</subject><subject>Protozoan Infections, Animal - parasitology</subject><subject>Scanning electron microscopy</subject><subject>SEM</subject><subject>Swine - parasitology</subject><subject>TEM</subject><subject>Transmission electron microscopy</subject><subject>Trophozoites</subject><subject>Trophozoites - isolation & purification</subject><subject>Trophozoites - ultrastructure</subject><issn>1230-2821</issn><issn>1896-1851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkc1PGzEQxS0EgpT2xrlaiQuHGjx2vPHmBqgtSJG40PPKa3sTR9711h-q-O_rJJRKVSVOb2T95nlmHkIXQK6BA7-RE6YEBCaEsSM0A9HUGASH41JTRjAVFM7Qhxi3hMxrIcQpOqNNzUtdz9D2Tjo5JuutNrFS3tllNfgwbbzza6ukq-Soq-xSkDGFrFIO5U1tZJAqmWBjsipWvq8mu96jox_xJg9yrKZgB5lMZaN3ReNHdNJLF82nVz1HP759fb5_wKun74_3tyus5kQkDFx1pOlJp-bacM257OYagAulFgvREE2KUkN7SSXlWtBGqa4hXaehFopzdo6uDr5T8D-ziakdbFTGlT2Nz7GlhHIGiwagoJf_oFufw1imKxQTjELDaaG-HCgVfIzB9O1-tfDSAml3GbRyancZtLsMCv751TR3g9Fv8J-jF2B5AH5JV26ozTrkl1L8_fx_vjWjVCxKMz40x-I7rt_rYr8B_Oyk3Q</recordid><startdate>20180626</startdate><enddate>20180626</enddate><creator>da Barbosa, Alynne Silva</creator><creator>Barbosa, Helene Santos</creator><creator>de Souza, Sandra Maria Oliveira</creator><creator>Dib, Laís Verdan</creator><creator>Uchôa, Claudia Maria Antunes</creator><creator>Bastos, Otilio Machado Pereira</creator><creator>Amendoeira, Maria Regina Reis</creator><general>Springer International Publishing</general><general>De Gruyter</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20180626</creationdate><title>Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates</title><author>da Barbosa, Alynne Silva ; Barbosa, Helene Santos ; de Souza, Sandra Maria Oliveira ; Dib, Laís Verdan ; Uchôa, Claudia Maria Antunes ; Bastos, Otilio Machado Pereira ; Amendoeira, Maria Regina Reis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-15cb09f0bc4de5d55ab4d1158cc77890d0c772e2fa2a25d829ccb90bbd168c553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animal Systematics/Taxonomy/Biogeography</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Membrane - ultrastructure</topic><topic>Cellular structure</topic><topic>Centrifugation</topic><topic>Cilia</topic><topic>ciliates</topic><topic>Constitution</topic><topic>Cytoskeleton</topic><topic>Ecology</topic><topic>Feces - parasitology</topic><topic>Fluorescence</topic><topic>Fluorescence microscopy</topic><topic>Glutaraldehyde</topic><topic>Humans</topic><topic>Hydrogenosomes</topic><topic>hydrogenosomes like</topic><topic>Immunofluorescence</topic><topic>Intestine</topic><topic>Intestines - parasitology</topic><topic>Light microscopy</topic><topic>Macronuclei</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Micronuclei</topic><topic>Microscopy</topic><topic>Microscopy, Electron, Scanning - methods</topic><topic>Microscopy, Electron, Transmission - methods</topic><topic>Microtubules</topic><topic>Microtubules - ultrastructure</topic><topic>Morphology</topic><topic>Oral apparatus</topic><topic>Organelles</topic><topic>Organelles - ultrastructure</topic><topic>Osmium</topic><topic>Osmium tetroxide</topic><topic>Parasites - anatomy & histology</topic><topic>Parasites - isolation & purification</topic><topic>Parasites - ultrastructure</topic><topic>Parasitology</topic><topic>Peroxisomes</topic><topic>Peroxisomes - ultrastructure</topic><topic>Physical characteristics</topic><topic>Primates</topic><topic>Primates - parasitology</topic><topic>Protozoan Infections, Animal - parasitology</topic><topic>Scanning electron microscopy</topic><topic>SEM</topic><topic>Swine - parasitology</topic><topic>TEM</topic><topic>Transmission electron microscopy</topic><topic>Trophozoites</topic><topic>Trophozoites - isolation & purification</topic><topic>Trophozoites - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>da Barbosa, Alynne Silva</creatorcontrib><creatorcontrib>Barbosa, Helene Santos</creatorcontrib><creatorcontrib>de Souza, Sandra Maria Oliveira</creatorcontrib><creatorcontrib>Dib, Laís Verdan</creatorcontrib><creatorcontrib>Uchôa, Claudia Maria Antunes</creatorcontrib><creatorcontrib>Bastos, Otilio Machado Pereira</creatorcontrib><creatorcontrib>Amendoeira, Maria Regina Reis</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Acta parasitologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>da Barbosa, Alynne Silva</au><au>Barbosa, Helene Santos</au><au>de Souza, Sandra Maria Oliveira</au><au>Dib, Laís Verdan</au><au>Uchôa, Claudia Maria Antunes</au><au>Bastos, Otilio Machado Pereira</au><au>Amendoeira, Maria Regina Reis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates</atitle><jtitle>Acta parasitologica</jtitle><stitle>Acta Parasit</stitle><addtitle>Acta Parasitol</addtitle><date>2018-06-26</date><risdate>2018</risdate><volume>63</volume><issue>2</issue><spage>287</spage><epage>298</epage><pages>287-298</pages><issn>1230-2821</issn><eissn>1896-1851</eissn><abstract>Balantioides coli
is a ciliated protozoon that inhabits the intestine of pigs, non-human primates and humans. Light microscopy studies have described over 50 species of the genus
Balantioides
but their validity is in doubt. Due to the limited information about this genus, this study is aimed to identify morphological characteristics of
Balantioides coli
isolated using fluorescence microscopy and both scanning (SEM) and transmission electron microscopy (TEM). Trophozoites isolated from the feces of pig and macaque were washed and subjected to centrifugation. These cells were fixed with paraformaldehyde for immunofluorescence. Other aliquots of these trophozoites were fixed with glutaraldehyde, post fixed with osmium tetroxide and processed for SEM and TEM. Immunofluorescence studies revealed microtubules with a longitudinal distribution to the main axis of the parasite and in the constitution of cilia. SEM demonstrated a high concentration of cilia covering the oral apparatus and a poor presence of such structures in cytopyge. TEM revealed in the plasma membrane, several associated structures were observed to delineate the cellular cortex and mucocysts. The cytoskeleton of the oral region was observed in detail and had an organization pattern consisting of microtubules, which formed files and nematodesmal networks. Organelles such as hydrogenosomes like and peroxisomes were observed close to the cortex. Macronuclei were observed, but structures that were consistent with micronuclei were not identified. Ultrastructural morphological analysis of isolates confirms its similarity to
Balantioides coli
. In this study were identified structures that had not yet been described, such as hydrogenosomes like and cytoskeletal structures.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>29654686</pmid><doi>10.1515/ap-2018-0033</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1230-2821 |
| ispartof | Acta parasitologica, 2018-06, Vol.63 (2), p.287-298 |
| issn | 1230-2821 1896-1851 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2025317911 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animal Systematics/Taxonomy/Biogeography Animals Biomedical and Life Sciences Biomedicine Cell Membrane - ultrastructure Cellular structure Centrifugation Cilia ciliates Constitution Cytoskeleton Ecology Feces - parasitology Fluorescence Fluorescence microscopy Glutaraldehyde Humans Hydrogenosomes hydrogenosomes like Immunofluorescence Intestine Intestines - parasitology Light microscopy Macronuclei Medical Microbiology Microbiology Micronuclei Microscopy Microscopy, Electron, Scanning - methods Microscopy, Electron, Transmission - methods Microtubules Microtubules - ultrastructure Morphology Oral apparatus Organelles Organelles - ultrastructure Osmium Osmium tetroxide Parasites - anatomy & histology Parasites - isolation & purification Parasites - ultrastructure Parasitology Peroxisomes Peroxisomes - ultrastructure Physical characteristics Primates Primates - parasitology Protozoan Infections, Animal - parasitology Scanning electron microscopy SEM Swine - parasitology TEM Transmission electron microscopy Trophozoites Trophozoites - isolation & purification Trophozoites - ultrastructure |
| title | Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T20%3A34%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Balantioides%20coli:%20morphological%20and%20ultrastructural%20characteristics%20of%20pig%20and%20non-human%20primate%20isolates&rft.jtitle=Acta%20parasitologica&rft.au=da%20Barbosa,%20Alynne%20Silva&rft.date=2018-06-26&rft.volume=63&rft.issue=2&rft.spage=287&rft.epage=298&rft.pages=287-298&rft.issn=1230-2821&rft.eissn=1896-1851&rft_id=info:doi/10.1515/ap-2018-0033&rft_dat=%3Cproquest_cross%3E2038321952%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2038321952&rft_id=info:pmid/29654686&rfr_iscdi=true |