Glucocorticoid pretreatment increases toxicity due to peroxides in alveolar epithelial-like cell lines
Abstract In previous experiments an increase in zinc-mediated toxicity was found after pretreatment of alveolar epithelial type II-like cells with glucocorticoids. In this work toxicity of two peroxides (tertiary butyl hydroperoxide [tBHP], hydrogene peroxide [HP]) was assessed in L2 and A549 cells...
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| Veröffentlicht in: | Toxicology (Amsterdam) 2009-02, Vol.256 (1), p.48-52 |
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| creator | Walther, Udo I Stets, Regine |
| description | Abstract In previous experiments an increase in zinc-mediated toxicity was found after pretreatment of alveolar epithelial type II-like cells with glucocorticoids. In this work toxicity of two peroxides (tertiary butyl hydroperoxide [tBHP], hydrogene peroxide [HP]) was assessed in L2 and A549 cells compared to dexamethasone (DEX) pretreated cells. Pretreatment of cells with 7.5 μmol/l DEX for 72 h decreased cellular glutathione content in both cell lines. Furthermore compared to not pretreated cells toxicity of both peroxides was increased in A549 cells, while in L2 cells only toxicity of tBHP was significantly increased by the glucocorticoid pretreatment. HP toxicity only showed a tendency to be increased in L2 cells after DEX pretreatment. The results point to a glucocorticoid-dependent increased oxidative stress of alveolar epithelial type II cells as antagonised by antioxidative enzymes such as catalase and/or preferentially by the glutathione system. This furthermore should be considered for all glucocorticoid applications in vivo as well. |
| doi_str_mv | 10.1016/j.tox.2008.11.001 |
| format | Article |
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In this work toxicity of two peroxides (tertiary butyl hydroperoxide [tBHP], hydrogene peroxide [HP]) was assessed in L2 and A549 cells compared to dexamethasone (DEX) pretreated cells. Pretreatment of cells with 7.5 μmol/l DEX for 72 h decreased cellular glutathione content in both cell lines. Furthermore compared to not pretreated cells toxicity of both peroxides was increased in A549 cells, while in L2 cells only toxicity of tBHP was significantly increased by the glucocorticoid pretreatment. HP toxicity only showed a tendency to be increased in L2 cells after DEX pretreatment. The results point to a glucocorticoid-dependent increased oxidative stress of alveolar epithelial type II cells as antagonised by antioxidative enzymes such as catalase and/or preferentially by the glutathione system. 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This furthermore should be considered for all glucocorticoid applications in vivo as well.</description><subject>A549 cells</subject><subject>Algorithms</subject><subject>Biological and medical sciences</subject><subject>Butyl hydroperoxide</subject><subject>Dexamethasone</subject><subject>Dexamethasone - pharmacology</subject><subject>Emergency</subject><subject>Epithelial Cells - drug effects</subject><subject>Glucocorticoids - pharmacology</subject><subject>Glutathione - metabolism</subject><subject>Glutathione Reductase - metabolism</subject><subject>Humans</subject><subject>Hydrogene peroxide</subject><subject>L2 cells</subject><subject>Medical sciences</subject><subject>Oxidative Stress - drug effects</subject><subject>Peroxides - toxicity</subject><subject>Protein Synthesis Inhibitors - pharmacology</subject><subject>Pulmonary Alveoli - cytology</subject><subject>Pulmonary Alveoli - drug effects</subject><subject>tert-Butylhydroperoxide - toxicity</subject><subject>Toxicology</subject><issn>0300-483X</issn><issn>1879-3185</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kk9v1DAQxS0EokvpB-CCcqG3hJkkdhwhIaGqtEiVOABSb5bXmQhvvXGwnYr99jjaFZU4cPK_92aefxrG3iBUCCje76rkf1c1gKwQKwB8xjYou75sUPLnbAMNQNnK5v6MvYpxBwB104qX7Ax74KLl3YaNN24x3viQrPF2KOZAKZBOe5pSYSeT95FikftYY9OhGBbKh2KmkG-G_GKnQrtH8k6HgmabfpKz2pXOPlBhyLnC2Ynia_Zi1C7SxWk9Zz8-X3-_ui3vvt58ufp0V5pWdKnUW6SRt2PPm1GS7Htd85aE3I6iQT1sDReCsO7REMihRaGl0ZI6UfPBZEtzzi6Pdefgfy0Uk9rbuMbQE_klqhrqzKPjWYhHoQk-xkCjmoPd63BQCGqFq3Yqf1qtcBWiynCz5-2p-LLd0_DkONHMgncngY5GuzHoydj4V1cj9NiLVffhqKOM4tFSUNFYmgwNNpBJavD2vzE-_uM2mbHNDR_oQHHnlzBlxgpVrBWob-sUrEMAEoDnDM0f1GyutA</recordid><startdate>20090204</startdate><enddate>20090204</enddate><creator>Walther, Udo I</creator><creator>Stets, Regine</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20090204</creationdate><title>Glucocorticoid pretreatment increases toxicity due to peroxides in alveolar epithelial-like cell lines</title><author>Walther, Udo I ; Stets, Regine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-ab1ef54f953f8e899a254e68bf631adbc566e1291ce08d416a8ca8e7625dc3f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>A549 cells</topic><topic>Algorithms</topic><topic>Biological and medical sciences</topic><topic>Butyl hydroperoxide</topic><topic>Dexamethasone</topic><topic>Dexamethasone - pharmacology</topic><topic>Emergency</topic><topic>Epithelial Cells - drug effects</topic><topic>Glucocorticoids - pharmacology</topic><topic>Glutathione - metabolism</topic><topic>Glutathione Reductase - metabolism</topic><topic>Humans</topic><topic>Hydrogene peroxide</topic><topic>L2 cells</topic><topic>Medical sciences</topic><topic>Oxidative Stress - drug effects</topic><topic>Peroxides - toxicity</topic><topic>Protein Synthesis Inhibitors - pharmacology</topic><topic>Pulmonary Alveoli - cytology</topic><topic>Pulmonary Alveoli - drug effects</topic><topic>tert-Butylhydroperoxide - toxicity</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Walther, Udo I</creatorcontrib><creatorcontrib>Stets, Regine</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology (Amsterdam)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Walther, Udo I</au><au>Stets, Regine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glucocorticoid pretreatment increases toxicity due to peroxides in alveolar epithelial-like cell lines</atitle><jtitle>Toxicology (Amsterdam)</jtitle><addtitle>Toxicology</addtitle><date>2009-02-04</date><risdate>2009</risdate><volume>256</volume><issue>1</issue><spage>48</spage><epage>52</epage><pages>48-52</pages><issn>0300-483X</issn><eissn>1879-3185</eissn><coden>TXICDD</coden><abstract>Abstract In previous experiments an increase in zinc-mediated toxicity was found after pretreatment of alveolar epithelial type II-like cells with glucocorticoids. 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| subjects | A549 cells Algorithms Biological and medical sciences Butyl hydroperoxide Dexamethasone Dexamethasone - pharmacology Emergency Epithelial Cells - drug effects Glucocorticoids - pharmacology Glutathione - metabolism Glutathione Reductase - metabolism Humans Hydrogene peroxide L2 cells Medical sciences Oxidative Stress - drug effects Peroxides - toxicity Protein Synthesis Inhibitors - pharmacology Pulmonary Alveoli - cytology Pulmonary Alveoli - drug effects tert-Butylhydroperoxide - toxicity Toxicology |
| title | Glucocorticoid pretreatment increases toxicity due to peroxides in alveolar epithelial-like cell lines |
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