Assessment of genetic diversity among some Indian wheat cultivars using random amplified polymorphic DNA (RAPD) markers

Assessment of genetic diversity among some Indian wheat cultivars using random amplified polymorphic DNA (RAPD) markers

Genetic relationship study was performed with RAPD primers among 20 Indian wheat accessions (17 hexaploids, 2 tetraploids and 1 diploid). A total of 372 bands were observed using 25 RAPD primers, of which 323 (86.8%) were polymorphic. The total number of amplification products per primer ranged from...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Indian journal of biotechnology 2007-01, Vol.6 (1), p.18-23
Hauptverfasser: Grewal, S, Kharb, P, Malik, R, Jain, S, Jain, R K
Format: Artikel
Sprache:eng
Schlagworte:
Triticum aestivum
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 23
container_issue 1
container_start_page 18
container_title Indian journal of biotechnology
container_volume 6
creator Grewal, S
Kharb, P
Malik, R
Jain, S
Jain, R K
description Genetic relationship study was performed with RAPD primers among 20 Indian wheat accessions (17 hexaploids, 2 tetraploids and 1 diploid). A total of 372 bands were observed using 25 RAPD primers, of which 323 (86.8%) were polymorphic. The total number of amplification products per primer ranged from 5 (OPO-02) to 30 (OPH-01) with an average of 14.8 plus or minus 1.15 bands per primer. The size of PCR products ranged from 162 bp to 2529 bp. A significant corelation (0.933, p
format Article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_20241931</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20241931</sourcerecordid><originalsourceid>FETCH-LOGICAL-p101t-23042098b62f87c2a689ed31e04d04d5c6d46719bcfcfeea74e4c09f1fc748ab3</originalsourceid><addsrcrecordid>eNotzM1KAzEUhuFZKFir95CV6GIgyWR-shxafwpFRXRd0uSkjU6ScU6mpXffAYUPvs3Lc5HNqKx5XjZCXmXXiN-UVqVsqll2bBEB0UNIJFqygwDJaWLcAQZ06USUj2FHMHogq2CcCuS4B5WIHrvkDmpAMqKbikEFE_2U952zDgzpY3fycej3E7d8bcn9R_u-fCBeDT8TfZNdWtUh3P7_PPt6evxcvOTrt-fVol3nPaMs5bygglPZbCtum1pzVTUSTMGACjOt1JURVc3kVlttAVQtQGgqLbO6Fo3aFvPs7s_th_g7AqaNd6ih61SAOOKGUy6YLFhxBjEmWo8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20241931</pqid></control><display><type>article</type><title>Assessment of genetic diversity among some Indian wheat cultivars using random amplified polymorphic DNA (RAPD) markers</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Grewal, S ; Kharb, P ; Malik, R ; Jain, S ; Jain, R K</creator><creatorcontrib>Grewal, S ; Kharb, P ; Malik, R ; Jain, S ; Jain, R K</creatorcontrib><description>Genetic relationship study was performed with RAPD primers among 20 Indian wheat accessions (17 hexaploids, 2 tetraploids and 1 diploid). A total of 372 bands were observed using 25 RAPD primers, of which 323 (86.8%) were polymorphic. The total number of amplification products per primer ranged from 5 (OPO-02) to 30 (OPH-01) with an average of 14.8 plus or minus 1.15 bands per primer. The size of PCR products ranged from 162 bp to 2529 bp. A significant corelation (0.933, p&lt;0.01) was observed between the total number of bands and the number of polymorphic bands. Sixteen primers amplified 30 unique bands for specific genotypes that would prove to be highly useful in identification of genotype and designing future breeding strategy. The similarity coefficient values ranged from 0.52 to 0.82, indicating high genetic variability among the wheat genotypes. The 20 wheat genotypes were grouped into two major clusters. Cluster I had the diploid genotype T. urartu, tetraploid variety WH 896 and hexaploid variety PBW 175. Rest of the 16 hexaploid varieties and a tetraploid variety (Khapli) were placed in the other major cluster.</description><identifier>ISSN: 0972-5849</identifier><language>eng</language><subject>Triticum aestivum</subject><ispartof>Indian journal of biotechnology, 2007-01, Vol.6 (1), p.18-23</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Grewal, S</creatorcontrib><creatorcontrib>Kharb, P</creatorcontrib><creatorcontrib>Malik, R</creatorcontrib><creatorcontrib>Jain, S</creatorcontrib><creatorcontrib>Jain, R K</creatorcontrib><title>Assessment of genetic diversity among some Indian wheat cultivars using random amplified polymorphic DNA (RAPD) markers</title><title>Indian journal of biotechnology</title><description>Genetic relationship study was performed with RAPD primers among 20 Indian wheat accessions (17 hexaploids, 2 tetraploids and 1 diploid). A total of 372 bands were observed using 25 RAPD primers, of which 323 (86.8%) were polymorphic. The total number of amplification products per primer ranged from 5 (OPO-02) to 30 (OPH-01) with an average of 14.8 plus or minus 1.15 bands per primer. The size of PCR products ranged from 162 bp to 2529 bp. A significant corelation (0.933, p&lt;0.01) was observed between the total number of bands and the number of polymorphic bands. Sixteen primers amplified 30 unique bands for specific genotypes that would prove to be highly useful in identification of genotype and designing future breeding strategy. The similarity coefficient values ranged from 0.52 to 0.82, indicating high genetic variability among the wheat genotypes. The 20 wheat genotypes were grouped into two major clusters. Cluster I had the diploid genotype T. urartu, tetraploid variety WH 896 and hexaploid variety PBW 175. Rest of the 16 hexaploid varieties and a tetraploid variety (Khapli) were placed in the other major cluster.</description><subject>Triticum aestivum</subject><issn>0972-5849</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNotzM1KAzEUhuFZKFir95CV6GIgyWR-shxafwpFRXRd0uSkjU6ScU6mpXffAYUPvs3Lc5HNqKx5XjZCXmXXiN-UVqVsqll2bBEB0UNIJFqygwDJaWLcAQZ06USUj2FHMHogq2CcCuS4B5WIHrvkDmpAMqKbikEFE_2U952zDgzpY3fycej3E7d8bcn9R_u-fCBeDT8TfZNdWtUh3P7_PPt6evxcvOTrt-fVol3nPaMs5bygglPZbCtum1pzVTUSTMGACjOt1JURVc3kVlttAVQtQGgqLbO6Fo3aFvPs7s_th_g7AqaNd6ih61SAOOKGUy6YLFhxBjEmWo8</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>Grewal, S</creator><creator>Kharb, P</creator><creator>Malik, R</creator><creator>Jain, S</creator><creator>Jain, R K</creator><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20070101</creationdate><title>Assessment of genetic diversity among some Indian wheat cultivars using random amplified polymorphic DNA (RAPD) markers</title><author>Grewal, S ; Kharb, P ; Malik, R ; Jain, S ; Jain, R K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p101t-23042098b62f87c2a689ed31e04d04d5c6d46719bcfcfeea74e4c09f1fc748ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Triticum aestivum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grewal, S</creatorcontrib><creatorcontrib>Kharb, P</creatorcontrib><creatorcontrib>Malik, R</creatorcontrib><creatorcontrib>Jain, S</creatorcontrib><creatorcontrib>Jain, R K</creatorcontrib><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Indian journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grewal, S</au><au>Kharb, P</au><au>Malik, R</au><au>Jain, S</au><au>Jain, R K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of genetic diversity among some Indian wheat cultivars using random amplified polymorphic DNA (RAPD) markers</atitle><jtitle>Indian journal of biotechnology</jtitle><date>2007-01-01</date><risdate>2007</risdate><volume>6</volume><issue>1</issue><spage>18</spage><epage>23</epage><pages>18-23</pages><issn>0972-5849</issn><abstract>Genetic relationship study was performed with RAPD primers among 20 Indian wheat accessions (17 hexaploids, 2 tetraploids and 1 diploid). A total of 372 bands were observed using 25 RAPD primers, of which 323 (86.8%) were polymorphic. The total number of amplification products per primer ranged from 5 (OPO-02) to 30 (OPH-01) with an average of 14.8 plus or minus 1.15 bands per primer. The size of PCR products ranged from 162 bp to 2529 bp. A significant corelation (0.933, p&lt;0.01) was observed between the total number of bands and the number of polymorphic bands. Sixteen primers amplified 30 unique bands for specific genotypes that would prove to be highly useful in identification of genotype and designing future breeding strategy. The similarity coefficient values ranged from 0.52 to 0.82, indicating high genetic variability among the wheat genotypes. The 20 wheat genotypes were grouped into two major clusters. Cluster I had the diploid genotype T. urartu, tetraploid variety WH 896 and hexaploid variety PBW 175. Rest of the 16 hexaploid varieties and a tetraploid variety (Khapli) were placed in the other major cluster.</abstract><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0972-5849
ispartof Indian journal of biotechnology, 2007-01, Vol.6 (1), p.18-23
issn 0972-5849
language eng
recordid cdi_proquest_miscellaneous_20241931
source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
subjects Triticum aestivum
title Assessment of genetic diversity among some Indian wheat cultivars using random amplified polymorphic DNA (RAPD) markers
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T00%3A07%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Assessment%20of%20genetic%20diversity%20among%20some%20Indian%20wheat%20cultivars%20using%20random%20amplified%20polymorphic%20DNA%20(RAPD)%20markers&rft.jtitle=Indian%20journal%20of%20biotechnology&rft.au=Grewal,%20S&rft.date=2007-01-01&rft.volume=6&rft.issue=1&rft.spage=18&rft.epage=23&rft.pages=18-23&rft.issn=0972-5849&rft_id=info:doi/&rft_dat=%3Cproquest%3E20241931%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20241931&rft_id=info:pmid/&rfr_iscdi=true