Growth, Sporulation, δ-Endotoxins Synthesis, and Toxicity During Culture of Bacillus thuringiensis H14
Growth, sporulation, synthesis of δd-endotoxins, and toxicity against the larvae of Aedes aegypti and Culex pipiens were studied during fermentation of Bacillus thuringiensis H14 in a 20-L fermentor. Measurements of optical density and dielectric permittivity for biomass determination suggest a high...
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| Veröffentlicht in: | Current microbiology 2005-08, Vol.51 (2), p.75-81 |
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| creator | Sarrafzadeh, M.H Guiraud, J.P Lagneau, C Gaven, B Carron, A Navarro, J.M |
| description | Growth, sporulation, synthesis of δd-endotoxins, and toxicity against the larvae of Aedes aegypti and Culex pipiens were studied during fermentation of Bacillus thuringiensis H14 in a 20-L fermentor. Measurements of optical density and dielectric permittivity for biomass determination suggest a highly promising technique for on-line evaluation of sporulation. The synthesis of 65-, 25- and 130-kDa proteins started at 16, 18, and 23 h, respectively. These proteins were enriched in different ways until the end of culture (48 h). Toxicity in the course of sporulation was significantly different for the larvae of both mosquito species. Maximal activity against Ae. aegypti was obtained at the end of culture, whereas for Cx. pipiens, the sample at 38 h was the most active. |
| doi_str_mv | 10.1007/s00284-005-4463-3 |
| format | Article |
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Measurements of optical density and dielectric permittivity for biomass determination suggest a highly promising technique for on-line evaluation of sporulation. The synthesis of 65-, 25- and 130-kDa proteins started at 16, 18, and 23 h, respectively. These proteins were enriched in different ways until the end of culture (48 h). Toxicity in the course of sporulation was significantly different for the larvae of both mosquito species. 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Measurements of optical density and dielectric permittivity for biomass determination suggest a highly promising technique for on-line evaluation of sporulation. The synthesis of 65-, 25- and 130-kDa proteins started at 16, 18, and 23 h, respectively. These proteins were enriched in different ways until the end of culture (48 h). Toxicity in the course of sporulation was significantly different for the larvae of both mosquito species. Maximal activity against Ae. aegypti was obtained at the end of culture, whereas for Cx. pipiens, the sample at 38 h was the most active.</description><subject>Aedes - microbiology</subject><subject>Aedes aegypti</subject><subject>Animals</subject><subject>Bacillus thuringiensis</subject><subject>Bacillus thuringiensis - growth & development</subject><subject>Bacillus thuringiensis - metabolism</subject><subject>Bacillus thuringiensis - physiology</subject><subject>bacterial insecticides</subject><subject>Bacterial Proteins - metabolism</subject><subject>bacterial spores</subject><subject>bacterial toxins</subject><subject>Bacterial Toxins - metabolism</subject><subject>biological control</subject><subject>biological control agents</subject><subject>biosynthesis</subject><subject>crystal proteins</subject><subject>Culex</subject><subject>Culex pipiens</subject><subject>delta-endotoxins</subject><subject>Endotoxins - metabolism</subject><subject>entomopathogenic bacteria</subject><subject>Gram-positive bacteria</subject><subject>Hemolysin Proteins</subject><subject>larvae</subject><subject>mosquito control</subject><subject>Mosquito Control - methods</subject><subject>Spores, Bacterial</subject><subject>sporulation</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtuGzEMhoWiQeMmPUA3jVZdWQ0paR5aNm4eBQJk4WQtzINjqxiPHEmD1PfKOXqmjmMDAQiQIL-fi4-xrwg_EKC4jACy1AIgE1rnSqgPbIZaSQHG4Ec2A6WVKPMMT9nnGP8AoDSAn9gp5pCZopAztroN_iWt53y59WHsq-T8MOf_XsX10Prk_7oh8uVuSGuKLs55NbT8cdo2Lu34rzG4YcUXY5_GQNx3_KpqXN-Pkaf1283RMMX4HepzdtJVfaQvx37Gnm6uHxd34v7h9vfi571oVKaTqKmWBtuiblWtldKmMzVB1pqGkLREOc0Nla3MiizPSdVFUU7VUFsSYWfUGft--LsN_nmkmOzGxYb6vhrIj9FKkCovUU0gHsAm-BgDdXYb3KYKO4tg93btwa6d7Nq9XbvPfDs-H-sNte-Jo84JuDgAXeVttQou2qelBFSAYCAvQP0HjMmACg</recordid><startdate>20050801</startdate><enddate>20050801</enddate><creator>Sarrafzadeh, M.H</creator><creator>Guiraud, J.P</creator><creator>Lagneau, C</creator><creator>Gaven, B</creator><creator>Carron, A</creator><creator>Navarro, J.M</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SS</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20050801</creationdate><title>Growth, Sporulation, δ-Endotoxins Synthesis, and Toxicity During Culture of Bacillus thuringiensis H14</title><author>Sarrafzadeh, M.H ; 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Measurements of optical density and dielectric permittivity for biomass determination suggest a highly promising technique for on-line evaluation of sporulation. The synthesis of 65-, 25- and 130-kDa proteins started at 16, 18, and 23 h, respectively. These proteins were enriched in different ways until the end of culture (48 h). Toxicity in the course of sporulation was significantly different for the larvae of both mosquito species. Maximal activity against Ae. aegypti was obtained at the end of culture, whereas for Cx. pipiens, the sample at 38 h was the most active.</abstract><cop>United States</cop><pmid>16059772</pmid><doi>10.1007/s00284-005-4463-3</doi><tpages>7</tpages></addata></record> |
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| ispartof | Current microbiology, 2005-08, Vol.51 (2), p.75-81 |
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| subjects | Aedes - microbiology Aedes aegypti Animals Bacillus thuringiensis Bacillus thuringiensis - growth & development Bacillus thuringiensis - metabolism Bacillus thuringiensis - physiology bacterial insecticides Bacterial Proteins - metabolism bacterial spores bacterial toxins Bacterial Toxins - metabolism biological control biological control agents biosynthesis crystal proteins Culex Culex pipiens delta-endotoxins Endotoxins - metabolism entomopathogenic bacteria Gram-positive bacteria Hemolysin Proteins larvae mosquito control Mosquito Control - methods Spores, Bacterial sporulation |
| title | Growth, Sporulation, δ-Endotoxins Synthesis, and Toxicity During Culture of Bacillus thuringiensis H14 |
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