Genetic determinants of Sindbis virus strain TR339 affecting midgut infection in the mosquito Aedes aegypti
Mosquito midgut epithelial cells (MEC) play a major role in determining whether an arbovirus can successfully infect and be transmitted by mosquitoes. The Sindbis virus (SINV) strain TR339 efficiently infects Aedes aegypti MEC but the SINV strain TE/5'2J poorly infects MEC. SINV determinants fo...
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| Veröffentlicht in: | Journal of general virology 2007-05, Vol.88 (5), p.1545-1554 |
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| description | Mosquito midgut epithelial cells (MEC) play a major role in determining whether an arbovirus can successfully infect and be transmitted by mosquitoes. The Sindbis virus (SINV) strain TR339 efficiently infects Aedes aegypti MEC but the SINV strain TE/5'2J poorly infects MEC. SINV determinants for MEC infection have been localized to the E2 glycoprotein. The E2 amino acid sequences of TR339 and TE/5'2J differ at two sites, E2-55 and E2-70. We have altered the TE/5'2J virus genome by site-directed mutagenesis to contain two TR339 residues, E2-55 H leads to Q (histidine to glutamine) and E2-70 K leads to E (lysine to glutamic acid). We have characterized the growth patterns of derived viruses in cell culture and determined the midgut infection rate (MIR) in A. aegypti mosquitoes. Our results clearly show that the E2-55 H leads to Q and the E2-70 K leads to E mutations in the TE/5'2J virus increase MIR both independently and in combination. TE/5'2J virus containing both TR339 E2 residues had MIRs similar to the parental TR339 virus. In addition, SINV propagated in a mammalian cell line had a significantly lower A. aegypti midgut 50 % infectious dose than virus propagated in a mosquito cell line. |
| doi_str_mv | 10.1099/vir.0.82577-0 |
| format | Article |
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The Sindbis virus (SINV) strain TR339 efficiently infects Aedes aegypti MEC but the SINV strain TE/5'2J poorly infects MEC. SINV determinants for MEC infection have been localized to the E2 glycoprotein. The E2 amino acid sequences of TR339 and TE/5'2J differ at two sites, E2-55 and E2-70. We have altered the TE/5'2J virus genome by site-directed mutagenesis to contain two TR339 residues, E2-55 H leads to Q (histidine to glutamine) and E2-70 K leads to E (lysine to glutamic acid). We have characterized the growth patterns of derived viruses in cell culture and determined the midgut infection rate (MIR) in A. aegypti mosquitoes. Our results clearly show that the E2-55 H leads to Q and the E2-70 K leads to E mutations in the TE/5'2J virus increase MIR both independently and in combination. TE/5'2J virus containing both TR339 E2 residues had MIRs similar to the parental TR339 virus. In addition, SINV propagated in a mammalian cell line had a significantly lower A. aegypti midgut 50 % infectious dose than virus propagated in a mosquito cell line.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/vir.0.82577-0</identifier><identifier>PMID: 17412985</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Aedes - virology ; Aedes aegypti ; Amino Acid Sequence ; amino acid sequences ; Animal Feed ; Animals ; Arbovirus ; Biological and medical sciences ; Blood ; Digestive System - virology ; envelope glycoproteins ; Epithelial Cells - virology ; epithelium ; Fundamental and applied biological sciences. Psychology ; Genetics ; Genome, Viral ; glycoproteins ; infection ; insect vectors ; Microbiology ; midgut ; Miscellaneous ; Molecular Sequence Data ; Mouth - virology ; mutants ; Sequence Alignment ; Sequence Homology, Amino Acid ; Sindbis virus ; Sindbis Virus - classification ; Sindbis Virus - genetics ; Sindbis Virus - isolation & purification ; site-directed mutagenesis ; strain differences ; viral proteins ; Viral Proteins - chemistry ; Viral Proteins - genetics ; Virology ; virus replication</subject><ispartof>Journal of general virology, 2007-05, Vol.88 (5), p.1545-1554</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-8af7e5e91fb4808407675fe0254648a1cef0395bea463e44d40e9cfa9fa4085f3</citedby><cites>FETCH-LOGICAL-c448t-8af7e5e91fb4808407675fe0254648a1cef0395bea463e44d40e9cfa9fa4085f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3744,3745,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18715338$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17412985$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pierro, D.J</creatorcontrib><creatorcontrib>Powers, E.L</creatorcontrib><creatorcontrib>Olson, K.E</creatorcontrib><title>Genetic determinants of Sindbis virus strain TR339 affecting midgut infection in the mosquito Aedes aegypti</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Mosquito midgut epithelial cells (MEC) play a major role in determining whether an arbovirus can successfully infect and be transmitted by mosquitoes. The Sindbis virus (SINV) strain TR339 efficiently infects Aedes aegypti MEC but the SINV strain TE/5'2J poorly infects MEC. SINV determinants for MEC infection have been localized to the E2 glycoprotein. The E2 amino acid sequences of TR339 and TE/5'2J differ at two sites, E2-55 and E2-70. We have altered the TE/5'2J virus genome by site-directed mutagenesis to contain two TR339 residues, E2-55 H leads to Q (histidine to glutamine) and E2-70 K leads to E (lysine to glutamic acid). We have characterized the growth patterns of derived viruses in cell culture and determined the midgut infection rate (MIR) in A. aegypti mosquitoes. Our results clearly show that the E2-55 H leads to Q and the E2-70 K leads to E mutations in the TE/5'2J virus increase MIR both independently and in combination. TE/5'2J virus containing both TR339 E2 residues had MIRs similar to the parental TR339 virus. In addition, SINV propagated in a mammalian cell line had a significantly lower A. aegypti midgut 50 % infectious dose than virus propagated in a mosquito cell line.</description><subject>Aedes - virology</subject><subject>Aedes aegypti</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Animal Feed</subject><subject>Animals</subject><subject>Arbovirus</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Digestive System - virology</subject><subject>envelope glycoproteins</subject><subject>Epithelial Cells - virology</subject><subject>epithelium</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Genome, Viral</subject><subject>glycoproteins</subject><subject>infection</subject><subject>insect vectors</subject><subject>Microbiology</subject><subject>midgut</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Mouth - virology</subject><subject>mutants</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sindbis virus</subject><subject>Sindbis Virus - classification</subject><subject>Sindbis Virus - genetics</subject><subject>Sindbis Virus - isolation & purification</subject><subject>site-directed mutagenesis</subject><subject>strain differences</subject><subject>viral proteins</subject><subject>Viral Proteins - chemistry</subject><subject>Viral Proteins - genetics</subject><subject>Virology</subject><subject>virus replication</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0M1vFCEYBnBiNHatHr0qF42XWV--FubYNFpNmpjY9kyYmZdZdIfZAlPT_162u0lPEPjl4eUh5D2DNYO2_foQ0hrWhiutG3hBVkxuVMPrzUuyAuC8YYLpM_Im5z8ATEqlX5MzpiXjrVEr8vcKI5bQ0wELpilEF0ums6c3IQ5dyLTmL5nmklyI9Pa3EC113mNfQhzpFIZxKTTEp4M51h0tW6TTnO-XUGZ6gQNm6nB83Jfwlrzybpfx3Wk9J3ffv91e_miuf139vLy4bnopTWmM8xoVtsx30oCRoDdaeQSu5EYax3r0IFrVoZMbgVIOErDtvWu9k2CUF-fk8zF3n-b7BXOxU8g97nYu4rxky4ELBWJTYXOEfZpzTujtPoXJpUfLwB7atfX3FuxTuxaq_3AKXroJh2d9qrOCTyfgcu92PrnYh_zsjGZKCFPdl6PbhnH7LyS0I8Yp1DG6MB8eNcYqy5Q8RH48Uu9m68ZU4-5uODABoI1quRT_AfI0muc</recordid><startdate>20070501</startdate><enddate>20070501</enddate><creator>Pierro, D.J</creator><creator>Powers, E.L</creator><creator>Olson, K.E</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20070501</creationdate><title>Genetic determinants of Sindbis virus strain TR339 affecting midgut infection in the mosquito Aedes aegypti</title><author>Pierro, D.J ; Powers, E.L ; Olson, K.E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-8af7e5e91fb4808407675fe0254648a1cef0395bea463e44d40e9cfa9fa4085f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Aedes - virology</topic><topic>Aedes aegypti</topic><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Animal Feed</topic><topic>Animals</topic><topic>Arbovirus</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Digestive System - virology</topic><topic>envelope glycoproteins</topic><topic>Epithelial Cells - virology</topic><topic>epithelium</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Genome, Viral</topic><topic>glycoproteins</topic><topic>infection</topic><topic>insect vectors</topic><topic>Microbiology</topic><topic>midgut</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Mouth - virology</topic><topic>mutants</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sindbis virus</topic><topic>Sindbis Virus - classification</topic><topic>Sindbis Virus - genetics</topic><topic>Sindbis Virus - isolation & purification</topic><topic>site-directed mutagenesis</topic><topic>strain differences</topic><topic>viral proteins</topic><topic>Viral Proteins - chemistry</topic><topic>Viral Proteins - genetics</topic><topic>Virology</topic><topic>virus replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pierro, D.J</creatorcontrib><creatorcontrib>Powers, E.L</creatorcontrib><creatorcontrib>Olson, K.E</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pierro, D.J</au><au>Powers, E.L</au><au>Olson, K.E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic determinants of Sindbis virus strain TR339 affecting midgut infection in the mosquito Aedes aegypti</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>2007-05-01</date><risdate>2007</risdate><volume>88</volume><issue>5</issue><spage>1545</spage><epage>1554</epage><pages>1545-1554</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>Mosquito midgut epithelial cells (MEC) play a major role in determining whether an arbovirus can successfully infect and be transmitted by mosquitoes. The Sindbis virus (SINV) strain TR339 efficiently infects Aedes aegypti MEC but the SINV strain TE/5'2J poorly infects MEC. SINV determinants for MEC infection have been localized to the E2 glycoprotein. The E2 amino acid sequences of TR339 and TE/5'2J differ at two sites, E2-55 and E2-70. We have altered the TE/5'2J virus genome by site-directed mutagenesis to contain two TR339 residues, E2-55 H leads to Q (histidine to glutamine) and E2-70 K leads to E (lysine to glutamic acid). We have characterized the growth patterns of derived viruses in cell culture and determined the midgut infection rate (MIR) in A. aegypti mosquitoes. Our results clearly show that the E2-55 H leads to Q and the E2-70 K leads to E mutations in the TE/5'2J virus increase MIR both independently and in combination. TE/5'2J virus containing both TR339 E2 residues had MIRs similar to the parental TR339 virus. In addition, SINV propagated in a mammalian cell line had a significantly lower A. aegypti midgut 50 % infectious dose than virus propagated in a mosquito cell line.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>17412985</pmid><doi>10.1099/vir.0.82577-0</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aedes - virology Aedes aegypti Amino Acid Sequence amino acid sequences Animal Feed Animals Arbovirus Biological and medical sciences Blood Digestive System - virology envelope glycoproteins Epithelial Cells - virology epithelium Fundamental and applied biological sciences. Psychology Genetics Genome, Viral glycoproteins infection insect vectors Microbiology midgut Miscellaneous Molecular Sequence Data Mouth - virology mutants Sequence Alignment Sequence Homology, Amino Acid Sindbis virus Sindbis Virus - classification Sindbis Virus - genetics Sindbis Virus - isolation & purification site-directed mutagenesis strain differences viral proteins Viral Proteins - chemistry Viral Proteins - genetics Virology virus replication |
| title | Genetic determinants of Sindbis virus strain TR339 affecting midgut infection in the mosquito Aedes aegypti |
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