Purification of human erythropoietin by affinity chromatography using cyclic peptide ligands

Prior work described the identification and characterization of erythropoietin-binding cyclic peptides SLFFLH, VVFFVH, FSLLHH and FSLLSH (all of the form cyclo[(Nα-Ac)Dap(A)-X1-X6-AE], wherein X1-X6 is the listed sequences). In this work, the peptide ligands were synthesized on Toyopearl chromatogra...

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Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2018-05, Vol.1085, p.1-12
Hauptverfasser:	Kish, William S., Roach, Matthew K., Sachi, Hiroyuki, Naik, Amith D., Menegatti, Stefano, Carbonell, Ruben G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity chromatography Animals Chinese hamster ovary CHO Cells Chromatography, Affinity - methods Cricetinae Cricetulus Cyclic peptide ligand Erythropoietin - analysis Erythropoietin - chemistry Erythropoietin - isolation & purification Erythropoietin - metabolism Erythropoietin purification Humans Hydrogen-Ion Concentration Peptides, Cyclic - chemistry Peptides, Cyclic - metabolism Pichia Pichia pastoris Proteins
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