Establishment and proteomic characterization of a novel synovial sarcoma cell line, NCC-SS2-C1
Synovial sarcoma is an aggressive mesenchymal tumor, characterized by the presence of unique transfusion gene, SS18–SSX. Cell lines enable researchers to investigate the molecular backgrounds of disease and the significance of SS18–SSX in relevant cellular contexts. We report the establishment and p...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Animal 2018-05, Vol.54 (5), p.392-399 |
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| creator | Oyama, Rieko Kito, Fusako Sakumoto, Marimu Shiozawa, Kumiko Toki, Shunichi Endo, Makoto Yoshida, Akihiko Kawai, Akira Kondo, Tadashi |
| description | Synovial sarcoma is an aggressive mesenchymal tumor, characterized by the presence of unique transfusion gene, SS18–SSX. Cell lines enable researchers to investigate the molecular backgrounds of disease and the significance of SS18–SSX in relevant cellular contexts. We report the establishment and proteomic characterization of a novel synovial sarcoma cell line. Primary tissue culture was performed using tumor tissue of synovial sarcoma. The established cell line was authenticated by assessing its DNA microsatellite short tandem repeat analysis and characterized by in vitro assay. Proteomic study was achieved by mass spectrometry, and the results were analyzed by treemap. The cell line NCC-SS2-C1 was established from a primary tumor tissue of a synovial sarcoma patient. The cell line has grown well for 11 mo and has been subcultured more than 15 times. The established cells were authenticated by assessing their short tandem repeat pattern comparing with that of original tumor tissue. The cells showed polygonal in shape and formed spheroid when seeded on the low-attachment dish. Proteomic analysis revealed the molecular pathways which are unique to the original tumor tissue or the established cell line. In conclusion, a novel synovial sarcoma cell line NCC-SS2-C1 was successfully established from the primary tumor tissue. The cell line has characteristic transfusion SS18–SSX and poses aggressive in vitro growth and capability of spheroid formation. Thus, NCC-SS2-C1 cell line will be a useful tool for investigation of the mechanisms of disease and the biological role of fusion gene. |
| doi_str_mv | 10.1007/s11626-018-0237-7 |
| format | Article |
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Cell lines enable researchers to investigate the molecular backgrounds of disease and the significance of SS18–SSX in relevant cellular contexts. We report the establishment and proteomic characterization of a novel synovial sarcoma cell line. Primary tissue culture was performed using tumor tissue of synovial sarcoma. The established cell line was authenticated by assessing its DNA microsatellite short tandem repeat analysis and characterized by in vitro assay. Proteomic study was achieved by mass spectrometry, and the results were analyzed by treemap. The cell line NCC-SS2-C1 was established from a primary tumor tissue of a synovial sarcoma patient. The cell line has grown well for 11 mo and has been subcultured more than 15 times. The established cells were authenticated by assessing their short tandem repeat pattern comparing with that of original tumor tissue. The cells showed polygonal in shape and formed spheroid when seeded on the low-attachment dish. Proteomic analysis revealed the molecular pathways which are unique to the original tumor tissue or the established cell line. In conclusion, a novel synovial sarcoma cell line NCC-SS2-C1 was successfully established from the primary tumor tissue. The cell line has characteristic transfusion SS18–SSX and poses aggressive in vitro growth and capability of spheroid formation. Thus, NCC-SS2-C1 cell line will be a useful tool for investigation of the mechanisms of disease and the biological role of fusion gene.</description><identifier>ISSN: 1071-2690</identifier><identifier>EISSN: 1543-706X</identifier><identifier>DOI: 10.1007/s11626-018-0237-7</identifier><identifier>PMID: 29626278</identifier><language>eng</language><publisher>New York: Springer Science & Business Media LLC</publisher><subject>Animal Genetics and Genomics ; Biomedical and Life Sciences ; Cancer therapies ; Cell Biology ; Cell Culture ; Chemotherapy ; Deoxyribonucleic acid ; Developmental Biology ; DNA ; ESTABLISHMENT OF CELL LINES ; Fusion protein ; Gene expression ; Life Sciences ; Mass spectrometry ; Mass spectroscopy ; Medical prognosis ; Mesenchyme ; Patients ; Sarcoma ; Stem Cells ; Studies ; Synovial sarcoma ; Tissue culture ; Tissues ; Transfusion ; Tumors</subject><ispartof>In vitro cellular & developmental biology. Animal, 2018-05, Vol.54 (5), p.392-399</ispartof><rights>2018 Society for In Vitro Biology</rights><rights>The Society for In Vitro Biology 2018</rights><rights>Copyright Society for In Vitro Biology May 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-3678887e9427f9f6b136b98685171a28c0d6ecaf278cba9ee298aff79a37e0823</citedby><cites>FETCH-LOGICAL-c460t-3678887e9427f9f6b136b98685171a28c0d6ecaf278cba9ee298aff79a37e0823</cites><orcidid>0000-0001-6405-7792</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26578099$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26578099$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,41488,42557,51319,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29626278$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oyama, Rieko</creatorcontrib><creatorcontrib>Kito, Fusako</creatorcontrib><creatorcontrib>Sakumoto, Marimu</creatorcontrib><creatorcontrib>Shiozawa, Kumiko</creatorcontrib><creatorcontrib>Toki, Shunichi</creatorcontrib><creatorcontrib>Endo, Makoto</creatorcontrib><creatorcontrib>Yoshida, Akihiko</creatorcontrib><creatorcontrib>Kawai, Akira</creatorcontrib><creatorcontrib>Kondo, Tadashi</creatorcontrib><title>Establishment and proteomic characterization of a novel synovial sarcoma cell line, NCC-SS2-C1</title><title>In vitro cellular & developmental biology. Animal</title><addtitle>In Vitro Cell.Dev.Biol.-Animal</addtitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><description>Synovial sarcoma is an aggressive mesenchymal tumor, characterized by the presence of unique transfusion gene, SS18–SSX. Cell lines enable researchers to investigate the molecular backgrounds of disease and the significance of SS18–SSX in relevant cellular contexts. We report the establishment and proteomic characterization of a novel synovial sarcoma cell line. Primary tissue culture was performed using tumor tissue of synovial sarcoma. The established cell line was authenticated by assessing its DNA microsatellite short tandem repeat analysis and characterized by in vitro assay. Proteomic study was achieved by mass spectrometry, and the results were analyzed by treemap. The cell line NCC-SS2-C1 was established from a primary tumor tissue of a synovial sarcoma patient. The cell line has grown well for 11 mo and has been subcultured more than 15 times. The established cells were authenticated by assessing their short tandem repeat pattern comparing with that of original tumor tissue. The cells showed polygonal in shape and formed spheroid when seeded on the low-attachment dish. Proteomic analysis revealed the molecular pathways which are unique to the original tumor tissue or the established cell line. In conclusion, a novel synovial sarcoma cell line NCC-SS2-C1 was successfully established from the primary tumor tissue. The cell line has characteristic transfusion SS18–SSX and poses aggressive in vitro growth and capability of spheroid formation. Thus, NCC-SS2-C1 cell line will be a useful tool for investigation of the mechanisms of disease and the biological role of fusion gene.</description><subject>Animal Genetics and Genomics</subject><subject>Biomedical and Life Sciences</subject><subject>Cancer therapies</subject><subject>Cell Biology</subject><subject>Cell Culture</subject><subject>Chemotherapy</subject><subject>Deoxyribonucleic acid</subject><subject>Developmental Biology</subject><subject>DNA</subject><subject>ESTABLISHMENT OF CELL LINES</subject><subject>Fusion protein</subject><subject>Gene expression</subject><subject>Life Sciences</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical prognosis</subject><subject>Mesenchyme</subject><subject>Patients</subject><subject>Sarcoma</subject><subject>Stem Cells</subject><subject>Studies</subject><subject>Synovial sarcoma</subject><subject>Tissue culture</subject><subject>Tissues</subject><subject>Transfusion</subject><subject>Tumors</subject><issn>1071-2690</issn><issn>1543-706X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kEtLHEEUhQtRfEz8AS4MBdlkkTL1mKnHUhpjAhIXGsjK4nbNbe2hu8tU9QTMr7eGHiO4sDb3Qp1z7uEj5ETwM8G5-ZqF0FIzLizjUhlmdsihWMwVM1z_3i07N4JJ7fgBOcp5xctzQu-TA-mKTxp7SO4u8gh11-aHHoeRwrCkjymOGPs20PAACcKIqf0HYxsHGhsKdIh_saP5qcwWygIpxB5owK6jXTvgF_qzqtjNjWSV-ED2GugyHm_njPz6dnFbfWdX15c_qvMrFuaaj0xpY6016ObSNK7RtVC6dlbbhTACpA18qTFAUyqHGhyidBaaxjhQBrmVakY-T7ml_J815tH3bd40ggHjOnvJpXTGzoUp0k9vpKu4TkNp56VQ3BQ2ShWVmFQhxZwTNv4xtT2kJy-438D3E3xf4PsNfL9J_rhNXtc9Lv87XmgXgZwEuXwN95heT7-XejqZVnmM6TVUL4zlzqlnvGWXOQ</recordid><startdate>20180501</startdate><enddate>20180501</enddate><creator>Oyama, Rieko</creator><creator>Kito, Fusako</creator><creator>Sakumoto, Marimu</creator><creator>Shiozawa, Kumiko</creator><creator>Toki, Shunichi</creator><creator>Endo, Makoto</creator><creator>Yoshida, Akihiko</creator><creator>Kawai, Akira</creator><creator>Kondo, Tadashi</creator><general>Springer Science & Business Media LLC</general><general>Springer US</general><general>Society for In Vitro Biology</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6405-7792</orcidid></search><sort><creationdate>20180501</creationdate><title>Establishment and proteomic characterization of a novel synovial sarcoma cell line, NCC-SS2-C1</title><author>Oyama, Rieko ; 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Animal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oyama, Rieko</au><au>Kito, Fusako</au><au>Sakumoto, Marimu</au><au>Shiozawa, Kumiko</au><au>Toki, Shunichi</au><au>Endo, Makoto</au><au>Yoshida, Akihiko</au><au>Kawai, Akira</au><au>Kondo, Tadashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment and proteomic characterization of a novel synovial sarcoma cell line, NCC-SS2-C1</atitle><jtitle>In vitro cellular & developmental biology. Animal</jtitle><stitle>In Vitro Cell.Dev.Biol.-Animal</stitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><date>2018-05-01</date><risdate>2018</risdate><volume>54</volume><issue>5</issue><spage>392</spage><epage>399</epage><pages>392-399</pages><issn>1071-2690</issn><eissn>1543-706X</eissn><abstract>Synovial sarcoma is an aggressive mesenchymal tumor, characterized by the presence of unique transfusion gene, SS18–SSX. Cell lines enable researchers to investigate the molecular backgrounds of disease and the significance of SS18–SSX in relevant cellular contexts. We report the establishment and proteomic characterization of a novel synovial sarcoma cell line. Primary tissue culture was performed using tumor tissue of synovial sarcoma. The established cell line was authenticated by assessing its DNA microsatellite short tandem repeat analysis and characterized by in vitro assay. Proteomic study was achieved by mass spectrometry, and the results were analyzed by treemap. The cell line NCC-SS2-C1 was established from a primary tumor tissue of a synovial sarcoma patient. The cell line has grown well for 11 mo and has been subcultured more than 15 times. The established cells were authenticated by assessing their short tandem repeat pattern comparing with that of original tumor tissue. The cells showed polygonal in shape and formed spheroid when seeded on the low-attachment dish. Proteomic analysis revealed the molecular pathways which are unique to the original tumor tissue or the established cell line. In conclusion, a novel synovial sarcoma cell line NCC-SS2-C1 was successfully established from the primary tumor tissue. The cell line has characteristic transfusion SS18–SSX and poses aggressive in vitro growth and capability of spheroid formation. Thus, NCC-SS2-C1 cell line will be a useful tool for investigation of the mechanisms of disease and the biological role of fusion gene.</abstract><cop>New York</cop><pub>Springer Science & Business Media LLC</pub><pmid>29626278</pmid><doi>10.1007/s11626-018-0237-7</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-6405-7792</orcidid></addata></record> |
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| subjects | Animal Genetics and Genomics Biomedical and Life Sciences Cancer therapies Cell Biology Cell Culture Chemotherapy Deoxyribonucleic acid Developmental Biology DNA ESTABLISHMENT OF CELL LINES Fusion protein Gene expression Life Sciences Mass spectrometry Mass spectroscopy Medical prognosis Mesenchyme Patients Sarcoma Stem Cells Studies Synovial sarcoma Tissue culture Tissues Transfusion Tumors |
| title | Establishment and proteomic characterization of a novel synovial sarcoma cell line, NCC-SS2-C1 |
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