Acute and chronic effects of organophosphate pesticides (Basudin) to amphibian tadpoles (Ptychadena bibroni)

Toxicity of basudin (an organophosphate pesticide) on the larval stages of the dominant amphibian; Ptychadena bibroni of the Niger Delta ecological zone of Nigeria was assessed using acute and chronic toxicity in the laboratory. Mortality and body glycogen levels were used as ecological endpoints. The American society of testing and material (ASTM) recommended semi-static renewal bioassay was employed and LC sub(50) was measured at 96 h. The amphibian larval stages were exposed to basudin concentrations of 0.1, 1.0, 10.0 and 25 ug/l. Derived 96 h LC sub(50) values decreased with increased exposure duration. Estimated 96 h LC sub(50) was 0.860 ug/l. Substantial mortality and incidence of abnormal avoidance response occurred more at higher concentrations. Body glycogen levels in all test concentrations were significantly different between the test and the control experiments at P