Clonal conditional mutagenesis in malaria parasites

We describe here an efficient method for conditional gene inactivation in malaria parasites that uses the Flp/FRT site-specific recombination system of yeast. The method, developed in Plasmodium berghei, consists of inserting FRT sites in the chromosomal locus of interest in a parasite clone express...

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Veröffentlicht in:	Cell host & microbe 2009-04, Vol.5 (4), p.386-396
Hauptverfasser:	Combe, Audrey, Giovannini, Donatella, Carvalho, Teresa Gil, Spath, Stephan, Boisson, Bertrand, Loussert, Céline, Thiberge, Sabine, Lacroix, Céline, Gueirard, Pascale, Ménard, Robert
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals DNA Nucleotidyltransferases - genetics DNA Nucleotidyltransferases - metabolism Gene Deletion Molecular Biology - methods Mutagenesis Plasmodium berghei Plasmodium berghei - genetics Recombination, Genetic
Online-Zugang:	Volltext
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