Monoclonal Antibody L1Mab-13 Detected Human PD-L1 in Lung Cancers
Programmed cell death ligand-1 (PD-L1) is a type I transmembrane glycoprotein expressed on antigen-presenting cells. It is also expressed in several tumor cells such as melanoma and lung cancer cells. A strong correlation has been reported between human PD-L1 (hPD-L1) expression in tumor cells and n...
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| Veröffentlicht in: | Monoclonal antibodies in immunodiagnosis and immunotherapy 2018-04, Vol.37 (2), p.11-115 |
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| container_title | Monoclonal antibodies in immunodiagnosis and immunotherapy |
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| creator | Yamada, Shinji Itai, Shunsuke Nakamura, Takuro Yanaka, Miyuki Chang, Yao-Wen Suzuki, Hiroyoshi Kaneko, Mika K. Kato, Yukinari |
| description | Programmed cell death ligand-1 (PD-L1) is a type I transmembrane glycoprotein expressed on antigen-presenting cells. It is also expressed in several tumor cells such as melanoma and lung cancer cells. A strong correlation has been reported between human PD-L1 (hPD-L1) expression in tumor cells and negative prognosis in cancer patients. Here, a novel anti-hPD-L1 monoclonal antibody (mAb) L
1
Mab-13 (IgG
1
, kappa) was produced using a cell-based immunization and screening (CBIS) method. We investigated hPD-L1 expression in lung cancer using flow cytometry, Western blot, and immunohistochemical analyses. L
1
Mab-13 specifically reacted hPD-L1 of hPD-L1-overexpressed Chinese hamster ovary (CHO)-K1 cells and endogenous hPD-L1 of KMST-6 (human fibroblast) in flow cytometry and Western blot. Furthermore, L
1
Mab-13 reacted with lung cancer cell lines (EBC-1, Lu65, and Lu99) in flow cytometry and stained lung cancer tissues in a membrane-staining pattern in immunohistochemical analysis. These results indicate that a novel anti-hPD-L1 mAb, L
1
Mab-13, is very useful for detecting hPD-L1 of lung cancers in flow cytometry, Western blot, and immunohistochemical analyses. |
| doi_str_mv | 10.1089/mab.2018.0004 |
| format | Article |
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1
Mab-13 (IgG
1
, kappa) was produced using a cell-based immunization and screening (CBIS) method. We investigated hPD-L1 expression in lung cancer using flow cytometry, Western blot, and immunohistochemical analyses. L
1
Mab-13 specifically reacted hPD-L1 of hPD-L1-overexpressed Chinese hamster ovary (CHO)-K1 cells and endogenous hPD-L1 of KMST-6 (human fibroblast) in flow cytometry and Western blot. Furthermore, L
1
Mab-13 reacted with lung cancer cell lines (EBC-1, Lu65, and Lu99) in flow cytometry and stained lung cancer tissues in a membrane-staining pattern in immunohistochemical analysis. These results indicate that a novel anti-hPD-L1 mAb, L
1
Mab-13, is very useful for detecting hPD-L1 of lung cancers in flow cytometry, Western blot, and immunohistochemical analyses.</description><identifier>EISSN: 2167-9436</identifier><identifier>DOI: 10.1089/mab.2018.0004</identifier><language>eng</language><publisher>New Rochelle: Mary Ann Liebert, Inc</publisher><subject>Antigen-presenting cells ; Apoptosis ; Cancer ; Cell death ; Cytometry ; Flow cytometry ; Glycoproteins ; Immunization ; Immunoglobulin G ; Lung cancer ; Melanoma ; Monoclonal antibodies ; PD-L1 protein ; Short Communications ; Tumor cell lines ; Tumor cells ; Tumors</subject><ispartof>Monoclonal antibodies in immunodiagnosis and immunotherapy, 2018-04, Vol.37 (2), p.11-115</ispartof><rights>2018, Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2018, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Yamada, Shinji</creatorcontrib><creatorcontrib>Itai, Shunsuke</creatorcontrib><creatorcontrib>Nakamura, Takuro</creatorcontrib><creatorcontrib>Yanaka, Miyuki</creatorcontrib><creatorcontrib>Chang, Yao-Wen</creatorcontrib><creatorcontrib>Suzuki, Hiroyoshi</creatorcontrib><creatorcontrib>Kaneko, Mika K.</creatorcontrib><creatorcontrib>Kato, Yukinari</creatorcontrib><title>Monoclonal Antibody L1Mab-13 Detected Human PD-L1 in Lung Cancers</title><title>Monoclonal antibodies in immunodiagnosis and immunotherapy</title><description>Programmed cell death ligand-1 (PD-L1) is a type I transmembrane glycoprotein expressed on antigen-presenting cells. It is also expressed in several tumor cells such as melanoma and lung cancer cells. A strong correlation has been reported between human PD-L1 (hPD-L1) expression in tumor cells and negative prognosis in cancer patients. Here, a novel anti-hPD-L1 monoclonal antibody (mAb) L
1
Mab-13 (IgG
1
, kappa) was produced using a cell-based immunization and screening (CBIS) method. We investigated hPD-L1 expression in lung cancer using flow cytometry, Western blot, and immunohistochemical analyses. L
1
Mab-13 specifically reacted hPD-L1 of hPD-L1-overexpressed Chinese hamster ovary (CHO)-K1 cells and endogenous hPD-L1 of KMST-6 (human fibroblast) in flow cytometry and Western blot. Furthermore, L
1
Mab-13 reacted with lung cancer cell lines (EBC-1, Lu65, and Lu99) in flow cytometry and stained lung cancer tissues in a membrane-staining pattern in immunohistochemical analysis. These results indicate that a novel anti-hPD-L1 mAb, L
1
Mab-13, is very useful for detecting hPD-L1 of lung cancers in flow cytometry, Western blot, and immunohistochemical analyses.</description><subject>Antigen-presenting cells</subject><subject>Apoptosis</subject><subject>Cancer</subject><subject>Cell death</subject><subject>Cytometry</subject><subject>Flow cytometry</subject><subject>Glycoproteins</subject><subject>Immunization</subject><subject>Immunoglobulin G</subject><subject>Lung cancer</subject><subject>Melanoma</subject><subject>Monoclonal antibodies</subject><subject>PD-L1 protein</subject><subject>Short Communications</subject><subject>Tumor cell lines</subject><subject>Tumor cells</subject><subject>Tumors</subject><issn>2167-9436</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpdkDFrwzAQhUWh0JBm7C7o0sXpneTI0hiStik4tEM7C8mRioMtp5Y95N9XJpk6HTw-3vE-Qh4QlghSPbfGLhmgXAJAfkNmDEWRqZyLO7KI8ZhSKJAhX83Iet-Frmq6YBq6DkNtu8OZlrg3NkNOt25w1eAOdDe2JtDPbVYirQMtx_BDNyZUro_35NabJrrF9c7J9-vL12aXlR9v75t1mbVYrIZMWMUQPHeghLfSV15IaTwWgMoo4AJAWAG2UrLAXFprCstybgqPYCRnfE6eLr2nvvsdXRx0W8fKNY0JrhujZpAWMZGnrjl5_Iceu7FPEyeKsxWq9CRR_EK1pj-bEJraWdcP-tTXU6IR9GRTJ5t6sqknm_wP4G1lzQ</recordid><startdate>20180401</startdate><enddate>20180401</enddate><creator>Yamada, Shinji</creator><creator>Itai, Shunsuke</creator><creator>Nakamura, Takuro</creator><creator>Yanaka, Miyuki</creator><creator>Chang, Yao-Wen</creator><creator>Suzuki, Hiroyoshi</creator><creator>Kaneko, Mika K.</creator><creator>Kato, Yukinari</creator><general>Mary Ann Liebert, Inc</general><scope>7QL</scope><scope>7QO</scope><scope>7T5</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20180401</creationdate><title>Monoclonal Antibody L1Mab-13 Detected Human PD-L1 in Lung Cancers</title><author>Yamada, Shinji ; Itai, Shunsuke ; Nakamura, Takuro ; Yanaka, Miyuki ; Chang, Yao-Wen ; Suzuki, Hiroyoshi ; Kaneko, Mika K. ; Kato, Yukinari</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-m175t-6b9210f3e096fb8fcf688af17019a9036006b60bc987148bba7b243a7f10a8323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Antigen-presenting cells</topic><topic>Apoptosis</topic><topic>Cancer</topic><topic>Cell death</topic><topic>Cytometry</topic><topic>Flow cytometry</topic><topic>Glycoproteins</topic><topic>Immunization</topic><topic>Immunoglobulin G</topic><topic>Lung cancer</topic><topic>Melanoma</topic><topic>Monoclonal antibodies</topic><topic>PD-L1 protein</topic><topic>Short Communications</topic><topic>Tumor cell lines</topic><topic>Tumor cells</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamada, Shinji</creatorcontrib><creatorcontrib>Itai, Shunsuke</creatorcontrib><creatorcontrib>Nakamura, Takuro</creatorcontrib><creatorcontrib>Yanaka, Miyuki</creatorcontrib><creatorcontrib>Chang, Yao-Wen</creatorcontrib><creatorcontrib>Suzuki, Hiroyoshi</creatorcontrib><creatorcontrib>Kaneko, Mika K.</creatorcontrib><creatorcontrib>Kato, Yukinari</creatorcontrib><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Monoclonal antibodies in immunodiagnosis and immunotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamada, Shinji</au><au>Itai, Shunsuke</au><au>Nakamura, Takuro</au><au>Yanaka, Miyuki</au><au>Chang, Yao-Wen</au><au>Suzuki, Hiroyoshi</au><au>Kaneko, Mika K.</au><au>Kato, Yukinari</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monoclonal Antibody L1Mab-13 Detected Human PD-L1 in Lung Cancers</atitle><jtitle>Monoclonal antibodies in immunodiagnosis and immunotherapy</jtitle><date>2018-04-01</date><risdate>2018</risdate><volume>37</volume><issue>2</issue><spage>11</spage><epage>115</epage><pages>11-115</pages><eissn>2167-9436</eissn><abstract>Programmed cell death ligand-1 (PD-L1) is a type I transmembrane glycoprotein expressed on antigen-presenting cells. It is also expressed in several tumor cells such as melanoma and lung cancer cells. A strong correlation has been reported between human PD-L1 (hPD-L1) expression in tumor cells and negative prognosis in cancer patients. Here, a novel anti-hPD-L1 monoclonal antibody (mAb) L
1
Mab-13 (IgG
1
, kappa) was produced using a cell-based immunization and screening (CBIS) method. We investigated hPD-L1 expression in lung cancer using flow cytometry, Western blot, and immunohistochemical analyses. L
1
Mab-13 specifically reacted hPD-L1 of hPD-L1-overexpressed Chinese hamster ovary (CHO)-K1 cells and endogenous hPD-L1 of KMST-6 (human fibroblast) in flow cytometry and Western blot. Furthermore, L
1
Mab-13 reacted with lung cancer cell lines (EBC-1, Lu65, and Lu99) in flow cytometry and stained lung cancer tissues in a membrane-staining pattern in immunohistochemical analysis. These results indicate that a novel anti-hPD-L1 mAb, L
1
Mab-13, is very useful for detecting hPD-L1 of lung cancers in flow cytometry, Western blot, and immunohistochemical analyses.</abstract><cop>New Rochelle</cop><pub>Mary Ann Liebert, Inc</pub><doi>10.1089/mab.2018.0004</doi><tpages>105</tpages></addata></record> |
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| source | Alma/SFX Local Collection |
| subjects | Antigen-presenting cells Apoptosis Cancer Cell death Cytometry Flow cytometry Glycoproteins Immunization Immunoglobulin G Lung cancer Melanoma Monoclonal antibodies PD-L1 protein Short Communications Tumor cell lines Tumor cells Tumors |
| title | Monoclonal Antibody L1Mab-13 Detected Human PD-L1 in Lung Cancers |
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