The Protective Immune Response against Infectious Bronchitis Virus Induced by Multi-Epitope Based Peptide Vaccines
Peptide vaccine was found to be an effective and powerful approach to a variety of pathogens. To explore multi-epitope based peptide vaccines against infectious bronchitis virus (IBV), the immunogenic peptides were fused to the 3′ terminal of glutathione S transferase gene (GST) and expressed in Esc...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2009-07, Vol.73 (7), p.1500-1504 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | YANG, Tai WANG, Hong-Ning WANG, Xue TANG, Jun-Ni LU, Dan ZHANG, Yun-Fei GUO, Zi-Cheng LI, Yu-Ling GAO, Rong KANG, Run-Min |
| description | Peptide vaccine was found to be an effective and powerful approach to a variety of pathogens. To explore multi-epitope based peptide vaccines against infectious bronchitis virus (IBV), the immunogenic peptides were fused to the 3′ terminal of glutathione S transferase gene (GST) and expressed in Escherichia coli. ELISA and Western blot analysis showed that the purified fusion proteins had excellent immune activity with chicken anti-IBV serum. During the vaccination course, the candidate peptide vaccines induced strong humoral and cellular response, and provided up to 80.0% immune protection, while all non-immunized chickens in the negative control group manifested obvious typical symptoms and died after virus challenge. Our finding provides a new way to develop multi-epitope based peptide vaccine against IBV. |
| doi_str_mv | 10.1271/bbb.80864 |
| format | Article |
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To explore multi-epitope based peptide vaccines against infectious bronchitis virus (IBV), the immunogenic peptides were fused to the 3′ terminal of glutathione S transferase gene (GST) and expressed in Escherichia coli. ELISA and Western blot analysis showed that the purified fusion proteins had excellent immune activity with chicken anti-IBV serum. During the vaccination course, the candidate peptide vaccines induced strong humoral and cellular response, and provided up to 80.0% immune protection, while all non-immunized chickens in the negative control group manifested obvious typical symptoms and died after virus challenge. Our finding provides a new way to develop multi-epitope based peptide vaccine against IBV.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.80864</identifier><identifier>PMID: 19584555</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Animals ; Antibodies - analysis ; Antibodies - immunology ; Antigens, Viral - chemistry ; Antigens, Viral - immunology ; Biological and medical sciences ; Blotting, Western ; Cell Proliferation ; Chickens - immunology ; Chickens - virology ; Coronavirus Infections - immunology ; Coronavirus Infections - prevention & control ; Coronavirus Infections - virology ; Enzyme-Linked Immunosorbent Assay ; Epitopes - immunology ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Immunity, Humoral - immunology ; Infectious bronchitis virus ; Infectious bronchitis virus - immunology ; Infectious bronchitis virus - physiology ; Leukocytes, Mononuclear - cytology ; Leukocytes, Mononuclear - immunology ; multi-epitope ; peptide vaccine ; Vaccines, Subunit - immunology</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2009-07, Vol.73 (7), p.1500-1504</ispartof><rights>2009 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2009</rights><rights>2009 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c600t-7ffd4867217832aae59b47528777ea3ee31e2d7ab9523d67348054a86a0867793</citedby><cites>FETCH-LOGICAL-c600t-7ffd4867217832aae59b47528777ea3ee31e2d7ab9523d67348054a86a0867793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21884971$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19584555$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YANG, Tai</creatorcontrib><creatorcontrib>WANG, Hong-Ning</creatorcontrib><creatorcontrib>WANG, Xue</creatorcontrib><creatorcontrib>TANG, Jun-Ni</creatorcontrib><creatorcontrib>LU, Dan</creatorcontrib><creatorcontrib>ZHANG, Yun-Fei</creatorcontrib><creatorcontrib>GUO, Zi-Cheng</creatorcontrib><creatorcontrib>LI, Yu-Ling</creatorcontrib><creatorcontrib>GAO, Rong</creatorcontrib><creatorcontrib>KANG, Run-Min</creatorcontrib><title>The Protective Immune Response against Infectious Bronchitis Virus Induced by Multi-Epitope Based Peptide Vaccines</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Peptide vaccine was found to be an effective and powerful approach to a variety of pathogens. To explore multi-epitope based peptide vaccines against infectious bronchitis virus (IBV), the immunogenic peptides were fused to the 3′ terminal of glutathione S transferase gene (GST) and expressed in Escherichia coli. ELISA and Western blot analysis showed that the purified fusion proteins had excellent immune activity with chicken anti-IBV serum. During the vaccination course, the candidate peptide vaccines induced strong humoral and cellular response, and provided up to 80.0% immune protection, while all non-immunized chickens in the negative control group manifested obvious typical symptoms and died after virus challenge. Our finding provides a new way to develop multi-epitope based peptide vaccine against IBV.</description><subject>Animals</subject><subject>Antibodies - analysis</subject><subject>Antibodies - immunology</subject><subject>Antigens, Viral - chemistry</subject><subject>Antigens, Viral - immunology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cell Proliferation</subject><subject>Chickens - immunology</subject><subject>Chickens - virology</subject><subject>Coronavirus Infections - immunology</subject><subject>Coronavirus Infections - prevention & control</subject><subject>Coronavirus Infections - virology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitopes - immunology</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunity, Humoral - immunology</subject><subject>Infectious bronchitis virus</subject><subject>Infectious bronchitis virus - immunology</subject><subject>Infectious bronchitis virus - physiology</subject><subject>Leukocytes, Mononuclear - cytology</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>multi-epitope</subject><subject>peptide vaccine</subject><subject>Vaccines, Subunit - immunology</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90V9r1TAYBvAgDnc2vfALSEBUvOjM3ya9dGPqgYlD5m5Lmr51GW1Sk1Q5337ZztHBBl4FHn55w5sHoZeUHFGm6Ieu64400bV4glaUC1XVjVBP0Yo0tK60kHQfHaR0TUgJJH2G9mkjSyzlCsWLK8DnMWSw2f0GvJ6mxQP-DmkOPgE2P43zKeO1H25FWBI-jsHbK5ddwpculmDt-8VCj7sN_rqM2VWns8thBnxsUonPYc6uB3xprHUe0nO0N5gxwYvdeYh-fDq9OPlSnX37vD75eFbZmpBcqWHoha4Vo0pzZgzIphNKMq2UAsMBOAXWK9M1kvG-VlxoIoXRtSk_oVTDD9G77dw5hl8LpNxOLlkYR-Oh7NEqzqUiDamLfPtfyQijhGpe4OsH8Dos0ZctWipEowXhd-Peb5WNIaUIQztHN5m4aSlpbwtrS2HtXWHFvtpNXLoJ-nu5a6iANztgkjXjEI23Lv1zjGotGkWLE1vn_BDiZP6EOPZtNpsxxL-X-OP3bwByR65e</recordid><startdate>20090701</startdate><enddate>20090701</enddate><creator>YANG, Tai</creator><creator>WANG, Hong-Ning</creator><creator>WANG, Xue</creator><creator>TANG, Jun-Ni</creator><creator>LU, Dan</creator><creator>ZHANG, Yun-Fei</creator><creator>GUO, Zi-Cheng</creator><creator>LI, Yu-Ling</creator><creator>GAO, Rong</creator><creator>KANG, Run-Min</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20090701</creationdate><title>The Protective Immune Response against Infectious Bronchitis Virus Induced by Multi-Epitope Based Peptide Vaccines</title><author>YANG, Tai ; WANG, Hong-Ning ; WANG, Xue ; TANG, Jun-Ni ; LU, Dan ; ZHANG, Yun-Fei ; GUO, Zi-Cheng ; LI, Yu-Ling ; GAO, Rong ; KANG, Run-Min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c600t-7ffd4867217832aae59b47528777ea3ee31e2d7ab9523d67348054a86a0867793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Antibodies - analysis</topic><topic>Antibodies - immunology</topic><topic>Antigens, Viral - chemistry</topic><topic>Antigens, Viral - immunology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cell Proliferation</topic><topic>Chickens - immunology</topic><topic>Chickens - virology</topic><topic>Coronavirus Infections - immunology</topic><topic>Coronavirus Infections - prevention & control</topic><topic>Coronavirus Infections - virology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitopes - immunology</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunity, Humoral - immunology</topic><topic>Infectious bronchitis virus</topic><topic>Infectious bronchitis virus - immunology</topic><topic>Infectious bronchitis virus - physiology</topic><topic>Leukocytes, Mononuclear - cytology</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>multi-epitope</topic><topic>peptide vaccine</topic><topic>Vaccines, Subunit - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YANG, Tai</creatorcontrib><creatorcontrib>WANG, Hong-Ning</creatorcontrib><creatorcontrib>WANG, Xue</creatorcontrib><creatorcontrib>TANG, Jun-Ni</creatorcontrib><creatorcontrib>LU, Dan</creatorcontrib><creatorcontrib>ZHANG, Yun-Fei</creatorcontrib><creatorcontrib>GUO, Zi-Cheng</creatorcontrib><creatorcontrib>LI, Yu-Ling</creatorcontrib><creatorcontrib>GAO, Rong</creatorcontrib><creatorcontrib>KANG, Run-Min</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YANG, Tai</au><au>WANG, Hong-Ning</au><au>WANG, Xue</au><au>TANG, Jun-Ni</au><au>LU, Dan</au><au>ZHANG, Yun-Fei</au><au>GUO, Zi-Cheng</au><au>LI, Yu-Ling</au><au>GAO, Rong</au><au>KANG, Run-Min</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Protective Immune Response against Infectious Bronchitis Virus Induced by Multi-Epitope Based Peptide Vaccines</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2009-07-01</date><risdate>2009</risdate><volume>73</volume><issue>7</issue><spage>1500</spage><epage>1504</epage><pages>1500-1504</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>Peptide vaccine was found to be an effective and powerful approach to a variety of pathogens. 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| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2009-07, Vol.73 (7), p.1500-1504 |
| issn | 0916-8451 1347-6947 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Animals Antibodies - analysis Antibodies - immunology Antigens, Viral - chemistry Antigens, Viral - immunology Biological and medical sciences Blotting, Western Cell Proliferation Chickens - immunology Chickens - virology Coronavirus Infections - immunology Coronavirus Infections - prevention & control Coronavirus Infections - virology Enzyme-Linked Immunosorbent Assay Epitopes - immunology Escherichia coli Fundamental and applied biological sciences. Psychology Immunity, Humoral - immunology Infectious bronchitis virus Infectious bronchitis virus - immunology Infectious bronchitis virus - physiology Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - immunology multi-epitope peptide vaccine Vaccines, Subunit - immunology |
| title | The Protective Immune Response against Infectious Bronchitis Virus Induced by Multi-Epitope Based Peptide Vaccines |
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