Diagnostic value of signal peptide‐CUB‐EGF domain‐containing protein 1 as an early and late biochemical marker in the ovarian torsion rat model
Aims Signal peptide‐CUB‐EGF (epidermal growth factor‐like protein) domain‐containing protein 1 (SCUBE1) is an experimental marker of ischemia that has been previously studied both in rat models and humans. In this study, we aim to investigate the importance of SCUBE1 levels in ovarian torsion using...
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| Veröffentlicht in: | The journal of obstetrics and gynaecology research 2018-06, Vol.44 (6), p.1092-1099 |
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| container_title | The journal of obstetrics and gynaecology research |
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| creator | Uzun, Özgür Kaban, Işık Midi, Ahmet Uysal, Hande Boran, Ahmet B. Bacanakgil, Besim H. Tarbaghia, Marwa |
| description | Aims
Signal peptide‐CUB‐EGF (epidermal growth factor‐like protein) domain‐containing protein 1 (SCUBE1) is an experimental marker of ischemia that has been previously studied both in rat models and humans. In this study, we aim to investigate the importance of SCUBE1 levels in ovarian torsion using an ovarian torsion model in rats.
Methods
A total of 18 Sprague–Dawley rats were equally divided into three groups. Group 1 (n = 6) was the Sham group and was only given a laparotomy procedure. Group 2 (n = 6) underwent bilateral ovarian torsion and ovarian ischemia lasting 8 h. Group 3 (n = 6) was subjected to bilateral ovarian torsion and ischemia lasting 24 h. Blood samples were collected from all three groups after the operations, and SCUBE1 levels were studied. Ovarian samples were collected, and microscopic evaluation was performed. The correlation of SCUBE1 levels and histopathological findings were investigated.
Results
The mean SCUBE1 level of group 3 was statistically higher than other groups (P < 0.01). Follicular degeneration and infiltration of inflammatory cells were, respectively, statistically significant in groups 2 and 3 (P = 0.002 and P = 0.045, respectively).
Conclusion
SCUBE1 can be useful in diagnosing ovarian torsion during the first 24 h, but more randomized controlled studies are necessary in order to implement it in clinical settings. |
| doi_str_mv | 10.1111/jog.13630 |
| format | Article |
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Signal peptide‐CUB‐EGF (epidermal growth factor‐like protein) domain‐containing protein 1 (SCUBE1) is an experimental marker of ischemia that has been previously studied both in rat models and humans. In this study, we aim to investigate the importance of SCUBE1 levels in ovarian torsion using an ovarian torsion model in rats.
Methods
A total of 18 Sprague–Dawley rats were equally divided into three groups. Group 1 (n = 6) was the Sham group and was only given a laparotomy procedure. Group 2 (n = 6) underwent bilateral ovarian torsion and ovarian ischemia lasting 8 h. Group 3 (n = 6) was subjected to bilateral ovarian torsion and ischemia lasting 24 h. Blood samples were collected from all three groups after the operations, and SCUBE1 levels were studied. Ovarian samples were collected, and microscopic evaluation was performed. The correlation of SCUBE1 levels and histopathological findings were investigated.
Results
The mean SCUBE1 level of group 3 was statistically higher than other groups (P < 0.01). Follicular degeneration and infiltration of inflammatory cells were, respectively, statistically significant in groups 2 and 3 (P = 0.002 and P = 0.045, respectively).
Conclusion
SCUBE1 can be useful in diagnosing ovarian torsion during the first 24 h, but more randomized controlled studies are necessary in order to implement it in clinical settings.</description><identifier>ISSN: 1341-8076</identifier><identifier>EISSN: 1447-0756</identifier><identifier>DOI: 10.1111/jog.13630</identifier><identifier>PMID: 29607598</identifier><language>eng</language><publisher>Kyoto, Japan: John Wiley & Sons Australia, Ltd</publisher><subject>Animal models ; Biochemical markers ; Degeneration ; Epidermal growth factor ; Inflammation ; Ischemia ; marker ; ovarian torsion ; Proteins ; rat ; Rodents ; signal peptide‐CUB‐EGF domain‐containing protein 1 ; Statistical analysis</subject><ispartof>The journal of obstetrics and gynaecology research, 2018-06, Vol.44 (6), p.1092-1099</ispartof><rights>2018 Japan Society of Obstetrics and Gynecology</rights><rights>2018 Japan Society of Obstetrics and Gynecology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3370-16169faa6d026313788c750319d59e27fe8dd3da43a8e0e295a90eec870385a43</cites><orcidid>0000-0002-6197-7654 ; 0000-0001-5134-0513</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjog.13630$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjog.13630$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29607598$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Uzun, Özgür</creatorcontrib><creatorcontrib>Kaban, Işık</creatorcontrib><creatorcontrib>Midi, Ahmet</creatorcontrib><creatorcontrib>Uysal, Hande</creatorcontrib><creatorcontrib>Boran, Ahmet B.</creatorcontrib><creatorcontrib>Bacanakgil, Besim H.</creatorcontrib><creatorcontrib>Tarbaghia, Marwa</creatorcontrib><title>Diagnostic value of signal peptide‐CUB‐EGF domain‐containing protein 1 as an early and late biochemical marker in the ovarian torsion rat model</title><title>The journal of obstetrics and gynaecology research</title><addtitle>J Obstet Gynaecol Res</addtitle><description>Aims
Signal peptide‐CUB‐EGF (epidermal growth factor‐like protein) domain‐containing protein 1 (SCUBE1) is an experimental marker of ischemia that has been previously studied both in rat models and humans. In this study, we aim to investigate the importance of SCUBE1 levels in ovarian torsion using an ovarian torsion model in rats.
Methods
A total of 18 Sprague–Dawley rats were equally divided into three groups. Group 1 (n = 6) was the Sham group and was only given a laparotomy procedure. Group 2 (n = 6) underwent bilateral ovarian torsion and ovarian ischemia lasting 8 h. Group 3 (n = 6) was subjected to bilateral ovarian torsion and ischemia lasting 24 h. Blood samples were collected from all three groups after the operations, and SCUBE1 levels were studied. Ovarian samples were collected, and microscopic evaluation was performed. The correlation of SCUBE1 levels and histopathological findings were investigated.
Results
The mean SCUBE1 level of group 3 was statistically higher than other groups (P < 0.01). Follicular degeneration and infiltration of inflammatory cells were, respectively, statistically significant in groups 2 and 3 (P = 0.002 and P = 0.045, respectively).
Conclusion
SCUBE1 can be useful in diagnosing ovarian torsion during the first 24 h, but more randomized controlled studies are necessary in order to implement it in clinical settings.</description><subject>Animal models</subject><subject>Biochemical markers</subject><subject>Degeneration</subject><subject>Epidermal growth factor</subject><subject>Inflammation</subject><subject>Ischemia</subject><subject>marker</subject><subject>ovarian torsion</subject><subject>Proteins</subject><subject>rat</subject><subject>Rodents</subject><subject>signal peptide‐CUB‐EGF domain‐containing protein 1</subject><subject>Statistical analysis</subject><issn>1341-8076</issn><issn>1447-0756</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kc1u1DAUhS0EoqWw4AWQJTawSGvHiX-WMLQDqFI3dB259k3qIYkH2ymaHY_AhhfkSbjDFBZIeGEfW989V9eHkOecnXJcZ5s4nHIhBXtAjnnTqIqpVj5ELRpeaabkEXmS84YxrgzXj8lRbSQiRh-TH--CHeaYS3D0zo4L0NjTHIbZjnQL2xI8_Pz2fXX9Fvfz9QX1cbJhxouLc0EV5oFuUywQZsqpzdTOFGwadyg8HW0BehOiu4UpOLScbPoMiSJcbrHVnU0BC0pMOcSZJlvoFD2MT8mj3o4Znt2fJ-T64vzT6n11ebX-sHpzWTkhFKu45NL01krPaim4UFo71TLBjW8N1KoH7b3wthFWA4PatNYwAKcVE7rF5xPy6uCLI3xZIJduCtnBONoZ4pK7mtVMm7oxEtGX_6CbuCT8pj3VKq6ahu-p1wfKpZhzgr7bpoBD7zrOun1WWDV0v7NC9sW943Izgf9L_gkHgbMD8DWMsPu_U_fxan2w_AVgKqCL</recordid><startdate>201806</startdate><enddate>201806</enddate><creator>Uzun, Özgür</creator><creator>Kaban, Işık</creator><creator>Midi, Ahmet</creator><creator>Uysal, Hande</creator><creator>Boran, Ahmet B.</creator><creator>Bacanakgil, Besim H.</creator><creator>Tarbaghia, Marwa</creator><general>John Wiley & Sons Australia, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TO</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6197-7654</orcidid><orcidid>https://orcid.org/0000-0001-5134-0513</orcidid></search><sort><creationdate>201806</creationdate><title>Diagnostic value of signal peptide‐CUB‐EGF domain‐containing protein 1 as an early and late biochemical marker in the ovarian torsion rat model</title><author>Uzun, Özgür ; Kaban, Işık ; Midi, Ahmet ; Uysal, Hande ; Boran, Ahmet B. ; Bacanakgil, Besim H. ; Tarbaghia, Marwa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3370-16169faa6d026313788c750319d59e27fe8dd3da43a8e0e295a90eec870385a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animal models</topic><topic>Biochemical markers</topic><topic>Degeneration</topic><topic>Epidermal growth factor</topic><topic>Inflammation</topic><topic>Ischemia</topic><topic>marker</topic><topic>ovarian torsion</topic><topic>Proteins</topic><topic>rat</topic><topic>Rodents</topic><topic>signal peptide‐CUB‐EGF domain‐containing protein 1</topic><topic>Statistical analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Uzun, Özgür</creatorcontrib><creatorcontrib>Kaban, Işık</creatorcontrib><creatorcontrib>Midi, Ahmet</creatorcontrib><creatorcontrib>Uysal, Hande</creatorcontrib><creatorcontrib>Boran, Ahmet B.</creatorcontrib><creatorcontrib>Bacanakgil, Besim H.</creatorcontrib><creatorcontrib>Tarbaghia, Marwa</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of obstetrics and gynaecology research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Uzun, Özgür</au><au>Kaban, Işık</au><au>Midi, Ahmet</au><au>Uysal, Hande</au><au>Boran, Ahmet B.</au><au>Bacanakgil, Besim H.</au><au>Tarbaghia, Marwa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diagnostic value of signal peptide‐CUB‐EGF domain‐containing protein 1 as an early and late biochemical marker in the ovarian torsion rat model</atitle><jtitle>The journal of obstetrics and gynaecology research</jtitle><addtitle>J Obstet Gynaecol Res</addtitle><date>2018-06</date><risdate>2018</risdate><volume>44</volume><issue>6</issue><spage>1092</spage><epage>1099</epage><pages>1092-1099</pages><issn>1341-8076</issn><eissn>1447-0756</eissn><abstract>Aims
Signal peptide‐CUB‐EGF (epidermal growth factor‐like protein) domain‐containing protein 1 (SCUBE1) is an experimental marker of ischemia that has been previously studied both in rat models and humans. In this study, we aim to investigate the importance of SCUBE1 levels in ovarian torsion using an ovarian torsion model in rats.
Methods
A total of 18 Sprague–Dawley rats were equally divided into three groups. Group 1 (n = 6) was the Sham group and was only given a laparotomy procedure. Group 2 (n = 6) underwent bilateral ovarian torsion and ovarian ischemia lasting 8 h. Group 3 (n = 6) was subjected to bilateral ovarian torsion and ischemia lasting 24 h. Blood samples were collected from all three groups after the operations, and SCUBE1 levels were studied. Ovarian samples were collected, and microscopic evaluation was performed. The correlation of SCUBE1 levels and histopathological findings were investigated.
Results
The mean SCUBE1 level of group 3 was statistically higher than other groups (P < 0.01). Follicular degeneration and infiltration of inflammatory cells were, respectively, statistically significant in groups 2 and 3 (P = 0.002 and P = 0.045, respectively).
Conclusion
SCUBE1 can be useful in diagnosing ovarian torsion during the first 24 h, but more randomized controlled studies are necessary in order to implement it in clinical settings.</abstract><cop>Kyoto, Japan</cop><pub>John Wiley & Sons Australia, Ltd</pub><pmid>29607598</pmid><doi>10.1111/jog.13630</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-6197-7654</orcidid><orcidid>https://orcid.org/0000-0001-5134-0513</orcidid></addata></record> |
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| subjects | Animal models Biochemical markers Degeneration Epidermal growth factor Inflammation Ischemia marker ovarian torsion Proteins rat Rodents signal peptide‐CUB‐EGF domain‐containing protein 1 Statistical analysis |
| title | Diagnostic value of signal peptide‐CUB‐EGF domain‐containing protein 1 as an early and late biochemical marker in the ovarian torsion rat model |
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