Towards the identification of alkaline phosphatase binding ligands in Li-Dan-Hua-Shi pills: A Box-Behnken design optimized affinity selection approach tandem with UHPLC-Q-TOF/MS analysis
•Magnetic alkaline phosphatase linked microspheres were fabricated and characterized.•Excellent magnetic behavior of microspheres resulting to its easy collection.•Magnetic affinity selection approach for fishing alkaline phosphatase binding ligands in herbal mixtures. Alkaline phosphatase conjugate...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2018-05, Vol.154, p.486-491 |
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| container_title | Journal of pharmaceutical and biomedical analysis |
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| creator | Tao, Yi Huang, Surun Gu, Xianghui Li, Weidong Cai, Baochang |
| description | •Magnetic alkaline phosphatase linked microspheres were fabricated and characterized.•Excellent magnetic behavior of microspheres resulting to its easy collection.•Magnetic affinity selection approach for fishing alkaline phosphatase binding ligands in herbal mixtures.
Alkaline phosphatase conjugated magnetic microspheres were synthesized via amide reaction, and employed as an effective adsorbent in affinity selection of binding ligands followed by UHPLC-Q-TOF/MS analysis. The analytical validity of the developed approach was evaluated under optimized conditions and the following figures of merit were obtained: linearity, 0.01–0.5 g L−1 with good determination coefficients (R2 = 0.9992); limits of detection (LODs), 0.003 g L−1; and limits of quantitation (LOQ), 0.01 g L−1. The precision (RSD%) of the proposed affinity selection approach was studied based on intra-day (0.8%) and inter-day (1.3%) precisions. Finally, the adsorbent was successfully applied to identification of binding ligands in Li-Dan-Hua-Shi pills and good recoveries were obtained in the range from 96.9 to 99.4% (RSDs 1.6–3.0%). |
| doi_str_mv | 10.1016/j.jpba.2018.03.041 |
| format | Article |
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Alkaline phosphatase conjugated magnetic microspheres were synthesized via amide reaction, and employed as an effective adsorbent in affinity selection of binding ligands followed by UHPLC-Q-TOF/MS analysis. The analytical validity of the developed approach was evaluated under optimized conditions and the following figures of merit were obtained: linearity, 0.01–0.5 g L−1 with good determination coefficients (R2 = 0.9992); limits of detection (LODs), 0.003 g L−1; and limits of quantitation (LOQ), 0.01 g L−1. The precision (RSD%) of the proposed affinity selection approach was studied based on intra-day (0.8%) and inter-day (1.3%) precisions. Finally, the adsorbent was successfully applied to identification of binding ligands in Li-Dan-Hua-Shi pills and good recoveries were obtained in the range from 96.9 to 99.4% (RSDs 1.6–3.0%).</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2018.03.041</identifier><identifier>PMID: 29602085</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Affinity selection ; Alkaline phosphatase ; Alkaline Phosphatase - chemistry ; Chromatography, High Pressure Liquid - methods ; Drugs, Chinese Herbal - chemistry ; Li-Dan-Hua-Shi pills ; Ligands ; Limit of Detection ; Magnetic microspheres ; Magnetics ; Reproducibility of Results ; Tandem Mass Spectrometry - methods</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2018-05, Vol.154, p.486-491</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-e26ead8922fd2600b4e6b6f7bf41643a85280378b53606a697e9cc4e8b5391193</citedby><cites>FETCH-LOGICAL-c356t-e26ead8922fd2600b4e6b6f7bf41643a85280378b53606a697e9cc4e8b5391193</cites><orcidid>0000-0001-8773-8443</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jpba.2018.03.041$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29602085$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tao, Yi</creatorcontrib><creatorcontrib>Huang, Surun</creatorcontrib><creatorcontrib>Gu, Xianghui</creatorcontrib><creatorcontrib>Li, Weidong</creatorcontrib><creatorcontrib>Cai, Baochang</creatorcontrib><title>Towards the identification of alkaline phosphatase binding ligands in Li-Dan-Hua-Shi pills: A Box-Behnken design optimized affinity selection approach tandem with UHPLC-Q-TOF/MS analysis</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>•Magnetic alkaline phosphatase linked microspheres were fabricated and characterized.•Excellent magnetic behavior of microspheres resulting to its easy collection.•Magnetic affinity selection approach for fishing alkaline phosphatase binding ligands in herbal mixtures.
Alkaline phosphatase conjugated magnetic microspheres were synthesized via amide reaction, and employed as an effective adsorbent in affinity selection of binding ligands followed by UHPLC-Q-TOF/MS analysis. The analytical validity of the developed approach was evaluated under optimized conditions and the following figures of merit were obtained: linearity, 0.01–0.5 g L−1 with good determination coefficients (R2 = 0.9992); limits of detection (LODs), 0.003 g L−1; and limits of quantitation (LOQ), 0.01 g L−1. The precision (RSD%) of the proposed affinity selection approach was studied based on intra-day (0.8%) and inter-day (1.3%) precisions. Finally, the adsorbent was successfully applied to identification of binding ligands in Li-Dan-Hua-Shi pills and good recoveries were obtained in the range from 96.9 to 99.4% (RSDs 1.6–3.0%).</description><subject>Affinity selection</subject><subject>Alkaline phosphatase</subject><subject>Alkaline Phosphatase - chemistry</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Drugs, Chinese Herbal - chemistry</subject><subject>Li-Dan-Hua-Shi pills</subject><subject>Ligands</subject><subject>Limit of Detection</subject><subject>Magnetic microspheres</subject><subject>Magnetics</subject><subject>Reproducibility of Results</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhiMEotvCC3BAPnJxajuJkyAu7UJZpEUFdStxsybJZDNbrxPiLO3yaDwdXrZw5GRp9P-f7fmi6JUUsRRSn2_izVBBrIQsYpHEIpVPopks8oQrnX57Gs1EnkieiyI7iU693wghMlmmz6MTVWqhwnwW_Vr19zA2nk0dMmrQTdRSDRP1jvUtA3sHlhyyoev90MEEHllFriG3ZpbW4EKVHFsSfw-OL3bAbzpiA1nr37ILdtk_8Evs3B061qCndaAOE23pJzYM2pYcTXvm0WL950oYhrGHumNTIOOW3dPUsdvFl-Wcf-Wr66vzzzcMHNi9J_8ietaC9fjy8TyLbq8-rOYLvrz--Gl-seR1kumJo9IITVEq1TZKC1GlqCvd5lWbSp0mUGSqEEleVFmihQZd5ljWdYqHQSllmZxFb47c8LTvO_ST2ZKv0Vpw2O-8UWGVacAnOkTVMVqPvfcjtmYYaQvj3khhDs7MxhycmYMzIxITnIXS60f-rtpi86_yV1IIvDsGMPzyB-FofE3oamxoDHszTU__4_8GYA6psA</recordid><startdate>20180530</startdate><enddate>20180530</enddate><creator>Tao, Yi</creator><creator>Huang, Surun</creator><creator>Gu, Xianghui</creator><creator>Li, Weidong</creator><creator>Cai, Baochang</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8773-8443</orcidid></search><sort><creationdate>20180530</creationdate><title>Towards the identification of alkaline phosphatase binding ligands in Li-Dan-Hua-Shi pills: A Box-Behnken design optimized affinity selection approach tandem with UHPLC-Q-TOF/MS analysis</title><author>Tao, Yi ; Huang, Surun ; Gu, Xianghui ; Li, Weidong ; Cai, Baochang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-e26ead8922fd2600b4e6b6f7bf41643a85280378b53606a697e9cc4e8b5391193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Affinity selection</topic><topic>Alkaline phosphatase</topic><topic>Alkaline Phosphatase - chemistry</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Drugs, Chinese Herbal - chemistry</topic><topic>Li-Dan-Hua-Shi pills</topic><topic>Ligands</topic><topic>Limit of Detection</topic><topic>Magnetic microspheres</topic><topic>Magnetics</topic><topic>Reproducibility of Results</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tao, Yi</creatorcontrib><creatorcontrib>Huang, Surun</creatorcontrib><creatorcontrib>Gu, Xianghui</creatorcontrib><creatorcontrib>Li, Weidong</creatorcontrib><creatorcontrib>Cai, Baochang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tao, Yi</au><au>Huang, Surun</au><au>Gu, Xianghui</au><au>Li, Weidong</au><au>Cai, Baochang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Towards the identification of alkaline phosphatase binding ligands in Li-Dan-Hua-Shi pills: A Box-Behnken design optimized affinity selection approach tandem with UHPLC-Q-TOF/MS analysis</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2018-05-30</date><risdate>2018</risdate><volume>154</volume><spage>486</spage><epage>491</epage><pages>486-491</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><abstract>•Magnetic alkaline phosphatase linked microspheres were fabricated and characterized.•Excellent magnetic behavior of microspheres resulting to its easy collection.•Magnetic affinity selection approach for fishing alkaline phosphatase binding ligands in herbal mixtures.
Alkaline phosphatase conjugated magnetic microspheres were synthesized via amide reaction, and employed as an effective adsorbent in affinity selection of binding ligands followed by UHPLC-Q-TOF/MS analysis. The analytical validity of the developed approach was evaluated under optimized conditions and the following figures of merit were obtained: linearity, 0.01–0.5 g L−1 with good determination coefficients (R2 = 0.9992); limits of detection (LODs), 0.003 g L−1; and limits of quantitation (LOQ), 0.01 g L−1. The precision (RSD%) of the proposed affinity selection approach was studied based on intra-day (0.8%) and inter-day (1.3%) precisions. Finally, the adsorbent was successfully applied to identification of binding ligands in Li-Dan-Hua-Shi pills and good recoveries were obtained in the range from 96.9 to 99.4% (RSDs 1.6–3.0%).</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>29602085</pmid><doi>10.1016/j.jpba.2018.03.041</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-8773-8443</orcidid></addata></record> |
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| subjects | Affinity selection Alkaline phosphatase Alkaline Phosphatase - chemistry Chromatography, High Pressure Liquid - methods Drugs, Chinese Herbal - chemistry Li-Dan-Hua-Shi pills Ligands Limit of Detection Magnetic microspheres Magnetics Reproducibility of Results Tandem Mass Spectrometry - methods |
| title | Towards the identification of alkaline phosphatase binding ligands in Li-Dan-Hua-Shi pills: A Box-Behnken design optimized affinity selection approach tandem with UHPLC-Q-TOF/MS analysis |
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