Identification of Separate Structural Features That Affect Rate and Cation Concentration Dependence of Self-Cleavage by the Neurospora VS Ribozyme

The cleavage site of the Neurospora VS ribozyme is located in an internal loop in a hairpin called stem-loop I. Stem-loop I undergoes a cation-dependent structural change to adopt a conformation, termed shifted, that is required for activity. Using site-directed mutagenesis and kinetic analyses, we...

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Veröffentlicht in:	Biochemistry (Easton) 2006-11, Vol.45 (44), p.13394-13400
Hauptverfasser:	Poon, Alan H. L, Olive, Joan E, McLaren, Meredith, Collins, Richard A
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Cations Hydrolysis Kinetics Magnesium - metabolism Mutagenesis, Site-Directed Neurospora Neurospora - enzymology Nucleic Acid Conformation Protein Conformation RNA, Catalytic - chemistry RNA, Catalytic - genetics RNA, Catalytic - metabolism
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