The FlaG regulator is involved in length control of the polar flagella of Campylobacter jejuni
Campylobacter jejuni cells have bipolar flagella. Both flagella have similar lengths of about one helical turn, or 3.53±0.52 µm. The flagellar filament is composed of two homologous flagellins: FlaA and FlaB. Mutant strains that express either FlaA or FlaB alone produce filaments that are shorter th...
Gespeichert in:
| Veröffentlicht in: | Microbiology (Society for General Microbiology) 2018-05, Vol.164 (5), p.740-750 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 750 |
|---|---|
| container_issue | 5 |
| container_start_page | 740 |
| container_title | Microbiology (Society for General Microbiology) |
| container_volume | 164 |
| creator | Inoue, Tomoharu Barker, Clive S Matsunami, Hideyuki Aizawa, Shin-Ichi Samatey, Fadel A |
| description | Campylobacter jejuni cells have bipolar flagella. Both flagella have similar lengths of about one helical turn, or 3.53±0.52 µm. The flagellar filament is composed of two homologous flagellins: FlaA and FlaB. Mutant strains that express either FlaA or FlaB alone produce filaments that are shorter than those of the wild-type. It is reported that the flaG gene could affect filament length in some species of bacteria, but its function remains unknown. We introduced a flaG-deletion mutation into the C. jejuni wild-type strain and flaA- or flaB-deletion mutant strains, and observed their flagella by microscopy. The ΔflaG mutant cells produced long filaments of two helical turns in the wild-type background. The ΔflaAG double mutant cells produced very short FlaB filaments. On the other hand, ΔflaBG double mutant cells produced long FlaA filaments and their morphology was not helical but straight. Furthermore, FlaG was secreted, and a pulldown assay showed that sigma factor 28 was co-precipitated with purified polyhistidine-tagged FlaG. We conclude that FlaG controls flagella length by negatively regulating FlaA filament assembly and discuss the role of FlaA and FlaB flagellins in C. jejuni flagella formation. |
| doi_str_mv | 10.1099/mic.0.000648 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2019810362</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2019810362</sourcerecordid><originalsourceid>FETCH-LOGICAL-c329t-406a07f568b54a3d3077d1438bfb3e7af1d6ccfc5ceca0c562a2bfd1be14b6613</originalsourceid><addsrcrecordid>eNo9kL1PwzAQxS0EoqWwMSOPDKScP-IkI6poQarEUlYi27HbVE4c7ASp_z2pWpju6e73nk4PoXsCcwJF8dzUeg5zABA8v0BTwkWaUMjhctQshQTyjE7QTYx7gPEI5BpNaJEWKSd8ir42O4OXTq5wMNvByd4HXEdctz_e_ZhqFNiZdtvvsPZtH7zD3uJ-9HTeyYCtk1vjnDxuF7LpDs4rqXsT8N7sh7a-RVdWumjuznOGPpevm8Vbsv5YvS9e1olmtOgTDkJCZlORq5RLVjHIsopwliurmMmkJZXQ2upUGy1Bp4JKqmxFlCFcCUHYDD2ecrvgvwcT-7Kpoz5-1ho_xJICKXICTNARfTqhOvgYg7FlF-pGhkNJoDw2Olp1CeWp0RF_OCcPqjHVP_xXIfsFNnByXQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2019810362</pqid></control><display><type>article</type><title>The FlaG regulator is involved in length control of the polar flagella of Campylobacter jejuni</title><source>MEDLINE</source><source>PubMed Central</source><creator>Inoue, Tomoharu ; Barker, Clive S ; Matsunami, Hideyuki ; Aizawa, Shin-Ichi ; Samatey, Fadel A</creator><creatorcontrib>Inoue, Tomoharu ; Barker, Clive S ; Matsunami, Hideyuki ; Aizawa, Shin-Ichi ; Samatey, Fadel A</creatorcontrib><description>Campylobacter jejuni cells have bipolar flagella. Both flagella have similar lengths of about one helical turn, or 3.53±0.52 µm. The flagellar filament is composed of two homologous flagellins: FlaA and FlaB. Mutant strains that express either FlaA or FlaB alone produce filaments that are shorter than those of the wild-type. It is reported that the flaG gene could affect filament length in some species of bacteria, but its function remains unknown. We introduced a flaG-deletion mutation into the C. jejuni wild-type strain and flaA- or flaB-deletion mutant strains, and observed their flagella by microscopy. The ΔflaG mutant cells produced long filaments of two helical turns in the wild-type background. The ΔflaAG double mutant cells produced very short FlaB filaments. On the other hand, ΔflaBG double mutant cells produced long FlaA filaments and their morphology was not helical but straight. Furthermore, FlaG was secreted, and a pulldown assay showed that sigma factor 28 was co-precipitated with purified polyhistidine-tagged FlaG. We conclude that FlaG controls flagella length by negatively regulating FlaA filament assembly and discuss the role of FlaA and FlaB flagellins in C. jejuni flagella formation.</description><identifier>ISSN: 1350-0872</identifier><identifier>EISSN: 1465-2080</identifier><identifier>DOI: 10.1099/mic.0.000648</identifier><identifier>PMID: 29595414</identifier><language>eng</language><publisher>England</publisher><subject>Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Campylobacter jejuni - cytology ; Campylobacter jejuni - genetics ; Campylobacter jejuni - metabolism ; Campylobacter jejuni - physiology ; Flagella - genetics ; Flagella - metabolism ; Flagella - ultrastructure ; Flagellin - genetics ; Flagellin - metabolism ; Gene Deletion ; Gene Expression Regulation, Bacterial ; Locomotion ; Microscopy, Electron, Transmission ; Protein Binding ; Sigma Factor - metabolism</subject><ispartof>Microbiology (Society for General Microbiology), 2018-05, Vol.164 (5), p.740-750</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c329t-406a07f568b54a3d3077d1438bfb3e7af1d6ccfc5ceca0c562a2bfd1be14b6613</citedby><cites>FETCH-LOGICAL-c329t-406a07f568b54a3d3077d1438bfb3e7af1d6ccfc5ceca0c562a2bfd1be14b6613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29595414$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inoue, Tomoharu</creatorcontrib><creatorcontrib>Barker, Clive S</creatorcontrib><creatorcontrib>Matsunami, Hideyuki</creatorcontrib><creatorcontrib>Aizawa, Shin-Ichi</creatorcontrib><creatorcontrib>Samatey, Fadel A</creatorcontrib><title>The FlaG regulator is involved in length control of the polar flagella of Campylobacter jejuni</title><title>Microbiology (Society for General Microbiology)</title><addtitle>Microbiology</addtitle><description>Campylobacter jejuni cells have bipolar flagella. Both flagella have similar lengths of about one helical turn, or 3.53±0.52 µm. The flagellar filament is composed of two homologous flagellins: FlaA and FlaB. Mutant strains that express either FlaA or FlaB alone produce filaments that are shorter than those of the wild-type. It is reported that the flaG gene could affect filament length in some species of bacteria, but its function remains unknown. We introduced a flaG-deletion mutation into the C. jejuni wild-type strain and flaA- or flaB-deletion mutant strains, and observed their flagella by microscopy. The ΔflaG mutant cells produced long filaments of two helical turns in the wild-type background. The ΔflaAG double mutant cells produced very short FlaB filaments. On the other hand, ΔflaBG double mutant cells produced long FlaA filaments and their morphology was not helical but straight. Furthermore, FlaG was secreted, and a pulldown assay showed that sigma factor 28 was co-precipitated with purified polyhistidine-tagged FlaG. We conclude that FlaG controls flagella length by negatively regulating FlaA filament assembly and discuss the role of FlaA and FlaB flagellins in C. jejuni flagella formation.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Campylobacter jejuni - cytology</subject><subject>Campylobacter jejuni - genetics</subject><subject>Campylobacter jejuni - metabolism</subject><subject>Campylobacter jejuni - physiology</subject><subject>Flagella - genetics</subject><subject>Flagella - metabolism</subject><subject>Flagella - ultrastructure</subject><subject>Flagellin - genetics</subject><subject>Flagellin - metabolism</subject><subject>Gene Deletion</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Locomotion</subject><subject>Microscopy, Electron, Transmission</subject><subject>Protein Binding</subject><subject>Sigma Factor - metabolism</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kL1PwzAQxS0EoqWwMSOPDKScP-IkI6poQarEUlYi27HbVE4c7ASp_z2pWpju6e73nk4PoXsCcwJF8dzUeg5zABA8v0BTwkWaUMjhctQshQTyjE7QTYx7gPEI5BpNaJEWKSd8ir42O4OXTq5wMNvByd4HXEdctz_e_ZhqFNiZdtvvsPZtH7zD3uJ-9HTeyYCtk1vjnDxuF7LpDs4rqXsT8N7sh7a-RVdWumjuznOGPpevm8Vbsv5YvS9e1olmtOgTDkJCZlORq5RLVjHIsopwliurmMmkJZXQ2upUGy1Bp4JKqmxFlCFcCUHYDD2ecrvgvwcT-7Kpoz5-1ho_xJICKXICTNARfTqhOvgYg7FlF-pGhkNJoDw2Olp1CeWp0RF_OCcPqjHVP_xXIfsFNnByXQ</recordid><startdate>201805</startdate><enddate>201805</enddate><creator>Inoue, Tomoharu</creator><creator>Barker, Clive S</creator><creator>Matsunami, Hideyuki</creator><creator>Aizawa, Shin-Ichi</creator><creator>Samatey, Fadel A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201805</creationdate><title>The FlaG regulator is involved in length control of the polar flagella of Campylobacter jejuni</title><author>Inoue, Tomoharu ; Barker, Clive S ; Matsunami, Hideyuki ; Aizawa, Shin-Ichi ; Samatey, Fadel A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c329t-406a07f568b54a3d3077d1438bfb3e7af1d6ccfc5ceca0c562a2bfd1be14b6613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Campylobacter jejuni - cytology</topic><topic>Campylobacter jejuni - genetics</topic><topic>Campylobacter jejuni - metabolism</topic><topic>Campylobacter jejuni - physiology</topic><topic>Flagella - genetics</topic><topic>Flagella - metabolism</topic><topic>Flagella - ultrastructure</topic><topic>Flagellin - genetics</topic><topic>Flagellin - metabolism</topic><topic>Gene Deletion</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Locomotion</topic><topic>Microscopy, Electron, Transmission</topic><topic>Protein Binding</topic><topic>Sigma Factor - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inoue, Tomoharu</creatorcontrib><creatorcontrib>Barker, Clive S</creatorcontrib><creatorcontrib>Matsunami, Hideyuki</creatorcontrib><creatorcontrib>Aizawa, Shin-Ichi</creatorcontrib><creatorcontrib>Samatey, Fadel A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inoue, Tomoharu</au><au>Barker, Clive S</au><au>Matsunami, Hideyuki</au><au>Aizawa, Shin-Ichi</au><au>Samatey, Fadel A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The FlaG regulator is involved in length control of the polar flagella of Campylobacter jejuni</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>2018-05</date><risdate>2018</risdate><volume>164</volume><issue>5</issue><spage>740</spage><epage>750</epage><pages>740-750</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>Campylobacter jejuni cells have bipolar flagella. Both flagella have similar lengths of about one helical turn, or 3.53±0.52 µm. The flagellar filament is composed of two homologous flagellins: FlaA and FlaB. Mutant strains that express either FlaA or FlaB alone produce filaments that are shorter than those of the wild-type. It is reported that the flaG gene could affect filament length in some species of bacteria, but its function remains unknown. We introduced a flaG-deletion mutation into the C. jejuni wild-type strain and flaA- or flaB-deletion mutant strains, and observed their flagella by microscopy. The ΔflaG mutant cells produced long filaments of two helical turns in the wild-type background. The ΔflaAG double mutant cells produced very short FlaB filaments. On the other hand, ΔflaBG double mutant cells produced long FlaA filaments and their morphology was not helical but straight. Furthermore, FlaG was secreted, and a pulldown assay showed that sigma factor 28 was co-precipitated with purified polyhistidine-tagged FlaG. We conclude that FlaG controls flagella length by negatively regulating FlaA filament assembly and discuss the role of FlaA and FlaB flagellins in C. jejuni flagella formation.</abstract><cop>England</cop><pmid>29595414</pmid><doi>10.1099/mic.0.000648</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1350-0872 |
| ispartof | Microbiology (Society for General Microbiology), 2018-05, Vol.164 (5), p.740-750 |
| issn | 1350-0872 1465-2080 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2019810362 |
| source | MEDLINE; PubMed Central |
| subjects | Bacterial Proteins - genetics Bacterial Proteins - metabolism Campylobacter jejuni - cytology Campylobacter jejuni - genetics Campylobacter jejuni - metabolism Campylobacter jejuni - physiology Flagella - genetics Flagella - metabolism Flagella - ultrastructure Flagellin - genetics Flagellin - metabolism Gene Deletion Gene Expression Regulation, Bacterial Locomotion Microscopy, Electron, Transmission Protein Binding Sigma Factor - metabolism |
| title | The FlaG regulator is involved in length control of the polar flagella of Campylobacter jejuni |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T19%3A55%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20FlaG%20regulator%20is%20involved%20in%20length%20control%20of%20the%20polar%20flagella%20of%20Campylobacter%20jejuni&rft.jtitle=Microbiology%20(Society%20for%20General%20Microbiology)&rft.au=Inoue,%20Tomoharu&rft.date=2018-05&rft.volume=164&rft.issue=5&rft.spage=740&rft.epage=750&rft.pages=740-750&rft.issn=1350-0872&rft.eissn=1465-2080&rft_id=info:doi/10.1099/mic.0.000648&rft_dat=%3Cproquest_cross%3E2019810362%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2019810362&rft_id=info:pmid/29595414&rfr_iscdi=true |