Demonstration of the lack of cytotoxicity of unmodified and folic acid modified graphene oxide quantum dots, and their application to fluorescence lifetime imaging of HaCaT cells
The authors describe the synthesis of water-soluble and fluorescent graphene oxide quantum dots via acid exfoliation of graphite nanoparticles. The resultant graphene oxide quantum dots (GoQDs) were then modified with folic acid. Folic acid receptors are overexpressed in cancer cells and hence can b...
Gespeichert in:
Veröffentlicht in: | Mikrochimica acta (1966) 2018-02, Vol.185 (2), p.128-7, Article 128 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 7 |
---|---|
container_issue | 2 |
container_start_page | 128 |
container_title | Mikrochimica acta (1966) |
container_volume | 185 |
creator | Goreham, Renee V. Schroeder, Kathryn L. Holmes, Amy Bradley, Siobhan J. Nann, Thomas |
description | The authors describe the synthesis of water-soluble and fluorescent graphene oxide quantum dots via acid exfoliation of graphite nanoparticles. The resultant graphene oxide quantum dots (GoQDs) were then modified with folic acid. Folic acid receptors are overexpressed in cancer cells and hence can bind to functionalized graphene oxide quantum dots. On excitation at 305 nm, the GoQDs display green fluorescence with a peak wavelength at ~520 nm. The modified GoQDs are non-toxic to macrophage cells even after prolonged exposure and high concentrations. Fluorescence lifetime imaging and multiphoton microscopy was used (in combination) to image HeCaT cells exposed to GoQDs, resulting in a superior method for bioimaging.
Graphical abstract
Schematic representation of graphene oxide quantum dots, folic acid modified graphene oxide quantum dots (red), and the use of fluorescence lifetime to discriminate against green auto-fluorescence of HeCaT cells. |
doi_str_mv | 10.1007/s00604-018-2679-8 |
format | Article |
fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2019810168</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A715058978</galeid><sourcerecordid>A715058978</sourcerecordid><originalsourceid>FETCH-LOGICAL-c439t-761917fc9961c1ef053dd6c96968bf965907b6766e6a59e26a572ae63e2001c83</originalsourceid><addsrcrecordid>eNp1Uk2P1SAUbYzGeY7-ADeGxI0LOwJtoSwnz48xmcTNuCY8eukwttABmvj-lr9QsOP4EQ0JXC7nnHu5OVX1nOAzgjF_EzFmuK0x6WvKuKj7B9WOtA2rO8ybh9UOY8rqhnF6Uj2J8QZjwhltH1cnVHSiZZzsqm9vYfYupqCS9Q55g9I1oEnpLyXWx-ST_2q1TcdyX93sB2ssDEi5ARk_WY2UtgO6z49BLdfgAGXaAOh2VS6tMxp8iq9_kLK-DUgtS-ZuRZNHZlp9gKjB6VzdGkh2BmRnNVo3lsoXaq-ukIZpik-rR0ZNEZ7dnafV5_fvrvYX9eWnDx_355e1bhuRas6IINxoIRjRBAzummFgWjDB-oMRrBOYHxhnDJjqBNC8c6qANUDzoHTfnFavNt0l-NsVYpKzjaUD5cCvUVJMRE8wYQX68i_ojV-Dy91JIkTTNpT05BdqVBNI64zPY9dFVJ5z0uGuF7xonf0DldcAs9XegbE5_weBbAQdfIwBjFxCnlw4SoJl8YncfCKzT2TxiSycF3cNr4cZhnvGT2NkAN0AMT-5EcJvP_qv6nd8fMjB</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1993432181</pqid></control><display><type>article</type><title>Demonstration of the lack of cytotoxicity of unmodified and folic acid modified graphene oxide quantum dots, and their application to fluorescence lifetime imaging of HaCaT cells</title><source>SpringerNature Journals</source><creator>Goreham, Renee V. ; Schroeder, Kathryn L. ; Holmes, Amy ; Bradley, Siobhan J. ; Nann, Thomas</creator><creatorcontrib>Goreham, Renee V. ; Schroeder, Kathryn L. ; Holmes, Amy ; Bradley, Siobhan J. ; Nann, Thomas</creatorcontrib><description>The authors describe the synthesis of water-soluble and fluorescent graphene oxide quantum dots via acid exfoliation of graphite nanoparticles. The resultant graphene oxide quantum dots (GoQDs) were then modified with folic acid. Folic acid receptors are overexpressed in cancer cells and hence can bind to functionalized graphene oxide quantum dots. On excitation at 305 nm, the GoQDs display green fluorescence with a peak wavelength at ~520 nm. The modified GoQDs are non-toxic to macrophage cells even after prolonged exposure and high concentrations. Fluorescence lifetime imaging and multiphoton microscopy was used (in combination) to image HeCaT cells exposed to GoQDs, resulting in a superior method for bioimaging.
Graphical abstract
Schematic representation of graphene oxide quantum dots, folic acid modified graphene oxide quantum dots (red), and the use of fluorescence lifetime to discriminate against green auto-fluorescence of HeCaT cells.</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-018-2679-8</identifier><identifier>PMID: 29594671</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Acids ; Analytical Chemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Fluorescence ; Folic acid ; Graphene ; Graphite ; Medical imaging ; Microengineering ; Nanochemistry ; Nanotechnology ; Original Paper ; Quantum dots ; Receptors ; Toxicity ; Vitamin B</subject><ispartof>Mikrochimica acta (1966), 2018-02, Vol.185 (2), p.128-7, Article 128</ispartof><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Microchimica Acta is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-761917fc9961c1ef053dd6c96968bf965907b6766e6a59e26a572ae63e2001c83</citedby><cites>FETCH-LOGICAL-c439t-761917fc9961c1ef053dd6c96968bf965907b6766e6a59e26a572ae63e2001c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-018-2679-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-018-2679-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29594671$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goreham, Renee V.</creatorcontrib><creatorcontrib>Schroeder, Kathryn L.</creatorcontrib><creatorcontrib>Holmes, Amy</creatorcontrib><creatorcontrib>Bradley, Siobhan J.</creatorcontrib><creatorcontrib>Nann, Thomas</creatorcontrib><title>Demonstration of the lack of cytotoxicity of unmodified and folic acid modified graphene oxide quantum dots, and their application to fluorescence lifetime imaging of HaCaT cells</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><addtitle>Mikrochim Acta</addtitle><description>The authors describe the synthesis of water-soluble and fluorescent graphene oxide quantum dots via acid exfoliation of graphite nanoparticles. The resultant graphene oxide quantum dots (GoQDs) were then modified with folic acid. Folic acid receptors are overexpressed in cancer cells and hence can bind to functionalized graphene oxide quantum dots. On excitation at 305 nm, the GoQDs display green fluorescence with a peak wavelength at ~520 nm. The modified GoQDs are non-toxic to macrophage cells even after prolonged exposure and high concentrations. Fluorescence lifetime imaging and multiphoton microscopy was used (in combination) to image HeCaT cells exposed to GoQDs, resulting in a superior method for bioimaging.
Graphical abstract
Schematic representation of graphene oxide quantum dots, folic acid modified graphene oxide quantum dots (red), and the use of fluorescence lifetime to discriminate against green auto-fluorescence of HeCaT cells.</description><subject>Acids</subject><subject>Analytical Chemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Fluorescence</subject><subject>Folic acid</subject><subject>Graphene</subject><subject>Graphite</subject><subject>Medical imaging</subject><subject>Microengineering</subject><subject>Nanochemistry</subject><subject>Nanotechnology</subject><subject>Original Paper</subject><subject>Quantum dots</subject><subject>Receptors</subject><subject>Toxicity</subject><subject>Vitamin B</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1Uk2P1SAUbYzGeY7-ADeGxI0LOwJtoSwnz48xmcTNuCY8eukwttABmvj-lr9QsOP4EQ0JXC7nnHu5OVX1nOAzgjF_EzFmuK0x6WvKuKj7B9WOtA2rO8ybh9UOY8rqhnF6Uj2J8QZjwhltH1cnVHSiZZzsqm9vYfYupqCS9Q55g9I1oEnpLyXWx-ST_2q1TcdyX93sB2ssDEi5ARk_WY2UtgO6z49BLdfgAGXaAOh2VS6tMxp8iq9_kLK-DUgtS-ZuRZNHZlp9gKjB6VzdGkh2BmRnNVo3lsoXaq-ukIZpik-rR0ZNEZ7dnafV5_fvrvYX9eWnDx_355e1bhuRas6IINxoIRjRBAzummFgWjDB-oMRrBOYHxhnDJjqBNC8c6qANUDzoHTfnFavNt0l-NsVYpKzjaUD5cCvUVJMRE8wYQX68i_ojV-Dy91JIkTTNpT05BdqVBNI64zPY9dFVJ5z0uGuF7xonf0DldcAs9XegbE5_weBbAQdfIwBjFxCnlw4SoJl8YncfCKzT2TxiSycF3cNr4cZhnvGT2NkAN0AMT-5EcJvP_qv6nd8fMjB</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>Goreham, Renee V.</creator><creator>Schroeder, Kathryn L.</creator><creator>Holmes, Amy</creator><creator>Bradley, Siobhan J.</creator><creator>Nann, Thomas</creator><general>Springer Vienna</general><general>Springer</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>M0S</scope><scope>M1P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20180201</creationdate><title>Demonstration of the lack of cytotoxicity of unmodified and folic acid modified graphene oxide quantum dots, and their application to fluorescence lifetime imaging of HaCaT cells</title><author>Goreham, Renee V. ; Schroeder, Kathryn L. ; Holmes, Amy ; Bradley, Siobhan J. ; Nann, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-761917fc9961c1ef053dd6c96968bf965907b6766e6a59e26a572ae63e2001c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acids</topic><topic>Analytical Chemistry</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Fluorescence</topic><topic>Folic acid</topic><topic>Graphene</topic><topic>Graphite</topic><topic>Medical imaging</topic><topic>Microengineering</topic><topic>Nanochemistry</topic><topic>Nanotechnology</topic><topic>Original Paper</topic><topic>Quantum dots</topic><topic>Receptors</topic><topic>Toxicity</topic><topic>Vitamin B</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goreham, Renee V.</creatorcontrib><creatorcontrib>Schroeder, Kathryn L.</creatorcontrib><creatorcontrib>Holmes, Amy</creatorcontrib><creatorcontrib>Bradley, Siobhan J.</creatorcontrib><creatorcontrib>Nann, Thomas</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goreham, Renee V.</au><au>Schroeder, Kathryn L.</au><au>Holmes, Amy</au><au>Bradley, Siobhan J.</au><au>Nann, Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Demonstration of the lack of cytotoxicity of unmodified and folic acid modified graphene oxide quantum dots, and their application to fluorescence lifetime imaging of HaCaT cells</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2018-02-01</date><risdate>2018</risdate><volume>185</volume><issue>2</issue><spage>128</spage><epage>7</epage><pages>128-7</pages><artnum>128</artnum><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>The authors describe the synthesis of water-soluble and fluorescent graphene oxide quantum dots via acid exfoliation of graphite nanoparticles. The resultant graphene oxide quantum dots (GoQDs) were then modified with folic acid. Folic acid receptors are overexpressed in cancer cells and hence can bind to functionalized graphene oxide quantum dots. On excitation at 305 nm, the GoQDs display green fluorescence with a peak wavelength at ~520 nm. The modified GoQDs are non-toxic to macrophage cells even after prolonged exposure and high concentrations. Fluorescence lifetime imaging and multiphoton microscopy was used (in combination) to image HeCaT cells exposed to GoQDs, resulting in a superior method for bioimaging.
Graphical abstract
Schematic representation of graphene oxide quantum dots, folic acid modified graphene oxide quantum dots (red), and the use of fluorescence lifetime to discriminate against green auto-fluorescence of HeCaT cells.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>29594671</pmid><doi>10.1007/s00604-018-2679-8</doi><tpages>7</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0026-3672 |
ispartof | Mikrochimica acta (1966), 2018-02, Vol.185 (2), p.128-7, Article 128 |
issn | 0026-3672 1436-5073 |
language | eng |
recordid | cdi_proquest_miscellaneous_2019810168 |
source | SpringerNature Journals |
subjects | Acids Analytical Chemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Fluorescence Folic acid Graphene Graphite Medical imaging Microengineering Nanochemistry Nanotechnology Original Paper Quantum dots Receptors Toxicity Vitamin B |
title | Demonstration of the lack of cytotoxicity of unmodified and folic acid modified graphene oxide quantum dots, and their application to fluorescence lifetime imaging of HaCaT cells |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-14T01%3A29%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Demonstration%20of%20the%20lack%20of%20cytotoxicity%20of%20unmodified%20and%20folic%20acid%20modified%20graphene%20oxide%20quantum%20dots,%20and%20their%20application%20to%20fluorescence%20lifetime%20imaging%20of%20HaCaT%20cells&rft.jtitle=Mikrochimica%20acta%20(1966)&rft.au=Goreham,%20Renee%20V.&rft.date=2018-02-01&rft.volume=185&rft.issue=2&rft.spage=128&rft.epage=7&rft.pages=128-7&rft.artnum=128&rft.issn=0026-3672&rft.eissn=1436-5073&rft_id=info:doi/10.1007/s00604-018-2679-8&rft_dat=%3Cgale_proqu%3EA715058978%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1993432181&rft_id=info:pmid/29594671&rft_galeid=A715058978&rfr_iscdi=true |