Polydopamine nanodots are viable probes for fluorometric determination of the activity of alkaline phosphatase via the in situ regulation of a redox reaction triggered by the enzyme
The authors describe an environmentally friendly and fast (~14 min) method for the synthesis of homogeneously distributed fluorescent polydopamine nanodots (PDA-NDs) using KMnO 4 as the oxidant. Alkaline phosphatase (ALP) catalyzes the hydrolysis of ascorbic acid 2-phosphate to release free ascorbic...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2018-04, Vol.185 (4), p.231-9, Article 231 |
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| creator | Xue, Qin Cao, Xuanyu Zhang, Cuiling Xian, Yuezhong |
| description | The authors describe an environmentally friendly and fast (~14 min) method for the synthesis of homogeneously distributed fluorescent polydopamine nanodots (PDA-NDs) using KMnO
4
as the oxidant. Alkaline phosphatase (ALP) catalyzes the hydrolysis of ascorbic acid 2-phosphate to release free ascorbic acid which undergoes an in-situ redox reaction with KMnO
4
. Depending on the activity of ALP, more or less KMnO
4
is consumed, and this affects the formation of the PDA-NDs. Based on this finding, a sensitive method was worked out to quantify the activity of ALP via real-time formation of fluorescent PDA-NDs. The fluorometric signal (best measured at excitation/emission peaks of 390/500 nm) is linear in the 1 to 50 mU·mL
−1
ALP activity range, and the limit of the detection is as low as 0.94 mU·mL
−1
(based on 3 σ/m). The method was successfully applied to the determination of ALP activity in spiked human serum and in MCF-7 cell lysates. It was also applied in a method to screen for inhibitors of ALP.
Graphical abstract
Schematic of a fluorometric method for the determination of alkaline phosphatase (ALP) activity. The method is based on the in-situ regulation of the formation of fluorescent polydopamine nanodots (PDA-NDs) through the competition between the KMnO
4
-induced polymerization of dopamine and ALP-directed ascorbic acid 2-phosphate (Asc-2P) hydrolysis. AA: Ascorbic acid. |
| doi_str_mv | 10.1007/s00604-018-2769-7 |
| format | Article |
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4
as the oxidant. Alkaline phosphatase (ALP) catalyzes the hydrolysis of ascorbic acid 2-phosphate to release free ascorbic acid which undergoes an in-situ redox reaction with KMnO
4
. Depending on the activity of ALP, more or less KMnO
4
is consumed, and this affects the formation of the PDA-NDs. Based on this finding, a sensitive method was worked out to quantify the activity of ALP via real-time formation of fluorescent PDA-NDs. The fluorometric signal (best measured at excitation/emission peaks of 390/500 nm) is linear in the 1 to 50 mU·mL
−1
ALP activity range, and the limit of the detection is as low as 0.94 mU·mL
−1
(based on 3 σ/m). The method was successfully applied to the determination of ALP activity in spiked human serum and in MCF-7 cell lysates. It was also applied in a method to screen for inhibitors of ALP.
Graphical abstract
Schematic of a fluorometric method for the determination of alkaline phosphatase (ALP) activity. The method is based on the in-situ regulation of the formation of fluorescent polydopamine nanodots (PDA-NDs) through the competition between the KMnO
4
-induced polymerization of dopamine and ALP-directed ascorbic acid 2-phosphate (Asc-2P) hydrolysis. AA: Ascorbic acid.</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-018-2769-7</identifier><identifier>PMID: 29594735</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Alkaline phosphatase ; Analytical Chemistry ; Ascorbic acid ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Enzymes ; Fluorescence ; Hydrolysis ; Investigations ; Microengineering ; Nanochemistry ; Nanotechnology ; Organic acids ; Original Paper ; Phosphatase ; Phosphatases ; Phosphates</subject><ispartof>Mikrochimica acta (1966), 2018-04, Vol.185 (4), p.231-9, Article 231</ispartof><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Microchimica Acta is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-b8535a2507047411f8f745936fa453aeb408e954ad912d42c2fbdd2e34ddff003</citedby><cites>FETCH-LOGICAL-c439t-b8535a2507047411f8f745936fa453aeb408e954ad912d42c2fbdd2e34ddff003</cites><orcidid>0000-0001-9535-3658</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-018-2769-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-018-2769-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29594735$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xue, Qin</creatorcontrib><creatorcontrib>Cao, Xuanyu</creatorcontrib><creatorcontrib>Zhang, Cuiling</creatorcontrib><creatorcontrib>Xian, Yuezhong</creatorcontrib><title>Polydopamine nanodots are viable probes for fluorometric determination of the activity of alkaline phosphatase via the in situ regulation of a redox reaction triggered by the enzyme</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><addtitle>Mikrochim Acta</addtitle><description>The authors describe an environmentally friendly and fast (~14 min) method for the synthesis of homogeneously distributed fluorescent polydopamine nanodots (PDA-NDs) using KMnO
4
as the oxidant. Alkaline phosphatase (ALP) catalyzes the hydrolysis of ascorbic acid 2-phosphate to release free ascorbic acid which undergoes an in-situ redox reaction with KMnO
4
. Depending on the activity of ALP, more or less KMnO
4
is consumed, and this affects the formation of the PDA-NDs. Based on this finding, a sensitive method was worked out to quantify the activity of ALP via real-time formation of fluorescent PDA-NDs. The fluorometric signal (best measured at excitation/emission peaks of 390/500 nm) is linear in the 1 to 50 mU·mL
−1
ALP activity range, and the limit of the detection is as low as 0.94 mU·mL
−1
(based on 3 σ/m). The method was successfully applied to the determination of ALP activity in spiked human serum and in MCF-7 cell lysates. It was also applied in a method to screen for inhibitors of ALP.
Graphical abstract
Schematic of a fluorometric method for the determination of alkaline phosphatase (ALP) activity. The method is based on the in-situ regulation of the formation of fluorescent polydopamine nanodots (PDA-NDs) through the competition between the KMnO
4
-induced polymerization of dopamine and ALP-directed ascorbic acid 2-phosphate (Asc-2P) hydrolysis. AA: Ascorbic acid.</description><subject>Alkaline phosphatase</subject><subject>Analytical Chemistry</subject><subject>Ascorbic acid</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Enzymes</subject><subject>Fluorescence</subject><subject>Hydrolysis</subject><subject>Investigations</subject><subject>Microengineering</subject><subject>Nanochemistry</subject><subject>Nanotechnology</subject><subject>Organic acids</subject><subject>Original Paper</subject><subject>Phosphatase</subject><subject>Phosphatases</subject><subject>Phosphates</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1ks1u1DAUhSMEokPhAdggS2y6SbEdO46XVcWfVAkWsLac-HrGJbEH26kI79X3w5kprUCgSI58853j65tTVS8JPicYizcJ4xazGpOupqKVtXhUbQhr2ppj0TyuNhjTtm5aQU-qZyldY0xES9nT6oRKLplo-Ka6_RzGxYS9npwH5LUPJuSEdAR043Q_AtrH0ENCNkRkxznEMEGObkAGMsSi0tkFj4JFeQdID9nduLysez1-0-Pqut-FtN_prNPB9AA6j5LLM4qwncd7C132Jvwo62pUauWk7RZKEfXLQQf-5zLB8-qJ1WOCF3fv0-rru7dfLj_UV5_ef7y8uKoH1shc9x1vuKZlGpgJRojtrGBcNq3VjDcaeoY7kJxpIwk1jA7U9sZQaJgx1mLcnFZnR98yhO8zpKwmlwYYR-0hzElRTGSHZRl5QV__hV6HOfrS3Urxcl5LxQO11SMo523IUQ-rqboQhGPeSSYLdf4PqjwGJjcED9aV-h8CchQMMaQUwap9dJOOiyJYrVFRx6ioEhW1RkWtrby6a3juJzD3it_ZKAA9Aql88uUvPNzo_66_APxFy34</recordid><startdate>20180401</startdate><enddate>20180401</enddate><creator>Xue, Qin</creator><creator>Cao, Xuanyu</creator><creator>Zhang, Cuiling</creator><creator>Xian, Yuezhong</creator><general>Springer Vienna</general><general>Springer</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>M0S</scope><scope>M1P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9535-3658</orcidid></search><sort><creationdate>20180401</creationdate><title>Polydopamine nanodots are viable probes for fluorometric determination of the activity of alkaline phosphatase via the in situ regulation of a redox reaction triggered by the enzyme</title><author>Xue, Qin ; Cao, Xuanyu ; Zhang, Cuiling ; Xian, Yuezhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-b8535a2507047411f8f745936fa453aeb408e954ad912d42c2fbdd2e34ddff003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Alkaline phosphatase</topic><topic>Analytical Chemistry</topic><topic>Ascorbic acid</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Enzymes</topic><topic>Fluorescence</topic><topic>Hydrolysis</topic><topic>Investigations</topic><topic>Microengineering</topic><topic>Nanochemistry</topic><topic>Nanotechnology</topic><topic>Organic acids</topic><topic>Original Paper</topic><topic>Phosphatase</topic><topic>Phosphatases</topic><topic>Phosphates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xue, Qin</creatorcontrib><creatorcontrib>Cao, Xuanyu</creatorcontrib><creatorcontrib>Zhang, Cuiling</creatorcontrib><creatorcontrib>Xian, Yuezhong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xue, Qin</au><au>Cao, Xuanyu</au><au>Zhang, Cuiling</au><au>Xian, Yuezhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polydopamine nanodots are viable probes for fluorometric determination of the activity of alkaline phosphatase via the in situ regulation of a redox reaction triggered by the enzyme</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2018-04-01</date><risdate>2018</risdate><volume>185</volume><issue>4</issue><spage>231</spage><epage>9</epage><pages>231-9</pages><artnum>231</artnum><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>The authors describe an environmentally friendly and fast (~14 min) method for the synthesis of homogeneously distributed fluorescent polydopamine nanodots (PDA-NDs) using KMnO
4
as the oxidant. Alkaline phosphatase (ALP) catalyzes the hydrolysis of ascorbic acid 2-phosphate to release free ascorbic acid which undergoes an in-situ redox reaction with KMnO
4
. Depending on the activity of ALP, more or less KMnO
4
is consumed, and this affects the formation of the PDA-NDs. Based on this finding, a sensitive method was worked out to quantify the activity of ALP via real-time formation of fluorescent PDA-NDs. The fluorometric signal (best measured at excitation/emission peaks of 390/500 nm) is linear in the 1 to 50 mU·mL
−1
ALP activity range, and the limit of the detection is as low as 0.94 mU·mL
−1
(based on 3 σ/m). The method was successfully applied to the determination of ALP activity in spiked human serum and in MCF-7 cell lysates. It was also applied in a method to screen for inhibitors of ALP.
Graphical abstract
Schematic of a fluorometric method for the determination of alkaline phosphatase (ALP) activity. The method is based on the in-situ regulation of the formation of fluorescent polydopamine nanodots (PDA-NDs) through the competition between the KMnO
4
-induced polymerization of dopamine and ALP-directed ascorbic acid 2-phosphate (Asc-2P) hydrolysis. AA: Ascorbic acid.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>29594735</pmid><doi>10.1007/s00604-018-2769-7</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-9535-3658</orcidid></addata></record> |
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| language | eng |
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| source | SpringerLink Journals |
| subjects | Alkaline phosphatase Analytical Chemistry Ascorbic acid Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Enzymes Fluorescence Hydrolysis Investigations Microengineering Nanochemistry Nanotechnology Organic acids Original Paper Phosphatase Phosphatases Phosphates |
| title | Polydopamine nanodots are viable probes for fluorometric determination of the activity of alkaline phosphatase via the in situ regulation of a redox reaction triggered by the enzyme |
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