Improved intracellular PHA determinations with novel spectrophotometric quantification methodologies based on Sudan black dye

The presence of intracellular polyhydroxyalkanoates (PHAs) is usually studied using Sudan black dye solution (SB). In a previous work it was shown that the PHA could be directly quantified using the absorbance of SB fixed by PHA granules in wet cell samples. In the present paper, the optimum SB amou...

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Veröffentlicht in:	Journal of microbiological methods 2018-05, Vol.148, p.1-11
Hauptverfasser:	Porras, Mauricio A., Villar, Marcelo A., Cubitto, María A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Azo Compounds - metabolism Bacillus megaterium - chemistry Chemometric Extractable poly(hydroxyalkanoate)s (PHAs) Naphthalenes - metabolism Polyhydroxyalkanoates - analysis Spectrophotometric methodology Spectrophotometry - methods Staining and Labeling - methods Sudan black stain
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