Construction of polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity for promoting Schwann cells growth
Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine. In the present study, a composite hydrogel named polyacrylamide...
Gespeichert in:
| Veröffentlicht in: | Journal of biomedical materials research. Part A 2018-07, Vol.106 (7), p.1951-1964 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1964 |
|---|---|
| container_issue | 7 |
| container_start_page | 1951 |
| container_title | Journal of biomedical materials research. Part A |
| container_volume | 106 |
| creator | Zhao, Yinxin Wang, Yingjie Niu, Changmei Zhang, Luzhong Li, Guicai Yang, Yumin |
| description | Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine. In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time. A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized. The effects of the composite hydrogel on Schwann cells growth were evaluated and the related molecular mechanism was further penetrated. The results showed that the prepared PAM/GO/Gel/SA composite hydrogels displayed different color appearance as the function of component variations. The surface morphology, components, swelling ratio, mechanical properties, and porosity were all changed with the concentration alteration of each ingredient, while no obvious degradation behavior was observed, indicating a controllable physicochemical property. The culture of cells exhibited that the composite hydrogels could well support the attachment and proliferation of Schwann cells. The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5SYR1 and PGG1SYR0.5 were higher than those of NC. This study may provide important theoretical and experimental basis for the design and development of hydrogel scaffolds for nerve tissue engineering application. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1951–1964, 2018. |
| doi_str_mv | 10.1002/jbm.a.36393 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2018668071</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2047362218</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3973-4ab82ad88a25468e1a62de3e1bafe20b5285945448333a9ded5f8d8b184126e03</originalsourceid><addsrcrecordid>eNp90Utv1DAUBeAIUdFSWLFHltggoczEz7GX7YhXVcQCWFs3yc3EoyQOdsKQ_8CPxtNpu2DBytfSp6MjnSx7RYsVLQq23pf9ClZcccOfZBdUSpYLo-TT4y1MzplR59nzGPcJq0KyZ9k5M3IjlZEX2Z-tH-IU5mpyfiC-IaPvFqjC0kHvalzvAowtDkj877svdjC5YR197eaeQLdzA0xIKt-PPrp0tUsdfGLk4KaWlM5Div7lpoU0PpAx-N6ngB35VrUHGAZSYddFsgv-MLUvsrMGuogv79_L7MeH99-3n_Lbrx8_b69u84qbDc8FlJpBrTUwKZRGCorVyJGW0CArSsm0NEIKoTnnYGqsZaNrXVItKFNY8Mvs7Sk31fk5Y5xs7-KxCAzo52hZQbVSutjQRN_8Q_d-DkNql5TYcMUY1Um9O6kq-BgDNnYMroewWFrY40g2jWTB3o2U9Ov7zLnssX60D6skwE7g4Dpc_pdlb66_XJ1S_wITzqBV</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2047362218</pqid></control><display><type>article</type><title>Construction of polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity for promoting Schwann cells growth</title><source>Access via Wiley Online Library</source><creator>Zhao, Yinxin ; Wang, Yingjie ; Niu, Changmei ; Zhang, Luzhong ; Li, Guicai ; Yang, Yumin</creator><creatorcontrib>Zhao, Yinxin ; Wang, Yingjie ; Niu, Changmei ; Zhang, Luzhong ; Li, Guicai ; Yang, Yumin</creatorcontrib><description>Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine. In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time. A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized. The effects of the composite hydrogel on Schwann cells growth were evaluated and the related molecular mechanism was further penetrated. The results showed that the prepared PAM/GO/Gel/SA composite hydrogels displayed different color appearance as the function of component variations. The surface morphology, components, swelling ratio, mechanical properties, and porosity were all changed with the concentration alteration of each ingredient, while no obvious degradation behavior was observed, indicating a controllable physicochemical property. The culture of cells exhibited that the composite hydrogels could well support the attachment and proliferation of Schwann cells. The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5SYR1 and PGG1SYR0.5 were higher than those of NC. This study may provide important theoretical and experimental basis for the design and development of hydrogel scaffolds for nerve tissue engineering application. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1951–1964, 2018.</description><identifier>ISSN: 1549-3296</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.36393</identifier><identifier>PMID: 29575695</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Alginic acid ; Biocompatibility ; Biological activity ; Cell culture ; Cell proliferation ; composite hydrogel ; Composite materials ; Degradation ; Design engineering ; Drug delivery ; Drug delivery systems ; Free radical polymerization ; Free radicals ; Gelatin ; Gene expression ; Graphene ; Hydrogels ; Mechanical properties ; Molecular chains ; Morphology ; Myelin ; Myelin basic protein ; peripheral nerve regeneration ; Physicochemical properties ; Polymerization ; Polymers ; Porosity ; Proteins ; Regeneration (physiology) ; Regenerative medicine ; Schwann cells ; Sodium ; Sodium alginate ; Sox10 protein ; Surgical implants ; Swelling ratio ; Tissue engineering</subject><ispartof>Journal of biomedical materials research. Part A, 2018-07, Vol.106 (7), p.1951-1964</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3973-4ab82ad88a25468e1a62de3e1bafe20b5285945448333a9ded5f8d8b184126e03</citedby><cites>FETCH-LOGICAL-c3973-4ab82ad88a25468e1a62de3e1bafe20b5285945448333a9ded5f8d8b184126e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbm.a.36393$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbm.a.36393$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29575695$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Yinxin</creatorcontrib><creatorcontrib>Wang, Yingjie</creatorcontrib><creatorcontrib>Niu, Changmei</creatorcontrib><creatorcontrib>Zhang, Luzhong</creatorcontrib><creatorcontrib>Li, Guicai</creatorcontrib><creatorcontrib>Yang, Yumin</creatorcontrib><title>Construction of polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity for promoting Schwann cells growth</title><title>Journal of biomedical materials research. Part A</title><addtitle>J Biomed Mater Res A</addtitle><description>Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine. In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time. A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized. The effects of the composite hydrogel on Schwann cells growth were evaluated and the related molecular mechanism was further penetrated. The results showed that the prepared PAM/GO/Gel/SA composite hydrogels displayed different color appearance as the function of component variations. The surface morphology, components, swelling ratio, mechanical properties, and porosity were all changed with the concentration alteration of each ingredient, while no obvious degradation behavior was observed, indicating a controllable physicochemical property. The culture of cells exhibited that the composite hydrogels could well support the attachment and proliferation of Schwann cells. The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5SYR1 and PGG1SYR0.5 were higher than those of NC. This study may provide important theoretical and experimental basis for the design and development of hydrogel scaffolds for nerve tissue engineering application. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1951–1964, 2018.</description><subject>Alginic acid</subject><subject>Biocompatibility</subject><subject>Biological activity</subject><subject>Cell culture</subject><subject>Cell proliferation</subject><subject>composite hydrogel</subject><subject>Composite materials</subject><subject>Degradation</subject><subject>Design engineering</subject><subject>Drug delivery</subject><subject>Drug delivery systems</subject><subject>Free radical polymerization</subject><subject>Free radicals</subject><subject>Gelatin</subject><subject>Gene expression</subject><subject>Graphene</subject><subject>Hydrogels</subject><subject>Mechanical properties</subject><subject>Molecular chains</subject><subject>Morphology</subject><subject>Myelin</subject><subject>Myelin basic protein</subject><subject>peripheral nerve regeneration</subject><subject>Physicochemical properties</subject><subject>Polymerization</subject><subject>Polymers</subject><subject>Porosity</subject><subject>Proteins</subject><subject>Regeneration (physiology)</subject><subject>Regenerative medicine</subject><subject>Schwann cells</subject><subject>Sodium</subject><subject>Sodium alginate</subject><subject>Sox10 protein</subject><subject>Surgical implants</subject><subject>Swelling ratio</subject><subject>Tissue engineering</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp90Utv1DAUBeAIUdFSWLFHltggoczEz7GX7YhXVcQCWFs3yc3EoyQOdsKQ_8CPxtNpu2DBytfSp6MjnSx7RYsVLQq23pf9ClZcccOfZBdUSpYLo-TT4y1MzplR59nzGPcJq0KyZ9k5M3IjlZEX2Z-tH-IU5mpyfiC-IaPvFqjC0kHvalzvAowtDkj877svdjC5YR197eaeQLdzA0xIKt-PPrp0tUsdfGLk4KaWlM5Div7lpoU0PpAx-N6ngB35VrUHGAZSYddFsgv-MLUvsrMGuogv79_L7MeH99-3n_Lbrx8_b69u84qbDc8FlJpBrTUwKZRGCorVyJGW0CArSsm0NEIKoTnnYGqsZaNrXVItKFNY8Mvs7Sk31fk5Y5xs7-KxCAzo52hZQbVSutjQRN_8Q_d-DkNql5TYcMUY1Um9O6kq-BgDNnYMroewWFrY40g2jWTB3o2U9Ov7zLnssX60D6skwE7g4Dpc_pdlb66_XJ1S_wITzqBV</recordid><startdate>201807</startdate><enddate>201807</enddate><creator>Zhao, Yinxin</creator><creator>Wang, Yingjie</creator><creator>Niu, Changmei</creator><creator>Zhang, Luzhong</creator><creator>Li, Guicai</creator><creator>Yang, Yumin</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201807</creationdate><title>Construction of polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity for promoting Schwann cells growth</title><author>Zhao, Yinxin ; Wang, Yingjie ; Niu, Changmei ; Zhang, Luzhong ; Li, Guicai ; Yang, Yumin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3973-4ab82ad88a25468e1a62de3e1bafe20b5285945448333a9ded5f8d8b184126e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Alginic acid</topic><topic>Biocompatibility</topic><topic>Biological activity</topic><topic>Cell culture</topic><topic>Cell proliferation</topic><topic>composite hydrogel</topic><topic>Composite materials</topic><topic>Degradation</topic><topic>Design engineering</topic><topic>Drug delivery</topic><topic>Drug delivery systems</topic><topic>Free radical polymerization</topic><topic>Free radicals</topic><topic>Gelatin</topic><topic>Gene expression</topic><topic>Graphene</topic><topic>Hydrogels</topic><topic>Mechanical properties</topic><topic>Molecular chains</topic><topic>Morphology</topic><topic>Myelin</topic><topic>Myelin basic protein</topic><topic>peripheral nerve regeneration</topic><topic>Physicochemical properties</topic><topic>Polymerization</topic><topic>Polymers</topic><topic>Porosity</topic><topic>Proteins</topic><topic>Regeneration (physiology)</topic><topic>Regenerative medicine</topic><topic>Schwann cells</topic><topic>Sodium</topic><topic>Sodium alginate</topic><topic>Sox10 protein</topic><topic>Surgical implants</topic><topic>Swelling ratio</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Yinxin</creatorcontrib><creatorcontrib>Wang, Yingjie</creatorcontrib><creatorcontrib>Niu, Changmei</creatorcontrib><creatorcontrib>Zhang, Luzhong</creatorcontrib><creatorcontrib>Li, Guicai</creatorcontrib><creatorcontrib>Yang, Yumin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Yinxin</au><au>Wang, Yingjie</au><au>Niu, Changmei</au><au>Zhang, Luzhong</au><au>Li, Guicai</au><au>Yang, Yumin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity for promoting Schwann cells growth</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J Biomed Mater Res A</addtitle><date>2018-07</date><risdate>2018</risdate><volume>106</volume><issue>7</issue><spage>1951</spage><epage>1964</epage><pages>1951-1964</pages><issn>1549-3296</issn><eissn>1552-4965</eissn><abstract>Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine. In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time. A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized. The effects of the composite hydrogel on Schwann cells growth were evaluated and the related molecular mechanism was further penetrated. The results showed that the prepared PAM/GO/Gel/SA composite hydrogels displayed different color appearance as the function of component variations. The surface morphology, components, swelling ratio, mechanical properties, and porosity were all changed with the concentration alteration of each ingredient, while no obvious degradation behavior was observed, indicating a controllable physicochemical property. The culture of cells exhibited that the composite hydrogels could well support the attachment and proliferation of Schwann cells. The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5SYR1 and PGG1SYR0.5 were higher than those of NC. This study may provide important theoretical and experimental basis for the design and development of hydrogel scaffolds for nerve tissue engineering application. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1951–1964, 2018.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29575695</pmid><doi>10.1002/jbm.a.36393</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1549-3296 |
| ispartof | Journal of biomedical materials research. Part A, 2018-07, Vol.106 (7), p.1951-1964 |
| issn | 1549-3296 1552-4965 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2018668071 |
| source | Access via Wiley Online Library |
| subjects | Alginic acid Biocompatibility Biological activity Cell culture Cell proliferation composite hydrogel Composite materials Degradation Design engineering Drug delivery Drug delivery systems Free radical polymerization Free radicals Gelatin Gene expression Graphene Hydrogels Mechanical properties Molecular chains Morphology Myelin Myelin basic protein peripheral nerve regeneration Physicochemical properties Polymerization Polymers Porosity Proteins Regeneration (physiology) Regenerative medicine Schwann cells Sodium Sodium alginate Sox10 protein Surgical implants Swelling ratio Tissue engineering |
| title | Construction of polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity for promoting Schwann cells growth |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T20%3A29%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Construction%20of%20polyacrylamide/graphene%20oxide/gelatin/sodium%20alginate%20composite%20hydrogel%20with%20bioactivity%20for%20promoting%20Schwann%20cells%20growth&rft.jtitle=Journal%20of%20biomedical%20materials%20research.%20Part%20A&rft.au=Zhao,%20Yinxin&rft.date=2018-07&rft.volume=106&rft.issue=7&rft.spage=1951&rft.epage=1964&rft.pages=1951-1964&rft.issn=1549-3296&rft.eissn=1552-4965&rft_id=info:doi/10.1002/jbm.a.36393&rft_dat=%3Cproquest_cross%3E2047362218%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2047362218&rft_id=info:pmid/29575695&rfr_iscdi=true |