OsERF71 confers drought tolerance via modulating ABA signaling and proline biosynthesis
•The expression of OsERF71 was induced by ABA and various abiotic stresses preferentially in the UR variety, IRAT109.•Overexpression of OsERF71 enhanced the tolerance to drought and high salinity stress in rice.•OsERF71 interference lines were sensitive to drought stress and exhibited a higher rate...
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| Veröffentlicht in: | Plant science (Limerick) 2018-05, Vol.270, p.131-139 |
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| creator | Li, Jinjie Guo, Xiao Zhang, Minghui Wang, Xin Zhao, Yan Yin, Zhigang Zhang, Zhanying Wang, Yanming Xiong, Haiyan Zhang, Hongliang Todorovska, Elena Li, Zichao |
| description | •The expression of OsERF71 was induced by ABA and various abiotic stresses preferentially in the UR variety, IRAT109.•Overexpression of OsERF71 enhanced the tolerance to drought and high salinity stress in rice.•OsERF71 interference lines were sensitive to drought stress and exhibited a higher rate of water loss.•OsERF71 regulated the expression of several ABA- responsive and proline biosynthesis genes under drought stress.•Overexpression of OsERF71 increased sensitivity to exogenous ABA and free proline content, thus playing a positive role in drought stress.
Plants have evolved multiple protective strategies to adapt to adverse environmental conditions. Upland rice (UR) has evolved as a “drought-resistant type”. However, little is known about genes or mechanisms in UR that underlying drought tolerance at the molecular level. Here we report isolation and functional characterization of the ERF gene, OsERF71, from the UR variety, IRAT109. The expression of OsERF71 was induced by abscisic acid (ABA) and various abiotic stresses preferentially in IRAT109 under ABA, dehydration, and polyethyleneglycol (PEG) treatments. OsERF71 was verified as a nuclear-localized protein and had transcriptional activity in yeast cells. Overexpression of the OsERF71 in Nipponbare demonstrated a significant increase in tolerance to drought stress and a reduced rate of water loss. In contrast, OsERF71 interference lines were sensitive to drought stress and exhibited a higher rate of water loss. OsERF71-overexpressing lines also showed enhanced tolerance to high salinity. Moreover, OsERF71 regulated the expression of several ABA- responsive and proline biosynthesis genes under drought stress, resulting in enhanced sensitivity to exogenous ABA treatment and proline accumulation. Accordingly, we suggest that OsERF71 plays a positive role in drought stress tolerance by increasing the expression of genes associated with ABA signaling and proline biosynthesis under stress. |
| doi_str_mv | 10.1016/j.plantsci.2018.01.017 |
| format | Article |
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Plants have evolved multiple protective strategies to adapt to adverse environmental conditions. Upland rice (UR) has evolved as a “drought-resistant type”. However, little is known about genes or mechanisms in UR that underlying drought tolerance at the molecular level. Here we report isolation and functional characterization of the ERF gene, OsERF71, from the UR variety, IRAT109. The expression of OsERF71 was induced by abscisic acid (ABA) and various abiotic stresses preferentially in IRAT109 under ABA, dehydration, and polyethyleneglycol (PEG) treatments. OsERF71 was verified as a nuclear-localized protein and had transcriptional activity in yeast cells. Overexpression of the OsERF71 in Nipponbare demonstrated a significant increase in tolerance to drought stress and a reduced rate of water loss. In contrast, OsERF71 interference lines were sensitive to drought stress and exhibited a higher rate of water loss. OsERF71-overexpressing lines also showed enhanced tolerance to high salinity. Moreover, OsERF71 regulated the expression of several ABA- responsive and proline biosynthesis genes under drought stress, resulting in enhanced sensitivity to exogenous ABA treatment and proline accumulation. Accordingly, we suggest that OsERF71 plays a positive role in drought stress tolerance by increasing the expression of genes associated with ABA signaling and proline biosynthesis under stress.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/j.plantsci.2018.01.017</identifier><identifier>PMID: 29576066</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>ABA ; Abscisic Acid - metabolism ; Drought ; Droughts ; Oryza - genetics ; Oryza - physiology ; Oryza sativa ; OsERF71 ; Plant Growth Regulators - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified ; Proline ; Proline - biosynthesis ; Salinity ; Signal Transduction ; Stress, Physiological ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Upland rice ; Water - metabolism</subject><ispartof>Plant science (Limerick), 2018-05, Vol.270, p.131-139</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-e7349f165e807542ae3b8252e1f1720519dcb3b6cbda8f6937123c38e053a39a3</citedby><cites>FETCH-LOGICAL-c368t-e7349f165e807542ae3b8252e1f1720519dcb3b6cbda8f6937123c38e053a39a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.plantsci.2018.01.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29576066$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Jinjie</creatorcontrib><creatorcontrib>Guo, Xiao</creatorcontrib><creatorcontrib>Zhang, Minghui</creatorcontrib><creatorcontrib>Wang, Xin</creatorcontrib><creatorcontrib>Zhao, Yan</creatorcontrib><creatorcontrib>Yin, Zhigang</creatorcontrib><creatorcontrib>Zhang, Zhanying</creatorcontrib><creatorcontrib>Wang, Yanming</creatorcontrib><creatorcontrib>Xiong, Haiyan</creatorcontrib><creatorcontrib>Zhang, Hongliang</creatorcontrib><creatorcontrib>Todorovska, Elena</creatorcontrib><creatorcontrib>Li, Zichao</creatorcontrib><title>OsERF71 confers drought tolerance via modulating ABA signaling and proline biosynthesis</title><title>Plant science (Limerick)</title><addtitle>Plant Sci</addtitle><description>•The expression of OsERF71 was induced by ABA and various abiotic stresses preferentially in the UR variety, IRAT109.•Overexpression of OsERF71 enhanced the tolerance to drought and high salinity stress in rice.•OsERF71 interference lines were sensitive to drought stress and exhibited a higher rate of water loss.•OsERF71 regulated the expression of several ABA- responsive and proline biosynthesis genes under drought stress.•Overexpression of OsERF71 increased sensitivity to exogenous ABA and free proline content, thus playing a positive role in drought stress.
Plants have evolved multiple protective strategies to adapt to adverse environmental conditions. Upland rice (UR) has evolved as a “drought-resistant type”. However, little is known about genes or mechanisms in UR that underlying drought tolerance at the molecular level. Here we report isolation and functional characterization of the ERF gene, OsERF71, from the UR variety, IRAT109. The expression of OsERF71 was induced by abscisic acid (ABA) and various abiotic stresses preferentially in IRAT109 under ABA, dehydration, and polyethyleneglycol (PEG) treatments. OsERF71 was verified as a nuclear-localized protein and had transcriptional activity in yeast cells. Overexpression of the OsERF71 in Nipponbare demonstrated a significant increase in tolerance to drought stress and a reduced rate of water loss. In contrast, OsERF71 interference lines were sensitive to drought stress and exhibited a higher rate of water loss. OsERF71-overexpressing lines also showed enhanced tolerance to high salinity. Moreover, OsERF71 regulated the expression of several ABA- responsive and proline biosynthesis genes under drought stress, resulting in enhanced sensitivity to exogenous ABA treatment and proline accumulation. Accordingly, we suggest that OsERF71 plays a positive role in drought stress tolerance by increasing the expression of genes associated with ABA signaling and proline biosynthesis under stress.</description><subject>ABA</subject><subject>Abscisic Acid - metabolism</subject><subject>Drought</subject><subject>Droughts</subject><subject>Oryza - genetics</subject><subject>Oryza - physiology</subject><subject>Oryza sativa</subject><subject>OsERF71</subject><subject>Plant Growth Regulators - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified</subject><subject>Proline</subject><subject>Proline - biosynthesis</subject><subject>Salinity</subject><subject>Signal Transduction</subject><subject>Stress, Physiological</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Upland rice</subject><subject>Water - metabolism</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF1LwzAUhoMoOqd_QXLpTWs-lqS9c4pfIAxE8TKk6enMaJuZtAP_vZmb3goHksDznnPyIHRBSU4JlVerfN2afojW5YzQIic0lTpAE1oonjEmykM0SWCRlTPBTtBpjCtCCBNCHaMTVgoliZQT9L6Idy_3imLr-wZCxHXw4_JjwINvIZjeAt44gztfj60ZXL_E85s5jm7Zm3b7Mn2N18GnO-DK-fjVDx8QXTxDR41pI5zvzyl6u797vX3MnhcPT7fz58xyWQwZKD4rGyoFFESJGTPAq4IJBrShihFBy9pWvJK2qk3RyJIryrjlBRDBDS8Nn6LLXd-0xOcIcdCdixbaJAf8GPVWjpSiFCyhcofa4GMM0Oh1cJ0JX5oSvZWqV_pX6k9OE5pKpeDFfsZYdVD_xX4tJuB6B0D66cZB0KkFJHe1C2AHXXv334xvdEaLpA</recordid><startdate>201805</startdate><enddate>201805</enddate><creator>Li, Jinjie</creator><creator>Guo, Xiao</creator><creator>Zhang, Minghui</creator><creator>Wang, Xin</creator><creator>Zhao, Yan</creator><creator>Yin, Zhigang</creator><creator>Zhang, Zhanying</creator><creator>Wang, Yanming</creator><creator>Xiong, Haiyan</creator><creator>Zhang, Hongliang</creator><creator>Todorovska, Elena</creator><creator>Li, Zichao</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201805</creationdate><title>OsERF71 confers drought tolerance via modulating ABA signaling and proline biosynthesis</title><author>Li, Jinjie ; Guo, Xiao ; Zhang, Minghui ; Wang, Xin ; Zhao, Yan ; Yin, Zhigang ; Zhang, Zhanying ; Wang, Yanming ; Xiong, Haiyan ; Zhang, Hongliang ; Todorovska, Elena ; Li, Zichao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-e7349f165e807542ae3b8252e1f1720519dcb3b6cbda8f6937123c38e053a39a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>ABA</topic><topic>Abscisic Acid - metabolism</topic><topic>Drought</topic><topic>Droughts</topic><topic>Oryza - genetics</topic><topic>Oryza - physiology</topic><topic>Oryza sativa</topic><topic>OsERF71</topic><topic>Plant Growth Regulators - metabolism</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants, Genetically Modified</topic><topic>Proline</topic><topic>Proline - biosynthesis</topic><topic>Salinity</topic><topic>Signal Transduction</topic><topic>Stress, Physiological</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Upland rice</topic><topic>Water - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Jinjie</creatorcontrib><creatorcontrib>Guo, Xiao</creatorcontrib><creatorcontrib>Zhang, Minghui</creatorcontrib><creatorcontrib>Wang, Xin</creatorcontrib><creatorcontrib>Zhao, Yan</creatorcontrib><creatorcontrib>Yin, Zhigang</creatorcontrib><creatorcontrib>Zhang, Zhanying</creatorcontrib><creatorcontrib>Wang, Yanming</creatorcontrib><creatorcontrib>Xiong, Haiyan</creatorcontrib><creatorcontrib>Zhang, Hongliang</creatorcontrib><creatorcontrib>Todorovska, Elena</creatorcontrib><creatorcontrib>Li, Zichao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Jinjie</au><au>Guo, Xiao</au><au>Zhang, Minghui</au><au>Wang, Xin</au><au>Zhao, Yan</au><au>Yin, Zhigang</au><au>Zhang, Zhanying</au><au>Wang, Yanming</au><au>Xiong, Haiyan</au><au>Zhang, Hongliang</au><au>Todorovska, Elena</au><au>Li, Zichao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>OsERF71 confers drought tolerance via modulating ABA signaling and proline biosynthesis</atitle><jtitle>Plant science (Limerick)</jtitle><addtitle>Plant Sci</addtitle><date>2018-05</date><risdate>2018</risdate><volume>270</volume><spage>131</spage><epage>139</epage><pages>131-139</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><abstract>•The expression of OsERF71 was induced by ABA and various abiotic stresses preferentially in the UR variety, IRAT109.•Overexpression of OsERF71 enhanced the tolerance to drought and high salinity stress in rice.•OsERF71 interference lines were sensitive to drought stress and exhibited a higher rate of water loss.•OsERF71 regulated the expression of several ABA- responsive and proline biosynthesis genes under drought stress.•Overexpression of OsERF71 increased sensitivity to exogenous ABA and free proline content, thus playing a positive role in drought stress.
Plants have evolved multiple protective strategies to adapt to adverse environmental conditions. Upland rice (UR) has evolved as a “drought-resistant type”. However, little is known about genes or mechanisms in UR that underlying drought tolerance at the molecular level. Here we report isolation and functional characterization of the ERF gene, OsERF71, from the UR variety, IRAT109. The expression of OsERF71 was induced by abscisic acid (ABA) and various abiotic stresses preferentially in IRAT109 under ABA, dehydration, and polyethyleneglycol (PEG) treatments. OsERF71 was verified as a nuclear-localized protein and had transcriptional activity in yeast cells. Overexpression of the OsERF71 in Nipponbare demonstrated a significant increase in tolerance to drought stress and a reduced rate of water loss. In contrast, OsERF71 interference lines were sensitive to drought stress and exhibited a higher rate of water loss. OsERF71-overexpressing lines also showed enhanced tolerance to high salinity. Moreover, OsERF71 regulated the expression of several ABA- responsive and proline biosynthesis genes under drought stress, resulting in enhanced sensitivity to exogenous ABA treatment and proline accumulation. Accordingly, we suggest that OsERF71 plays a positive role in drought stress tolerance by increasing the expression of genes associated with ABA signaling and proline biosynthesis under stress.</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>29576066</pmid><doi>10.1016/j.plantsci.2018.01.017</doi><tpages>9</tpages></addata></record> |
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| ispartof | Plant science (Limerick), 2018-05, Vol.270, p.131-139 |
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| subjects | ABA Abscisic Acid - metabolism Drought Droughts Oryza - genetics Oryza - physiology Oryza sativa OsERF71 Plant Growth Regulators - metabolism Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified Proline Proline - biosynthesis Salinity Signal Transduction Stress, Physiological Transcription Factors - genetics Transcription Factors - metabolism Upland rice Water - metabolism |
| title | OsERF71 confers drought tolerance via modulating ABA signaling and proline biosynthesis |
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