Transplantation of dedifferentiated fat cell-derived micromass pellets contributed to cartilage repair in the rat osteochondral defect model
Mature adipocyte-derived dedifferentiated fat (DFAT) cells possesses the ability to proliferate effectively and the potential to differentiate into multiple linages of mesenchymal tissue; similar to adipose-derived stem cells (ASCs). The purpose of this study is to examine the effects of DFAT cell t...
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| Veröffentlicht in: | Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association 2018-07, Vol.23 (4), p.688-696 |
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| container_title | Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association |
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| creator | Shimizu, Manabu Matsumoto, Taro Kikuta, Shinsuke Ohtaki, Munenori Kano, Koichiro Taniguchi, Hiroaki Saito, Shu Nagaoka, Masahiro Tokuhashi, Yasuaki |
| description | Mature adipocyte-derived dedifferentiated fat (DFAT) cells possesses the ability to proliferate effectively and the potential to differentiate into multiple linages of mesenchymal tissue; similar to adipose-derived stem cells (ASCs). The purpose of this study is to examine the effects of DFAT cell transplantation on cartilage repair in a rat model of osteochondral defects.
Full-thickness osteochondral defects were created in the knees of Sprague–Dawley rats bilaterally. Cartilage-like micromass pellets were prepared from green fluorescent protein (GFP)-labeled rat DFAT cells and subsequently transplanted into the affected right knee of these rats. Defects in the left knee were used as a control. Macroscopic and microscopic changes of treated and control defects were evaluated up to 12 weeks post-treatment with DFAT cells. To observe the transplanted cells, sectioned femurs were immunostained for GFP and type II collagen.
DFAT cells formed micromass pellets expressing characteristics of immature cartilage in vitro. In the DFAT cell-transplanted limbs, the defects were completely filled with white micromass pellets as early as 2 weeks post-treatment. These limbs became smooth at 4 weeks. Conversely, the defects in the control limbs were still not repaired by 4 weeks. Macroscopic ICRS scores at 2 and 4 weeks were significantly higher in the DFAT cells-transplanted limbs compared to those of the control limbs. The modified O'Driscol histological scores for the DFAT cell-transplanted limbs were significantly higher than those of the control limbs at corresponding time points. GFP-positive DAFT cells were detected in the transplanted area at 2 weeks but hardly visible at 12 weeks post-operation.
Transplantation of DFAT cell-derived micromass pellets contribute to cartilage repair in a rat osteochondral defect model. DFAT cell transplantation may be a viable therapeutic strategy for the repair of osteochondral injuries. |
| doi_str_mv | 10.1016/j.jos.2018.03.001 |
| format | Article |
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Full-thickness osteochondral defects were created in the knees of Sprague–Dawley rats bilaterally. Cartilage-like micromass pellets were prepared from green fluorescent protein (GFP)-labeled rat DFAT cells and subsequently transplanted into the affected right knee of these rats. Defects in the left knee were used as a control. Macroscopic and microscopic changes of treated and control defects were evaluated up to 12 weeks post-treatment with DFAT cells. To observe the transplanted cells, sectioned femurs were immunostained for GFP and type II collagen.
DFAT cells formed micromass pellets expressing characteristics of immature cartilage in vitro. In the DFAT cell-transplanted limbs, the defects were completely filled with white micromass pellets as early as 2 weeks post-treatment. These limbs became smooth at 4 weeks. Conversely, the defects in the control limbs were still not repaired by 4 weeks. Macroscopic ICRS scores at 2 and 4 weeks were significantly higher in the DFAT cells-transplanted limbs compared to those of the control limbs. The modified O'Driscol histological scores for the DFAT cell-transplanted limbs were significantly higher than those of the control limbs at corresponding time points. GFP-positive DAFT cells were detected in the transplanted area at 2 weeks but hardly visible at 12 weeks post-operation.
Transplantation of DFAT cell-derived micromass pellets contribute to cartilage repair in a rat osteochondral defect model. DFAT cell transplantation may be a viable therapeutic strategy for the repair of osteochondral injuries.</description><identifier>ISSN: 0949-2658</identifier><identifier>EISSN: 1436-2023</identifier><identifier>DOI: 10.1016/j.jos.2018.03.001</identifier><identifier>PMID: 29571958</identifier><language>eng</language><publisher>Japan: Elsevier B.V</publisher><subject>Adipocytes - transplantation ; Animals ; Cartilage, Articular - injuries ; Cartilage, Articular - pathology ; Cartilage, Articular - surgery ; Cell Differentiation ; Cell Transplantation - methods ; Disease Models, Animal ; Immunohistochemistry ; Knee Joint - pathology ; Knee Joint - surgery ; Male ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Real-Time Polymerase Chain Reaction - methods ; Statistics, Nonparametric ; Treatment Outcome</subject><ispartof>Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association, 2018-07, Vol.23 (4), p.688-696</ispartof><rights>2018 The Authors</rights><rights>Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-b45bc8d0d59fedf99abb0fdcff7dbdc8ec252e2b108fbd6155ece65aac78d0553</citedby><cites>FETCH-LOGICAL-c486t-b45bc8d0d59fedf99abb0fdcff7dbdc8ec252e2b108fbd6155ece65aac78d0553</cites><orcidid>0000-0002-2965-2673</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29571958$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shimizu, Manabu</creatorcontrib><creatorcontrib>Matsumoto, Taro</creatorcontrib><creatorcontrib>Kikuta, Shinsuke</creatorcontrib><creatorcontrib>Ohtaki, Munenori</creatorcontrib><creatorcontrib>Kano, Koichiro</creatorcontrib><creatorcontrib>Taniguchi, Hiroaki</creatorcontrib><creatorcontrib>Saito, Shu</creatorcontrib><creatorcontrib>Nagaoka, Masahiro</creatorcontrib><creatorcontrib>Tokuhashi, Yasuaki</creatorcontrib><title>Transplantation of dedifferentiated fat cell-derived micromass pellets contributed to cartilage repair in the rat osteochondral defect model</title><title>Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association</title><addtitle>J Orthop Sci</addtitle><description>Mature adipocyte-derived dedifferentiated fat (DFAT) cells possesses the ability to proliferate effectively and the potential to differentiate into multiple linages of mesenchymal tissue; similar to adipose-derived stem cells (ASCs). The purpose of this study is to examine the effects of DFAT cell transplantation on cartilage repair in a rat model of osteochondral defects.
Full-thickness osteochondral defects were created in the knees of Sprague–Dawley rats bilaterally. Cartilage-like micromass pellets were prepared from green fluorescent protein (GFP)-labeled rat DFAT cells and subsequently transplanted into the affected right knee of these rats. Defects in the left knee were used as a control. Macroscopic and microscopic changes of treated and control defects were evaluated up to 12 weeks post-treatment with DFAT cells. To observe the transplanted cells, sectioned femurs were immunostained for GFP and type II collagen.
DFAT cells formed micromass pellets expressing characteristics of immature cartilage in vitro. In the DFAT cell-transplanted limbs, the defects were completely filled with white micromass pellets as early as 2 weeks post-treatment. These limbs became smooth at 4 weeks. Conversely, the defects in the control limbs were still not repaired by 4 weeks. Macroscopic ICRS scores at 2 and 4 weeks were significantly higher in the DFAT cells-transplanted limbs compared to those of the control limbs. The modified O'Driscol histological scores for the DFAT cell-transplanted limbs were significantly higher than those of the control limbs at corresponding time points. GFP-positive DAFT cells were detected in the transplanted area at 2 weeks but hardly visible at 12 weeks post-operation.
Transplantation of DFAT cell-derived micromass pellets contribute to cartilage repair in a rat osteochondral defect model. DFAT cell transplantation may be a viable therapeutic strategy for the repair of osteochondral injuries.</description><subject>Adipocytes - transplantation</subject><subject>Animals</subject><subject>Cartilage, Articular - injuries</subject><subject>Cartilage, Articular - pathology</subject><subject>Cartilage, Articular - surgery</subject><subject>Cell Differentiation</subject><subject>Cell Transplantation - methods</subject><subject>Disease Models, Animal</subject><subject>Immunohistochemistry</subject><subject>Knee Joint - pathology</subject><subject>Knee Joint - surgery</subject><subject>Male</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Real-Time Polymerase Chain Reaction - methods</subject><subject>Statistics, Nonparametric</subject><subject>Treatment Outcome</subject><issn>0949-2658</issn><issn>1436-2023</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1uGyEUhVHUKHbcPkA2EctsZgqMmR91FUVpG8lSN-4a8XOpsWZgCthS3iEPXVy7WXaF7tV3DjrnInRHSU0JbT_v631INSO0r0lTE0Kv0JKum7ZihDUf0JIM66FiLe8X6DalfQE6PvAbtGAD7-jA-yV620bp0zxKn2V2weNgsQHjrIUIPjuZwWArM9YwjpWB6I5lMTkdwyRTwnNZQ05YB5-jU4cTngPWMmY3yl-AI8zSRew8zrsyFaeQMgS9C95EOZbPLOiMp2Bg_IiurRwTfLq8K_Tz6_P26Xu1-fHt5elxU-l13-ZKrbnSvSGGDxaMHQapFLFGW9sZZXQPmnEGTFHSW2VayjloaLmUuisqzpsVejj7zjH8PkDKYnLpFFB6CIckTo0S1vUNLSg9oyVwShGsmKObZHwVlIjTEcRelCP8lQjSiNJx0dxf7A9qAvOu-Nd6Ab6cASghjw6iSNqB16X3WMoQJrj_2P8BcqmdSw</recordid><startdate>201807</startdate><enddate>201807</enddate><creator>Shimizu, Manabu</creator><creator>Matsumoto, Taro</creator><creator>Kikuta, Shinsuke</creator><creator>Ohtaki, Munenori</creator><creator>Kano, Koichiro</creator><creator>Taniguchi, Hiroaki</creator><creator>Saito, Shu</creator><creator>Nagaoka, Masahiro</creator><creator>Tokuhashi, Yasuaki</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2965-2673</orcidid></search><sort><creationdate>201807</creationdate><title>Transplantation of dedifferentiated fat cell-derived micromass pellets contributed to cartilage repair in the rat osteochondral defect model</title><author>Shimizu, Manabu ; Matsumoto, Taro ; Kikuta, Shinsuke ; Ohtaki, Munenori ; Kano, Koichiro ; Taniguchi, Hiroaki ; Saito, Shu ; Nagaoka, Masahiro ; Tokuhashi, Yasuaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-b45bc8d0d59fedf99abb0fdcff7dbdc8ec252e2b108fbd6155ece65aac78d0553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adipocytes - transplantation</topic><topic>Animals</topic><topic>Cartilage, Articular - injuries</topic><topic>Cartilage, Articular - pathology</topic><topic>Cartilage, Articular - surgery</topic><topic>Cell Differentiation</topic><topic>Cell Transplantation - methods</topic><topic>Disease Models, Animal</topic><topic>Immunohistochemistry</topic><topic>Knee Joint - pathology</topic><topic>Knee Joint - surgery</topic><topic>Male</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Real-Time Polymerase Chain Reaction - methods</topic><topic>Statistics, Nonparametric</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shimizu, Manabu</creatorcontrib><creatorcontrib>Matsumoto, Taro</creatorcontrib><creatorcontrib>Kikuta, Shinsuke</creatorcontrib><creatorcontrib>Ohtaki, Munenori</creatorcontrib><creatorcontrib>Kano, Koichiro</creatorcontrib><creatorcontrib>Taniguchi, Hiroaki</creatorcontrib><creatorcontrib>Saito, Shu</creatorcontrib><creatorcontrib>Nagaoka, Masahiro</creatorcontrib><creatorcontrib>Tokuhashi, Yasuaki</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shimizu, Manabu</au><au>Matsumoto, Taro</au><au>Kikuta, Shinsuke</au><au>Ohtaki, Munenori</au><au>Kano, Koichiro</au><au>Taniguchi, Hiroaki</au><au>Saito, Shu</au><au>Nagaoka, Masahiro</au><au>Tokuhashi, Yasuaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transplantation of dedifferentiated fat cell-derived micromass pellets contributed to cartilage repair in the rat osteochondral defect model</atitle><jtitle>Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association</jtitle><addtitle>J Orthop Sci</addtitle><date>2018-07</date><risdate>2018</risdate><volume>23</volume><issue>4</issue><spage>688</spage><epage>696</epage><pages>688-696</pages><issn>0949-2658</issn><eissn>1436-2023</eissn><abstract>Mature adipocyte-derived dedifferentiated fat (DFAT) cells possesses the ability to proliferate effectively and the potential to differentiate into multiple linages of mesenchymal tissue; similar to adipose-derived stem cells (ASCs). The purpose of this study is to examine the effects of DFAT cell transplantation on cartilage repair in a rat model of osteochondral defects.
Full-thickness osteochondral defects were created in the knees of Sprague–Dawley rats bilaterally. Cartilage-like micromass pellets were prepared from green fluorescent protein (GFP)-labeled rat DFAT cells and subsequently transplanted into the affected right knee of these rats. Defects in the left knee were used as a control. Macroscopic and microscopic changes of treated and control defects were evaluated up to 12 weeks post-treatment with DFAT cells. To observe the transplanted cells, sectioned femurs were immunostained for GFP and type II collagen.
DFAT cells formed micromass pellets expressing characteristics of immature cartilage in vitro. In the DFAT cell-transplanted limbs, the defects were completely filled with white micromass pellets as early as 2 weeks post-treatment. These limbs became smooth at 4 weeks. Conversely, the defects in the control limbs were still not repaired by 4 weeks. Macroscopic ICRS scores at 2 and 4 weeks were significantly higher in the DFAT cells-transplanted limbs compared to those of the control limbs. The modified O'Driscol histological scores for the DFAT cell-transplanted limbs were significantly higher than those of the control limbs at corresponding time points. GFP-positive DAFT cells were detected in the transplanted area at 2 weeks but hardly visible at 12 weeks post-operation.
Transplantation of DFAT cell-derived micromass pellets contribute to cartilage repair in a rat osteochondral defect model. DFAT cell transplantation may be a viable therapeutic strategy for the repair of osteochondral injuries.</abstract><cop>Japan</cop><pub>Elsevier B.V</pub><pmid>29571958</pmid><doi>10.1016/j.jos.2018.03.001</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-2965-2673</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association, 2018-07, Vol.23 (4), p.688-696 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adipocytes - transplantation Animals Cartilage, Articular - injuries Cartilage, Articular - pathology Cartilage, Articular - surgery Cell Differentiation Cell Transplantation - methods Disease Models, Animal Immunohistochemistry Knee Joint - pathology Knee Joint - surgery Male Random Allocation Rats Rats, Sprague-Dawley Real-Time Polymerase Chain Reaction - methods Statistics, Nonparametric Treatment Outcome |
| title | Transplantation of dedifferentiated fat cell-derived micromass pellets contributed to cartilage repair in the rat osteochondral defect model |
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