Partial mRNA Sequences of the Biomarkers CYP1A, GST, CAT, GR, SOD, GPx, VTG and p53 in Flatfish Syacium gunteri from Gulf of Mexico
The present study reports partial sequences of a group of genes used as exposure and effect biomarkers of organic contaminants and/or heavy metals in Syacium gunteri . In order to isolate these sequences, cDNA was used to amplify fragments between 200 and 800 bp, which were then cloned and sequenced...
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| Veröffentlicht in: | Bulletin of environmental contamination and toxicology 2018-06, Vol.100 (6), p.798-802 |
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| creator | Puch-Hau, Carlos Quintanilla-Mena, Mercedes Rubio-Piña, Jorge Del Río-García, Marcela Zapata-Pérez, Omar |
| description | The present study reports partial sequences of a group of genes used as exposure and effect biomarkers of organic contaminants and/or heavy metals in
Syacium gunteri
. In order to isolate these sequences, cDNA was used to amplify fragments between 200 and 800 bp, which were then cloned and sequenced. The sequences presented high percentages of identity with genes involved in the metabolism of xenobiotic biotransformation (cytochrome P4501A and glutathione S-transferase), oxidative stress (catalase, glutathione reductase, glutathione peroxidase and superoxide dismutase), reproductive system (vitellogenin) and with the tumor suppressor protein p53 reported in the GenBank database. Subsequently, from the deduced sequence of amino acids of each fragment, their tridimensional structure was predicted, using homologous proteins from the Protein Data Base. This study generates an important base of molecular biomarkers for the monitoring of environmental health in the Gulf of Mexico. |
| doi_str_mv | 10.1007/s00128-018-2329-1 |
| format | Article |
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Syacium gunteri
. In order to isolate these sequences, cDNA was used to amplify fragments between 200 and 800 bp, which were then cloned and sequenced. The sequences presented high percentages of identity with genes involved in the metabolism of xenobiotic biotransformation (cytochrome P4501A and glutathione S-transferase), oxidative stress (catalase, glutathione reductase, glutathione peroxidase and superoxide dismutase), reproductive system (vitellogenin) and with the tumor suppressor protein p53 reported in the GenBank database. Subsequently, from the deduced sequence of amino acids of each fragment, their tridimensional structure was predicted, using homologous proteins from the Protein Data Base. This study generates an important base of molecular biomarkers for the monitoring of environmental health in the Gulf of Mexico.</description><identifier>ISSN: 0007-4861</identifier><identifier>EISSN: 1432-0800</identifier><identifier>DOI: 10.1007/s00128-018-2329-1</identifier><identifier>PMID: 29569062</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Amino acids ; Aquatic Pollution ; Biomarkers ; Biotransformation ; Catalase ; Contaminants ; CYP1A protein ; Cytochrome P450 ; Cytochrome P4501A ; Earth and Environmental Science ; Ecotoxicology ; Environment ; Environmental Chemistry ; Environmental Health ; Environmental monitoring ; Gene sequencing ; Genes ; Glutathione ; Glutathione peroxidase ; Glutathione reductase ; Glutathione transferase ; Heavy metals ; Homology ; Metabolism ; mRNA ; Organic contaminants ; Oxidative stress ; p53 Protein ; Peroxidase ; Pollution ; Proteins ; Public health ; Reproductive system ; Soil Science & Conservation ; Superoxide dismutase ; Syacium gunteri ; Tumor suppressor genes ; Vitellogenin ; Waste Water Technology ; Water Management ; Water Pollution Control</subject><ispartof>Bulletin of environmental contamination and toxicology, 2018-06, Vol.100 (6), p.798-802</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>Bulletin of Environmental Contamination and Toxicology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-d7f5fb933db185f77ce4621c4dc94d5a7b74371c839bef294049acb3c89187783</citedby><cites>FETCH-LOGICAL-c372t-d7f5fb933db185f77ce4621c4dc94d5a7b74371c839bef294049acb3c89187783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00128-018-2329-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00128-018-2329-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29569062$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Puch-Hau, Carlos</creatorcontrib><creatorcontrib>Quintanilla-Mena, Mercedes</creatorcontrib><creatorcontrib>Rubio-Piña, Jorge</creatorcontrib><creatorcontrib>Del Río-García, Marcela</creatorcontrib><creatorcontrib>Zapata-Pérez, Omar</creatorcontrib><title>Partial mRNA Sequences of the Biomarkers CYP1A, GST, CAT, GR, SOD, GPx, VTG and p53 in Flatfish Syacium gunteri from Gulf of Mexico</title><title>Bulletin of environmental contamination and toxicology</title><addtitle>Bull Environ Contam Toxicol</addtitle><addtitle>Bull Environ Contam Toxicol</addtitle><description>The present study reports partial sequences of a group of genes used as exposure and effect biomarkers of organic contaminants and/or heavy metals in
Syacium gunteri
. In order to isolate these sequences, cDNA was used to amplify fragments between 200 and 800 bp, which were then cloned and sequenced. The sequences presented high percentages of identity with genes involved in the metabolism of xenobiotic biotransformation (cytochrome P4501A and glutathione S-transferase), oxidative stress (catalase, glutathione reductase, glutathione peroxidase and superoxide dismutase), reproductive system (vitellogenin) and with the tumor suppressor protein p53 reported in the GenBank database. Subsequently, from the deduced sequence of amino acids of each fragment, their tridimensional structure was predicted, using homologous proteins from the Protein Data Base. This study generates an important base of molecular biomarkers for the monitoring of environmental health in the Gulf of Mexico.</description><subject>Amino acids</subject><subject>Aquatic Pollution</subject><subject>Biomarkers</subject><subject>Biotransformation</subject><subject>Catalase</subject><subject>Contaminants</subject><subject>CYP1A protein</subject><subject>Cytochrome P450</subject><subject>Cytochrome P4501A</subject><subject>Earth and Environmental Science</subject><subject>Ecotoxicology</subject><subject>Environment</subject><subject>Environmental Chemistry</subject><subject>Environmental Health</subject><subject>Environmental monitoring</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Glutathione</subject><subject>Glutathione peroxidase</subject><subject>Glutathione reductase</subject><subject>Glutathione transferase</subject><subject>Heavy metals</subject><subject>Homology</subject><subject>Metabolism</subject><subject>mRNA</subject><subject>Organic contaminants</subject><subject>Oxidative stress</subject><subject>p53 Protein</subject><subject>Peroxidase</subject><subject>Pollution</subject><subject>Proteins</subject><subject>Public health</subject><subject>Reproductive system</subject><subject>Soil Science & Conservation</subject><subject>Superoxide dismutase</subject><subject>Syacium gunteri</subject><subject>Tumor suppressor genes</subject><subject>Vitellogenin</subject><subject>Waste Water Technology</subject><subject>Water Management</subject><subject>Water Pollution 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from Gulf of Mexico</title><author>Puch-Hau, Carlos ; Quintanilla-Mena, Mercedes ; Rubio-Piña, Jorge ; Del Río-García, Marcela ; Zapata-Pérez, Omar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-d7f5fb933db185f77ce4621c4dc94d5a7b74371c839bef294049acb3c89187783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Amino acids</topic><topic>Aquatic Pollution</topic><topic>Biomarkers</topic><topic>Biotransformation</topic><topic>Catalase</topic><topic>Contaminants</topic><topic>CYP1A protein</topic><topic>Cytochrome P450</topic><topic>Cytochrome P4501A</topic><topic>Earth and Environmental Science</topic><topic>Ecotoxicology</topic><topic>Environment</topic><topic>Environmental Chemistry</topic><topic>Environmental Health</topic><topic>Environmental monitoring</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Glutathione</topic><topic>Glutathione 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Syacium gunteri
. In order to isolate these sequences, cDNA was used to amplify fragments between 200 and 800 bp, which were then cloned and sequenced. The sequences presented high percentages of identity with genes involved in the metabolism of xenobiotic biotransformation (cytochrome P4501A and glutathione S-transferase), oxidative stress (catalase, glutathione reductase, glutathione peroxidase and superoxide dismutase), reproductive system (vitellogenin) and with the tumor suppressor protein p53 reported in the GenBank database. Subsequently, from the deduced sequence of amino acids of each fragment, their tridimensional structure was predicted, using homologous proteins from the Protein Data Base. This study generates an important base of molecular biomarkers for the monitoring of environmental health in the Gulf of Mexico.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>29569062</pmid><doi>10.1007/s00128-018-2329-1</doi><tpages>5</tpages></addata></record> |
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| subjects | Amino acids Aquatic Pollution Biomarkers Biotransformation Catalase Contaminants CYP1A protein Cytochrome P450 Cytochrome P4501A Earth and Environmental Science Ecotoxicology Environment Environmental Chemistry Environmental Health Environmental monitoring Gene sequencing Genes Glutathione Glutathione peroxidase Glutathione reductase Glutathione transferase Heavy metals Homology Metabolism mRNA Organic contaminants Oxidative stress p53 Protein Peroxidase Pollution Proteins Public health Reproductive system Soil Science & Conservation Superoxide dismutase Syacium gunteri Tumor suppressor genes Vitellogenin Waste Water Technology Water Management Water Pollution Control |
| title | Partial mRNA Sequences of the Biomarkers CYP1A, GST, CAT, GR, SOD, GPx, VTG and p53 in Flatfish Syacium gunteri from Gulf of Mexico |
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