Effect of reduced glutathione supplementation on cryopreservation induced sperm cryoinjuries in Murrah bull semen
•Cryopreservation leads to cryoinjuries in the sperm due to various stresses during cooling and freezing.•Supplementation of an antioxidant, reduced glutathione in Murrah bull semen protect sperm from cryoinjuries.•Study has been undertaken in Murrah buffalo bull, which is an elite class of buffalo...
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| Veröffentlicht in: | Animal reproduction science 2018-05, Vol.192, p.171-178 |
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| creator | Gangwar, Chetna Saxena, Atul Patel, Akhil Singh, S P Yadav, Sarvajeet Kumar, Ravindra Singh, Vijay |
| description | •Cryopreservation leads to cryoinjuries in the sperm due to various stresses during cooling and freezing.•Supplementation of an antioxidant, reduced glutathione in Murrah bull semen protect sperm from cryoinjuries.•Study has been undertaken in Murrah buffalo bull, which is an elite class of buffalo breed in India.
The experiment was conducted to study cryopreservation induced sperm cryoinjuries and the protective effect of reduced Glutathione supplementation in Murrah bull semen. A total of 20 semen ejaculates were split into two parts after initial examination and were extended in glycerolated egg yolk TRIS diluter (Control group) and glycerolated egg yolk TRIS diluter + 0.5 mM reduced Glutathione (Treatment Group). The diluted semen samples were loaded into 0.25 ml French mini straw and sealing of straws were done. Thereafter, semen straws were kept for equilibration for 4 h at 5 °C and semen was frozen using a standard cryopreservation protocol in automatic biological freezer. Post-thaw analysis was performed after 24 h of semen storage in liquid nitrogen. Fresh and post-thaw sperm assessments included sperm motility, viability (SYBR-14/PI assay), mitochondrial function (JC-1/PI assay) and plasma membrane integrity (HOST). Cryopreservation of semen in liquid nitrogen induced a marked reduction in post-thaw sperm motility, viability, mitochondrial function and plasma membrane integrity and increased moribund type of sperm (SYBR-14/PI assay) in control group as compared to reduced glutathione treated group. There were significant (P |
| doi_str_mv | 10.1016/j.anireprosci.2018.03.005 |
| format | Article |
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The experiment was conducted to study cryopreservation induced sperm cryoinjuries and the protective effect of reduced Glutathione supplementation in Murrah bull semen. A total of 20 semen ejaculates were split into two parts after initial examination and were extended in glycerolated egg yolk TRIS diluter (Control group) and glycerolated egg yolk TRIS diluter + 0.5 mM reduced Glutathione (Treatment Group). The diluted semen samples were loaded into 0.25 ml French mini straw and sealing of straws were done. Thereafter, semen straws were kept for equilibration for 4 h at 5 °C and semen was frozen using a standard cryopreservation protocol in automatic biological freezer. Post-thaw analysis was performed after 24 h of semen storage in liquid nitrogen. Fresh and post-thaw sperm assessments included sperm motility, viability (SYBR-14/PI assay), mitochondrial function (JC-1/PI assay) and plasma membrane integrity (HOST). Cryopreservation of semen in liquid nitrogen induced a marked reduction in post-thaw sperm motility, viability, mitochondrial function and plasma membrane integrity and increased moribund type of sperm (SYBR-14/PI assay) in control group as compared to reduced glutathione treated group. There were significant (P < 0.05) cryo injuries in frozen-thawed spermatozoa following cryopreservation in buffalo bull semen. The supplementation of reduced glutathione in treatment group exhibited significantly (P < 0.05) lower cryoinjuries during cryopreservation and semen storage in liquid nitrogen. From the study it was concluded that, spermatozoa from Murrah bulls are susceptible to injuries due to cryopreservation in liquid nitrogen, but these cryo induced damage can be protected significantly (P < 0.05) by the use of reduced Glutathione.</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2018.03.005</identifier><identifier>PMID: 29559193</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Cryoinjuries ; Cryopreservation ; Murrah bull ; Reduced glutathione ; Semen</subject><ispartof>Animal reproduction science, 2018-05, Vol.192, p.171-178</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-7a1384c56dfd2c03e9797e2fba2ea142890ec72d25ec6b4fa51afabf53ab31b73</citedby><cites>FETCH-LOGICAL-c443t-7a1384c56dfd2c03e9797e2fba2ea142890ec72d25ec6b4fa51afabf53ab31b73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.anireprosci.2018.03.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29559193$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gangwar, Chetna</creatorcontrib><creatorcontrib>Saxena, Atul</creatorcontrib><creatorcontrib>Patel, Akhil</creatorcontrib><creatorcontrib>Singh, S P</creatorcontrib><creatorcontrib>Yadav, Sarvajeet</creatorcontrib><creatorcontrib>Kumar, Ravindra</creatorcontrib><creatorcontrib>Singh, Vijay</creatorcontrib><title>Effect of reduced glutathione supplementation on cryopreservation induced sperm cryoinjuries in Murrah bull semen</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>•Cryopreservation leads to cryoinjuries in the sperm due to various stresses during cooling and freezing.•Supplementation of an antioxidant, reduced glutathione in Murrah bull semen protect sperm from cryoinjuries.•Study has been undertaken in Murrah buffalo bull, which is an elite class of buffalo breed in India.
The experiment was conducted to study cryopreservation induced sperm cryoinjuries and the protective effect of reduced Glutathione supplementation in Murrah bull semen. A total of 20 semen ejaculates were split into two parts after initial examination and were extended in glycerolated egg yolk TRIS diluter (Control group) and glycerolated egg yolk TRIS diluter + 0.5 mM reduced Glutathione (Treatment Group). The diluted semen samples were loaded into 0.25 ml French mini straw and sealing of straws were done. Thereafter, semen straws were kept for equilibration for 4 h at 5 °C and semen was frozen using a standard cryopreservation protocol in automatic biological freezer. Post-thaw analysis was performed after 24 h of semen storage in liquid nitrogen. Fresh and post-thaw sperm assessments included sperm motility, viability (SYBR-14/PI assay), mitochondrial function (JC-1/PI assay) and plasma membrane integrity (HOST). Cryopreservation of semen in liquid nitrogen induced a marked reduction in post-thaw sperm motility, viability, mitochondrial function and plasma membrane integrity and increased moribund type of sperm (SYBR-14/PI assay) in control group as compared to reduced glutathione treated group. There were significant (P < 0.05) cryo injuries in frozen-thawed spermatozoa following cryopreservation in buffalo bull semen. The supplementation of reduced glutathione in treatment group exhibited significantly (P < 0.05) lower cryoinjuries during cryopreservation and semen storage in liquid nitrogen. From the study it was concluded that, spermatozoa from Murrah bulls are susceptible to injuries due to cryopreservation in liquid nitrogen, but these cryo induced damage can be protected significantly (P < 0.05) by the use of reduced Glutathione.</description><subject>Cryoinjuries</subject><subject>Cryopreservation</subject><subject>Murrah bull</subject><subject>Reduced glutathione</subject><subject>Semen</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNqNkEtLxDAUhYMozjj6F6Tu3LTm0abtUgZfMOJG1yFNb5wMfU3SDMy_N2NHcSkEAueecy73Q-iG4IRgwu82ieyMhcH2TpmEYlIkmCUYZydoToqcxZQyeormmOVFnDKKZ-jCuQ3GOOe8PEczWmZZSUo2R9sHrUGNUa8jC7VXUEefjR_luDZ9B5Hzw9BAC11QghCFp-y-Hyw4sLtJM92UcwPY9ntsuo23BlwYRa_eWrmOKt80kTs0XaIzLRsHV8d_gT4eH96Xz_Hq7elleb-KVZqyMc4lYUWqMl7rmirMoMzLHKiuJAVJUlqUGFROa5qB4lWqZUaklpXOmKwYqXK2QLdTb8C09eBG0RqnoGlkB713ImDjGeOE8GAtJ6sKRJ0FLQZrWmn3gmBxIC424g_xQ7QQmIlAPGSvj2t81UL9m_xBHAzLyQDh2J0BK0IFdIFYKFSjqHvzjzVfn0qcSw</recordid><startdate>20180501</startdate><enddate>20180501</enddate><creator>Gangwar, Chetna</creator><creator>Saxena, Atul</creator><creator>Patel, Akhil</creator><creator>Singh, S P</creator><creator>Yadav, Sarvajeet</creator><creator>Kumar, Ravindra</creator><creator>Singh, Vijay</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180501</creationdate><title>Effect of reduced glutathione supplementation on cryopreservation induced sperm cryoinjuries in Murrah bull semen</title><author>Gangwar, Chetna ; Saxena, Atul ; Patel, Akhil ; Singh, S P ; Yadav, Sarvajeet ; Kumar, Ravindra ; Singh, Vijay</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-7a1384c56dfd2c03e9797e2fba2ea142890ec72d25ec6b4fa51afabf53ab31b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Cryoinjuries</topic><topic>Cryopreservation</topic><topic>Murrah bull</topic><topic>Reduced glutathione</topic><topic>Semen</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gangwar, Chetna</creatorcontrib><creatorcontrib>Saxena, Atul</creatorcontrib><creatorcontrib>Patel, Akhil</creatorcontrib><creatorcontrib>Singh, S P</creatorcontrib><creatorcontrib>Yadav, Sarvajeet</creatorcontrib><creatorcontrib>Kumar, Ravindra</creatorcontrib><creatorcontrib>Singh, Vijay</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gangwar, Chetna</au><au>Saxena, Atul</au><au>Patel, Akhil</au><au>Singh, S P</au><au>Yadav, Sarvajeet</au><au>Kumar, Ravindra</au><au>Singh, Vijay</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of reduced glutathione supplementation on cryopreservation induced sperm cryoinjuries in Murrah bull semen</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2018-05-01</date><risdate>2018</risdate><volume>192</volume><spage>171</spage><epage>178</epage><pages>171-178</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>•Cryopreservation leads to cryoinjuries in the sperm due to various stresses during cooling and freezing.•Supplementation of an antioxidant, reduced glutathione in Murrah bull semen protect sperm from cryoinjuries.•Study has been undertaken in Murrah buffalo bull, which is an elite class of buffalo breed in India.
The experiment was conducted to study cryopreservation induced sperm cryoinjuries and the protective effect of reduced Glutathione supplementation in Murrah bull semen. A total of 20 semen ejaculates were split into two parts after initial examination and were extended in glycerolated egg yolk TRIS diluter (Control group) and glycerolated egg yolk TRIS diluter + 0.5 mM reduced Glutathione (Treatment Group). The diluted semen samples were loaded into 0.25 ml French mini straw and sealing of straws were done. Thereafter, semen straws were kept for equilibration for 4 h at 5 °C and semen was frozen using a standard cryopreservation protocol in automatic biological freezer. Post-thaw analysis was performed after 24 h of semen storage in liquid nitrogen. Fresh and post-thaw sperm assessments included sperm motility, viability (SYBR-14/PI assay), mitochondrial function (JC-1/PI assay) and plasma membrane integrity (HOST). Cryopreservation of semen in liquid nitrogen induced a marked reduction in post-thaw sperm motility, viability, mitochondrial function and plasma membrane integrity and increased moribund type of sperm (SYBR-14/PI assay) in control group as compared to reduced glutathione treated group. There were significant (P < 0.05) cryo injuries in frozen-thawed spermatozoa following cryopreservation in buffalo bull semen. The supplementation of reduced glutathione in treatment group exhibited significantly (P < 0.05) lower cryoinjuries during cryopreservation and semen storage in liquid nitrogen. From the study it was concluded that, spermatozoa from Murrah bulls are susceptible to injuries due to cryopreservation in liquid nitrogen, but these cryo induced damage can be protected significantly (P < 0.05) by the use of reduced Glutathione.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>29559193</pmid><doi>10.1016/j.anireprosci.2018.03.005</doi><tpages>8</tpages></addata></record> |
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| subjects | Cryoinjuries Cryopreservation Murrah bull Reduced glutathione Semen |
| title | Effect of reduced glutathione supplementation on cryopreservation induced sperm cryoinjuries in Murrah bull semen |
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