Distribution patterns of attached and suspended bacteria in pristine and contaminated shallow aquifers studied with an in situ sediment exposure microcosm
We used specially designed microcosms filled with natural substrate to study microbial colonization in a shallow aquifer. Sterilized sediments were exposed to 3 types of groundwater varying in physical, chemical and biological characteristics: (1) pristine groundwater (site PI 92); (2) groundwater i...
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| Veröffentlicht in: | Aquatic microbial ecology : international journal 2002-06, Vol.28 (2), p.117-129 |
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| creator | GRIEBLER, Christian MINDL, Birgit SLEZAK, Doris GEIGER-KAISER, Margot |
| description | We used specially designed microcosms filled with natural substrate to study microbial colonization in a shallow aquifer. Sterilized sediments were exposed to 3 types of groundwater varying in physical, chemical and biological characteristics: (1) pristine groundwater (site PI 92); (2) groundwater in an observation well at a pristine site (OMV 11); and (3) contaminated groundwater at a landfill site (OMV 5). The number of suspended bacteria was always highest at the landfill site (4.0 plus or minus 4.2 [standard deviation, SD] x 10 super(6) cells cm super(-3)), i.e. on average 16 times higher than in the well water (2.5 plus or minus 3.0 x 10 super(5) cells cm super(-3)) and 96 times higher than in the pristine groundwater (4.1 plus or minus 1.3 x 10 super(4) cells cm super(-3)). Sediments in the microcosms were rapidly colonized and the total number of attached bacteria after 10 mo of exposure was highest at the landfill site (1.8 plus or minus 0.4 x 10 super(8) cells cm super(-3)) followed by the sediment incubated in well water (1.5 plus or minus 0.5 x 10 super(8) cells cm super(-3)) and in pristine groundwater (5.0 plus or minus 1.5 x 10 super(7) cells cm super(-3)). As estimated from image analysis, attached cells from the landfill site were on average characterized by higher cell carbon contents (28 plus or minus 36 fg C cell super(-1)) than at the well water (24 plus or minus 23 fg C cell super(-1)) and the pristine groundwater site (21 plus or minus 23 fg C cell super(-1)). The ratio of attached to suspended bacteria after 10 mo of exposure was highest in the microcosm incubated in pristine groundwater (1657:1) and lowest at the contaminated site (59:1). On the basis of our results we emphasize the importance of attached microbial communities in porous subsurface systems and underline the need for groundwater as well as sediment samples for a serious microbiological characterization of the subsurface. Furthermore, the ratio of attached to suspended bacteria in shallow aquifer systems is suggested to be an indicator of prevailing nutrient concentrations. |
| doi_str_mv | 10.3354/ame028117 |
| format | Article |
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Sterilized sediments were exposed to 3 types of groundwater varying in physical, chemical and biological characteristics: (1) pristine groundwater (site PI 92); (2) groundwater in an observation well at a pristine site (OMV 11); and (3) contaminated groundwater at a landfill site (OMV 5). The number of suspended bacteria was always highest at the landfill site (4.0 plus or minus 4.2 [standard deviation, SD] x 10 super(6) cells cm super(-3)), i.e. on average 16 times higher than in the well water (2.5 plus or minus 3.0 x 10 super(5) cells cm super(-3)) and 96 times higher than in the pristine groundwater (4.1 plus or minus 1.3 x 10 super(4) cells cm super(-3)). Sediments in the microcosms were rapidly colonized and the total number of attached bacteria after 10 mo of exposure was highest at the landfill site (1.8 plus or minus 0.4 x 10 super(8) cells cm super(-3)) followed by the sediment incubated in well water (1.5 plus or minus 0.5 x 10 super(8) cells cm super(-3)) and in pristine groundwater (5.0 plus or minus 1.5 x 10 super(7) cells cm super(-3)). As estimated from image analysis, attached cells from the landfill site were on average characterized by higher cell carbon contents (28 plus or minus 36 fg C cell super(-1)) than at the well water (24 plus or minus 23 fg C cell super(-1)) and the pristine groundwater site (21 plus or minus 23 fg C cell super(-1)). The ratio of attached to suspended bacteria after 10 mo of exposure was highest in the microcosm incubated in pristine groundwater (1657:1) and lowest at the contaminated site (59:1). On the basis of our results we emphasize the importance of attached microbial communities in porous subsurface systems and underline the need for groundwater as well as sediment samples for a serious microbiological characterization of the subsurface. Furthermore, the ratio of attached to suspended bacteria in shallow aquifer systems is suggested to be an indicator of prevailing nutrient concentrations.</description><identifier>ISSN: 0948-3055</identifier><identifier>EISSN: 1616-1564</identifier><identifier>DOI: 10.3354/ame028117</identifier><language>eng</language><publisher>Oldendorf/Luhe: Inter-Research</publisher><subject>Animal, plant and microbial ecology ; Biological and medical sciences ; Freshwater ; Fundamental and applied biological sciences. 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Sterilized sediments were exposed to 3 types of groundwater varying in physical, chemical and biological characteristics: (1) pristine groundwater (site PI 92); (2) groundwater in an observation well at a pristine site (OMV 11); and (3) contaminated groundwater at a landfill site (OMV 5). The number of suspended bacteria was always highest at the landfill site (4.0 plus or minus 4.2 [standard deviation, SD] x 10 super(6) cells cm super(-3)), i.e. on average 16 times higher than in the well water (2.5 plus or minus 3.0 x 10 super(5) cells cm super(-3)) and 96 times higher than in the pristine groundwater (4.1 plus or minus 1.3 x 10 super(4) cells cm super(-3)). Sediments in the microcosms were rapidly colonized and the total number of attached bacteria after 10 mo of exposure was highest at the landfill site (1.8 plus or minus 0.4 x 10 super(8) cells cm super(-3)) followed by the sediment incubated in well water (1.5 plus or minus 0.5 x 10 super(8) cells cm super(-3)) and in pristine groundwater (5.0 plus or minus 1.5 x 10 super(7) cells cm super(-3)). As estimated from image analysis, attached cells from the landfill site were on average characterized by higher cell carbon contents (28 plus or minus 36 fg C cell super(-1)) than at the well water (24 plus or minus 23 fg C cell super(-1)) and the pristine groundwater site (21 plus or minus 23 fg C cell super(-1)). The ratio of attached to suspended bacteria after 10 mo of exposure was highest in the microcosm incubated in pristine groundwater (1657:1) and lowest at the contaminated site (59:1). On the basis of our results we emphasize the importance of attached microbial communities in porous subsurface systems and underline the need for groundwater as well as sediment samples for a serious microbiological characterization of the subsurface. Furthermore, the ratio of attached to suspended bacteria in shallow aquifer systems is suggested to be an indicator of prevailing nutrient concentrations.</description><subject>Animal, plant and microbial ecology</subject><subject>Biological and medical sciences</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Microbial ecology</topic><topic>Various environments (extraatmospheric space, air, water)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GRIEBLER, Christian</creatorcontrib><creatorcontrib>MINDL, Birgit</creatorcontrib><creatorcontrib>SLEZAK, Doris</creatorcontrib><creatorcontrib>GEIGER-KAISER, Margot</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Pollution Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Aquatic microbial ecology : international journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GRIEBLER, Christian</au><au>MINDL, Birgit</au><au>SLEZAK, Doris</au><au>GEIGER-KAISER, Margot</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution patterns of attached and suspended bacteria in pristine and contaminated shallow aquifers studied with an in situ sediment exposure microcosm</atitle><jtitle>Aquatic microbial ecology : international journal</jtitle><date>2002-06-26</date><risdate>2002</risdate><volume>28</volume><issue>2</issue><spage>117</spage><epage>129</epage><pages>117-129</pages><issn>0948-3055</issn><eissn>1616-1564</eissn><abstract>We used specially designed microcosms filled with natural substrate to study microbial colonization in a shallow aquifer. Sterilized sediments were exposed to 3 types of groundwater varying in physical, chemical and biological characteristics: (1) pristine groundwater (site PI 92); (2) groundwater in an observation well at a pristine site (OMV 11); and (3) contaminated groundwater at a landfill site (OMV 5). The number of suspended bacteria was always highest at the landfill site (4.0 plus or minus 4.2 [standard deviation, SD] x 10 super(6) cells cm super(-3)), i.e. on average 16 times higher than in the well water (2.5 plus or minus 3.0 x 10 super(5) cells cm super(-3)) and 96 times higher than in the pristine groundwater (4.1 plus or minus 1.3 x 10 super(4) cells cm super(-3)). Sediments in the microcosms were rapidly colonized and the total number of attached bacteria after 10 mo of exposure was highest at the landfill site (1.8 plus or minus 0.4 x 10 super(8) cells cm super(-3)) followed by the sediment incubated in well water (1.5 plus or minus 0.5 x 10 super(8) cells cm super(-3)) and in pristine groundwater (5.0 plus or minus 1.5 x 10 super(7) cells cm super(-3)). As estimated from image analysis, attached cells from the landfill site were on average characterized by higher cell carbon contents (28 plus or minus 36 fg C cell super(-1)) than at the well water (24 plus or minus 23 fg C cell super(-1)) and the pristine groundwater site (21 plus or minus 23 fg C cell super(-1)). The ratio of attached to suspended bacteria after 10 mo of exposure was highest in the microcosm incubated in pristine groundwater (1657:1) and lowest at the contaminated site (59:1). On the basis of our results we emphasize the importance of attached microbial communities in porous subsurface systems and underline the need for groundwater as well as sediment samples for a serious microbiological characterization of the subsurface. Furthermore, the ratio of attached to suspended bacteria in shallow aquifer systems is suggested to be an indicator of prevailing nutrient concentrations.</abstract><cop>Oldendorf/Luhe</cop><pub>Inter-Research</pub><doi>10.3354/ame028117</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | Inter-Research; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animal, plant and microbial ecology Biological and medical sciences Freshwater Fundamental and applied biological sciences. Psychology Microbial ecology Various environments (extraatmospheric space, air, water) |
| title | Distribution patterns of attached and suspended bacteria in pristine and contaminated shallow aquifers studied with an in situ sediment exposure microcosm |
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