Improved sample dispersion in cryo-EM using “perpetually-hydrated” graphene oxide flakes

For many macromolecular complexes, the inability to uniformly disperse solubilized specimen particles within vitreous ice films precludes their analysis by cryo-electron microscopy (cryo-EM). Here, we introduce a sample preparation process using “perpetually-hydrated” graphene oxide flakes as partic...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Journal of structural biology 2018-10, Vol.204 (1), p.75
Hauptverfasser:	Cheung, Martin, Adaniya, Hidehito, Cassidy, Cathal, Yamashita, Masao, Li, Kun-Lung, Taba, Seita, Shintake, Tsumoru
Format:	Artikel
Sprache:	eng
Schlagworte:	Cryo-electron microscopy Cryoelectron Microscopy - methods Graphite - chemistry Sample preparation Specimen Handling - methods Vitreous ice Vitrification
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!