Improved sample dispersion in cryo-EM using “perpetually-hydrated” graphene oxide flakes

Improved sample dispersion in cryo-EM using “perpetually-hydrated” graphene oxide flakes

For many macromolecular complexes, the inability to uniformly disperse solubilized specimen particles within vitreous ice films precludes their analysis by cryo-electron microscopy (cryo-EM). Here, we introduce a sample preparation process using “perpetually-hydrated” graphene oxide flakes as partic...

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Veröffentlicht in:Journal of structural biology 2018-03
Hauptverfasser: Cheung, Martin, Adaniya, Hidehito, Cassidy, Cathal, Yamashita, Masao, Li, Kun-Lung, Taba, Seita, Shintake, Tsumoru
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Sprache:eng
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Cryo-electron microscopy
Sample preparation
Vitreous ice
Vitrification
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container_title Journal of structural biology
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creator Cheung, Martin
Adaniya, Hidehito
Cassidy, Cathal
Yamashita, Masao
Li, Kun-Lung
Taba, Seita
Shintake, Tsumoru
description For many macromolecular complexes, the inability to uniformly disperse solubilized specimen particles within vitreous ice films precludes their analysis by cryo-electron microscopy (cryo-EM). Here, we introduce a sample preparation process using “perpetually-hydrated” graphene oxide flakes as particle support films, and report vastly improved specimen dispersion. Furthermore, we provide evidence that the presence of graphene oxide flakes in vitreous ice results in a significant reduction in electron beam-induced specimen decomposition. The new method introduced in this study incorporates hydrated graphene oxide flakes into a standard sample preparation regime, without the need for additional tools or devices, making it a cost-effective and easily adoptable alternative to currently available sample preparation approaches.
doi_str_mv 10.1016/j.jsb.2018.03.006
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subjects Cryo-electron microscopy
Sample preparation
Vitreous ice
Vitrification
title Improved sample dispersion in cryo-EM using “perpetually-hydrated” graphene oxide flakes
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