The role of poloxamer 188 for cord blood mononuclear cells into megakaryocytes cultivation and induction in three-dimensional WAVE Bioreactor
To observe the effect of poloxamer 188 (P188) on megakaryocyte cultivation and induction from cord blood mononuclear cells in order to obtain more megakaryocyte progenitor cells (MPC). The cord blood mononuclear cells were isolated and inoculated in cell culture bag or cell culture flask respectivel...
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| Veröffentlicht in: | Zhōnghuá xuèyèxué zázhì 2018-01, Vol.39 (1), p.28 |
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| container_title | Zhōnghuá xuèyèxué zázhì |
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| creator | Chen, L Yue, W Xie, X Y Zhang, X Y Lyu, Y Liu, D Q Xi, J F Qu, M Y Fan, Z Fang, F Pei, X T |
| description | To observe the effect of poloxamer 188 (P188) on megakaryocyte cultivation and induction from cord blood mononuclear cells in order to obtain more megakaryocyte progenitor cells (MPC).
The cord blood mononuclear cells were isolated and inoculated in cell culture bag or cell culture flask respectively. The WIGGENS shaker and cell culture bags were used to mimick WAVE Bioreactor for three-dimensional (3D) cell culture, and the P188 was added to induction medium, The cells were detected for morphology, surface marker, viability, and number on day 14.
In the two-dimensional (2D) culture, CD41(+), CD41(+)/CD61(+), CD61(+) megakaryocytic numbers increased significantly after adding P188 (all |
| doi_str_mv | 10.3760/cma.j.issn.0253-2727.2018.01.006 |
| format | Article |
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The cord blood mononuclear cells were isolated and inoculated in cell culture bag or cell culture flask respectively. The WIGGENS shaker and cell culture bags were used to mimick WAVE Bioreactor for three-dimensional (3D) cell culture, and the P188 was added to induction medium, The cells were detected for morphology, surface marker, viability, and number on day 14.
In the two-dimensional (2D) culture, CD41(+), CD41(+)/CD61(+), CD61(+) megakaryocytic numbers increased significantly after adding P188 (all
<0.01). And in the 3D culture of adding P188, the cell volume became larger and the nuclear shape was irregular, the cytoplasm appeared magenta granules, and the megakaryocyte cells became more mature. By 3D culture, the expression of CD41/CD61 was (36.30±1.27)%
(23.95±1.34)%, hence the differentiation for MPC was significantly</description><identifier>ISSN: 0253-2727</identifier><identifier>DOI: 10.3760/cma.j.issn.0253-2727.2018.01.006</identifier><identifier>PMID: 29551029</identifier><language>chi</language><publisher>China</publisher><subject>Bioreactors ; Cell Differentiation ; Cells, Cultured ; Fetal Blood ; Megakaryocytes ; Poloxamer</subject><ispartof>Zhōnghuá xuèyèxué zázhì, 2018-01, Vol.39 (1), p.28</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29551029$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, L</creatorcontrib><creatorcontrib>Yue, W</creatorcontrib><creatorcontrib>Xie, X Y</creatorcontrib><creatorcontrib>Zhang, X Y</creatorcontrib><creatorcontrib>Lyu, Y</creatorcontrib><creatorcontrib>Liu, D Q</creatorcontrib><creatorcontrib>Xi, J F</creatorcontrib><creatorcontrib>Qu, M Y</creatorcontrib><creatorcontrib>Fan, Z</creatorcontrib><creatorcontrib>Fang, F</creatorcontrib><creatorcontrib>Pei, X T</creatorcontrib><title>The role of poloxamer 188 for cord blood mononuclear cells into megakaryocytes cultivation and induction in three-dimensional WAVE Bioreactor</title><title>Zhōnghuá xuèyèxué zázhì</title><addtitle>Zhonghua Xue Ye Xue Za Zhi</addtitle><description>To observe the effect of poloxamer 188 (P188) on megakaryocyte cultivation and induction from cord blood mononuclear cells in order to obtain more megakaryocyte progenitor cells (MPC).
The cord blood mononuclear cells were isolated and inoculated in cell culture bag or cell culture flask respectively. The WIGGENS shaker and cell culture bags were used to mimick WAVE Bioreactor for three-dimensional (3D) cell culture, and the P188 was added to induction medium, The cells were detected for morphology, surface marker, viability, and number on day 14.
In the two-dimensional (2D) culture, CD41(+), CD41(+)/CD61(+), CD61(+) megakaryocytic numbers increased significantly after adding P188 (all
<0.01). And in the 3D culture of adding P188, the cell volume became larger and the nuclear shape was irregular, the cytoplasm appeared magenta granules, and the megakaryocyte cells became more mature. By 3D culture, the expression of CD41/CD61 was (36.30±1.27)%
(23.95±1.34)%, hence the differentiation for MPC was significantly</description><subject>Bioreactors</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Fetal Blood</subject><subject>Megakaryocytes</subject><subject>Poloxamer</subject><issn>0253-2727</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtOxDAMRbMAAYL5BZQlm5YknaTtEhAvCYnNCJYjN3EhkMZD0iL4CP6Z8lxZvjq2dA9jR1KUVW3EsR2gfCp9zrEUSleFqlVdKiGbUshSCLPF9v7zXbbI2XdCy8o0VSV22K5qtZZCtXvsY_WIPFFATj3fUKA3GDBx2TS8p8QtJce7QOT4QJHiZAPCHGMImfs4Eh_wAZ4hvZN9HzFzO4XRv8LoKXKIbmbcZL83H_n4mBAL5weMeY4g8PuTu3N-6ikh2JHSAdvuIWRc_M59tro4X51dFTe3l9dnJzfFRiozFgZdI7RGY5d9C33raiO7rqqXKExjamFb0MouRetA1Bqkcr0C084GllZCXe2zo5-3m0QvE-ZxPfj81Qki0pTXs0g9X6tGzujhLzp1A7r1Jvlhbrv-M1h9AtepeFw</recordid><startdate>20180114</startdate><enddate>20180114</enddate><creator>Chen, L</creator><creator>Yue, W</creator><creator>Xie, X Y</creator><creator>Zhang, X Y</creator><creator>Lyu, Y</creator><creator>Liu, D Q</creator><creator>Xi, J F</creator><creator>Qu, M Y</creator><creator>Fan, Z</creator><creator>Fang, F</creator><creator>Pei, X T</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20180114</creationdate><title>The role of poloxamer 188 for cord blood mononuclear cells into megakaryocytes cultivation and induction in three-dimensional WAVE Bioreactor</title><author>Chen, L ; Yue, W ; Xie, X Y ; Zhang, X Y ; Lyu, Y ; Liu, D Q ; Xi, J F ; Qu, M Y ; Fan, Z ; Fang, F ; Pei, X T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-6ed8055e6c4f9af9d761bb374e068670c9a52c409da075a12df2a690514c1a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2018</creationdate><topic>Bioreactors</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Fetal Blood</topic><topic>Megakaryocytes</topic><topic>Poloxamer</topic><toplevel>online_resources</toplevel><creatorcontrib>Chen, L</creatorcontrib><creatorcontrib>Yue, W</creatorcontrib><creatorcontrib>Xie, X Y</creatorcontrib><creatorcontrib>Zhang, X Y</creatorcontrib><creatorcontrib>Lyu, Y</creatorcontrib><creatorcontrib>Liu, D Q</creatorcontrib><creatorcontrib>Xi, J F</creatorcontrib><creatorcontrib>Qu, M Y</creatorcontrib><creatorcontrib>Fan, Z</creatorcontrib><creatorcontrib>Fang, F</creatorcontrib><creatorcontrib>Pei, X T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhōnghuá xuèyèxué zázhì</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, L</au><au>Yue, W</au><au>Xie, X Y</au><au>Zhang, X Y</au><au>Lyu, Y</au><au>Liu, D Q</au><au>Xi, J F</au><au>Qu, M Y</au><au>Fan, Z</au><au>Fang, F</au><au>Pei, X T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The role of poloxamer 188 for cord blood mononuclear cells into megakaryocytes cultivation and induction in three-dimensional WAVE Bioreactor</atitle><jtitle>Zhōnghuá xuèyèxué zázhì</jtitle><addtitle>Zhonghua Xue Ye Xue Za Zhi</addtitle><date>2018-01-14</date><risdate>2018</risdate><volume>39</volume><issue>1</issue><spage>28</spage><pages>28-</pages><issn>0253-2727</issn><abstract>To observe the effect of poloxamer 188 (P188) on megakaryocyte cultivation and induction from cord blood mononuclear cells in order to obtain more megakaryocyte progenitor cells (MPC).
The cord blood mononuclear cells were isolated and inoculated in cell culture bag or cell culture flask respectively. The WIGGENS shaker and cell culture bags were used to mimick WAVE Bioreactor for three-dimensional (3D) cell culture, and the P188 was added to induction medium, The cells were detected for morphology, surface marker, viability, and number on day 14.
In the two-dimensional (2D) culture, CD41(+), CD41(+)/CD61(+), CD61(+) megakaryocytic numbers increased significantly after adding P188 (all
<0.01). And in the 3D culture of adding P188, the cell volume became larger and the nuclear shape was irregular, the cytoplasm appeared magenta granules, and the megakaryocyte cells became more mature. By 3D culture, the expression of CD41/CD61 was (36.30±1.27)%
(23.95±1.34)%, hence the differentiation for MPC was significantly</abstract><cop>China</cop><pmid>29551029</pmid><doi>10.3760/cma.j.issn.0253-2727.2018.01.006</doi></addata></record> |
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| identifier | ISSN: 0253-2727 |
| ispartof | Zhōnghuá xuèyèxué zázhì, 2018-01, Vol.39 (1), p.28 |
| issn | 0253-2727 |
| language | chi |
| recordid | cdi_proquest_miscellaneous_2015409281 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Bioreactors Cell Differentiation Cells, Cultured Fetal Blood Megakaryocytes Poloxamer |
| title | The role of poloxamer 188 for cord blood mononuclear cells into megakaryocytes cultivation and induction in three-dimensional WAVE Bioreactor |
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