Development of small molecule biosensors by coupling the recognition of the bacterial allosteric transcription factor with isothermal strand displacement amplification
Here, we demonstrate an easy-to-implement and general biosensing strategy by coupling the small-molecule recognition of the bacterial allosteric transcription factor (aTF) with isothermal strand displacement amplification (SDA) in vitro. Based on this strategy, we developed two biosensors for the de...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2018, Vol.54 (38), p.4774-4777 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Yao, Yongpeng Li, Shanshan Cao, Jiaqian Liu, Weiwei Fan, Keqiang Xiang, Wensheng Yang, Keqian Kong, Deming Wang, Weishan |
| description | Here, we demonstrate an easy-to-implement and general biosensing strategy by coupling the small-molecule recognition of the bacterial allosteric transcription factor (aTF) with isothermal strand displacement amplification (SDA) in vitro. Based on this strategy, we developed two biosensors for the detection of an antiseptic, p-hydroxybenzoic acid, and a disease marker, uric acid, using bacterial aTF HosA and HucR, respectively, highlighting the great potential of this strategy for the development of small-molecule biosensors. |
| doi_str_mv | 10.1039/C8CC01764F |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2018, Vol.54 (38), p.4774-4777 |
| issn | 1359-7345 1364-548X |
| language | eng |
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| source | Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Amplification Bacteria Biosensors Coupling Coupling (molecular) Molecular chains Recognition Strategy Transcription factors Uric acid |
| title | Development of small molecule biosensors by coupling the recognition of the bacterial allosteric transcription factor with isothermal strand displacement amplification |
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