Effects of sertoli cell-conditioned medium on ventral midbrain neural stem cells: A preliminary report
The 796RMB cell line is a multipotent stem cell line isolated from human fetal midbrain tissues, a region from which dopamine neurons of the substantia nigra develop. It would be useful to increase the dopaminergic characteristics of this cell line to enhance its usefulness as a cell therapy for Par...
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| Veröffentlicht in: | Neurotoxicity research 2008-09, Vol.13 (3-4), p.241-246 |
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| container_title | Neurotoxicity research |
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| creator | Shamekh, Rania Saporta, Samuel Cameron, Don F. Willing, Alison E. Sanberg, Cindy D. Johe, Karl Sanberg, P. R. |
| description | The 796RMB cell line is a multipotent stem cell line isolated from human fetal midbrain tissues, a region from which dopamine neurons of the substantia nigra develop. It would be useful to increase the dopaminergic characteristics of this cell line to enhance its usefulness as a cell therapy for Parkinson’s disease utilizing transplantation protocols. Sertoli cells and its conditioned media isolated from the testis have been previously shown to enhance tyrosine hydroxylase expression in ventral mesencephalon neurons both
in vitro
and
in vivo.
Therefore, the present preliminary study investigated the ability of Sertoli cell pre-conditioned medium to enhance differentiation of the 796MB cell line toward the domaminergic phenotype. Results showed that secretory products derived from Sertoli cell conditioned medium increased cell proliferation and enhanced dopaminergic neuronal differentiation of the 796RMB cell line. These findings may lead to alternative therapeutic cell transplantation protocols for the treatment of Parkinson’s disease. |
| doi_str_mv | 10.1007/BF03033507 |
| format | Article |
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in vitro
and
in vivo.
Therefore, the present preliminary study investigated the ability of Sertoli cell pre-conditioned medium to enhance differentiation of the 796MB cell line toward the domaminergic phenotype. Results showed that secretory products derived from Sertoli cell conditioned medium increased cell proliferation and enhanced dopaminergic neuronal differentiation of the 796RMB cell line. These findings may lead to alternative therapeutic cell transplantation protocols for the treatment of Parkinson’s disease.</description><identifier>ISSN: 1029-8428</identifier><identifier>EISSN: 1476-3524</identifier><identifier>DOI: 10.1007/BF03033507</identifier><identifier>PMID: 18522903</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Animals ; Animals, Newborn ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Cell Count - methods ; Cell Differentiation - drug effects ; Cell Proliferation - drug effects ; Cells, Cultured ; Culture Media, Conditioned - pharmacology ; Dopamine - metabolism ; Fetus ; Humans ; Male ; Mesencephalon - cytology ; Microtubule-Associated Proteins - metabolism ; Neurobiology ; Neurochemistry ; Neurology ; Neurons - physiology ; Neurosciences ; Pharmacology/Toxicology ; Rats ; Sertoli Cells - chemistry ; Stem Cells - drug effects ; Tyrosine 3-Monooxygenase - metabolism</subject><ispartof>Neurotoxicity research, 2008-09, Vol.13 (3-4), p.241-246</ispartof><rights>Springer 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-d3a976ff0921d9a28a40e40b17454572e9f8e635fff8d953675f405ff7e3e5d53</citedby><cites>FETCH-LOGICAL-c352t-d3a976ff0921d9a28a40e40b17454572e9f8e635fff8d953675f405ff7e3e5d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/BF03033507$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/BF03033507$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18522903$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shamekh, Rania</creatorcontrib><creatorcontrib>Saporta, Samuel</creatorcontrib><creatorcontrib>Cameron, Don F.</creatorcontrib><creatorcontrib>Willing, Alison E.</creatorcontrib><creatorcontrib>Sanberg, Cindy D.</creatorcontrib><creatorcontrib>Johe, Karl</creatorcontrib><creatorcontrib>Sanberg, P. R.</creatorcontrib><title>Effects of sertoli cell-conditioned medium on ventral midbrain neural stem cells: A preliminary report</title><title>Neurotoxicity research</title><addtitle>neurotox res</addtitle><addtitle>Neurotox Res</addtitle><description>The 796RMB cell line is a multipotent stem cell line isolated from human fetal midbrain tissues, a region from which dopamine neurons of the substantia nigra develop. It would be useful to increase the dopaminergic characteristics of this cell line to enhance its usefulness as a cell therapy for Parkinson’s disease utilizing transplantation protocols. Sertoli cells and its conditioned media isolated from the testis have been previously shown to enhance tyrosine hydroxylase expression in ventral mesencephalon neurons both
in vitro
and
in vivo.
Therefore, the present preliminary study investigated the ability of Sertoli cell pre-conditioned medium to enhance differentiation of the 796MB cell line toward the domaminergic phenotype. Results showed that secretory products derived from Sertoli cell conditioned medium increased cell proliferation and enhanced dopaminergic neuronal differentiation of the 796RMB cell line. These findings may lead to alternative therapeutic cell transplantation protocols for the treatment of Parkinson’s disease.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cell Count - methods</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>Culture Media, Conditioned - pharmacology</subject><subject>Dopamine - metabolism</subject><subject>Fetus</subject><subject>Humans</subject><subject>Male</subject><subject>Mesencephalon - cytology</subject><subject>Microtubule-Associated Proteins - metabolism</subject><subject>Neurobiology</subject><subject>Neurochemistry</subject><subject>Neurology</subject><subject>Neurons - physiology</subject><subject>Neurosciences</subject><subject>Pharmacology/Toxicology</subject><subject>Rats</subject><subject>Sertoli Cells - chemistry</subject><subject>Stem Cells - drug effects</subject><subject>Tyrosine 3-Monooxygenase - metabolism</subject><issn>1029-8428</issn><issn>1476-3524</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkD9PwzAQxS0EolBY-ADIEwMo4L9xwlaqFpAqscAcpckZuUrsYDtIfHtcWqkL093pfvf07iF0Rck9JUQ9PC0JJ5xLoo7QGRUqz7hk4jj1hJVZIVgxQechbAhhVObqFE1oIRkrCT9DeqE1NDFgp3EAH11ncANdlzXOtiYaZ6HFPbRm7LGz-Bts9HWHe9OufW0stjBu5xCh_7sLj3iGBw-d6Y2t_Q_2MDgfL9CJrrsAl_s6RR_Lxfv8JVu9Pb_OZ6usSZZj1vK6VLnWpGS0LWtW1IKAIGuqhBRSMSh1ATmXWuuiLSXPldSCpFEBB9lKPkU3O93Bu68RQqx6E7a-agtuDBUjVOSKlQm83YGNdyF40NXgTZ8MV5RU21SrQ6oJvt6rjuuUxQHdx5iAux0Q0sp-gq82bvQ2ffqf3C87w3_z</recordid><startdate>200809</startdate><enddate>200809</enddate><creator>Shamekh, Rania</creator><creator>Saporta, Samuel</creator><creator>Cameron, Don F.</creator><creator>Willing, Alison E.</creator><creator>Sanberg, Cindy D.</creator><creator>Johe, Karl</creator><creator>Sanberg, P. 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R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of sertoli cell-conditioned medium on ventral midbrain neural stem cells: A preliminary report</atitle><jtitle>Neurotoxicity research</jtitle><stitle>neurotox res</stitle><addtitle>Neurotox Res</addtitle><date>2008-09</date><risdate>2008</risdate><volume>13</volume><issue>3-4</issue><spage>241</spage><epage>246</epage><pages>241-246</pages><issn>1029-8428</issn><eissn>1476-3524</eissn><abstract>The 796RMB cell line is a multipotent stem cell line isolated from human fetal midbrain tissues, a region from which dopamine neurons of the substantia nigra develop. It would be useful to increase the dopaminergic characteristics of this cell line to enhance its usefulness as a cell therapy for Parkinson’s disease utilizing transplantation protocols. Sertoli cells and its conditioned media isolated from the testis have been previously shown to enhance tyrosine hydroxylase expression in ventral mesencephalon neurons both
in vitro
and
in vivo.
Therefore, the present preliminary study investigated the ability of Sertoli cell pre-conditioned medium to enhance differentiation of the 796MB cell line toward the domaminergic phenotype. Results showed that secretory products derived from Sertoli cell conditioned medium increased cell proliferation and enhanced dopaminergic neuronal differentiation of the 796RMB cell line. These findings may lead to alternative therapeutic cell transplantation protocols for the treatment of Parkinson’s disease.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>18522903</pmid><doi>10.1007/BF03033507</doi><tpages>6</tpages></addata></record> |
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| ispartof | Neurotoxicity research, 2008-09, Vol.13 (3-4), p.241-246 |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Animals, Newborn Biomedical and Life Sciences Biomedicine Cell Biology Cell Count - methods Cell Differentiation - drug effects Cell Proliferation - drug effects Cells, Cultured Culture Media, Conditioned - pharmacology Dopamine - metabolism Fetus Humans Male Mesencephalon - cytology Microtubule-Associated Proteins - metabolism Neurobiology Neurochemistry Neurology Neurons - physiology Neurosciences Pharmacology/Toxicology Rats Sertoli Cells - chemistry Stem Cells - drug effects Tyrosine 3-Monooxygenase - metabolism |
| title | Effects of sertoli cell-conditioned medium on ventral midbrain neural stem cells: A preliminary report |
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