Engineered red blood cells for capturing circulating tumor cells with high performance
Filtration of circulating tumor cells (CTCs) in peripheral blood is of proven importance for early cancer diagnosis, treatment monitoring, metastasis diagnosis, and prognostic evaluation. However, currently available strategies for enriching CTCs, such as magnetic activated cell sorting (MACS), face...
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| Veröffentlicht in: | Nanoscale 2018-03, Vol.1 (13), p.614-623 |
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| creator | Zhu, Dao-Ming Wu, Lei Suo, Meng Gao, Song Xie, Wei Zan, Ming-Hui Liu, Ao Chen, Bei Wu, Wen-Tao Ji, Li-Wei Chen, Li-ben Huang, Hui-Ming Guo, Shi-Shang Zhang, Wen-Feng Zhao, Xing-Zhong Sun, Zhi-Jun Liu, Wei |
| description | Filtration of circulating tumor cells (CTCs) in peripheral blood is of proven importance for early cancer diagnosis, treatment monitoring, metastasis diagnosis, and prognostic evaluation. However, currently available strategies for enriching CTCs, such as magnetic activated cell sorting (MACS), face serious problems with purity due to nonspecific interactions between beads and leukocytes in the process of capturing. In the present study, the tumor-targeting molecule folic acid (FA) and magnetic nanoparticles (MNPs) were coated on the surface of red blood cells (RBCs) by hydrophobic interaction and chemical conjugation, respectively. The resulting engineered RBCs rapidly adhered to CTCs and the obtained CTC-RBC conjugates were isolated in a magnetic field. After treatment with RBC lysis buffer and centrifugation, CTCs were released and captured. The duration of the entire process was less than three hours. Cell counting showed that the capture efficiency was above 90% and the purity of the obtained CTCs was higher than 75%. The performance of the proposed method exceeded that of MACS® beads (80% for capture efficiency and 20% for purity) under the same conditions. The obtained CTCs could be successfully re-cultured and proliferated
in vitro
. Our engineered RBCs have provided a novel method for enriching rare cells in the physiological environment.
Filtration of circulating tumor cells (CTCs) in peripheral blood is of proven importance for early cancer diagnosis, treatment monitoring, metastasis diagnosis, and prognostic evaluation. |
| doi_str_mv | 10.1039/c7nr08032h |
| format | Article |
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in vitro
. Our engineered RBCs have provided a novel method for enriching rare cells in the physiological environment.
Filtration of circulating tumor cells (CTCs) in peripheral blood is of proven importance for early cancer diagnosis, treatment monitoring, metastasis diagnosis, and prognostic evaluation.</description><identifier>ISSN: 2040-3364</identifier><identifier>EISSN: 2040-3372</identifier><identifier>DOI: 10.1039/c7nr08032h</identifier><identifier>PMID: 29542756</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Beads ; Blood circulation ; Cell Adhesion ; Cell Line, Tumor ; Cell Separation ; Conjugation ; Diagnosis ; Epithelial Cell Adhesion Molecule ; Erythrocytes ; Erythrocytes - cytology ; Folic acid ; Folic Acid - chemistry ; Humans ; Hydrophobicity ; Leukocytes ; Magnetite Nanoparticles ; Neoplastic Cells, Circulating ; Purity ; Tumors</subject><ispartof>Nanoscale, 2018-03, Vol.1 (13), p.614-623</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-46833658903dfcf2fefadad8542a5a9fc0db5b1f7ef3fcb282affdb387522f63</citedby><cites>FETCH-LOGICAL-c426t-46833658903dfcf2fefadad8542a5a9fc0db5b1f7ef3fcb282affdb387522f63</cites><orcidid>0000-0002-6371-7135</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29542756$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Dao-Ming</creatorcontrib><creatorcontrib>Wu, Lei</creatorcontrib><creatorcontrib>Suo, Meng</creatorcontrib><creatorcontrib>Gao, Song</creatorcontrib><creatorcontrib>Xie, Wei</creatorcontrib><creatorcontrib>Zan, Ming-Hui</creatorcontrib><creatorcontrib>Liu, Ao</creatorcontrib><creatorcontrib>Chen, Bei</creatorcontrib><creatorcontrib>Wu, Wen-Tao</creatorcontrib><creatorcontrib>Ji, Li-Wei</creatorcontrib><creatorcontrib>Chen, Li-ben</creatorcontrib><creatorcontrib>Huang, Hui-Ming</creatorcontrib><creatorcontrib>Guo, Shi-Shang</creatorcontrib><creatorcontrib>Zhang, Wen-Feng</creatorcontrib><creatorcontrib>Zhao, Xing-Zhong</creatorcontrib><creatorcontrib>Sun, Zhi-Jun</creatorcontrib><creatorcontrib>Liu, Wei</creatorcontrib><title>Engineered red blood cells for capturing circulating tumor cells with high performance</title><title>Nanoscale</title><addtitle>Nanoscale</addtitle><description>Filtration of circulating tumor cells (CTCs) in peripheral blood is of proven importance for early cancer diagnosis, treatment monitoring, metastasis diagnosis, and prognostic evaluation. However, currently available strategies for enriching CTCs, such as magnetic activated cell sorting (MACS), face serious problems with purity due to nonspecific interactions between beads and leukocytes in the process of capturing. In the present study, the tumor-targeting molecule folic acid (FA) and magnetic nanoparticles (MNPs) were coated on the surface of red blood cells (RBCs) by hydrophobic interaction and chemical conjugation, respectively. The resulting engineered RBCs rapidly adhered to CTCs and the obtained CTC-RBC conjugates were isolated in a magnetic field. After treatment with RBC lysis buffer and centrifugation, CTCs were released and captured. The duration of the entire process was less than three hours. Cell counting showed that the capture efficiency was above 90% and the purity of the obtained CTCs was higher than 75%. The performance of the proposed method exceeded that of MACS® beads (80% for capture efficiency and 20% for purity) under the same conditions. The obtained CTCs could be successfully re-cultured and proliferated
in vitro
. Our engineered RBCs have provided a novel method for enriching rare cells in the physiological environment.
Filtration of circulating tumor cells (CTCs) in peripheral blood is of proven importance for early cancer diagnosis, treatment monitoring, metastasis diagnosis, and prognostic evaluation.</description><subject>Beads</subject><subject>Blood circulation</subject><subject>Cell Adhesion</subject><subject>Cell Line, Tumor</subject><subject>Cell Separation</subject><subject>Conjugation</subject><subject>Diagnosis</subject><subject>Epithelial Cell Adhesion Molecule</subject><subject>Erythrocytes</subject><subject>Erythrocytes - cytology</subject><subject>Folic acid</subject><subject>Folic Acid - chemistry</subject><subject>Humans</subject><subject>Hydrophobicity</subject><subject>Leukocytes</subject><subject>Magnetite Nanoparticles</subject><subject>Neoplastic Cells, Circulating</subject><subject>Purity</subject><subject>Tumors</subject><issn>2040-3364</issn><issn>2040-3372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1LxDAQxYMo7lq9eFcqXkSopkk_j1JWV1gUZPFa0iSz7dIvkwbxvzfdXVfw4GHIwPvN8OYFoXMf3_mYpvc8bhVOMCXlAZoSHGCP0pgc7vsomKATrdcYRymN6DGakDQMSBxGU_Q-a1dVK6WSwh2rqLtOuFzWtXahUy5n_WBU1a5cXiluajaM_WCaUdtQn9VQumW1Kt1eKjvSsJbLU3QErNbybPc6aPk4W2Zzb_H69Jw9LDwekGjwgiix9sIkxVQABwISmGAise5YyFLgWBRh4UMsgQIvSEIYgChoEoeEQEQddLNd26vuw0g95E2lR1uslZ3ROcF-4NtL7d0Ouv6DrjujWmtupNKQBL514aDbLcVVp7WSkPeqapj6yn2cj2HnWfzytgl7buHL3UpTNFLs0Z90LXCxBZTme_X3t6x-9Z-e9wLoN6iIkDc</recordid><startdate>20180329</startdate><enddate>20180329</enddate><creator>Zhu, Dao-Ming</creator><creator>Wu, Lei</creator><creator>Suo, Meng</creator><creator>Gao, Song</creator><creator>Xie, Wei</creator><creator>Zan, Ming-Hui</creator><creator>Liu, Ao</creator><creator>Chen, Bei</creator><creator>Wu, Wen-Tao</creator><creator>Ji, Li-Wei</creator><creator>Chen, Li-ben</creator><creator>Huang, Hui-Ming</creator><creator>Guo, Shi-Shang</creator><creator>Zhang, Wen-Feng</creator><creator>Zhao, Xing-Zhong</creator><creator>Sun, Zhi-Jun</creator><creator>Liu, Wei</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6371-7135</orcidid></search><sort><creationdate>20180329</creationdate><title>Engineered red blood cells for capturing circulating tumor cells with high performance</title><author>Zhu, Dao-Ming ; Wu, Lei ; Suo, Meng ; Gao, Song ; Xie, Wei ; Zan, Ming-Hui ; Liu, Ao ; Chen, Bei ; Wu, Wen-Tao ; Ji, Li-Wei ; Chen, Li-ben ; Huang, Hui-Ming ; Guo, Shi-Shang ; Zhang, Wen-Feng ; Zhao, Xing-Zhong ; Sun, Zhi-Jun ; Liu, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-46833658903dfcf2fefadad8542a5a9fc0db5b1f7ef3fcb282affdb387522f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Beads</topic><topic>Blood circulation</topic><topic>Cell Adhesion</topic><topic>Cell Line, Tumor</topic><topic>Cell Separation</topic><topic>Conjugation</topic><topic>Diagnosis</topic><topic>Epithelial Cell Adhesion Molecule</topic><topic>Erythrocytes</topic><topic>Erythrocytes - cytology</topic><topic>Folic acid</topic><topic>Folic Acid - chemistry</topic><topic>Humans</topic><topic>Hydrophobicity</topic><topic>Leukocytes</topic><topic>Magnetite Nanoparticles</topic><topic>Neoplastic Cells, Circulating</topic><topic>Purity</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Dao-Ming</creatorcontrib><creatorcontrib>Wu, Lei</creatorcontrib><creatorcontrib>Suo, Meng</creatorcontrib><creatorcontrib>Gao, Song</creatorcontrib><creatorcontrib>Xie, Wei</creatorcontrib><creatorcontrib>Zan, Ming-Hui</creatorcontrib><creatorcontrib>Liu, Ao</creatorcontrib><creatorcontrib>Chen, Bei</creatorcontrib><creatorcontrib>Wu, Wen-Tao</creatorcontrib><creatorcontrib>Ji, Li-Wei</creatorcontrib><creatorcontrib>Chen, Li-ben</creatorcontrib><creatorcontrib>Huang, Hui-Ming</creatorcontrib><creatorcontrib>Guo, Shi-Shang</creatorcontrib><creatorcontrib>Zhang, Wen-Feng</creatorcontrib><creatorcontrib>Zhao, Xing-Zhong</creatorcontrib><creatorcontrib>Sun, Zhi-Jun</creatorcontrib><creatorcontrib>Liu, Wei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Nanoscale</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Dao-Ming</au><au>Wu, Lei</au><au>Suo, Meng</au><au>Gao, Song</au><au>Xie, Wei</au><au>Zan, Ming-Hui</au><au>Liu, Ao</au><au>Chen, Bei</au><au>Wu, Wen-Tao</au><au>Ji, Li-Wei</au><au>Chen, Li-ben</au><au>Huang, Hui-Ming</au><au>Guo, Shi-Shang</au><au>Zhang, Wen-Feng</au><au>Zhao, Xing-Zhong</au><au>Sun, Zhi-Jun</au><au>Liu, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Engineered red blood cells for capturing circulating tumor cells with high performance</atitle><jtitle>Nanoscale</jtitle><addtitle>Nanoscale</addtitle><date>2018-03-29</date><risdate>2018</risdate><volume>1</volume><issue>13</issue><spage>614</spage><epage>623</epage><pages>614-623</pages><issn>2040-3364</issn><eissn>2040-3372</eissn><abstract>Filtration of circulating tumor cells (CTCs) in peripheral blood is of proven importance for early cancer diagnosis, treatment monitoring, metastasis diagnosis, and prognostic evaluation. However, currently available strategies for enriching CTCs, such as magnetic activated cell sorting (MACS), face serious problems with purity due to nonspecific interactions between beads and leukocytes in the process of capturing. In the present study, the tumor-targeting molecule folic acid (FA) and magnetic nanoparticles (MNPs) were coated on the surface of red blood cells (RBCs) by hydrophobic interaction and chemical conjugation, respectively. The resulting engineered RBCs rapidly adhered to CTCs and the obtained CTC-RBC conjugates were isolated in a magnetic field. After treatment with RBC lysis buffer and centrifugation, CTCs were released and captured. The duration of the entire process was less than three hours. Cell counting showed that the capture efficiency was above 90% and the purity of the obtained CTCs was higher than 75%. The performance of the proposed method exceeded that of MACS® beads (80% for capture efficiency and 20% for purity) under the same conditions. The obtained CTCs could be successfully re-cultured and proliferated
in vitro
. Our engineered RBCs have provided a novel method for enriching rare cells in the physiological environment.
Filtration of circulating tumor cells (CTCs) in peripheral blood is of proven importance for early cancer diagnosis, treatment monitoring, metastasis diagnosis, and prognostic evaluation.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>29542756</pmid><doi>10.1039/c7nr08032h</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-6371-7135</orcidid></addata></record> |
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| ispartof | Nanoscale, 2018-03, Vol.1 (13), p.614-623 |
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| subjects | Beads Blood circulation Cell Adhesion Cell Line, Tumor Cell Separation Conjugation Diagnosis Epithelial Cell Adhesion Molecule Erythrocytes Erythrocytes - cytology Folic acid Folic Acid - chemistry Humans Hydrophobicity Leukocytes Magnetite Nanoparticles Neoplastic Cells, Circulating Purity Tumors |
| title | Engineered red blood cells for capturing circulating tumor cells with high performance |
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