Fungal endophytes of turmeric (Curcuma longa L.) and their biocontrol potential against pathogens Pythium aphanidermatum and Rhizoctonia solani
Endophytic fungi have been isolated from the healthy turmeric ( Curcuma longa L.) rhizomes from South India. Thirty-one endophytes were identified based on morphological and ITS–rDNA sequence analysis. The isolated endophytes were screened for antagonistic activity against Pythium aphanidermatum (Ed...
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| Veröffentlicht in: | World journal of microbiology & biotechnology 2018-03, Vol.34 (3), p.49-17, Article 49 |
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| description | Endophytic fungi have been isolated from the healthy turmeric (
Curcuma longa
L.) rhizomes from South India. Thirty-one endophytes were identified based on morphological and ITS–rDNA sequence analysis. The isolated endophytes were screened for antagonistic activity against
Pythium aphanidermatum
(Edson) Fitzp., and
Rhizoctonia solani
Kuhn., causing rhizome rot and leaf blight diseases in turmeric respectively. Results revealed that only six endophytes showed > 70% suppression of test pathogens in antagonistic dual culture assays. The endophyte
T. harzianum
TharDOB-31 showed significant in vitro mycelial growth inhibition of
P. aphanidermatum
(76.0%) and
R. solani
(76.9%) when tested by dual culture method. The SEM studies of interaction zone showed morphological abnormalities like parasitism, shriveling, breakage and lysis of hyphae of the pathogens by endophyte TharDOB-31. Selected endophytic isolates recorded multiple plant growth promoting traits in in vitro studies. The rhizome bacterization followed by soil application of endophyte TharDOB-31 showed lowest Percent Disease Incidence of rhizome rot and leaf blight, 13.8 and 11.6% respectively. The treatment of TharDOB-31 exhibited significant increase in plant height (85 cm) and fresh rhizome yield/plant (425 g) in comparison with untreated control under greenhouse condition. The confocal microscopy validates the colonization of the TharDOB-31 in turmeric rhizomes. The secondary metabolites in ethyl acetate extract of TharDOB-31 were found to contain higher number of antifungal compounds by high resolution liquid chromatograph mass spectrometer analysis. Thereby, endophyte
T. harzianum
isolate can be exploited as a potential biocontrol agent for suppressing rhizome rot and leaf blight diseases in turmeric. |
| doi_str_mv | 10.1007/s11274-018-2431-x |
| format | Article |
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Curcuma longa
L.) rhizomes from South India. Thirty-one endophytes were identified based on morphological and ITS–rDNA sequence analysis. The isolated endophytes were screened for antagonistic activity against
Pythium aphanidermatum
(Edson) Fitzp., and
Rhizoctonia solani
Kuhn., causing rhizome rot and leaf blight diseases in turmeric respectively. Results revealed that only six endophytes showed > 70% suppression of test pathogens in antagonistic dual culture assays. The endophyte
T. harzianum
TharDOB-31 showed significant in vitro mycelial growth inhibition of
P. aphanidermatum
(76.0%) and
R. solani
(76.9%) when tested by dual culture method. The SEM studies of interaction zone showed morphological abnormalities like parasitism, shriveling, breakage and lysis of hyphae of the pathogens by endophyte TharDOB-31. Selected endophytic isolates recorded multiple plant growth promoting traits in in vitro studies. The rhizome bacterization followed by soil application of endophyte TharDOB-31 showed lowest Percent Disease Incidence of rhizome rot and leaf blight, 13.8 and 11.6% respectively. The treatment of TharDOB-31 exhibited significant increase in plant height (85 cm) and fresh rhizome yield/plant (425 g) in comparison with untreated control under greenhouse condition. The confocal microscopy validates the colonization of the TharDOB-31 in turmeric rhizomes. The secondary metabolites in ethyl acetate extract of TharDOB-31 were found to contain higher number of antifungal compounds by high resolution liquid chromatograph mass spectrometer analysis. Thereby, endophyte
T. harzianum
isolate can be exploited as a potential biocontrol agent for suppressing rhizome rot and leaf blight diseases in turmeric.</description><identifier>ISSN: 0959-3993</identifier><identifier>EISSN: 1573-0972</identifier><identifier>DOI: 10.1007/s11274-018-2431-x</identifier><identifier>PMID: 29541936</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Abnormalities ; Acetic acid ; Antibiosis ; Antifungal Agents - pharmacology ; Applied Microbiology ; Aquatic plants ; Biochemistry ; Biological control ; Biological Control Agents ; Biomedical and Life Sciences ; Biotechnology ; Blight ; Breakage ; Colonization ; Confocal microscopy ; Curcuma - microbiology ; Curcuma longa ; DNA, Fungal - genetics ; DNA, Ribosomal - genetics ; Endophytes ; Endophytes - classification ; Endophytes - genetics ; Endophytes - isolation & purification ; Endophytes - physiology ; Environmental Engineering/Biotechnology ; Ethyl acetate ; Fungi ; Fungi - classification ; Fungi - genetics ; Fungi - isolation & purification ; Fungi - metabolism ; Fungicides ; Hydrogen Cyanide - metabolism ; Hyphae ; India ; Indoleacetic Acids - metabolism ; Leaf blight ; Leaves ; Life Sciences ; Lysis ; Metabolites ; Microbial Sensitivity Tests ; Microbiology ; Morphology ; Mycelia ; Original Paper ; Parasitism ; Pathogens ; Plant Development ; Plant Diseases - microbiology ; Plant Diseases - prevention & control ; Plant growth ; Plant Leaves - microbiology ; Pythium - drug effects ; Pythium - growth & development ; Pythium - pathogenicity ; Pythium aphanidermatum ; Rhizoctonia - drug effects ; Rhizoctonia - growth & development ; Rhizoctonia - pathogenicity ; Rhizoctonia solani ; Rhizome - microbiology ; Rhizomes ; Rot ; Secondary Metabolism ; Secondary metabolites ; Soil microorganisms</subject><ispartof>World journal of microbiology & biotechnology, 2018-03, Vol.34 (3), p.49-17, Article 49</ispartof><rights>Springer Science+Business Media B.V., part of Springer Nature 2018</rights><rights>World Journal of Microbiology and Biotechnology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-e5d263ff99b216631dc92b5b569c3a300d501fbca08a536996cdce33d803c8443</citedby><cites>FETCH-LOGICAL-c452t-e5d263ff99b216631dc92b5b569c3a300d501fbca08a536996cdce33d803c8443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11274-018-2431-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11274-018-2431-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29541936$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vinayarani, G.</creatorcontrib><creatorcontrib>Prakash, H. S.</creatorcontrib><title>Fungal endophytes of turmeric (Curcuma longa L.) and their biocontrol potential against pathogens Pythium aphanidermatum and Rhizoctonia solani</title><title>World journal of microbiology & biotechnology</title><addtitle>World J Microbiol Biotechnol</addtitle><addtitle>World J Microbiol Biotechnol</addtitle><description>Endophytic fungi have been isolated from the healthy turmeric (
Curcuma longa
L.) rhizomes from South India. Thirty-one endophytes were identified based on morphological and ITS–rDNA sequence analysis. The isolated endophytes were screened for antagonistic activity against
Pythium aphanidermatum
(Edson) Fitzp., and
Rhizoctonia solani
Kuhn., causing rhizome rot and leaf blight diseases in turmeric respectively. Results revealed that only six endophytes showed > 70% suppression of test pathogens in antagonistic dual culture assays. The endophyte
T. harzianum
TharDOB-31 showed significant in vitro mycelial growth inhibition of
P. aphanidermatum
(76.0%) and
R. solani
(76.9%) when tested by dual culture method. The SEM studies of interaction zone showed morphological abnormalities like parasitism, shriveling, breakage and lysis of hyphae of the pathogens by endophyte TharDOB-31. Selected endophytic isolates recorded multiple plant growth promoting traits in in vitro studies. The rhizome bacterization followed by soil application of endophyte TharDOB-31 showed lowest Percent Disease Incidence of rhizome rot and leaf blight, 13.8 and 11.6% respectively. The treatment of TharDOB-31 exhibited significant increase in plant height (85 cm) and fresh rhizome yield/plant (425 g) in comparison with untreated control under greenhouse condition. The confocal microscopy validates the colonization of the TharDOB-31 in turmeric rhizomes. The secondary metabolites in ethyl acetate extract of TharDOB-31 were found to contain higher number of antifungal compounds by high resolution liquid chromatograph mass spectrometer analysis. Thereby, endophyte
T. harzianum
isolate can be exploited as a potential biocontrol agent for suppressing rhizome rot and leaf blight diseases in turmeric.</description><subject>Abnormalities</subject><subject>Acetic acid</subject><subject>Antibiosis</subject><subject>Antifungal Agents - pharmacology</subject><subject>Applied Microbiology</subject><subject>Aquatic plants</subject><subject>Biochemistry</subject><subject>Biological control</subject><subject>Biological Control Agents</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Blight</subject><subject>Breakage</subject><subject>Colonization</subject><subject>Confocal microscopy</subject><subject>Curcuma - microbiology</subject><subject>Curcuma longa</subject><subject>DNA, Fungal - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>Endophytes</subject><subject>Endophytes - classification</subject><subject>Endophytes - genetics</subject><subject>Endophytes - isolation & purification</subject><subject>Endophytes - physiology</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Ethyl acetate</subject><subject>Fungi</subject><subject>Fungi - classification</subject><subject>Fungi - genetics</subject><subject>Fungi - isolation & purification</subject><subject>Fungi - metabolism</subject><subject>Fungicides</subject><subject>Hydrogen Cyanide - metabolism</subject><subject>Hyphae</subject><subject>India</subject><subject>Indoleacetic Acids - metabolism</subject><subject>Leaf blight</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Lysis</subject><subject>Metabolites</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Morphology</subject><subject>Mycelia</subject><subject>Original Paper</subject><subject>Parasitism</subject><subject>Pathogens</subject><subject>Plant Development</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Diseases - prevention & control</subject><subject>Plant growth</subject><subject>Plant Leaves - microbiology</subject><subject>Pythium - drug effects</subject><subject>Pythium - growth & development</subject><subject>Pythium - pathogenicity</subject><subject>Pythium aphanidermatum</subject><subject>Rhizoctonia - drug effects</subject><subject>Rhizoctonia - growth & development</subject><subject>Rhizoctonia - pathogenicity</subject><subject>Rhizoctonia solani</subject><subject>Rhizome - microbiology</subject><subject>Rhizomes</subject><subject>Rot</subject><subject>Secondary Metabolism</subject><subject>Secondary metabolites</subject><subject>Soil microorganisms</subject><issn>0959-3993</issn><issn>1573-0972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kd1qFDEYhoNU2rV6AZ5IoCf1YGp-JpnNYVlaFRYU0eOQSTI7KTPJND_Q7U14y82yrYLgUQjf8775yAPAe4yuMELdp4Qx6doG4XVDWoqbh1dghVlHGyQ6cgJWSDDRUCHoGXiT0h1CNSXoKTgjgrVYUL4Cv2-L36kJWm_CMu6zTTAMMJc42-g0vNyUqMus4BQqBrdXH6HyBubRugh7F3TwOYYJLiFbn10tUjvlfMpwUXkMO-sT_L7PoyszVMuovDM2ziofrrXnx-geg87BOwVTmOr4LXg9qCnZd8_nOfh1e_Nz86XZfvv8dXO9bXTLSG4sM4TTYRCiJ5hzio0WpGc940JTRREyDOGh1wqtFaNcCK6NtpSaNaJ63bb0HFwee5cY7otNWc4uaTvVHWwoSRKEW9zWTyYVvfgHvQsl-rrdgWIcc4y6SuEjpWNIKdpBLtHNKu4lRvJgSx5tyWpLHmzJh5r58Nxc-tmaP4kXPRUgRyDVkd_Z-Pfp_7c-Afn9ogo</recordid><startdate>20180301</startdate><enddate>20180301</enddate><creator>Vinayarani, G.</creator><creator>Prakash, H. 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S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-e5d263ff99b216631dc92b5b569c3a300d501fbca08a536996cdce33d803c8443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Abnormalities</topic><topic>Acetic acid</topic><topic>Antibiosis</topic><topic>Antifungal Agents - pharmacology</topic><topic>Applied Microbiology</topic><topic>Aquatic plants</topic><topic>Biochemistry</topic><topic>Biological control</topic><topic>Biological Control Agents</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Blight</topic><topic>Breakage</topic><topic>Colonization</topic><topic>Confocal microscopy</topic><topic>Curcuma - microbiology</topic><topic>Curcuma longa</topic><topic>DNA, Fungal - genetics</topic><topic>DNA, Ribosomal - genetics</topic><topic>Endophytes</topic><topic>Endophytes - classification</topic><topic>Endophytes - genetics</topic><topic>Endophytes - isolation & purification</topic><topic>Endophytes - physiology</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Ethyl acetate</topic><topic>Fungi</topic><topic>Fungi - classification</topic><topic>Fungi - genetics</topic><topic>Fungi - isolation & purification</topic><topic>Fungi - metabolism</topic><topic>Fungicides</topic><topic>Hydrogen Cyanide - metabolism</topic><topic>Hyphae</topic><topic>India</topic><topic>Indoleacetic Acids - metabolism</topic><topic>Leaf blight</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Lysis</topic><topic>Metabolites</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Morphology</topic><topic>Mycelia</topic><topic>Original Paper</topic><topic>Parasitism</topic><topic>Pathogens</topic><topic>Plant Development</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Diseases - prevention & control</topic><topic>Plant growth</topic><topic>Plant Leaves - microbiology</topic><topic>Pythium - drug effects</topic><topic>Pythium - growth & development</topic><topic>Pythium - pathogenicity</topic><topic>Pythium aphanidermatum</topic><topic>Rhizoctonia - drug effects</topic><topic>Rhizoctonia - growth & development</topic><topic>Rhizoctonia - pathogenicity</topic><topic>Rhizoctonia solani</topic><topic>Rhizome - microbiology</topic><topic>Rhizomes</topic><topic>Rot</topic><topic>Secondary Metabolism</topic><topic>Secondary metabolites</topic><topic>Soil microorganisms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vinayarani, G.</creatorcontrib><creatorcontrib>Prakash, H. 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S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fungal endophytes of turmeric (Curcuma longa L.) and their biocontrol potential against pathogens Pythium aphanidermatum and Rhizoctonia solani</atitle><jtitle>World journal of microbiology & biotechnology</jtitle><stitle>World J Microbiol Biotechnol</stitle><addtitle>World J Microbiol Biotechnol</addtitle><date>2018-03-01</date><risdate>2018</risdate><volume>34</volume><issue>3</issue><spage>49</spage><epage>17</epage><pages>49-17</pages><artnum>49</artnum><issn>0959-3993</issn><eissn>1573-0972</eissn><abstract>Endophytic fungi have been isolated from the healthy turmeric (
Curcuma longa
L.) rhizomes from South India. Thirty-one endophytes were identified based on morphological and ITS–rDNA sequence analysis. The isolated endophytes were screened for antagonistic activity against
Pythium aphanidermatum
(Edson) Fitzp., and
Rhizoctonia solani
Kuhn., causing rhizome rot and leaf blight diseases in turmeric respectively. Results revealed that only six endophytes showed > 70% suppression of test pathogens in antagonistic dual culture assays. The endophyte
T. harzianum
TharDOB-31 showed significant in vitro mycelial growth inhibition of
P. aphanidermatum
(76.0%) and
R. solani
(76.9%) when tested by dual culture method. The SEM studies of interaction zone showed morphological abnormalities like parasitism, shriveling, breakage and lysis of hyphae of the pathogens by endophyte TharDOB-31. Selected endophytic isolates recorded multiple plant growth promoting traits in in vitro studies. The rhizome bacterization followed by soil application of endophyte TharDOB-31 showed lowest Percent Disease Incidence of rhizome rot and leaf blight, 13.8 and 11.6% respectively. The treatment of TharDOB-31 exhibited significant increase in plant height (85 cm) and fresh rhizome yield/plant (425 g) in comparison with untreated control under greenhouse condition. The confocal microscopy validates the colonization of the TharDOB-31 in turmeric rhizomes. The secondary metabolites in ethyl acetate extract of TharDOB-31 were found to contain higher number of antifungal compounds by high resolution liquid chromatograph mass spectrometer analysis. Thereby, endophyte
T. harzianum
isolate can be exploited as a potential biocontrol agent for suppressing rhizome rot and leaf blight diseases in turmeric.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>29541936</pmid><doi>10.1007/s11274-018-2431-x</doi><tpages>17</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0959-3993 |
| ispartof | World journal of microbiology & biotechnology, 2018-03, Vol.34 (3), p.49-17, Article 49 |
| issn | 0959-3993 1573-0972 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2014140072 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Abnormalities Acetic acid Antibiosis Antifungal Agents - pharmacology Applied Microbiology Aquatic plants Biochemistry Biological control Biological Control Agents Biomedical and Life Sciences Biotechnology Blight Breakage Colonization Confocal microscopy Curcuma - microbiology Curcuma longa DNA, Fungal - genetics DNA, Ribosomal - genetics Endophytes Endophytes - classification Endophytes - genetics Endophytes - isolation & purification Endophytes - physiology Environmental Engineering/Biotechnology Ethyl acetate Fungi Fungi - classification Fungi - genetics Fungi - isolation & purification Fungi - metabolism Fungicides Hydrogen Cyanide - metabolism Hyphae India Indoleacetic Acids - metabolism Leaf blight Leaves Life Sciences Lysis Metabolites Microbial Sensitivity Tests Microbiology Morphology Mycelia Original Paper Parasitism Pathogens Plant Development Plant Diseases - microbiology Plant Diseases - prevention & control Plant growth Plant Leaves - microbiology Pythium - drug effects Pythium - growth & development Pythium - pathogenicity Pythium aphanidermatum Rhizoctonia - drug effects Rhizoctonia - growth & development Rhizoctonia - pathogenicity Rhizoctonia solani Rhizome - microbiology Rhizomes Rot Secondary Metabolism Secondary metabolites Soil microorganisms |
| title | Fungal endophytes of turmeric (Curcuma longa L.) and their biocontrol potential against pathogens Pythium aphanidermatum and Rhizoctonia solani |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T16%3A01%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Fungal%20endophytes%20of%20turmeric%20(Curcuma%20longa%20L.)%20and%20their%20biocontrol%20potential%20against%20pathogens%20Pythium%20aphanidermatum%20and%20Rhizoctonia%20solani&rft.jtitle=World%20journal%20of%20microbiology%20&%20biotechnology&rft.au=Vinayarani,%20G.&rft.date=2018-03-01&rft.volume=34&rft.issue=3&rft.spage=49&rft.epage=17&rft.pages=49-17&rft.artnum=49&rft.issn=0959-3993&rft.eissn=1573-0972&rft_id=info:doi/10.1007/s11274-018-2431-x&rft_dat=%3Cproquest_cross%3E2015616107%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2015616107&rft_id=info:pmid/29541936&rfr_iscdi=true |