In vitro adhesion and anti‐inflammatory properties of native Lactobacillus fermentum and Lactobacillus delbrueckii spp
Aims This study aimed at characterizing the adhesion and immune‐stimulatory properties of native probiotic Lactobacillus fermentum (MCC 2759 and MCC 2760) and Lactobacillus delbrueckii MCC 2775. Methods and Results Adhesion of the strains was assessed in Caco‐2 and HT‐29 cell lines. Expression of ad...
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| Veröffentlicht in: | Journal of applied microbiology 2018-07, Vol.125 (1), p.243-256 |
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| creator | Archer, A.C. Kurrey, N.K. Halami, P.M. |
| description | Aims
This study aimed at characterizing the adhesion and immune‐stimulatory properties of native probiotic Lactobacillus fermentum (MCC 2759 and MCC 2760) and Lactobacillus delbrueckii MCC 2775.
Methods and Results
Adhesion of the strains was assessed in Caco‐2 and HT‐29 cell lines. Expression of adhesion and immune markers were evaluated in Caco‐2 cells by real‐time qPCR. The cultures displayed >80% of adhesion to both cell lines and also induced the expression of mucin‐binding protein (mub) gene in the presence of mucin, bile and pancreatin. Adhesion was mediated by carbohydrate and proteinaceous factors. The cultures stimulated the expression of inflammatory cytokines in Caco‐2 cells. However, pro‐inflammatory genes were down‐regulated upon challenge with lipopolysaccharide and IL‐10 was up‐regulated by the cultures. Cell wall extract of L. fermentum MCC 2760 induced the expression of IL‐6 by 5·47‐fold, whereas crude culture filtrate enhanced the expression of IL‐10 by 14·87‐fold compared to LPS control.
Conclusions
The bacterial cultures exhibited strong adhesion and anti‐inflammatory properties.
Significance and Impact of the Study
This is the first report to reveal the role of adhesion markers of L. fermentum and L. delbrueckii by qPCR. The strain‐specific anti‐inflammatory property of native cultures may be useful to alleviate inflammatory conditions and develop a target‐based probiotic. |
| doi_str_mv | 10.1111/jam.13757 |
| format | Article |
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This study aimed at characterizing the adhesion and immune‐stimulatory properties of native probiotic Lactobacillus fermentum (MCC 2759 and MCC 2760) and Lactobacillus delbrueckii MCC 2775.
Methods and Results
Adhesion of the strains was assessed in Caco‐2 and HT‐29 cell lines. Expression of adhesion and immune markers were evaluated in Caco‐2 cells by real‐time qPCR. The cultures displayed >80% of adhesion to both cell lines and also induced the expression of mucin‐binding protein (mub) gene in the presence of mucin, bile and pancreatin. Adhesion was mediated by carbohydrate and proteinaceous factors. The cultures stimulated the expression of inflammatory cytokines in Caco‐2 cells. However, pro‐inflammatory genes were down‐regulated upon challenge with lipopolysaccharide and IL‐10 was up‐regulated by the cultures. Cell wall extract of L. fermentum MCC 2760 induced the expression of IL‐6 by 5·47‐fold, whereas crude culture filtrate enhanced the expression of IL‐10 by 14·87‐fold compared to LPS control.
Conclusions
The bacterial cultures exhibited strong adhesion and anti‐inflammatory properties.
Significance and Impact of the Study
This is the first report to reveal the role of adhesion markers of L. fermentum and L. delbrueckii by qPCR. The strain‐specific anti‐inflammatory property of native cultures may be useful to alleviate inflammatory conditions and develop a target‐based probiotic.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.13757</identifier><identifier>PMID: 29537703</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adhesion ; adhesion potential ; Adhesion tests ; Adhesive strength ; anti‐inflammatory ; Bile ; Biotechnology ; Caco‐2 ; Carbohydrates ; Cell culture ; Cell lines ; Cell walls ; Cytokines ; Filtrate ; Gene expression ; IL‐10 ; Inflammation ; Lactobacillus ; Lactobacillus delbrueckii ; Lactobacillus fermentum ; lipopolysaccharide ; Lipopolysaccharides ; Markers ; Mucin ; Pancreatin ; Probiotics ; Properties (attributes) ; Proteins ; real‐time qPCR ; Yogurt</subject><ispartof>Journal of applied microbiology, 2018-07, Vol.125 (1), p.243-256</ispartof><rights>2018 The Society for Applied Microbiology</rights><rights>2018 The Society for Applied Microbiology.</rights><rights>Copyright © 2018 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3937-78ec8a89c8b84344e8f6e0b23e7036d01d6c9361cfcd6f5794e2b01b1065a8503</citedby><cites>FETCH-LOGICAL-c3937-78ec8a89c8b84344e8f6e0b23e7036d01d6c9361cfcd6f5794e2b01b1065a8503</cites><orcidid>0000-0002-0293-1891</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.13757$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.13757$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29537703$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Archer, A.C.</creatorcontrib><creatorcontrib>Kurrey, N.K.</creatorcontrib><creatorcontrib>Halami, P.M.</creatorcontrib><title>In vitro adhesion and anti‐inflammatory properties of native Lactobacillus fermentum and Lactobacillus delbrueckii spp</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
This study aimed at characterizing the adhesion and immune‐stimulatory properties of native probiotic Lactobacillus fermentum (MCC 2759 and MCC 2760) and Lactobacillus delbrueckii MCC 2775.
Methods and Results
Adhesion of the strains was assessed in Caco‐2 and HT‐29 cell lines. Expression of adhesion and immune markers were evaluated in Caco‐2 cells by real‐time qPCR. The cultures displayed >80% of adhesion to both cell lines and also induced the expression of mucin‐binding protein (mub) gene in the presence of mucin, bile and pancreatin. Adhesion was mediated by carbohydrate and proteinaceous factors. The cultures stimulated the expression of inflammatory cytokines in Caco‐2 cells. However, pro‐inflammatory genes were down‐regulated upon challenge with lipopolysaccharide and IL‐10 was up‐regulated by the cultures. Cell wall extract of L. fermentum MCC 2760 induced the expression of IL‐6 by 5·47‐fold, whereas crude culture filtrate enhanced the expression of IL‐10 by 14·87‐fold compared to LPS control.
Conclusions
The bacterial cultures exhibited strong adhesion and anti‐inflammatory properties.
Significance and Impact of the Study
This is the first report to reveal the role of adhesion markers of L. fermentum and L. delbrueckii by qPCR. The strain‐specific anti‐inflammatory property of native cultures may be useful to alleviate inflammatory conditions and develop a target‐based probiotic.</description><subject>Adhesion</subject><subject>adhesion potential</subject><subject>Adhesion tests</subject><subject>Adhesive strength</subject><subject>anti‐inflammatory</subject><subject>Bile</subject><subject>Biotechnology</subject><subject>Caco‐2</subject><subject>Carbohydrates</subject><subject>Cell culture</subject><subject>Cell lines</subject><subject>Cell walls</subject><subject>Cytokines</subject><subject>Filtrate</subject><subject>Gene expression</subject><subject>IL‐10</subject><subject>Inflammation</subject><subject>Lactobacillus</subject><subject>Lactobacillus delbrueckii</subject><subject>Lactobacillus fermentum</subject><subject>lipopolysaccharide</subject><subject>Lipopolysaccharides</subject><subject>Markers</subject><subject>Mucin</subject><subject>Pancreatin</subject><subject>Probiotics</subject><subject>Properties (attributes)</subject><subject>Proteins</subject><subject>real‐time qPCR</subject><subject>Yogurt</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kctKxDAUhoMo3he-gATc6KKaNM2lSxGvjLjRdUnTU8zYNjVp1dn5CD6jT2KcUUHBQMiBfHz8nB-hHUoOaTxHU90eUia5XELrlAmepEKmy_M5SziR6RraCGFKCGWEi1W0luacSUnYOnq57PCTHbzDurqHYF2HdVfFO9j31zfb1Y1uWz04P8O9dz34wULArsadHuwT4Ik2gyu1sU0zBlyDb6EbxnYu-f1XQVP6EcyDtTj0_RZaqXUTYPvr3UR3Z6e3JxfJ5Ob88uR4khiWM5lIBUZplRtVqoxlGahaAClTBjG-qAithMmZoKY2lai5zDNIS0JLSgTXihO2ifYX3hj_cYQwFK0NBppGd-DGUKRxKVIpktGI7v1Bp270XUwXKc6pVJxlkTpYUMa7EDzURe9tq_2soKT4rKOIdRTzOiK7-2UcyxaqH_J7_xE4WgDPtoHZ_6bi6vh6ofwAMTCWFw</recordid><startdate>201807</startdate><enddate>201807</enddate><creator>Archer, A.C.</creator><creator>Kurrey, N.K.</creator><creator>Halami, P.M.</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0293-1891</orcidid></search><sort><creationdate>201807</creationdate><title>In vitro adhesion and anti‐inflammatory properties of native Lactobacillus fermentum and Lactobacillus delbrueckii spp</title><author>Archer, A.C. ; Kurrey, N.K. ; Halami, P.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3937-78ec8a89c8b84344e8f6e0b23e7036d01d6c9361cfcd6f5794e2b01b1065a8503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adhesion</topic><topic>adhesion potential</topic><topic>Adhesion tests</topic><topic>Adhesive strength</topic><topic>anti‐inflammatory</topic><topic>Bile</topic><topic>Biotechnology</topic><topic>Caco‐2</topic><topic>Carbohydrates</topic><topic>Cell culture</topic><topic>Cell lines</topic><topic>Cell walls</topic><topic>Cytokines</topic><topic>Filtrate</topic><topic>Gene expression</topic><topic>IL‐10</topic><topic>Inflammation</topic><topic>Lactobacillus</topic><topic>Lactobacillus delbrueckii</topic><topic>Lactobacillus fermentum</topic><topic>lipopolysaccharide</topic><topic>Lipopolysaccharides</topic><topic>Markers</topic><topic>Mucin</topic><topic>Pancreatin</topic><topic>Probiotics</topic><topic>Properties (attributes)</topic><topic>Proteins</topic><topic>real‐time qPCR</topic><topic>Yogurt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Archer, A.C.</creatorcontrib><creatorcontrib>Kurrey, N.K.</creatorcontrib><creatorcontrib>Halami, P.M.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Archer, A.C.</au><au>Kurrey, N.K.</au><au>Halami, P.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro adhesion and anti‐inflammatory properties of native Lactobacillus fermentum and Lactobacillus delbrueckii spp</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2018-07</date><risdate>2018</risdate><volume>125</volume><issue>1</issue><spage>243</spage><epage>256</epage><pages>243-256</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
This study aimed at characterizing the adhesion and immune‐stimulatory properties of native probiotic Lactobacillus fermentum (MCC 2759 and MCC 2760) and Lactobacillus delbrueckii MCC 2775.
Methods and Results
Adhesion of the strains was assessed in Caco‐2 and HT‐29 cell lines. Expression of adhesion and immune markers were evaluated in Caco‐2 cells by real‐time qPCR. The cultures displayed >80% of adhesion to both cell lines and also induced the expression of mucin‐binding protein (mub) gene in the presence of mucin, bile and pancreatin. Adhesion was mediated by carbohydrate and proteinaceous factors. The cultures stimulated the expression of inflammatory cytokines in Caco‐2 cells. However, pro‐inflammatory genes were down‐regulated upon challenge with lipopolysaccharide and IL‐10 was up‐regulated by the cultures. Cell wall extract of L. fermentum MCC 2760 induced the expression of IL‐6 by 5·47‐fold, whereas crude culture filtrate enhanced the expression of IL‐10 by 14·87‐fold compared to LPS control.
Conclusions
The bacterial cultures exhibited strong adhesion and anti‐inflammatory properties.
Significance and Impact of the Study
This is the first report to reveal the role of adhesion markers of L. fermentum and L. delbrueckii by qPCR. The strain‐specific anti‐inflammatory property of native cultures may be useful to alleviate inflammatory conditions and develop a target‐based probiotic.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>29537703</pmid><doi>10.1111/jam.13757</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-0293-1891</orcidid></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Adhesion adhesion potential Adhesion tests Adhesive strength anti‐inflammatory Bile Biotechnology Caco‐2 Carbohydrates Cell culture Cell lines Cell walls Cytokines Filtrate Gene expression IL‐10 Inflammation Lactobacillus Lactobacillus delbrueckii Lactobacillus fermentum lipopolysaccharide Lipopolysaccharides Markers Mucin Pancreatin Probiotics Properties (attributes) Proteins real‐time qPCR Yogurt |
| title | In vitro adhesion and anti‐inflammatory properties of native Lactobacillus fermentum and Lactobacillus delbrueckii spp |
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