Gene expression profile following an oral unsaturated fat load in abdominal obese subjects
Purpose Our aim was to evaluate the postprandial effect of an oral fat load test (OFLT) rich in unsaturated fatty acids on gene expression profile in peripheral blood mononuclear cells (PBMC) from subjects with abdominal obesity as an insulin resistance model and controls. Methods A total of 20 cont...
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| Veröffentlicht in: | European journal of nutrition 2019-04, Vol.58 (3), p.1331-1337 |
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| creator | Garcia-Garcia, A. Bárbara Martinez-Hervas, S. Real, J. T. Marin-Garcia, P. de Marco, G. Priego, A. Martínez-Valls, J. F. Carmena, R. Chaves, F. J. Ascaso, J. F. |
| description | Purpose
Our aim was to evaluate the postprandial effect of an oral fat load test (OFLT) rich in unsaturated fatty acids on gene expression profile in peripheral blood mononuclear cells (PBMC) from subjects with abdominal obesity as an insulin resistance model and controls.
Methods
A total of 20 controls and 20 abdominal obese patients were studied. Metabolic parameters and oxidative stress markers were measured with standardized protocols. The whole gene expression at fasting state and after the OFLT (0, 4 and 8 h) was analysed using human HT-12-v4 expression beadchips, from Illumina.
Results
We found a significant decrease in plasma glucose, insulin and oxidative stress markers in abdominal obese patients and controls. We found beneficial metabolic postprandial gene expression in three genes: FKBP5, DDIT4 and DHRS9. Following an OFLT, the postprandial mRNA expression of FKBP5, and DDIT4 was downregulated while that of DHRS9 was overexpressed, both in nondiabetic normolipidemic subjects and in insulin-resistant subjects with abdominal obesity.
Conclusions
Our results suggest that an OFLT rich in unsaturated fatty acids downregulates the expression of FKBP5, coding for the glucocorticoid receptor pathway, and that of DDIT4, involved in the oxidative stress response. These changes could favourably influence the insulin resistance and oxidative stress status in the postprandial state. |
| doi_str_mv | 10.1007/s00394-018-1659-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2012914049</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2015463040</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-2e1d142ef65657f3671cce7658194e597bcfb3695082b1f9ba0270a146de31f03</originalsourceid><addsrcrecordid>eNp1kE1rHDEMhk1JaNK0PyCXYMill2kkf66PISTbQiCX9NKL8czIYZZZe2PP0Pbfd7abplDoSQI9eiUexs4RPiGAvaoA0qkGcNWg0a5Rb9gpKmkaI1AfvfZgT9i7WjcAIKTBt-xEOC3ACHvKvq0pEacfu0K1DjnxXclxGInHPI75-5CeeEg8lzDyOdUwzSVM1PMYJj7m0PMh8dD2eTukhcgtVeJ1bjfUTfU9O45hrPThpZ6xr3e3jzefm_uH9Zeb6_umk1ZMjSDsUQmKRhttozQWu46s0St0irSzbRdbaZyGlWgxujaAsBBQmZ4kRpBn7OMhd3n9eaY6-e1QOxrHkCjP1QtA4VCBcgt6-Q-6yXNZXv9NaWUkqH0gHqiu5FoLRb8rwzaUnx7B78X7g3i_iPd78V4tOxcvyXO7pf5144_pBRAHoC6j9ETl7-n_p_4C-1ONCA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2015463040</pqid></control><display><type>article</type><title>Gene expression profile following an oral unsaturated fat load in abdominal obese subjects</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Garcia-Garcia, A. Bárbara ; Martinez-Hervas, S. ; Real, J. T. ; Marin-Garcia, P. ; de Marco, G. ; Priego, A. ; Martínez-Valls, J. F. ; Carmena, R. ; Chaves, F. J. ; Ascaso, J. F.</creator><creatorcontrib>Garcia-Garcia, A. Bárbara ; Martinez-Hervas, S. ; Real, J. T. ; Marin-Garcia, P. ; de Marco, G. ; Priego, A. ; Martínez-Valls, J. F. ; Carmena, R. ; Chaves, F. J. ; Ascaso, J. F.</creatorcontrib><description>Purpose
Our aim was to evaluate the postprandial effect of an oral fat load test (OFLT) rich in unsaturated fatty acids on gene expression profile in peripheral blood mononuclear cells (PBMC) from subjects with abdominal obesity as an insulin resistance model and controls.
Methods
A total of 20 controls and 20 abdominal obese patients were studied. Metabolic parameters and oxidative stress markers were measured with standardized protocols. The whole gene expression at fasting state and after the OFLT (0, 4 and 8 h) was analysed using human HT-12-v4 expression beadchips, from Illumina.
Results
We found a significant decrease in plasma glucose, insulin and oxidative stress markers in abdominal obese patients and controls. We found beneficial metabolic postprandial gene expression in three genes: FKBP5, DDIT4 and DHRS9. Following an OFLT, the postprandial mRNA expression of FKBP5, and DDIT4 was downregulated while that of DHRS9 was overexpressed, both in nondiabetic normolipidemic subjects and in insulin-resistant subjects with abdominal obesity.
Conclusions
Our results suggest that an OFLT rich in unsaturated fatty acids downregulates the expression of FKBP5, coding for the glucocorticoid receptor pathway, and that of DDIT4, involved in the oxidative stress response. These changes could favourably influence the insulin resistance and oxidative stress status in the postprandial state.</description><identifier>ISSN: 1436-6207</identifier><identifier>EISSN: 1436-6215</identifier><identifier>DOI: 10.1007/s00394-018-1659-4</identifier><identifier>PMID: 29520627</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Abdomen ; Administration, Oral ; Adolescent ; Adult ; Aged ; Blood Glucose - metabolism ; Cellular stress response ; Chemistry ; Chemistry and Materials Science ; Fat load ; Fats, Unsaturated - administration & dosage ; Fats, Unsaturated - pharmacology ; Fatty acids ; Female ; Gene expression ; Gene Expression Profiling - methods ; Glucocorticoids ; Humans ; Insulin ; Insulin - blood ; Insulin resistance ; Leukocytes (mononuclear) ; Leukocytes, Mononuclear - metabolism ; Male ; Metabolism ; Middle Aged ; Nutrition ; Obesity ; Obesity, Abdominal - genetics ; Obesity, Abdominal - metabolism ; Original Contribution ; Oxidative Stress ; Peripheral blood mononuclear cells ; Postprandial Period ; Young Adult</subject><ispartof>European journal of nutrition, 2019-04, Vol.58 (3), p.1331-1337</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>European Journal of Nutrition is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-2e1d142ef65657f3671cce7658194e597bcfb3695082b1f9ba0270a146de31f03</citedby><cites>FETCH-LOGICAL-c372t-2e1d142ef65657f3671cce7658194e597bcfb3695082b1f9ba0270a146de31f03</cites><orcidid>0000-0002-6775-2034</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00394-018-1659-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00394-018-1659-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29520627$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garcia-Garcia, A. Bárbara</creatorcontrib><creatorcontrib>Martinez-Hervas, S.</creatorcontrib><creatorcontrib>Real, J. T.</creatorcontrib><creatorcontrib>Marin-Garcia, P.</creatorcontrib><creatorcontrib>de Marco, G.</creatorcontrib><creatorcontrib>Priego, A.</creatorcontrib><creatorcontrib>Martínez-Valls, J. F.</creatorcontrib><creatorcontrib>Carmena, R.</creatorcontrib><creatorcontrib>Chaves, F. J.</creatorcontrib><creatorcontrib>Ascaso, J. F.</creatorcontrib><title>Gene expression profile following an oral unsaturated fat load in abdominal obese subjects</title><title>European journal of nutrition</title><addtitle>Eur J Nutr</addtitle><addtitle>Eur J Nutr</addtitle><description>Purpose
Our aim was to evaluate the postprandial effect of an oral fat load test (OFLT) rich in unsaturated fatty acids on gene expression profile in peripheral blood mononuclear cells (PBMC) from subjects with abdominal obesity as an insulin resistance model and controls.
Methods
A total of 20 controls and 20 abdominal obese patients were studied. Metabolic parameters and oxidative stress markers were measured with standardized protocols. The whole gene expression at fasting state and after the OFLT (0, 4 and 8 h) was analysed using human HT-12-v4 expression beadchips, from Illumina.
Results
We found a significant decrease in plasma glucose, insulin and oxidative stress markers in abdominal obese patients and controls. We found beneficial metabolic postprandial gene expression in three genes: FKBP5, DDIT4 and DHRS9. Following an OFLT, the postprandial mRNA expression of FKBP5, and DDIT4 was downregulated while that of DHRS9 was overexpressed, both in nondiabetic normolipidemic subjects and in insulin-resistant subjects with abdominal obesity.
Conclusions
Our results suggest that an OFLT rich in unsaturated fatty acids downregulates the expression of FKBP5, coding for the glucocorticoid receptor pathway, and that of DDIT4, involved in the oxidative stress response. These changes could favourably influence the insulin resistance and oxidative stress status in the postprandial state.</description><subject>Abdomen</subject><subject>Administration, Oral</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Blood Glucose - metabolism</subject><subject>Cellular stress response</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Fat load</subject><subject>Fats, Unsaturated - administration & dosage</subject><subject>Fats, Unsaturated - pharmacology</subject><subject>Fatty acids</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Profiling - methods</subject><subject>Glucocorticoids</subject><subject>Humans</subject><subject>Insulin</subject><subject>Insulin - blood</subject><subject>Insulin resistance</subject><subject>Leukocytes (mononuclear)</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Male</subject><subject>Metabolism</subject><subject>Middle Aged</subject><subject>Nutrition</subject><subject>Obesity</subject><subject>Obesity, Abdominal - genetics</subject><subject>Obesity, Abdominal - metabolism</subject><subject>Original Contribution</subject><subject>Oxidative Stress</subject><subject>Peripheral blood mononuclear cells</subject><subject>Postprandial Period</subject><subject>Young Adult</subject><issn>1436-6207</issn><issn>1436-6215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kE1rHDEMhk1JaNK0PyCXYMill2kkf66PISTbQiCX9NKL8czIYZZZe2PP0Pbfd7abplDoSQI9eiUexs4RPiGAvaoA0qkGcNWg0a5Rb9gpKmkaI1AfvfZgT9i7WjcAIKTBt-xEOC3ACHvKvq0pEacfu0K1DjnxXclxGInHPI75-5CeeEg8lzDyOdUwzSVM1PMYJj7m0PMh8dD2eTukhcgtVeJ1bjfUTfU9O45hrPThpZ6xr3e3jzefm_uH9Zeb6_umk1ZMjSDsUQmKRhttozQWu46s0St0irSzbRdbaZyGlWgxujaAsBBQmZ4kRpBn7OMhd3n9eaY6-e1QOxrHkCjP1QtA4VCBcgt6-Q-6yXNZXv9NaWUkqH0gHqiu5FoLRb8rwzaUnx7B78X7g3i_iPd78V4tOxcvyXO7pf5144_pBRAHoC6j9ETl7-n_p_4C-1ONCA</recordid><startdate>20190401</startdate><enddate>20190401</enddate><creator>Garcia-Garcia, A. Bárbara</creator><creator>Martinez-Hervas, S.</creator><creator>Real, J. T.</creator><creator>Marin-Garcia, P.</creator><creator>de Marco, G.</creator><creator>Priego, A.</creator><creator>Martínez-Valls, J. F.</creator><creator>Carmena, R.</creator><creator>Chaves, F. J.</creator><creator>Ascaso, J. F.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RQ</scope><scope>7RV</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6775-2034</orcidid></search><sort><creationdate>20190401</creationdate><title>Gene expression profile following an oral unsaturated fat load in abdominal obese subjects</title><author>Garcia-Garcia, A. Bárbara ; Martinez-Hervas, S. ; Real, J. T. ; Marin-Garcia, P. ; de Marco, G. ; Priego, A. ; Martínez-Valls, J. F. ; Carmena, R. ; Chaves, F. J. ; Ascaso, J. F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-2e1d142ef65657f3671cce7658194e597bcfb3695082b1f9ba0270a146de31f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Abdomen</topic><topic>Administration, Oral</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Blood Glucose - metabolism</topic><topic>Cellular stress response</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Fat load</topic><topic>Fats, Unsaturated - administration & dosage</topic><topic>Fats, Unsaturated - pharmacology</topic><topic>Fatty acids</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Profiling - methods</topic><topic>Glucocorticoids</topic><topic>Humans</topic><topic>Insulin</topic><topic>Insulin - blood</topic><topic>Insulin resistance</topic><topic>Leukocytes (mononuclear)</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Male</topic><topic>Metabolism</topic><topic>Middle Aged</topic><topic>Nutrition</topic><topic>Obesity</topic><topic>Obesity, Abdominal - genetics</topic><topic>Obesity, Abdominal - metabolism</topic><topic>Original Contribution</topic><topic>Oxidative Stress</topic><topic>Peripheral blood mononuclear cells</topic><topic>Postprandial Period</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garcia-Garcia, A. Bárbara</creatorcontrib><creatorcontrib>Martinez-Hervas, S.</creatorcontrib><creatorcontrib>Real, J. T.</creatorcontrib><creatorcontrib>Marin-Garcia, P.</creatorcontrib><creatorcontrib>de Marco, G.</creatorcontrib><creatorcontrib>Priego, A.</creatorcontrib><creatorcontrib>Martínez-Valls, J. F.</creatorcontrib><creatorcontrib>Carmena, R.</creatorcontrib><creatorcontrib>Chaves, F. J.</creatorcontrib><creatorcontrib>Ascaso, J. 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Bárbara</au><au>Martinez-Hervas, S.</au><au>Real, J. T.</au><au>Marin-Garcia, P.</au><au>de Marco, G.</au><au>Priego, A.</au><au>Martínez-Valls, J. F.</au><au>Carmena, R.</au><au>Chaves, F. J.</au><au>Ascaso, J. F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene expression profile following an oral unsaturated fat load in abdominal obese subjects</atitle><jtitle>European journal of nutrition</jtitle><stitle>Eur J Nutr</stitle><addtitle>Eur J Nutr</addtitle><date>2019-04-01</date><risdate>2019</risdate><volume>58</volume><issue>3</issue><spage>1331</spage><epage>1337</epage><pages>1331-1337</pages><issn>1436-6207</issn><eissn>1436-6215</eissn><abstract>Purpose
Our aim was to evaluate the postprandial effect of an oral fat load test (OFLT) rich in unsaturated fatty acids on gene expression profile in peripheral blood mononuclear cells (PBMC) from subjects with abdominal obesity as an insulin resistance model and controls.
Methods
A total of 20 controls and 20 abdominal obese patients were studied. Metabolic parameters and oxidative stress markers were measured with standardized protocols. The whole gene expression at fasting state and after the OFLT (0, 4 and 8 h) was analysed using human HT-12-v4 expression beadchips, from Illumina.
Results
We found a significant decrease in plasma glucose, insulin and oxidative stress markers in abdominal obese patients and controls. We found beneficial metabolic postprandial gene expression in three genes: FKBP5, DDIT4 and DHRS9. Following an OFLT, the postprandial mRNA expression of FKBP5, and DDIT4 was downregulated while that of DHRS9 was overexpressed, both in nondiabetic normolipidemic subjects and in insulin-resistant subjects with abdominal obesity.
Conclusions
Our results suggest that an OFLT rich in unsaturated fatty acids downregulates the expression of FKBP5, coding for the glucocorticoid receptor pathway, and that of DDIT4, involved in the oxidative stress response. These changes could favourably influence the insulin resistance and oxidative stress status in the postprandial state.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29520627</pmid><doi>10.1007/s00394-018-1659-4</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-6775-2034</orcidid></addata></record> |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Abdomen Administration, Oral Adolescent Adult Aged Blood Glucose - metabolism Cellular stress response Chemistry Chemistry and Materials Science Fat load Fats, Unsaturated - administration & dosage Fats, Unsaturated - pharmacology Fatty acids Female Gene expression Gene Expression Profiling - methods Glucocorticoids Humans Insulin Insulin - blood Insulin resistance Leukocytes (mononuclear) Leukocytes, Mononuclear - metabolism Male Metabolism Middle Aged Nutrition Obesity Obesity, Abdominal - genetics Obesity, Abdominal - metabolism Original Contribution Oxidative Stress Peripheral blood mononuclear cells Postprandial Period Young Adult |
| title | Gene expression profile following an oral unsaturated fat load in abdominal obese subjects |
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