Complete solubilization of cartilage using the heat-stable protease thermolysin for comprehensive GAG analysis

Articular cartilage comprises collagens, proteoglycans, and glycosaminoglycans (GAGs) together with water, in hyaline matrixes. Articular cartilage is resistant to proteolytic solubilization for comprehensive GAG analyses partly because of assemblies of collagen fibers with thermolabile hydrogen bon...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Analytical biochemistry 2018-05, Vol.548, p.115-118
Hauptverfasser:	Osago, Harumi, Kobayashi-Miura, Mikiko, Hamasaki, Yoshifumi, Hara, Nobumasa, Hiyoshi, Mineyoshi, Tsuchiya, Mikako
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cartilage Cartilage - chemistry Cattle Collagen Disaccharides - analysis Disaccharides - chemistry Glycosaminoglycan Glycosaminoglycans - analysis Glycosaminoglycans - chemistry Heat-stable protease Hot Temperature Hydrogen bond Thermolysin Thermolysin - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!