Evaluation of Prolidase Activity, Oxidative Stress, and Antioxidant Enzyme Levels in Testicular and Penile Tissues after Human Chorionic Gonadotropin Treatment in Rats by Predicting Infertility and Erectile Dysfunction
Objectives: Prolidase plays a vital role in collagen turnover, matrix remodeling, and cell growth. We aimed to evaluate the association between treatment with chorionic gonadotropin and infertility and erectile dysfunction by investigating tissue prolidase activity, oxidative stress, and levels of a...
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| Veröffentlicht in: | Medical principles and practice 2018-01, Vol.27 (3), p.217-221 |
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| creator | Pirinççi, Necip Yildirim, Serkan Taş, Abuzer Kuşcu, Yağmur Ozan, Tunç Fırdolaş, Fatih Demir, Halit Karakeci, Ahmet |
| description | Objectives: Prolidase plays a vital role in collagen turnover, matrix remodeling, and cell growth. We aimed to evaluate the association between treatment with chorionic gonadotropin and infertility and erectile dysfunction by investigating tissue prolidase activity, oxidative stress, and levels of antioxidant enzymes. Materials and Methods: The 16 male Wistar albino rats used in this study were randomly divided into 2 groups: rats treated with human chorionic gonadotropin (hCG) and control rats (n = 8 in each group). The rats in the hCG group were subcutaneously injected with 50 IU hCG daily for 15 days, while the rats in the control group were subcutaneously injected isotonic saline. All of the rats were sacrificed by a lethal overdose of sodium pentobarbital at the first month after hCG administration. Prolidase activity and levels of malonyl aldehyde, glutathione reductase, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were estimated in the testicular and penile tissue. The testicles and penis were transversely dissected and placed in formalin. Results: Levels of prolidase and malonyl aldehyde in the testicular and penile tissues were significantly higher in the hCG group than in the control group (p < 0.001), while levels of glutathione reductase, SOD, GSH-Px, and CAT were significantly lower in the hCG group than in the control group (p < 0.001). Conclusions: In this study, we observed that treatment with hCG increased prolidase activity and oxidative stress and decreased the antioxidant capacity of penile and testicular tissues; therefore, this may affect fertility and erectile function. |
| doi_str_mv | 10.1159/000488109 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_karge</sourceid><recordid>TN_cdi_proquest_miscellaneous_2011270551</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2011270551</sourcerecordid><originalsourceid>FETCH-LOGICAL-c452t-c7cc9aa882f52f39f7e8c4f39c8f96a4a5ea29b0c196eb6dd73e8e4d7fd71f343</originalsourceid><addsrcrecordid>eNptkktvEzEUhUcIREthwR4hS2xAasCe92yQohDaSkGNIKytG8916jJjB9sTEX4qv4abJoSHWPlxP59zbN8keSr4ayGK5g3nPK9rwZt7yanI02zERSHu05wLMaqKSpwkj0K4JazOMv4wOUmbgpecN6fJj-kGugGicZY5zebedaaFgGysotmYuD1n199ohxbIPkWPIZwzsC0bWzqzq9jIpvb7tkc2ww12gRnLFhiiUUMH_o6dozUdsoUJYcDAQEf07HLowbLJjfPkbRS7cBZaF71b7wQ8QuyRtGnxEWJgyy2Fw9ZQLLtiV1ajj6ajgHcOU49UII9326AHq3b3eZw80NAFfHIYz5LP76eLyeVodn1xNRnPRiov0jhSlVINQF2nukh11ugKa5XTRNW6KSGHAiFtllyJpsRl2bZVhjXmbaXbSugsz86St3vd9bDssVWU2kMn19704LfSgZF_V6y5kSu3kSUv0yrLSODlQcC7r_RAUfYmKOw6sOiGIFP6xrTiRSEIffEPeusGb-l6MhWiEgXP8pKoV3tKeReCR30MI7jcdYw8dgyxz_9MfyR_tchvyy_gV-iPwIf5fC8h160m6tl_qYPLT1xv1t4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2117150346</pqid></control><display><type>article</type><title>Evaluation of Prolidase Activity, Oxidative Stress, and Antioxidant Enzyme Levels in Testicular and Penile Tissues after Human Chorionic Gonadotropin Treatment in Rats by Predicting Infertility and Erectile Dysfunction</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Karger Open Access</source><creator>Pirinççi, Necip ; Yildirim, Serkan ; Taş, Abuzer ; Kuşcu, Yağmur ; Ozan, Tunç ; Fırdolaş, Fatih ; Demir, Halit ; Karakeci, Ahmet</creator><creatorcontrib>Pirinççi, Necip ; Yildirim, Serkan ; Taş, Abuzer ; Kuşcu, Yağmur ; Ozan, Tunç ; Fırdolaş, Fatih ; Demir, Halit ; Karakeci, Ahmet</creatorcontrib><description>Objectives: Prolidase plays a vital role in collagen turnover, matrix remodeling, and cell growth. We aimed to evaluate the association between treatment with chorionic gonadotropin and infertility and erectile dysfunction by investigating tissue prolidase activity, oxidative stress, and levels of antioxidant enzymes. Materials and Methods: The 16 male Wistar albino rats used in this study were randomly divided into 2 groups: rats treated with human chorionic gonadotropin (hCG) and control rats (n = 8 in each group). The rats in the hCG group were subcutaneously injected with 50 IU hCG daily for 15 days, while the rats in the control group were subcutaneously injected isotonic saline. All of the rats were sacrificed by a lethal overdose of sodium pentobarbital at the first month after hCG administration. Prolidase activity and levels of malonyl aldehyde, glutathione reductase, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were estimated in the testicular and penile tissue. The testicles and penis were transversely dissected and placed in formalin. Results: Levels of prolidase and malonyl aldehyde in the testicular and penile tissues were significantly higher in the hCG group than in the control group (p < 0.001), while levels of glutathione reductase, SOD, GSH-Px, and CAT were significantly lower in the hCG group than in the control group (p < 0.001). Conclusions: In this study, we observed that treatment with hCG increased prolidase activity and oxidative stress and decreased the antioxidant capacity of penile and testicular tissues; therefore, this may affect fertility and erectile function.</description><identifier>ISSN: 1011-7571</identifier><identifier>EISSN: 1423-0151</identifier><identifier>DOI: 10.1159/000488109</identifier><identifier>PMID: 29506009</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Acids ; Animals ; Antioxidants ; Antioxidants - pharmacology ; Cardiovascular disease ; Chorionic Gonadotropin - pharmacology ; Diabetes ; Drug dosages ; Enzymes ; Erectile dysfunction ; Glutathione Peroxidase - metabolism ; Infertility ; Lipid Peroxidation - drug effects ; Male ; Original Paper ; Oxidative stress ; Oxidative Stress - drug effects ; Physiology ; Rats ; Rodents ; Testis - drug effects ; Vasoconstrictor Agents - pharmacology</subject><ispartof>Medical principles and practice, 2018-01, Vol.27 (3), p.217-221</ispartof><rights>2018 The Author(s) Published by S. Karger AG, Basel</rights><rights>2018 The Author(s) Published by S. Karger AG, Basel.</rights><rights>Copyright © 2018 by S. Karger AG, Basel 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-c7cc9aa882f52f39f7e8c4f39c8f96a4a5ea29b0c196eb6dd73e8e4d7fd71f343</citedby><cites>FETCH-LOGICAL-c452t-c7cc9aa882f52f39f7e8c4f39c8f96a4a5ea29b0c196eb6dd73e8e4d7fd71f343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062733/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062733/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27612,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29506009$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pirinççi, Necip</creatorcontrib><creatorcontrib>Yildirim, Serkan</creatorcontrib><creatorcontrib>Taş, Abuzer</creatorcontrib><creatorcontrib>Kuşcu, Yağmur</creatorcontrib><creatorcontrib>Ozan, Tunç</creatorcontrib><creatorcontrib>Fırdolaş, Fatih</creatorcontrib><creatorcontrib>Demir, Halit</creatorcontrib><creatorcontrib>Karakeci, Ahmet</creatorcontrib><title>Evaluation of Prolidase Activity, Oxidative Stress, and Antioxidant Enzyme Levels in Testicular and Penile Tissues after Human Chorionic Gonadotropin Treatment in Rats by Predicting Infertility and Erectile Dysfunction</title><title>Medical principles and practice</title><addtitle>Med Princ Pract</addtitle><description>Objectives: Prolidase plays a vital role in collagen turnover, matrix remodeling, and cell growth. We aimed to evaluate the association between treatment with chorionic gonadotropin and infertility and erectile dysfunction by investigating tissue prolidase activity, oxidative stress, and levels of antioxidant enzymes. Materials and Methods: The 16 male Wistar albino rats used in this study were randomly divided into 2 groups: rats treated with human chorionic gonadotropin (hCG) and control rats (n = 8 in each group). The rats in the hCG group were subcutaneously injected with 50 IU hCG daily for 15 days, while the rats in the control group were subcutaneously injected isotonic saline. All of the rats were sacrificed by a lethal overdose of sodium pentobarbital at the first month after hCG administration. Prolidase activity and levels of malonyl aldehyde, glutathione reductase, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were estimated in the testicular and penile tissue. The testicles and penis were transversely dissected and placed in formalin. Results: Levels of prolidase and malonyl aldehyde in the testicular and penile tissues were significantly higher in the hCG group than in the control group (p < 0.001), while levels of glutathione reductase, SOD, GSH-Px, and CAT were significantly lower in the hCG group than in the control group (p < 0.001). Conclusions: In this study, we observed that treatment with hCG increased prolidase activity and oxidative stress and decreased the antioxidant capacity of penile and testicular tissues; therefore, this may affect fertility and erectile function.</description><subject>Acids</subject><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Cardiovascular disease</subject><subject>Chorionic Gonadotropin - pharmacology</subject><subject>Diabetes</subject><subject>Drug dosages</subject><subject>Enzymes</subject><subject>Erectile dysfunction</subject><subject>Glutathione Peroxidase - metabolism</subject><subject>Infertility</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Male</subject><subject>Original Paper</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Physiology</subject><subject>Rats</subject><subject>Rodents</subject><subject>Testis - drug effects</subject><subject>Vasoconstrictor Agents - pharmacology</subject><issn>1011-7571</issn><issn>1423-0151</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>M--</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptkktvEzEUhUcIREthwR4hS2xAasCe92yQohDaSkGNIKytG8916jJjB9sTEX4qv4abJoSHWPlxP59zbN8keSr4ayGK5g3nPK9rwZt7yanI02zERSHu05wLMaqKSpwkj0K4JazOMv4wOUmbgpecN6fJj-kGugGicZY5zebedaaFgGysotmYuD1n199ohxbIPkWPIZwzsC0bWzqzq9jIpvb7tkc2ww12gRnLFhiiUUMH_o6dozUdsoUJYcDAQEf07HLowbLJjfPkbRS7cBZaF71b7wQ8QuyRtGnxEWJgyy2Fw9ZQLLtiV1ajj6ajgHcOU49UII9326AHq3b3eZw80NAFfHIYz5LP76eLyeVodn1xNRnPRiov0jhSlVINQF2nukh11ugKa5XTRNW6KSGHAiFtllyJpsRl2bZVhjXmbaXbSugsz86St3vd9bDssVWU2kMn19704LfSgZF_V6y5kSu3kSUv0yrLSODlQcC7r_RAUfYmKOw6sOiGIFP6xrTiRSEIffEPeusGb-l6MhWiEgXP8pKoV3tKeReCR30MI7jcdYw8dgyxz_9MfyR_tchvyy_gV-iPwIf5fC8h160m6tl_qYPLT1xv1t4</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Pirinççi, Necip</creator><creator>Yildirim, Serkan</creator><creator>Taş, Abuzer</creator><creator>Kuşcu, Yağmur</creator><creator>Ozan, Tunç</creator><creator>Fırdolaş, Fatih</creator><creator>Demir, Halit</creator><creator>Karakeci, Ahmet</creator><general>S. 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Karakeci, Ahmet</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-c7cc9aa882f52f39f7e8c4f39c8f96a4a5ea29b0c196eb6dd73e8e4d7fd71f343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acids</topic><topic>Animals</topic><topic>Antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>Cardiovascular disease</topic><topic>Chorionic Gonadotropin - pharmacology</topic><topic>Diabetes</topic><topic>Drug dosages</topic><topic>Enzymes</topic><topic>Erectile dysfunction</topic><topic>Glutathione Peroxidase - metabolism</topic><topic>Infertility</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Male</topic><topic>Original Paper</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Physiology</topic><topic>Rats</topic><topic>Rodents</topic><topic>Testis - drug effects</topic><topic>Vasoconstrictor Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pirinççi, Necip</creatorcontrib><creatorcontrib>Yildirim, Serkan</creatorcontrib><creatorcontrib>Taş, Abuzer</creatorcontrib><creatorcontrib>Kuşcu, Yağmur</creatorcontrib><creatorcontrib>Ozan, Tunç</creatorcontrib><creatorcontrib>Fırdolaş, Fatih</creatorcontrib><creatorcontrib>Demir, Halit</creatorcontrib><creatorcontrib>Karakeci, Ahmet</creatorcontrib><collection>Karger Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Medical principles and practice</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pirinççi, Necip</au><au>Yildirim, Serkan</au><au>Taş, Abuzer</au><au>Kuşcu, Yağmur</au><au>Ozan, Tunç</au><au>Fırdolaş, Fatih</au><au>Demir, Halit</au><au>Karakeci, Ahmet</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Prolidase Activity, Oxidative Stress, and Antioxidant Enzyme Levels in Testicular and Penile Tissues after Human Chorionic Gonadotropin Treatment in Rats by Predicting Infertility and Erectile Dysfunction</atitle><jtitle>Medical principles and practice</jtitle><addtitle>Med Princ Pract</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>27</volume><issue>3</issue><spage>217</spage><epage>221</epage><pages>217-221</pages><issn>1011-7571</issn><eissn>1423-0151</eissn><abstract>Objectives: Prolidase plays a vital role in collagen turnover, matrix remodeling, and cell growth. We aimed to evaluate the association between treatment with chorionic gonadotropin and infertility and erectile dysfunction by investigating tissue prolidase activity, oxidative stress, and levels of antioxidant enzymes. Materials and Methods: The 16 male Wistar albino rats used in this study were randomly divided into 2 groups: rats treated with human chorionic gonadotropin (hCG) and control rats (n = 8 in each group). The rats in the hCG group were subcutaneously injected with 50 IU hCG daily for 15 days, while the rats in the control group were subcutaneously injected isotonic saline. All of the rats were sacrificed by a lethal overdose of sodium pentobarbital at the first month after hCG administration. Prolidase activity and levels of malonyl aldehyde, glutathione reductase, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were estimated in the testicular and penile tissue. The testicles and penis were transversely dissected and placed in formalin. Results: Levels of prolidase and malonyl aldehyde in the testicular and penile tissues were significantly higher in the hCG group than in the control group (p < 0.001), while levels of glutathione reductase, SOD, GSH-Px, and CAT were significantly lower in the hCG group than in the control group (p < 0.001). Conclusions: In this study, we observed that treatment with hCG increased prolidase activity and oxidative stress and decreased the antioxidant capacity of penile and testicular tissues; therefore, this may affect fertility and erectile function.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>29506009</pmid><doi>10.1159/000488109</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Karger Open Access |
| subjects | Acids Animals Antioxidants Antioxidants - pharmacology Cardiovascular disease Chorionic Gonadotropin - pharmacology Diabetes Drug dosages Enzymes Erectile dysfunction Glutathione Peroxidase - metabolism Infertility Lipid Peroxidation - drug effects Male Original Paper Oxidative stress Oxidative Stress - drug effects Physiology Rats Rodents Testis - drug effects Vasoconstrictor Agents - pharmacology |
| title | Evaluation of Prolidase Activity, Oxidative Stress, and Antioxidant Enzyme Levels in Testicular and Penile Tissues after Human Chorionic Gonadotropin Treatment in Rats by Predicting Infertility and Erectile Dysfunction |
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