Preparation of Human Olfactory Ensheathing Cells for the Therapy of Spinal Cord Injuries

We developed an optimal protocol for preparing and culturing of olfactory ensheathing cells from human olfactory mucosa. Using this protocol, we obtained a culture enriched with human olfactory ensheathing cells. Immunofluorescence analysis by simultaneous expression of GFAP and p75NTR markers showe...

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Veröffentlicht in:	Bulletin of experimental biology and medicine 2018-03, Vol.164 (4), p.523-527
Hauptverfasser:	Voronova, А. D., Stepanova, O. V., Valikhov, M. P., Chadin, A. V., Dvornikov, А. S., Reshetov, I. V., Chekhonin, V. P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biomarkers - metabolism Biomedical and Life Sciences Biomedicine Cell Biology Cell culture Cell Proliferation Cell Technologies in Biology and Medicine Cell- and Tissue-Based Therapy - methods Gene Expression Glial fibrillary acidic protein Glial Fibrillary Acidic Protein - genetics Glial Fibrillary Acidic Protein - metabolism Humans Immunofluorescence Injuries Internal Medicine Laboratory Medicine Mucosa Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Olfactory Bulb - cytology Olfactory Bulb - metabolism Olfactory Bulb - surgery Olfactory ensheathing cells Olfactory epithelium Olfactory Mucosa - cytology Olfactory Mucosa - metabolism Olfactory Mucosa - surgery Pathology Primary Cell Culture - methods Receptors, Nerve Growth Factor - genetics Receptors, Nerve Growth Factor - metabolism Spinal cord injuries Spinal Cord Injuries - therapy
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Zusammenfassung:	We developed an optimal protocol for preparing and culturing of olfactory ensheathing cells from human olfactory mucosa. Using this protocol, we obtained a culture enriched with human olfactory ensheathing cells. Immunofluorescence analysis by simultaneous expression of GFAP and p75NTR markers showed that the content of ensheathing cells was maximum in passage 3 and 4 cultures (94 and 89.5%, respectively). The developed protocol can be recommended for obtaining autologous preparations of human ensheathing cells for cell therapy of spinal cord injuries.
ISSN:	0007-4888 1573-8221
DOI:	10.1007/s10517-018-4025-x